Adenosine Triphosphatases

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  Subject Areas on Research

  • 2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition. 
  • A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch. 
  • A kinetic study of the binding of an ADP fluorescent analog to mitochondrial ATPase. 
  • A model for fast axonal transport. 
  • A multimember kinesin gene family in Drosophila. 
  • A naturally occurring hPMS2 mutation can confer a dominant negative mutator phenotype. 
  • A novel function of VCP (valosin-containing protein; p97) in the control of N-glycosylation of proteins in the endoplasmic reticulum. 
  • A point mutation in the microtubule binding region of the Ncd motor protein reduces motor velocity. 
  • A second leaky splice-site mutation in the spastin gene. 
  • ATP hydrolysis by a CFTR domain: pharmacology and effects of G551D mutation. 
  • Abasic sites in the transcribed strand of yeast DNA are removed by transcription-coupled nucleotide excision repair. 
  • Activation of G551D CFTR channel with MPB-91: regulation by ATPase activity and phosphorylation. 
  • Activation of beef heart mitochondrial adenosine triphosphatase by 2,4-dinitrophenol. 
  • Adenocarcinoma of the kidney. II. Enzyme histochemistry of renal adenocarcinomas induced in rats by N-(4'-fluoro-4-biphenylyl)acetamide. 
  • Affinity chromatography of H+-translocating adenosine triphosphatase isolated by chloroform extraction of Rhodospirillum rubrum chromatophores. Modification of binding affinity by divalent cations and activating anions. 
  • Alternative method for quantitative enzyme histochemistry of muscle fibers. Application of photographic densitometry combined with atomic absorption spectrophotometry. 
  • Analysis of DNA mismatch repair proteins in human medulloblastoma. 
  • Analysis of the individual regulatory components of the IncFII plasmid replication control system. 
  • Angiostatin binds ATP synthase on the surface of human endothelial cells. 
  • Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes. 
  • Assembly and molecular activities of the MutS tetramer. 
  • Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. 
  • Atomic force microscopy captures the initiation of methyl-directed DNA mismatch repair. 
  • Atomistic basis of force generation, translocation, and coordination in a viral genome packaging motor. 
  • Autophagy in age-related macular degeneration. 
  • Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins. 
  • Bidirectional excision in methyl-directed mismatch repair. 
  • Bimodal activation of SMC ATPase by intra- and inter-molecular interactions. 
  • Biochemical and cytochemical comparison of surface membranes from normal and dystrophic chickens. 
  • Biological cross-reactivity of rat testis phosphodiesterase activator protein and rabbit skeletal muscle troponin-C. 
  • Blebbistatin, a novel inhibitor of myosin II ATPase activity, increases aqueous humor outflow facility in perfused enucleated porcine eyes. 
  • Calcium accumulation by isolated sarcoplasmic reticulum of skeletal muscle during development in tissue culture. 
  • Calmodulin in endocrine cells and its multiple roles in hormone action. 
  • Cardiac copper deficiency activates a systemic signaling mechanism that communicates with the copper acquisition and storage organs. 
  • Centronuclear myopathy: histochemistry and electron microscopy. Report of two cases. 
  • Centrosome and spindle function of the Drosophila Ncd microtubule motor visualized in live embryos using Ncd-GFP fusion proteins. 
  • Characterization of prenylcysteines that interact with P-glycoprotein and inhibit drug transport in tumor cells. 
  • Characterization of sulfhydryl groups on chloroplast coupling factor 1 exposed by heat activation. 
  • Chemical cross-linking studies of chloroplast coupling factor 1. 
  • Chemical modification and fluorescence studies of chloroplast coupling factor. 
  • Chromatin association of rad17 is required for an ataxia telangiectasia and rad-related kinase-mediated S-phase checkpoint in response to low-dose ultraviolet radiation. 
  • Chronic suppression of heart-failure progression by a pseudophosphorylated mutant of phospholamban via in vivo cardiac rAAV gene delivery. 
  • Cisplatin and adriamycin resistance are associated with MutLalpha and mismatch repair deficiency in an ovarian tumor cell line. 
  • Cold temperature improves mobility and survival in Drosophila models of autosomal-dominant hereditary spastic paraplegia (AD-HSP). 
  • Competition of hydrophobic peptides, cytotoxic drugs, and chemosensitizers on a common P-glycoprotein pharmacophore as revealed by its ATPase activity. 
  • Condensin and cohesin display different arm conformations with characteristic hinge angles. 
  • Conserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity. 
  • Control of a biomolecular motor-powered nanodevice with an engineered chemical switch. 
  • Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension. 
  • Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7. 
  • Cyclic adenosine 3',5' monophosphate, calcium and protein phosphorylation in flagellar motility. 
  • Cytoskeletal dependence of adenosine triphosphate release by human trabecular meshwork cells. 
  • DNA chain length dependence of formation and dynamics of hMutSalpha.hMutLalpha.heteroduplex complexes. 
  • DNA-dependent activation of the hMutSalpha ATPase. 
  • Destabilization of the VCP-Ufd1-Npl4 complex is associated with decreased levels of ERAD substrates. 
  • Determinants of molecular motor directionality. 
  • Differential and simultaneous adenosine di- and triphosphate binding by MutS. 
  • Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha. 
  • Disorders of cell volume regulation. I. Effects of inhibition of plasma membrane adenosine triphosphatase with ouabain. 
  • Disruption of a topoisomerase-DNA cleavage complex by a DNA helicase. 
  • Disruption of myofibrillar energy use: dual mechanisms that may contribute to postischemic dysfunction in stunned myocardium. 
  • Dissecting the order of bacteriophage T4 DNA polymerase holoenzyme assembly. 
  • Distinct MutS DNA-binding modes that are differentially modulated by ATP binding and hydrolysis. 
  • Distinction between alkaline phosphatase and Mg2+-ATPase of the human polymorphonuclear leukocyte. 
  • Drosophila spastin regulates synaptic microtubule networks and is required for normal motor function. 
  • Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss. 
  • Effect of cellular senescence on the P2Y-receptor mediated calcium response in trabecular meshwork cells. 
  • Effects of DDT on eggshell quality and calcium adenosine triphosphatase. 
  • Effects of quercetin on magnesium-dependent adenosine triphosphatase and the metabolism of human polymorphonuclear leukocytes. 
  • Energy transfer assays of rat renal cortical endosomal fusion: evidence for superfusion. 
  • Equilibrium binding of nucleotides to beef heart mitochondrial adenosine triphosphatase. 
  • Evidence that a catalytic glutamate and an 'Arginine Toggle' act in concert to mediate ATP hydrolysis and mechanochemical coupling in a viral DNA packaging motor. 
  • Evidence that myosin does not contribute to force production in chromosome movement. 
  • Exome-wide Sequencing Shows Low Mutation Rates and Identifies Novel Mutated Genes in Seminomas. 
  • Experimental basis for separation of membrane vesicles by preparative free-flow electrophoresis. 
  • Expression of five selected human mismatch repair genes simultaneously detected in normal and cancer cell lines by a nonradioactive multiplex reverse transcription-polymerase chain reaction. 
  • Expression of microtubule motor proteins in bacteria for characterization in in vitro motility assays. 
  • Expression, distribution, and biochemistry of human CD39. Role in activation-associated homotypic adhesion of lymphocytes. 
  • Extracellular secretion of pectate lyase by the Erwinia chrysanthemi out pathway is dependent upon Sec-mediated export across the inner membrane. 
  • Flow linear dichroism and electron microscopic analysis of protein-DNA complexes of a mutant UvrB protein that binds to but cannot kink DNA. 
  • Fractionation of transcription factors for RNA polymerase II from Drosophila Kc cell nuclear extracts. 
  • Frameshift intermediates in homopolymer runs are removed efficiently by yeast mismatch repair proteins. 
  • Functional Analysis of the Bacteriophage T4 Rad50 Homolog (gp46) Coiled-coil Domain. 
  • Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia. 
  • Functional units in rainbow trout (Salmo gairdneri) liver: I. Arrangement and histochemical properties of hepatocytes. 
  • Genetic approaches to molecular motors. 
  • Glucagon stimulation of mitochondrial ATPase and potassium ion transport. 
  • HDAC6 controls major cell response pathways to cytotoxic accumulation of protein aggregates. 
  • HLA polymorphisms in African Americans with idiopathic inflammatory myopathy: allelic profiles distinguish patients with different clinical phenotypes and myositis autoantibodies. 
  • HMW-2, the Sertoli cell cytoplasmic dynein from rat testis, is a dimer composed of nearly identical subunits. 
  • HPLC measurement, blood distribution, and pharmacokinetics of oral clotrimazole, potentially useful antisickling agent. 
  • Human MutSalpha recognizes damaged DNA base pairs containing O6-methylguanine, O4-methylthymine, or the cisplatin-d(GpG) adduct. 
  • Human exonuclease 1 and BLM helicase interact to resect DNA and initiate DNA repair. 
  • Hydrolytically deficient MutS E694A is defective in the MutL-dependent activation of MutH and in the mismatch-dependent assembly of the MutS.MutL.heteroduplex complex. 
  • Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency. 
  • Identification and expression analysis of spastin gene mutations in hereditary spastic paraplegia. 
  • Identification of ENA1 as a virulence gene of the human pathogenic fungus Cryptococcus neoformans through signature-tagged insertional mutagenesis. 
  • In vitro inhibition of microsomal calcium atpase from eggshell gland of mallard duck. 
  • In vitro reconstitution of cortical actin assembly sites in budding yeast. 
  • In vitro studies on the bacteriophage P2 terminase system. 
  • Inhibiting clathrin-mediated endocytosis of the leucine-rich G protein-coupled receptor-5 diminishes cell fitness. 
  • Inhibition of DNA helicase II unwinding and ATPase activities by DNA-interacting ligands. Kinetics and specificity. 
  • Inhibition of acanthamoeba actomyosin-II ATPase activity and mechanochemical function by specific monoclonal antibodies. 
  • Inhibition of the proton-translocating adenosine triphosphatase from chromatophores of photosynthetic bacteria by free bivalent cations and adenosine triphosphate [proceedings]. 
  • Initiation of methyl-directed mismatch repair. 
  • Integration of plasmids into the bacteriophage T4 genome. 
  • Interaction of prenylcysteine methyl esters with the multidrug resistance transporter. 
  • Interconversion of two kinetically distinct states of the membrane-bound and solubilised H+-translocating ATPase from Rhodospirillum rubrum. 
  • Interferon-Inducible E3 Ligase RNF213 Facilitates Host-Protective Linear and K63-Linked Ubiquitylation of Toxoplasma gondii Parasitophorous Vacuoles. 
  • Intragenic modifiers of hereditary spastic paraplegia due to spastin gene mutations. 
  • Involvement of the beta clamp in methyl-directed mismatch repair in vitro. 
  • Isolation of MutSbeta from human cells and comparison of the mismatch repair specificities of MutSbeta and MutSalpha. 
  • Katanin p60-like1 promotes microtubule growth and terminal dendrite stability in the larval class IV sensory neurons of Drosophila. 
  • Ligand interactions in the adenosine nucleotide-binding domain of the Hsp90 chaperone, GRP94. I. Evidence for allosteric regulation of ligand binding. 
  • Linkage to a known gene but no mutation identified: comprehensive reanalysis of SPG4 HSP pedigrees reveals large deletions as the sole cause. 
  • Loss of Drosophila melanogaster p21-activated kinase 3 suppresses defects in synapse structure and function caused by spastin mutations. 
  • Mechanical loading stimulates ecto-ATPase activity in human tendon cells. 
  • Mechanism of 5'-directed excision in human mismatch repair. 
  • Mechanisms in eukaryotic mismatch repair. 
  • Mechanisms of DNA-mismatch correction. 
  • Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin. 
  • Membrane Transporters Involved in the Antimicrobial Activities of Pyrithione in Escherichia coli . 
  • Meta-analysis of age at onset in spastin-associated hereditary spastic paraplegia provides no evidence for a correlation with mutational class. 
  • Methyl-directed mismatch repair is bidirectional. 
  • Mining copper transport genes. 
  • Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs. 
  • Mismatch repair proteins and mitotic genome stability. 
  • Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex. 
  • Modulation of MutS ATP hydrolysis by DNA cofactors. 
  • Molecular and physiological alterations in murine ventricular dysfunction. 
  • Molecular dynamics of DNA translocation by FtsK. 
  • Motor proteins. 1: kinesins. 
  • Multifocal anaplastic astrocytoma in a patient with hereditary colorectal cancer, transcobalamin II deficiency, agenesis of the corpus callosum, mental retardation, and inherited PMS2 mutation. 
  • MutS and MutL activate DNA helicase II in a mismatch-dependent manner. 
  • MutS mediates heteroduplex loop formation by a translocation mechanism. 
  • Mutation detection with MutH, MutL, and MutS mismatch repair proteins. 
  • Mutational analysis of ATP7B and genotype-phenotype correlation in Japanese with Wilson's disease. 
  • Mutations in NCAPG2 Cause a Severe Neurodevelopmental Syndrome that Expands the Phenotypic Spectrum of Condensinopathies. 
  • Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA Coprotease. 
  • Myosin from human erythrocytes. 
  • Normal spastin gene dosage is specifically required for axon regeneration. 
  • Novel bacterial P-type ATPases with histidine-rich heavy-metal-associated sequences. 
  • Nucleotide interactions with the dicyclohexylcarbodiimide-sensitive adenosinetriphosphatase from spinach chloroplasts. 
  • PINA is essential for growth and positively influences NIMA function in Aspergillus nidulans. 
  • PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. 
  • Peptidylglycine α-amidating monooxygenase heterozygosity alters brain copper handling with region specificity. 
  • Phorbol esters induce differentiation in a pre-B-lymphocyte cell line by enhancing Na+/H+ exchange. 
  • Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation. 
  • Pleiotropic plasma membrane ATPase mutations of Saccharomyces cerevisiae. 
  • RANBP2 is an allosteric activator of the conventional kinesin-1 motor protein, KIF5B, in a minimal cell-free system. 
  • Recognition and repair of compound DNA lesions (base damage and mismatch) by human mismatch repair and excision repair systems. 
  • Reduced expression of mismatch repair genes in colorectal cancer patients in Egypt. 
  • Reduced expression of mismatch repair genes measured by multiplex reverse transcription-polymerase chain reaction in human gliomas. 
  • Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains. 
  • Regulation of transcription of the repA1 gene in the replication control region of IncFII plasmid NR1 by gene dosage of the repA2 transcription repressor protein. 
  • Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs. 
  • Reversible inhibition of Hsp70 chaperone function by Scythe and Reaper. 
  • Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. 
  • Role of conserved residues within the carboxy phosphate domain of carbamoyl phosphate synthetase. 
  • Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. 
  • Roles of RAD6 epistasis group members in spontaneous polzeta-dependent translesion synthesis in Saccharomyces cerevisiae. 
  • Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd. 
  • Saccharomyces cerevisiae MutLalpha is a mismatch repair endonuclease. 
  • Saccharomyces cerevisiae RAD5-encoded DNA repair protein contains DNA helicase and zinc-binding sequence motifs and affects the stability of simple repetitive sequences in the genome. 
  • Segrosome structure revealed by a complex of ParR with centromere DNA. 
  • Separation of meiotic and mitotic effects of claret non-disjunctional on chromosome segregation in Drosophila. 
  • Sex-induced silencing defends the genome of Cryptococcus neoformans via RNAi. 
  • Significant effect of homologous recombination DNA repair gene polymorphisms on pancreatic cancer survival. 
  • Single nucleotide polymorphisms of RecQ1, RAD54L, and ATM genes are associated with reduced survival of pancreatic cancer. 
  • Sliding clamp of the bacteriophage T4 polymerase has open and closed subunit interfaces in solution. 
  • SnRK1A-mediated phosphorylation of a cytosolic ATPase positively regulates rice innate immunity and is inhibited by Ustilaginoidea virens effector SCRE1. 
  • Somatic mutation of hPMS2 as a possible cause of sporadic human colon cancer with microsatellite instability. 
  • Spatial relationships between specific sites on reconstituted chloroplast proton adenosinetriphosphatase and the phospholipid vesicle surface. 
  • Steady state kinetics of soluble and membrane-bound mitochondrial ATPase. 
  • Steady-state ATP synthesis by bacteriorhodopsin and chloroplast coupling factor co-reconstituted into asolectin vesicles. 
  • Structural and functional domains of the Drosophila ncd microtubule motor protein. 
  • Structural investigation of reconstituted chloroplast ATPase with fluorescence measurements. 
  • Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation. 
  • Structure and function of the UvrB protein. 
  • Structure of the endonuclease domain of MutL: unlicensed to cut. 
  • Structures of maintenance of carboxysome distribution Walker-box McdA and McdB adaptor homologs. 
  • Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation. 
  • Structures of the T. brucei kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold. 
  • Subcellular localization of spastin: implications for the pathogenesis of hereditary spastic paraplegia. 
  • Techniques for the study of the biochemical basis of drug action. 
  • The DEAH-box RNA helicase RHAU binds an intramolecular RNA G-quadruplex in TERC and associates with telomerase holoenzyme. 
  • The DNA helicase activities of Rad3 protein of Saccharomyces cerevisiae and helicase II of Escherichia coli are differentially inhibited by covalent and noncovalent DNA modifications. 
  • The Drosophila claret segregation protein is a minus-end directed motor molecule. 
  • The MutL ATPase is required for mismatch repair. 
  • The bacterial effector GarD shields Chlamydia trachomatis inclusions from RNF213-mediated ubiquitylation and destruction. 
  • The beta sliding clamp binds to multiple sites within MutL and MutS. 
  • The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. 
  • The candidate sour taste receptor, PKD2L1, is expressed by type III taste cells in the mouse. 
  • The coiled coils of cohesin are conserved in animals, but not in yeast. 
  • The emerging kinesin family of microtubule motor proteins. 
  • The filamentous bacteriophage assembly proteins require the bacterial SecA protein for correct localization to the membrane. 
  • The mismatch DNA repair heterodimer, hMSH2/6, regulates BLM helicase. 
  • The role of calmodulin in the structure and regulation of phosphorylase kinase from rabbit skeletal muscle. 
  • Thyroxine and propylthiouracil effects on alpha- and beta-adrenergic receptor number, ATPase activities, and sialic acid content of rat cardiac membrane vesicles. 
  • Transmembrane signals generated through MHC class II, CD19, CD20, CD39, and CD40 antigens induce LFA-1-dependent and independent adhesion in human B cells through a tyrosine kinase-dependent pathway. 
  • Uncoupling of nucleotide hydrolysis and polymerization in the ParA protein superfamily disrupts DNA segregation dynamics. 
  • VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. 
  • VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD. 
  • Viral packaging ATPases utilize a glutamate switch to couple ATPase activity and DNA translocation. 
  • Virus-infected avian cell lines established in vitro. 
  • WLS-dependent secretion of WNT3A requires Ser209 acylation and vacuolar acidification. 
  • hMutSalpha- and hMutLalpha-dependent phosphorylation of p53 in response to DNA methylator damage. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • McLendon, Roger Edwin, Professor of Pathology, Pathology