beta-Galactosidase

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  Subject Areas on Research

  • 2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor. 
  • 5' exon replacement and repair by spliceosome-mediated RNA trans-splicing. 
  • A cAMP response element in the beta 2-adrenergic receptor gene confers transcriptional autoregulation by cAMP. 
  • A calorimetric and equilibrium investigation of the hydrolysis of lactose. 
  • A common mutation site in the beta-galactosidase gene originates in Puerto Rico. 
  • A new helper phospholipid for gene delivery. 
  • A pressure-mediated nonviral method for efficient arterial gene and oligonucleotide transfer. 
  • AMH induces mesonephric cell migration in XX gonads. 
  • Activation of MEF2 by muscle activity is mediated through a calcineurin-dependent pathway. 
  • Activation of the Saccharomyces cerevisiae heat shock transcription factor under glucose starvation conditions by Snf1 protein kinase. 
  • Adenoviral reporter gene transfer to the human trabecular meshwork does not alter aqueous humor outflow. Relevance for potential gene therapy of glaucoma. 
  • Adenoviral-mediated gene transfer induces sustained pericardial VEGF expression in dogs: effect on myocardial angiogenesis. 
  • Adenovirus-mediated gene transfer into rat cardiac allografts. Comparison of direct injection and perfusion. 
  • Adenovirus-mediated gene transfer to nonparenchymal cells in normal and injured liver. 
  • Adenovirus-mediated genetic manipulation of the myocardial beta-adrenergic signaling system in transplanted hearts. 
  • Adenovirus-mediated transfer of the CFTR gene to lung of nonhuman primates: toxicity study. 
  • Analysis of muscle creatine kinase regulatory elements in recombinant adenoviral vectors. 
  • Antigen-specific induction of peripheral T cell tolerance in vivo by codelivery of DNA vectors encoding antigen and Fas ligand. 
  • Antigenicity of a recombinant Ro (SS-A) fusion protein. 
  • Appearance of protease activities coincides with p10 and polyhedrin-driven protein production in the baculovirus expression system: effects on yield. 
  • Aromatic hydrocarbon receptor polymorphism: development of new methods to correlate genotype with phenotype. 
  • Aryl hydrocarbon receptor activation in genital tubercle, palate, and other embryonic tissues in 2,3,7, 8-tetrachlorodibenzo-p-dioxin-responsive lacZ mice. 
  • Atomic Resolution Cryo-EM Structure of β-Galactosidase. 
  • Autoregulatory control of E2F1 expression in response to positive and negative regulators of cell cycle progression. 
  • Axonal plasticity elicits long-term changes in oligodendroglia and myelinated fibers. 
  • Bioelectronic measurement and feedback control of molecules in living cells. 
  • Bleomycin induces the hsp 70 heat shock promoter in cultured cells. 
  • Carbohydrate on human factor VIII/von Willebrand factor. Impairment of function by removal of specific galactose residues. 
  • Cardiac gene delivery with cardiopulmonary bypass. 
  • Cardiac-specific LIM protein FHL2 modifies the hypertrophic response to beta-adrenergic stimulation. 
  • Catheter-based intracoronary myocardial adenoviral gene delivery: importance of intraluminal seal and infusion flow rate. 
  • Catheter-mediated subselective intracoronary gene delivery to the rabbit heart: introduction of a novel method. 
  • Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus. 
  • Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy. 
  • Cellular senescence in the glaucomatous outflow pathway. 
  • Charge-reversal amphiphiles for gene delivery. 
  • Classification and genetic characterization of pattern-forming Bacilli. 
  • Cloning and characterization of the major promoter of the human protein kinase C beta gene. Regulation by phorbol esters. 
  • Conditional immortalization of neuronal cells from postmitotic cultures and adult CNS. 
  • Controlled gene delivery by DNA-gelatin nanospheres. 
  • Convenient vectors for cloning and sequencing EcoRI and HindIII fragments. 
  • Cti6 is an Rpd3-Sin3 histone deacetylase-associated protein required for growth under iron-limiting conditions in Saccharomyces cerevisiae. 
  • Cyclophosphamide diminishes inflammation and prolongs transgene expression following delivery of adenoviral vectors to mouse liver and lung. 
  • Cytosine deamination in mismatched base pairs. 
  • Delivery of primary autologous skeletal myoblasts into rabbit heart by coronary infusion: a potential approach to myocardial repair. 
  • Desert Hedgehog/Patched 1 signaling specifies fetal Leydig cell fate in testis organogenesis. 
  • Differential expression of the ARF GAP genes GIT1 and GIT2 in mouse tissues. 
  • Differential regulation of dopamine D1A receptor responsiveness by various G protein-coupled receptor kinases. 
  • Distinct mesodermal signals, including BMPs from the septum transversum mesenchyme, are required in combination for hepatogenesis from the endoderm. 
  • Distinctive patterns of translational reinitiation in the lac repressor mRNA: bridging of long distances by out-of-frame translation and RNA secondary structure, effects of primary sequence. 
  • Does thalidomide affect IL-2 response and production? 
  • Dynamic patterns of retinoic acid synthesis and response in the developing mammalian heart. 
  • Effect of NF-kappa B inhibition on TNF-alpha-induced apoptosis in human RPE cells. 
  • Effect of a dominant negative ras on myocardial hypertrophy by using adenoviral-mediated gene transfer. 
  • Effect of isopropyl-beta-D-thiogalactopyranosid induction of the lac operon on the specificity of spontaneous and doxorubicin-induced mutations in Escherichia coli. 
  • Effect of mutant IkappaB on cytokine-induced activation of NF-kappaB in cultured human RPE cells. 
  • Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes. 
  • Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model. 
  • Efficient adenoviral gene transfer to early venous bypass grafts: comparison with native vessels. 
  • Efficient in vivo gene transfer into the heart in the rat myocardial infarction model using the HVJ (Hemagglutinating Virus of Japan)--liposome method. 
  • Endothelial Bmp4 is induced during arterial remodeling: effects on smooth muscle cell migration and proliferation. 
  • Enhanced gene expression in mouse muscle by sustained release of plasmid DNA using PPE-EA as a carrier. 
  • Enhanced growth of small bowel in transgenic mice overexpressing bovine growth hormone. 
  • Enhanced vasorelaxation by overexpression of beta 2-adrenergic receptors in large arteries. 
  • Enhancer blocking by the Drosophila gypsy insulator depends upon insulator anatomy and enhancer strength. 
  • Eradication of solid human breast tumors in nude mice with an intravenously injected light-emitting oncolytic vaccinia virus. 
  • Estrogen levels in childhood determined by an ultrasensitive recombinant cell bioassay. 
  • Ex vivo adenovirus-mediated gene transfer to the adult rat heart. 
  • Experimental and numerical studies of adenovirus delivery to outflow tissues of perfused human anterior segments. 
  • Expression analysis of the matrix GLA protein and VE-cadherin gene promoters in the outflow pathway. 
  • Expression of a peptide inhibitor of protein phosphatase 1 increases phosphorylation and activity of CREB in NIH 3T3 fibroblasts. 
  • Expression of the 71 kDa dystrophin isoform (Dp71) evaluated by gene targeting. 
  • Expression of transcription factor E2F1 induces quiescent cells to enter S phase. 
  • Fetal gene transfer by transuterine injection of cationic liposome-DNA complexes. 
  • Fusigenic liposome-mediated DNA transfer into cardiac myocytes. 
  • GRP94-associated enzymatic activities. Resolution by chromatographic fractionation. 
  • Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits. 
  • Gene transfer to the human trabecular meshwork by anterior segment perfusion. 
  • Genetic evidence that the Tat proteins of human immunodeficiency virus types 1 and 2 can multimerize in the eukaryotic cell nucleus. 
  • Genomic analysis of sequence variation in tandemly repeated DNA. Evidence for localized homogeneous sequence domains within arrays of alpha-satellite DNA. 
  • Gli activity correlates with tumor grade in platelet-derived growth factor-induced gliomas. 
  • Glomerular beta-galactosidase expression following transduction with microsphere-adenoviral complexes. 
  • Growth factors regulate beta-catenin-mediated TCF-dependent transcriptional activation in fibroblasts during the proliferative phase of wound healing. 
  • Hepatocyte telomere shortening and senescence are general markers of human liver cirrhosis. 
  • High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae. 
  • Hmo1p, a high mobility group 1/2 homolog, genetically and physically interacts with the yeast FKBP12 prolyl isomerase. 
  • Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery. 
  • Identification of a negative regulatory function for steroid receptors. 
  • Identifying a transcription factor interaction site on RNA polymerase II. 
  • Immune responses to viral antigens versus transgene product in the elimination of recombinant adenovirus-infected hepatocytes in vivo. 
  • Immunology of gene therapy with adenoviral vectors in mouse skeletal muscle. 
  • In vivo cytotoxic T lymphocyte induction with soluble proteins administered in liposomes. 
  • Inhibition of Bmp signaling affects growth and differentiation in the anagen hair follicle. 
  • Interactions of estrogen- and thyroid hormone receptors on a progesterone receptor estrogen response element (ERE) sequence: a comparison with the vitellogenin A2 consensus ERE. 
  • Intercellular relationships in the synthesis of macromolecules by organ cultures of corneas. 
  • Intracoronary adenovirus-mediated delivery and overexpression of the beta(2)-adrenergic receptor in the heart : prospects for molecular ventricular assistance. 
  • Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy. 
  • Isolation of retinal proteins that interact with retinitis pigmentosa GTPase regulator by interaction trap screen in yeast. 
  • Kinetics of bisulfite-induced cytosine deamination in single-stranded DNA. 
  • LexA protein of Escherichia coli represses expression of the Tn5 transposase gene. 
  • Local sources of retinoic acid coincide with retinoid-mediated transgene activity during embryonic development. 
  • Lysosomal basification and decreased autophagic flux in oxidatively stressed trabecular meshwork cells: implications for glaucoma pathogenesis. 
  • MBP-lacZ transgene expression in juvenile and adult Trembler-J mice. 
  • Mesenchymal stem cells modified with Akt prevent remodeling and restore performance of infarcted hearts. 
  • Messenger RNA repair and restoration of protein function by spliceosome-mediated RNA trans-splicing. 
  • Model for focal demyelination of the spinal dorsal columns of transgenic MBP-LacZ mice by phototargeted ablation of oligodendrocytes. 
  • Multimeric C9 within C5b-9 is required for inner membrane damage to Escherichia coli J5 during complement killing. 
  • Multiple innate inflammatory responses induced after systemic adenovirus vector delivery depend on a functional complement system. 
  • Mutations in acid beta-galactosidase cause GM1-gangliosidosis in American patients. 
  • Myelin acquisition in the central nervous system of the mouse revealed by an MBP-Lac Z transgene. 
  • Naked DNA delivered intraportally expresses efficiently in hepatocytes. 
  • Nerve activity-dependent modulation of calcineurin signaling in adult fast and slow skeletal muscle fibers. 
  • Neurotrophins regulate dendritic growth in developing visual cortex. 
  • Odorant receptors govern the formation of a precise topographic map. 
  • Oxidative stress impact on barrier function of porcine angular aqueous plexus cell monolayers. 
  • Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase. 
  • Parent-specific expression of a human keratin 18/beta-galactosidase fusion gene in transgenic mice. 
  • Peptide elongation factor eEF1A-2/S1 expression in cultured differentiated myotubes and its protective effect against caspase-3-mediated apoptosis. 
  • Peripheral mobilization of recipient bone marrow-derived endothelial progenitor cells enhances pancreatic islet revascularization and engraftment after intraportal transplantation. 
  • Persistence and survival of autologous muscle derived cells versus bovine collagen as potential treatment of stress urinary incontinence. 
  • Persistence of attenuated rev genes in a human immunodeficiency virus type 1-infected asymptomatic individual. 
  • Polyspecific binding of Escherichia coli beta-galactosidase by murine antibodies to DNA. 
  • Potential for germ line transmission after intramyocardial gene delivery by adeno-associated virus. 
  • Production of recombinant adeno-associated viral vectors and use for in vitro and in vivo administration. 
  • Quantification of keratan sulfate in blood as a marker of cartilage catabolism. 
  • Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen. 
  • Role for hedgehog signaling in hepatic stellate cell activation and viability. 
  • SOS induction as an in vivo assay of enzyme-DNA interactions. 
  • Screening for nitric oxide-dependent protein-protein interactions. 
  • Simultaneous proteomic profiling of four different growth states of human fibroblasts, using amine-reactive isobaric tagging reagents and tandem mass spectrometry. 
  • Sparing effect of leptin on liver glycogen stores in rats during the fed-to-fasted transition. 
  • Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter. 
  • Spliceosome-mediated RNA trans-splicing as a tool for gene therapy. 
  • Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy. 
  • Study of Cryptococcus neoformans actin gene regulation with a beta-galactosidase-actin fusion. 
  • Systemic production of human granulocyte colony-stimulating factor in nonhuman primates by transplantation of genetically modified myoblasts. 
  • Targeted gene transfer to Schlemm's canal by retroperfusion. 
  • Tethering ribozymes to a retroviral packaging signal for destruction of viral RNA. 
  • The Drosophila hindgut lacks constitutively active adult stem cells but proliferates in response to tissue damage. 
  • The SV40 core sequence functions as a repressor element in yeast. 
  • The end of the beginning. Gene transfer into the vessel wall. 
  • The epsilon subunit of DNA polymerase III Is involved in the nalidixic acid-induced SOS response in Escherichia coli. 
  • The human plasminogen activator inhibitor type I gene promoter targets to kidney. 
  • The interaction of nitric oxide (NO) with the yeast transcription factor Ace1: A model system for NO-protein thiol interactions with implications to metal metabolism. 
  • The role of the forkhead transcription factor, Foxc1, in the development of the mouse lacrimal gland. 
  • The suppression of testis-brain RNA binding protein and kinesin heavy chain disrupts mRNA sorting in dendrites. 
  • Transduction of dendritic cells by DNA viral vectors directs the immune response to transgene products in muscle fibers. 
  • Transgene expression in the brain stem effected by intramuscular injection of polyethylenimine/DNA complexes. 
  • Transgenic indicator mice for studying activated retinoic acid receptors during development. 
  • Translation limits synthesis of an assembly-initiating coat protein of filamentous phage IKe. 
  • Transmyocardial laser revascularization limits in vivo adenoviral-mediated gene transfer in porcine myocardium. 
  • Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types. 
  • Transplantation of multipotent progenitors from the adult olfactory epithelium. 
  • Transrectal gene therapy of the prostate in the canine model. 
  • Tumor-dendritic cell fusion as a basis for cancer immunotherapy. 
  • Use of lacZ expression to monitor transcription. 
  • Vascular-mesenchymal cross-talk through Vegf and Pdgf drives organ patterning. 
  • beta-cell function in normal rats made chronically hyperleptinemic by adenovirus-leptin gene therapy. 
  • bullwinkle is required for epithelial morphogenesis during Drosophila oogenesis. 
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  Keywords of People

  • Hogan, Brigid L. M., George Barth Geller Distinguished Professor of Immunology, Cell Biology
  • Liton, Paloma Borrajo, Associate Professor in Ophthalmology, Pathology
  • Milano, Carmelo Alessio, Professor of Surgery, Surgery, Cardiovascular and Thoracic Surgery
  • Stinnett, Sandra Sue, Associate Professor of Biostatistics and Bioinformatics, Biostatistics & Bioinformatics