Biological Assay

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  Subject Areas on Research

  • A 'Semi-Protected Oligonucleotide Recombination' Assay for DNA Mismatch Repair in vivo Suggests Different Modes of Repair for Lagging Strand Mismatches. 
  • A compact CMOS biochip immunosensor towards the detection of a single bacteria. 
  • A comparative study of the non-acidic chemically mediated antifoulant properties of three sympatric species of ascidians associated with seagrass habitats. 
  • A continental view of pine-associated ectomycorrhizal fungal spore banks: a quiescent functional guild with a strong biogeographic pattern. 
  • A critical body residue approach for predicting persistent bioaccumulative toxicant effects on reproduction and population dynamics of meiobenthic copepods. 
  • A functional assay-based strategy for nanomaterial risk forecasting. 
  • A modified host-cell reactivation assay to measure repair of alkylating DNA damage for assessing risk of lung adenocarcinoma. 
  • A preliminary investigation of the parallelogram concept in genetic monitoring and risk estimation. 
  • A prochelator with a modular masking group featuring hydrogen peroxide activation with concurrent fluorescent reporting. 
  • A rationally designed heparin, M118, has anticoagulant activity similar to unfractionated heparin and different from Lovenox in a cell-based model of thrombin generation. 
  • ACTH receptors in the adrenal: specific binding of ACTH-125I and its relation to adenyl cyclase. 
  • Abnormal production of interleukin-1 by microglia from trisomy 16 mice. 
  • Activation of Human Peroxisome Proliferator-Activated Nuclear Receptors (PPARγ1) by Semi-Volatile Compounds (SVOCs) and Chemical Mixtures in Indoor Dust. 
  • Active site-inactivated factors VIIa, Xa, and IXa inhibit individual steps in a cell-based model of tissue factor-initiated coagulation. 
  • Alteration in sphingolipid metabolism: bioassays for fumonisin- and ISP-I-like activity in tissues, cells and other matrices. 
  • An adaptable mesocosm platform for performing integrated assessments of nanomaterial risk in complex environmental systems. 
  • An artificial fertilization method with the Japanese medaka: implications in early life stage bioassays and solvent toxicity. 
  • An entirely cell-based system to generate single-chain antibodies against cell surface receptors. 
  • An enzyme-linked immunosorbent assay for estrogenicity using primary hepatocyte cultures from the channel catfish (Ictalurus punctatus). 
  • An in vitro bioassay for neurite growth using cryostat sections of nervous tissue as a substratum. 
  • Antiulcer Agents. 1. Gastric Antisecretory and Cytoprotective Properties of Substituted Imidzao[1,2-α]pyridines 
  • Application of in vivo and in vitro pharmacokinetics for physiologically relevant drug exposure in a human tumor clonogenic cell assay. 
  • Aquaporin in Candida: characterization of a functional water channel protein. 
  • Assays for recombinant proteins: a problem in non-linear calibration. 
  • Assessment of the Feasibility of Using Noninvasive Wearable Biometric Monitoring Sensors to Detect Influenza and the Common Cold Before Symptom Onset. 
  • Bayesian inferences on umbrella orderings. 
  • Bioassay of plasma cholecystokinin in rats: effects of food, trypsin inhibitor, and alcohol. 
  • Calmodulin. Development and application of a sensitive radioimmunoassay. 
  • Cardiac cell therapy in vitro: reproducible assays for comparing the efficacy of different donor cells. 
  • Caveats to the use of MTT, neutral red, Hoechst and Resazurin to measure silver nanoparticle cytotoxicity. 
  • Characterization of the recalcitrant CYP1 phenotype found in Atlantic killifish (Fundulus heteroclitus) inhabiting a Superfund site on the Elizabeth River, VA. 
  • Characterizing the peroxisome proliferator-activated receptor (PPARγ) ligand binding potential of several major flame retardants, their metabolites, and chemical mixtures in house dust. 
  • Chemical Mixtures Isolated from House Dust Disrupt Thyroid Receptor β Signaling. 
  • Cholecystokinin bioactivity in human plasma. Molecular forms, responses to feeding, and relationship to gallbladder contraction. 
  • Circulating mitochondria in deceased organ donors are associated with immune activation and early allograft dysfunction. 
  • Comparison of PF4/heparin ELISA assay with the 14C-serotonin release assay in the diagnosis of heparin-induced thrombocytopenia. 
  • Comparison of a chemical assay with a tissue-mediated bio-assay for chemical mutagens. 
  • Concerted regulation of skeletal muscle contractility by oxygen tension and endogenous nitric oxide. 
  • Constrained four parameter logistic model. 
  • Coordination of DNA ends during double-strand-break repair in bacteriophage T4. 
  • Cryptococcus neoformans Kin1 protein kinase homologue, identified through a Caenorhabditis elegans screen, promotes virulence in mammals. 
  • DNA bioassay-on-chip using SERS detection for dengue diagnosis. 
  • DSC1 channel-dependent developmental regulation of pyrethroid susceptibility in Drosophila melanogaster. 
  • Detection of Escherichia coli enterotoxins in stools. 
  • Determination of Real Time in Vivo Drug Receptor Occupancy for a Covalent Binding Drug as a Clinical Pharmacodynamic Biomarker by Immunocapture-LC-MS/MS. 
  • Development and validation of a cell-based fluorescent method for measuring antibody affinity. 
  • Diagnostic accuracy of leptospirosis whole-cell lateral flow assays: a systematic review and meta-analysis. 
  • Dioxinlike properties of a trichloroethylene combustion-generated aerosol. 
  • Discovery of high-affinity peptide binders to BLyS by phage display. 
  • Disparate results between proliferation rates of surgically excised prostate tumors and an in vitro bioassay using sera from a positive randomized controlled trial. 
  • Dose response of angiogenesis to basic fibroblast growth factor in rat corneal pocket assay: I. Experimental characterizations. 
  • Dose response of angiogenesis to basic fibroblast growth factor in rat corneal pocket assay: II. Numerical simulations. 
  • Efficacy of High-Sensitivity Troponin T in Identifying Very-Low-Risk Patients With Possible Acute Coronary Syndrome. 
  • Embryonic chick cartilage produces its own somatomedin-like peptide to stimulate cartilage growth in vitro. 
  • Estrogen levels in childhood determined by an ultrasensitive recombinant cell bioassay. 
  • Evaluation of a recombinant yeast cell estrogen screening assay. 
  • Evaluation of fish models of soluble epoxide hydrolase inhibition. 
  • Expression of tumor necrosis factor receptor-1 in arterial wall cells promotes atherosclerosis. 
  • Expression, purification and primary crystallographic study of human androgen receptor in complex with DNA and coactivator motifs. 
  • Factors in standardizing automated cholinesterase assays. 
  • Flow cytometry analysis of adenosine deaminase (ADA) expression: a simple and reliable tool for the assessment of ADA-deficient patients before and after gene therapy. 
  • Fluid shear stress induces endothelial transforming growth factor beta-1 transcription and production. Modulation by potassium channel blockade. 
  • Four-parameter paired response curve for serial dilution assays. 
  • Functional analysis of a complementary DNA for the 50-kilodalton subunit of calmodulin kinase II. 
  • Functional analysis of a mammalian odorant receptor subfamily. 
  • Functional assay to guide precision radiotherapy by assessing individual patient radiosensitivity. 
  • Further assessment of an in vitro screen that may help identify organophosphorus pesticides that are more acutely toxic to the young. 
  • Guidelines for the development and validation of new potency assays for the evaluation of umbilical cord blood. 
  • Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). 
  • Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1. 
  • Guidelines for the use and interpretation of assays for monitoring autophagy. 
  • Hemoglobin conformation couples erythrocyte S-nitrosothiol content to O2 gradients. 
  • High-content profiling of cell responsiveness to graded substrates based on combinyatorially variant polymers. 
  • High-resolution probing of cellular force transmission. 
  • Human olfactory receptor responses to odorants. 
  • Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery 
  • Identification and expression of the ecdysone receptor in the harpacticoid copepod, Amphiascus tenuiremis, in response to fipronil. 
  • Identification of a peripheral nerve neurite growth-promoting activity by development and use of an in vitro bioassay. 
  • In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins. 
  • Interlaboratory validation of a new assay for DNA-protein crosslinks. 
  • International network for comparison of HIV neutralization assays: the NeutNet report II. 
  • International, multicenter evaluation of a new D-dimer assay for the exclusion of venous thromboembolism using standard and age-adjusted cut-offs. 
  • Investigating the Sensitivity of NAD+-dependent Sirtuin Deacylation Activities to NADH. 
  • Isolation and insecticidal activity of mellamide from Aspergillus melleus. 
  • Kinetic approaches to study the function of RGS9 isoforms. 
  • Labeling of peripheral blood polymorphonuclear leukocytes with indium-111: a new method for the quantitation of in-vivo accumulation of PMNLs in rabbit skin. 
  • Life-cycle effects of single-walled carbon nanotubes (SWNTs) on an estuarine meiobenthic copepod. 
  • Local sources of retinoic acid coincide with retinoid-mediated transgene activity during embryonic development. 
  • Long-term treatment with botulinum toxin type A in cervical dystonia has low immunogenicity by mouse protection assay. 
  • Machine Learning Uncovers Food- and Excipient-Drug Interactions. 
  • Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish. 
  • Methods for embryo dissociation and analysis of cell adhesion. 
  • Molecular assays for antimalarial drug resistance surveillance: A target product profile. 
  • Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip. 
  • Nerve growth factor and its low-affinity receptor promote Schwann cell migration. 
  • Neurotrophins affect the pattern of DRG neurite growth in a bioassay that presents a choice of CNS and PNS substrates. 
  • Off the Shelf Fouling Management. 
  • Optical coding of mammalian cells using semiconductor quantum dots. 
  • Periodic root branching in Arabidopsis requires synthesis of an uncharacterized carotenoid derivative. 
  • Pharmaceuticals as antifoulants: concept and principles. 
  • Plasmonic assay for amplification-free cancer biomarkers detection in clinical tissue samples. 
  • Polymorphonuclear leukocytes: possible mechanism of accumulation in psoriasis. 
  • Potency analysis of cellular therapies: the emerging role of molecular assays. 
  • Preparation of polyethylene glycol-tissue plasminogen activator adducts that retain functional activity: characteristics and behavior in three animal species. 
  • Problems for risk assessment of endocrine-active estrogenic compounds. 
  • Prognostic implications of low level cardiac troponin elevation using high-sensitivity cardiac troponin T. 
  • Prognostic performance of a high-sensitivity cardiac troponin I assay in patients with non-ST-elevation acute coronary syndrome. 
  • Proteinases of human epidermis; a possible mechanism for polymorphonuclear leukocyte chemotaxis. 
  • Protocol to measure endotoxin from opaque tissues in mice using an optimized kinetic limulus amebocyte lysate assay. 
  • Purification and characterization of a luminal cholecystokinin-releasing factor from rat intestinal secretion. 
  • Quantitative assessment of growth stimulating activity of the vitreous during PVR. 
  • Quantitative comparison of the inhibitory effects of GW5638 and tamoxifen on angiogenesis in the cornea pocket assay. 
  • Radiation injury treatment network (RITN): healthcare professionals preparing for a mass casualty radiological or nuclear incident. 
  • Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma. 
  • Rapid Nanophotonics Assay for Head and Neck Cancer Diagnosis. 
  • Reaction of N-acylhomoserine lactones with hydroxyl radicals: rates, products, and effects on signaling activity. 
  • Response to 'Antinuclear antibodies by indirect immunofluorescence and solid phase assays' by Bossuyt et al. 
  • Response to: "Antinuclear autoantibodies: discordance among four different assays" by Pacheco et al. 
  • Response to: 'Antinuclear antibody as entry criterion for classification of systemic lupus erythematosus: pitfalls and opportunities' by Bossuyt et al. 
  • Response to: 'Can solid-phase assays replace immunofluorescence for ANA screening?' by Bizzaro. 
  • Response to: 'Pitfalls of antinuclear antibody detection in systemic lupus erythematosus: the positive experience of a national multi-center study' by Pregnalato et al. 
  • Response to: 'Unending story of the indirect immunofluorescence assay on HEp-2 cells: old problems and new solutions?' by Meroni et al. 
  • Response to: 'Variation in antinuclear antibody detection by automated indirect immunofluorescence analysis' by van Hoovels et al. 
  • SERS nanosensors and nanoreporters: golden opportunities in biomedical applications. 
  • Somatostatin-like immunoactivity and biological activity is present in Tetrahymena pyriformis, a ciliated protozoan. 
  • Split luciferase complementation assay for the analysis of G protein-coupled receptor ligand response in Saccharomyces cerevisiae. 
  • Stage-specific toxicity of cypermethrin to medaka (Oryzias latipes) eggs and embryos using a refined methodology for an in vitro fertilization bioassay. 
  • Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay. 
  • Systematic errors in detecting biased agonism: Analysis of current methods and development of a new model-free approach. 
  • Technologies for Frugal and Sensitive Point-of-Care Immunoassays. 
  • The 99th percentile upper reference limit for the 5th generation cardiac troponin T assay in the United States. 
  • The Alzheimer's Association external quality control program for cerebrospinal fluid biomarkers. 
  • The clinical need for clustered AChR cell-based assay testing of seronegative MG. 
  • The hypogonadotropic state of the prepubertal male rhesus monkey (Macaca mulatta) is not associated with a decrease in hypothalamic gonadotropin-releasing hormone content. 
  • Total synthesis, purification, and characterization of human [Phe(p-CH2SO 3Na)52, Nle32,53,56, Nal55]-CCK20-58, [Tyr52, Nle32,53,56, Nal55]-CCK-58, and [Phe(p-CH2SO3Na)52, Nle32,53,56, Nal55]-CCK-58. 
  • Tracking complex mixtures of chemicals in our changing environment. 
  • Understanding the characteristics of nonspecific binding of drug-like compounds to canonical stem-loop RNAs and their implications for functional cellular assays. 
  • Update on EPA's ToxCast program: providing high throughput decision support tools for chemical risk management. 
  • Use of alternative bioassays to explore the impact of pyrethroid resistance on LLIN efficacy. 
  • Vitamin D(3) cryosensitization increases prostate cancer susceptibility to cryoablation via mitochondrial-mediated apoptosis and necrosis. 
  • Water movement during apoptosis: a role for aquaporins in the apoptotic volume decrease (AVD). 
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  Keywords of People

  • Ginsburg, Geoffrey Steven, Adjunct Professor in the Department of Medicine, School of Nursing
  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Biomedical Engineering