Cell Extracts

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  Subject Areas on Research

  • A fluorescent assay for agonist-activated phospholipase D in mammalian cell extracts. 
  • Activated α2-macroglobulin binding to cell surface GRP78 induces T-loop phosphorylation of Akt1 by PDK1 in association with Raptor. 
  • An in vitro transcription system that recapitulates equine infectious anemia virus tat-mediated inhibition of human immunodeficiency virus type 1 Tat activity demonstrates a role for positive transcription elongation factor b and associated proteins in the mechanism of Tat activation. 
  • Analysis of apoptosis using Xenopus egg extracts. 
  • Analysis of the cell cycle using Xenopus egg extracts. 
  • Appearance of protease activities coincides with p10 and polyhedrin-driven protein production in the baculovirus expression system: effects on yield. 
  • Assessing acetylation of NF-kappaB. 
  • Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors. 
  • Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro. 
  • CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription. 
  • Cell-type-specific repression of internal ribosome entry site activity by double-stranded RNA-binding protein 76. 
  • Chaperones rescue luciferase folding by separating its domains. 
  • Complementation of SOD-deficient Escherichia coli by manganese porphyrin mimics of superoxide dismutase activity. 
  • Detection of protein S-nitrosylation with the biotin-switch technique. 
  • ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system. 
  • Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase. 
  • FGF2 suppresses neuronogenesis of a cell line derived from rat olfactory epithelium. 
  • Glucocorticoid receptor binding to rat liver nuclei occurs without nuclear transport. 
  • Human melanoma-mediated inhibition of autologous CD4+ helper tumor-infiltrating lymphocyte growth in vitro. 
  • Identification of dengue RNA binding proteins using RNA chromatography and quantitative mass spectrometry. 
  • Identifying phosphoCTD-associating proteins. 
  • In vitro and tissue culture methods for analysis of translation initiation on the endoplasmic reticulum. 
  • Insulin-like growth factor-I is expressed by avian flexor tendon cells. 
  • Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation. 
  • Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components. 
  • Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx. 
  • Physiologic response to chronic house dust mite exposure in mice is dependent on lot characteristics. 
  • Preemptive Tolerogenic Delivery of Donor Antigens for Permanent Allogeneic Islet Graft Protection. 
  • Preparation and use of interphase Xenopus egg extracts. 
  • Production of progestin-stimulatory factor(s) by enriched populations of rat T and B lymphocytes. 
  • Protein phosphatase 2A and its B56 regulatory subunit inhibit Wnt signaling in Xenopus. 
  • RB-mediated tumor suppression of a lung cancer cell line is abrogated by an extract enriched in extracellular matrix. 
  • RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts. 
  • Recognition and repair of compound DNA lesions (base damage and mismatch) by human mismatch repair and excision repair systems. 
  • Regulation of Clara cell secretory protein gene transcription by thyroid transcription factor-1. 
  • Regulation of apoptosis in Xenopus egg extracts. 
  • Scythe: a novel reaper-binding apoptotic regulator. 
  • Simultaneous protein expression and modification: an efficient approach for production of unphosphorylated and biotinylated receptor tyrosine kinases by triple infection in the baculovirus expression system. 
  • Sp1 activation of a TATA-less promoter requires a species-specific interaction involving transcription factor IID. 
  • Study of apoptosis in vitro using the Xenopus egg extract reconstitution system. 
  • Superoxide dismutase and catalase of calf trabecular meshwork. 
  • The dishevelled protein is modified by wingless signaling in Drosophila. 
  • The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1. 
  • Wee1-regulated apoptosis mediated by the crk adaptor protein in Xenopus egg extracts.