Bacteriological Techniques

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  Subject Areas on Research

  • A randomized controlled trial of standard versus intensified tuberculosis diagnostics on treatment decisions by physicians in Northern Tanzania. 
  • A single-tube screen for Salmonella and Shigella. 
  • An innovative pay-for-performance (P4P) strategy for improving malaria management in rural Kenya: protocol for a cluster randomized controlled trial. 
  • Analysis of enteric coagulase-negative staphylococci from neonates with necrotizing enterocolitis. 
  • Analysis of total aerobic viable counts in raw fish by high-throughput optical oxygen respirometry. 
  • Antibiotic susceptibility testing of Pseudomonas aeruginosa: selection of a control strain and criteria for magnesium and calcium content in media. 
  • Assessment of a quantitative multiplex 5' nuclease real-time PCR for spotted fever and typhus group rickettsioses and Orientia tsutsugamushi. 
  • Automated blood culture systems. 
  • Bacterial gastroenteritis. 
  • Bartonella species in blood of immunocompetent persons with animal and arthropod contact. 
  • Bioluminescence screening for bacteriuria. 
  • Blood culture contamination in Tanzania, Malawi, and the United States: a microbiological tale of three cities. 
  • Blood culture contamination with Enterococci and skin organisms: implications for surveillance definitions of primary bloodstream infections. 
  • Chorioamnionitis and Culture-Confirmed, Early-Onset Neonatal Infections. 
  • Clinical importance of identifying coagulase-negative staphylococci isolated from blood cultures: evaluation of MicroScan Rapid and Dried Overnight Gram-Positive panels versus a conventional reference method. 
  • Clinical research and development of tuberculosis diagnostics: moving from silos to synergy. 
  • Clinical utility of broth cultures of cerebrospinal fluid from patients at risk for shunt infections. 
  • Colicin typing as an epidemiological tool in the investigation of outbreaks of Shigella sonnei. 
  • Community care of corneal ulcers. 
  • Comparing Standard Versus Extended Culture Duration in Acute Hip and Knee Periprosthetic Joint Infection. 
  • Comparison of CampyPak II with standard 5% oxygen and candle jars for growth of Campylobacter jejuni from human feces. 
  • Comparison of PCR, culture, and histopathology for diagnosis of tuberculous pericarditis. 
  • Comparison of atmospheres of incubation for primary isolation of Campylobacter fetus subsp. jejuni from animal specimens: 5% oxygen versus candle jar. 
  • Comparison of two rapid assays for Clostridium difficile Common antigen and a C difficile toxin A/B assay with the cell culture neutralization assay. 
  • Controlled clinical comparison of BACTEC plus anaerobic/F to standard anaerobic/F as the anaerobic companion bottle to plus aerobic/F medium for culturing blood from adults. 
  • Controlled clinical comparison of BacT/ALERT standard aerobic and standard anaerobic blood culture bottles inoculated directly or after transport in sodium polyanethol sulfonate tubes. 
  • Controlled clinical comparison of BacT/ALERT standard aerobic medium with BACTEC standard aerobic medium for culturing blood. 
  • Controlled clinical comparison of BacT/alert FA plus and FN plus blood culture media with BacT/alert FA and FN blood culture media. 
  • Controlled clinical comparison of VersaTREK and BacT/ALERT blood culture systems. 
  • Controlled clinical comparison of bioMérieux VITAL and BACTEC NR-660 blood culture systems for detection of bacteremia and fungemia in adults. 
  • Controlled clinical comparison of plastic versus glass bottles of BacT/ALERT PF medium for culturing blood from children. 
  • Controlled comparison of BACTEC 13A, MYCO/F LYTIC, BacT/ALERT MB, and ISOLATOR 10 systems for detection of mycobacteremia. 
  • Controlled comparison of BacT/Alert MB system, manual Myco/F lytic procedure, and isolator 10 system for diagnosis of Mycobacterium tuberculosis Bacteremia. 
  • Controlled comparison of a new Becton Dickinson agar slant blood culture system with Roche Septi-Chek for the detection of bacteremia and fungemia. 
  • Controlled comparison of the BacT/Alert and BACTEC 660/730 nonradiometric blood culture systems. 
  • Controlled evaluation of 5 versus 10 milliliters of blood cultured in aerobic BacT/Alert blood culture bottles. 
  • Controlled evaluation of BACTEC Plus 26 and Roche Septi-Chek aerobic blood culture bottles. 
  • Controlled evaluation of BacT/Alert standard aerobic and FAN aerobic blood culture bottles for detection of bacteremia and fungemia. 
  • Controlled evaluation of Bactec Peds Plus/F and Bactec lytic/10 anaerobic/F media for isolation of Salmonella enterica serovars typhi and paratyphi A from blood. 
  • Controlled multicenter evaluation of a bacteriophage-based method for rapid detection of Staphylococcus aureus in positive blood cultures. 
  • Coproexamination for botulinal toxin and clostridium botulinum. A new procedure for laboratory diagnosis of botulism. 
  • Correlation between sinus and lung cultures in lung transplant patients with cystic fibrosis. 
  • Correlation of serum bactericidal activity with antimicrobial agent level and minimal bactericidal concentration. 
  • Creatinine measurement: state of the art in accuracy and interlaboratory harmonization. 
  • Cyanoacrylate dressings: are they microbiologically impermeable? 
  • Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis. 
  • Detection of Clostridium difficile as a routine diagnosis: comparison of real-time PCR and enzyme immunoassay. 
  • Detection of bacterial contamination in apheresis platelets: is apheresis technology a factor? 
  • Diagnosis of streptococcal pharyngitis by detection of Streptococcus pyogenes in posterior pharyngeal versus oral cavity specimens. 
  • Different methods of sample preparation influence sensitivity of Mycobacterium tuberculosis and Borrelia burgdorferi PCR. 
  • Disinfectant testing using a modified use-dilution method: collaborative study. 
  • Does nonpayment for hospital-acquired catheter-associated urinary tract infections lead to overtesting and increased antimicrobial prescribing? 
  • Does preoperative topical antimicrobial scrub reduce positive surgical site culture rates in men undergoing artificial urinary sphincter placement? 
  • Effect of agitation and terminal subcultures on yield and speed of detection of the Oxoid Signal blood culture system versus the BACTEC radiometric system. 
  • Effect of altered headspace atmosphere on yield and speed of detection of the Oxoid Signal blood culture system versus the BACTEC radiometric system. 
  • Effects of storage in an anaerobic transport system on bacteria in known polymicrobial mixtures and in clinical specimens. 
  • Enhanced culture detection of Citrobacter koseri from cerebrospinal fluid in BacTec. 
  • Evaluation of transport and storage techniques for isolation of Campylobacter fetus subsp. jejuni from turkey cecal specimens. 
  • Evaluation of transport media for Campylobacter jejuni in human fecal specimens. 
  • False-positive Gram-stained smears. 
  • General guidelines for clinical bacteriology. Infectious Diseases Society of America and the Food and Drug Administration. 
  • Immunochromatographic test for rapid detection of Streptococcus pneumoniae in the nasopharynx. 
  • Impact of multiple policy interventions on the screening and diagnosis of drug-resistant tuberculosis patients: a cascade analysis on six prefectures in China. 
  • Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. 
  • In vitro phagocytosis and intracellular fate of variously encapsulated strains of Cryptococcus neoformans. 
  • Interpretive criteria and quality control for antimicrobial susceptibility tests of levofloxacin. 
  • Intravenous amoxicillin/clavulanate for the prevention of bacteraemia following dental procedures: a randomized clinical trial. 
  • Issues in cerebrospinal fluid management. Acid-fast bacillus smear and culture. 
  • Laboratory and clinical evaluation of Mycobacterium xenopi isolates. 
  • Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli. 
  • Long-term monitoring of bacteria undergoing programmed population control in a microchemostat. 
  • MRSA nares swab is a more accurate predictor of MRSA wound infection compared with clinical risk factors in emergency department patients with skin and soft tissue infections. 
  • Mating pair formation homologue TraG is a variable membrane protein essential for contact-independent type IV secretion of chromosomal DNA by Neisseria gonorrhoeae. 
  • Medium matters: modeling the impact of solid medium performance on tuberculosis trial sample size requirements. 
  • Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens. 
  • Multicenter collaborative evaluation of a standardized serum bactericidal test as a prognostic indicator in infective endocarditis. 
  • Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs. 
  • Multiple fluid collections: CT- or US-guided aspiration--evaluation of microbiologic results and implications for clinical practice. 
  • Next-generation sequencing not superior to culture in periprosthetic joint infection diagnosis. 
  • Optimizing Zn porphyrin-based photosensitizers for efficient antibacterial photodynamic therapy. 
  • PCR identification of Mycobacterium bovis BCG. 
  • Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia. 
  • Performance of rapid streptococcal antigen testing varies by personnel. 
  • Performance of the cobas MRSA/SA Test for Simultaneous Detection of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus From Nasal Swabs. 
  • Porphyromonas gingivalis-mediated Epithelial Cell Entry of HIV-1. 
  • Predicting urine culture results by dipstick testing and phase contrast microscopy. 
  • Pseudomonas aeruginosa serology and risk for re-isolation in the EPIC trial. 
  • Recovery of clinically important microorganisms from the BacT/Alert blood culture system does not require testing for seven days. 
  • Recurrent pseudobacteremias traced to a radiometric blood culture device. 
  • Rejection criteria for endotracheal aspirates from adults. 
  • Relevance of the number of positive bottles in determining clinical significance of coagulase-negative staphylococci in blood cultures. 
  • Remarks concerning testing parameters for blood cultures. 
  • Repeat lumbar punctures in infants with meningitis in the neonatal intensive care unit. 
  • Revival of Seeliger's historical 'Special Listeria Culture Collection'. 
  • Rhipicephalus sanguineus: vector of a new spotted fever group rickettsia in the United States. 
  • STUDIES OF THE STREPTOCOCCUS AGALACTIAE FORM OF MASTITIS IN DAIRY CATTLE. 
  • Sampling out: regulatory avoidance and the Total Coliform Rule. 
  • Serum dilution test for bactericidal activity. I. Selection of a physiologic diluent. 
  • Serum dilution test for bactericidal activity. II. Standardization and correlation with antimicrobial assays and susceptibility tests. 
  • Standardization of bacterial numbers of penicylinders used in disinfectant testing: interlaboratory study. 
  • Staphylococcus aureus bacteremia after median sternotomy: clinical utility of blood culture results in the identification of postoperative mediastinitis. 
  • Streptococcus pneumoniae antigen test using positive blood culture bottles as an alternative method to diagnose pneumococcal bacteremia. 
  • Subcultures of BACTEC-positive but gram or acridine orange stain-negative NR 6A and 7A blood culture bottles are unnecessary. 
  • Survival of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus spp. for an extended period of transport. 
  • The Role of Stewardship in Addressing Antibacterial Resistance: Stewardship and Infection Control Committee of the Antibacterial Resistance Leadership Group. 
  • The easiest children to reach are most likely to be infected with ocular Chlamydia trachomatis in trachoma endemic areas of Niger. 
  • The importance of a multifaceted approach to characterizing the microbial flora of chronic wounds. 
  • The role of smears, cultures, and antibiotic sensitivity testing in the management of suspected infectious keratitis. 
  • Two decades of disseminated tuberculosis at a university medical center: the expanding role of mycobacterial blood culture. 
  • Understanding the costs of better tuberculosis diagnostics. 
  • Unsuspected leptospirosis is a cause of acute febrile illness in Nicaragua. 
  • Use of a sodium polyanetholesulfonate disk for the identification of Gardnerella vaginalis. 
  • Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea. 
  • Utility of extended blood culture incubation for isolation of Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella organisms: a retrospective multicenter evaluation. 
  • Utility of obtaining blood cultures in febrile neutropenic patients undergoing bone marrow transplantation. 
  • Validation of performance of plastic versus glass bottles for culturing anaerobes from blood in BacT/ALERT SN medium. 
  • Value of routine anaerobic blood cultures for pediatric patients. 
  • Vitrectomy for the diagnosis and management of uveitis of unknown cause. 
  • WHO guidelines for antimicrobial treatment in children admitted to hospital in an area of intense Plasmodium falciparum transmission: prospective study. 
  • Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile.