Cell Extracts
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Subject Areas on Research
- A fluorescent assay for agonist-activated phospholipase D in mammalian cell extracts.
- Activated α2-macroglobulin binding to cell surface GRP78 induces T-loop phosphorylation of Akt1 by PDK1 in association with Raptor.
- An in vitro transcription system that recapitulates equine infectious anemia virus tat-mediated inhibition of human immunodeficiency virus type 1 Tat activity demonstrates a role for positive transcription elongation factor b and associated proteins in the mechanism of Tat activation.
- Analysis of apoptosis using Xenopus egg extracts.
- Analysis of the cell cycle using Xenopus egg extracts.
- Appearance of protease activities coincides with p10 and polyhedrin-driven protein production in the baculovirus expression system: effects on yield.
- Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors.
- Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro.
- CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription.
- Cell-type-specific repression of internal ribosome entry site activity by double-stranded RNA-binding protein 76.
- Chaperones rescue luciferase folding by separating its domains.
- Complementation of SOD-deficient Escherichia coli by manganese porphyrin mimics of superoxide dismutase activity.
- Detection of protein S-nitrosylation with the biotin-switch technique.
- ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system.
- Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase.
- FGF2 suppresses neuronogenesis of a cell line derived from rat olfactory epithelium.
- Glucocorticoid receptor binding to rat liver nuclei occurs without nuclear transport.
- Human melanoma-mediated inhibition of autologous CD4+ helper tumor-infiltrating lymphocyte growth in vitro.
- Identification of dengue RNA binding proteins using RNA chromatography and quantitative mass spectrometry.
- Identifying phosphoCTD-associating proteins.
- In vitro and tissue culture methods for analysis of translation initiation on the endoplasmic reticulum.
- Insulin-like growth factor-I is expressed by avian flexor tendon cells.
- Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.
- Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components.
- Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx.
- Physiologic response to chronic house dust mite exposure in mice is dependent on lot characteristics.
- Preemptive Tolerogenic Delivery of Donor Antigens for Permanent Allogeneic Islet Graft Protection.
- Preparation and use of interphase Xenopus egg extracts.
- Production of progestin-stimulatory factor(s) by enriched populations of rat T and B lymphocytes.
- Protein phosphatase 2A and its B56 regulatory subunit inhibit Wnt signaling in Xenopus.
- RB-mediated tumor suppression of a lung cancer cell line is abrogated by an extract enriched in extracellular matrix.
- RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts.
- Recognition and repair of compound DNA lesions (base damage and mismatch) by human mismatch repair and excision repair systems.
- Regulation of apoptosis in Xenopus egg extracts.
- Scythe: a novel reaper-binding apoptotic regulator.
- Sp1 activation of a TATA-less promoter requires a species-specific interaction involving transcription factor IID.
- Study of apoptosis in vitro using the Xenopus egg extract reconstitution system.
- Superoxide dismutase and catalase of calf trabecular meshwork.
- The dishevelled protein is modified by wingless signaling in Drosophila.
- The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1.
- Wee1-regulated apoptosis mediated by the crk adaptor protein in Xenopus egg extracts.