Affinity Labels
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Subject Areas on Research
- 125I-labeled p-azidobenzylcarazolol, a photoaffinity label for the beta-adrenergic receptor. Characterization of the ligand and photoaffinity labeling of beta 1- and beta 2-adrenergic receptors.
- Agonist-dependent phosphorylation of the alpha 2-adrenergic receptor by the beta-adrenergic receptor kinase.
- Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1.
- Beta-adrenergic regulation of cholecystokinin secretion in STC-1 cells.
- Biochemical characterization of the adrenergic receptors: affinity labeling, purification, and reconstitution studies.
- Catecholamine-induced desensitization of turkey erythrocyte adenylate cyclase. Structural alterations in the beta-adrenergic receptor revealed by photoaffinity labeling.
- Characterization of UDP-glucuronic acid transport in rat liver microsomal vesicles with photoaffinity analogs.
- Characterization of cardiac A1 adenosine receptors by ligand binding and photoaffinity labeling.
- Cofractionation of the 17-kD PK 14105 binding site protein with solubilized peripheral-type benzodiazepine binding sites.
- Covalent labeling of the beta-adrenergic ligand-binding site with para-(bromoacetamidyl)benzylcarazolol. A highly potent beta-adrenergic affinity label.
- Covalent labeling of the cerebral cortex alpha 1-adrenergic receptor with a new high affinity radioiodinated photoaffinity probe.
- Covalent labelling of ligand binding sites of human placental S-adenosylhomocysteine hydrolase with 8-azido derivatives of adenosine and cyclic AMP.
- Current trends in differential expression proteomics: isotopically coded tags.
- Definition of cytochrome c binding domains by chemical modification. Interaction of horse cytochrome c with beef sulfite oxidase and analysis of steady state kinetics.
- Development of an affinity ligand for purification of alpha 2-adrenoceptors from human platelet membranes.
- Development of novel tumor imaging agents with phage-display combinatorial peptide libraries.
- Endogenous proteinases modulate the function of the beta-adrenergic receptor-adenylate cyclase system.
- Evidence against posttranslational glycosylation of rat glucocorticoid receptors.
- Fast ion-exchange membrane purification of a microsomal protein.
- Identification of the ligand-binding subunit of the human 5-hydroxytryptamine1A receptor with N-(p-azido-m-[125I] iodophenethyl)spiperone, a high affinity radioiodinated photoaffinity probe.
- Identification of the subunit structure of rat pineal adrenergic receptors by photoaffinity labeling.
- Improved targeting of an anti-epidermal growth factor receptor variant III monoclonal antibody in tumor xenografts after labeling using N-succinimidyl 5-iodo-3-pyridinecarboxylate.
- Interaction of photoreactive and fluorescent nucleotides with chloroplast coupling factor 1.
- Investigation of nucleotide binding sites on chloroplast coupling factor 1 with 3'O-(4-benzoyl)benzoyl adenosine 5'-triphosphate.
- Isolation and characterization of the beta-adrenergic receptor of frog erythrocytes and development of potential affinity ligands.
- Localization of cortical granule constituents before and after exocytosis in the hamster egg.
- Localization of the fourth membrane spanning domain as a ligand binding site in the human platelet alpha 2-adrenergic receptor.
- Mammalian beta-adrenergic receptors. Distinct glycoprotein populations containing high mannose or complex type carbohydrate chains.
- Mammalian beta-adrenergic receptors. Structural differences in beta 1 and beta 2 subtypes revealed by peptide maps.
- Mechanisms of membrane-receptor regulation. Biochemical, physiological, and clinical insights derived from studies of the adrenergic receptors.
- Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway.
- Molecular biology of adrenergic receptors in the rat and frog central nervous system.
- Molecular characterization of the beta-adrenergic receptor of frog erythrocytes.
- Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis.
- Phorbol diesters promote beta-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes.
- Phosphorylation of the beta-adrenergic receptor accompanies catecholamine-induced desensitization of turkey erythrocyte adenylate cyclase.
- Photoaffinity cross-linking of a radioiodinated probe, 125I-A55453, into alpha 1-adrenergic receptors.
- Photoaffinity labeling of beta-adrenergic receptors in mammalian tissues.
- Photoaffinity labeling of human platelet and rabbit kidney alpha 2-adrenoceptors with [3H]SKF 102229.
- Photoaffinity labeling of mammalian alpha 1-adrenergic receptors. Identification of the ligand binding subunit with a high affinity radioiodinated probe.
- Photoaffinity labeling of the beta-adrenergic receptor.
- Photoaffinity labeling of turkey erythrocyte beta-adrenergic receptors: degradation of the Mr = 49,000 protein explains apparent heterogeneity.
- Photoaffinity labelling of mammalian beta-adrenergic receptors: metal-dependent proteolysis explains apparent heterogeneity.
- Photochemical control of endogenous ion channels and cellular excitability.
- Plasma membrane receptors.
- Purification and characterization of a protein associated with peripheral-type benzodiazepine binding sites.
- Ribotrap : targeted purification of RNA-specific RNPs from cell lysates through immunoaffinity precipitation to identify regulatory proteins and RNAs.
- Specific photoaffinity labelling of inhibitory adenosine receptors.
- Structure and function of beta-adrenergic receptors: regulation at the molecular level.
- Subtype-specific binding of azidoanilido-GTP by purified G protein alpha subunits.
- Suicide substrates for the alanine racemase of Escherichia coli B.
- Synthesis of iodine-125 labeled (+/-)-15-(4-azidobenzyl)carazolol: a potent beta-adrenergic photoaffinity probe.
- The ATP binding site of the yeast plasma membrane proton-translocating ATPase.
- The beta 1-adrenergic receptor of the turkey erythrocyte. Molecular heterogeneity revealed by purification and photoaffinity labeling.
- The beta-adrenergic receptor: rapid purification and covalent labeling by photoaffinity crosslinking.
- The cardiac beta-adrenergic receptor. Structural similarities of beta 1 and beta 2 receptor subtypes demonstrated by photoaffinity labeling.
- The rat testicular A1 adenosine receptor-adenylate cyclase system.
- The sublaminar organization of corticogeniculate neurons in layer 6 of macaque striate cortex.
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Keywords of People
- Patz Jr., Edward F., James and Alice Chen Distinguished Professor of Radiology, Pathology