Affinity Labels

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  Subject Areas on Research

  • 125I-labeled p-azidobenzylcarazolol, a photoaffinity label for the beta-adrenergic receptor. Characterization of the ligand and photoaffinity labeling of beta 1- and beta 2-adrenergic receptors. 
  • Agonist-dependent phosphorylation of the alpha 2-adrenergic receptor by the beta-adrenergic receptor kinase. 
  • Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1. 
  • Beta-adrenergic regulation of cholecystokinin secretion in STC-1 cells. 
  • Biochemical characterization of the adrenergic receptors: affinity labeling, purification, and reconstitution studies. 
  • Catecholamine-induced desensitization of turkey erythrocyte adenylate cyclase. Structural alterations in the beta-adrenergic receptor revealed by photoaffinity labeling. 
  • Characterization of cardiac A1 adenosine receptors by ligand binding and photoaffinity labeling. 
  • Characterization of guanosine diphospho-D-mannose dehydrogenase from Pseudomonas aeruginosa. Structural analysis by limited proteolysis. 
  • Cofractionation of the 17-kD PK 14105 binding site protein with solubilized peripheral-type benzodiazepine binding sites. 
  • Covalent labeling of the beta-adrenergic ligand-binding site with para-(bromoacetamidyl)benzylcarazolol. A highly potent beta-adrenergic affinity label. 
  • Covalent labeling of the cerebral cortex alpha 1-adrenergic receptor with a new high affinity radioiodinated photoaffinity probe. 
  • Covalent labelling of ligand binding sites of human placental S-adenosylhomocysteine hydrolase with 8-azido derivatives of adenosine and cyclic AMP. 
  • Definition of cytochrome c binding domains by chemical modification. Interaction of horse cytochrome c with beef sulfite oxidase and analysis of steady state kinetics. 
  • Development of an affinity ligand for purification of alpha 2-adrenoceptors from human platelet membranes. 
  • Development of novel tumor imaging agents with phage-display combinatorial peptide libraries. 
  • Fast ion-exchange membrane purification of a microsomal protein. 
  • Identification of the D2-dopamine receptor binding subunit in several mammalian tissues and species by photoaffinity labeling. 
  • Identification of the binding subunit of the D1-dopamine receptor by photoaffinity crosslinking. 
  • Identification of the ligand-binding subunit of the human 5-hydroxytryptamine1A receptor with N-(p-azido-m-[125I] iodophenethyl)spiperone, a high affinity radioiodinated photoaffinity probe. 
  • Identification of the subunit structure of rat pineal adrenergic receptors by photoaffinity labeling. 
  • Improved targeting of an anti-epidermal growth factor receptor variant III monoclonal antibody in tumor xenografts after labeling using N-succinimidyl 5-iodo-3-pyridinecarboxylate. 
  • Inhibitors of protein disulfide isomerase suppress apoptosis induced by misfolded proteins. 
  • Interaction of photoreactive and fluorescent nucleotides with chloroplast coupling factor 1. 
  • Investigation of nucleotide binding sites on chloroplast coupling factor 1 with 3'O-(4-benzoyl)benzoyl adenosine 5'-triphosphate. 
  • Isolation and characterization of the beta-adrenergic receptor of frog erythrocytes and development of potential affinity ligands. 
  • Localization of the fourth membrane spanning domain as a ligand binding site in the human platelet alpha 2-adrenergic receptor. 
  • Mammalian beta-adrenergic receptors. Distinct glycoprotein populations containing high mannose or complex type carbohydrate chains. 
  • Mammalian beta-adrenergic receptors. Structural differences in beta 1 and beta 2 subtypes revealed by peptide maps. 
  • Mechanisms of membrane-receptor regulation. Biochemical, physiological, and clinical insights derived from studies of the adrenergic receptors. 
  • Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway. 
  • Molecular biology of adrenergic receptors in the rat and frog central nervous system. 
  • Molecular characterization of dopamine receptors. 
  • Molecular characterization of the beta-adrenergic receptor of frog erythrocytes. 
  • Phorbol diesters promote beta-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes. 
  • Phosphorylation of the beta-adrenergic receptor accompanies catecholamine-induced desensitization of turkey erythrocyte adenylate cyclase. 
  • Photoaffinity cross-linking of a radioiodinated probe, 125I-A55453, into alpha 1-adrenergic receptors. 
  • Photoaffinity labeling of beta-adrenergic receptors in mammalian tissues. 
  • Photoaffinity labeling of mammalian alpha 1-adrenergic receptors. Identification of the ligand binding subunit with a high affinity radioiodinated probe. 
  • Photoaffinity labeling of the D2-dopamine receptor using a novel high affinity radioiodinated probe. 
  • Photoaffinity labeling of the beta-adrenergic receptor. 
  • Photoaffinity labeling of turkey erythrocyte beta-adrenergic receptors: degradation of the Mr = 49,000 protein explains apparent heterogeneity. 
  • Photoaffinity labelling of mammalian beta-adrenergic receptors: metal-dependent proteolysis explains apparent heterogeneity. 
  • Photochemical control of endogenous ion channels and cellular excitability. 
  • Purification and characterization of a protein associated with peripheral-type benzodiazepine binding sites. 
  • Purification and characterization of the D2-dopamine receptor from bovine anterior pituitary. 
  • Solubilization and characterization of D2-dopamine receptors in an estrone-induced, prolactin-secreting rat pituitary adenoma. 
  • Specific photoaffinity labelling of inhibitory adenosine receptors. 
  • Structure and function of beta-adrenergic receptors: regulation at the molecular level. 
  • Subtype-specific binding of azidoanilido-GTP by purified G protein alpha subunits. 
  • Suicide substrates for the alanine racemase of Escherichia coli B. 
  • Synthesis of iodine-125 labeled (+/-)-15-(4-azidobenzyl)carazolol: a potent beta-adrenergic photoaffinity probe. 
  • The ATP binding site of the yeast plasma membrane proton-translocating ATPase. 
  • The beta 1-adrenergic receptor of the turkey erythrocyte. Molecular heterogeneity revealed by purification and photoaffinity labeling. 
  • The beta-adrenergic receptor: rapid purification and covalent labeling by photoaffinity crosslinking. 
  • The cardiac beta-adrenergic receptor. Structural similarities of beta 1 and beta 2 receptor subtypes demonstrated by photoaffinity labeling. 
  • The rat testicular A1 adenosine receptor-adenylate cyclase system. 
  • The sublaminar organization of corticogeniculate neurons in layer 6 of macaque striate cortex. 
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  Keywords of People

  • Broverman, Sherryl A., Associate Professor of the Practice of Biology, Biology
  • Patz Jr., Edward F., James and Alice Chen Professor of Radiology, Pathology