Cell-Free System

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  Subject Areas on Research

  • A defined human system that supports bidirectional mismatch-provoked excision. 
  • A miRNA-responsive cell-free translation system facilitates isolation of hepatitis C virus miRNP complexes. 
  • A rapid method for the isolation and partial purification of specific eucaryotic messenger RNA's. 
  • A recellularized human colon model identifies cancer driver genes. 
  • Acetyl-CoA:1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase is directly activated by p38 kinase. 
  • Activation of the respiratory burst enzyme from human neutrophils in a cell-free system. Evidence for a soluble cofactor. 
  • Administration of atrial natriuretic factor inhibits sodium-coupled transport in proximal tubules. 
  • Allosteric effects on oxidative and nitrosative reactions of cell-free hemoglobins. 
  • Alteration by methadone of catecholamine uptake and release in isolated rat adrenomedullary storage vesicles. 
  • An in vitro transcription system that recapitulates equine infectious anemia virus tat-mediated inhibition of human immunodeficiency virus type 1 Tat activity demonstrates a role for positive transcription elongation factor b and associated proteins in the mechanism of Tat activation. 
  • Apoptosis in Xenopus egg extracts. 
  • Apoptosis induction by caspase-8 is amplified through the mitochondrial release of cytochrome c. 
  • Appearance of a ribonucleic acid polymerase-binding protein in asporogenous mutants of Bacillus subtilis. 
  • Beta-adrenergic receptors: regulatory role of agonists. 
  • Cell-free and erythrocytic S-nitrosohemoglobin inhibits human platelet aggregation. 
  • Cell-free heterologous desensitization of adenylyl cyclase in S49 lymphoma cell membranes mediated by cAMP-dependent protein kinase. 
  • Characterization of lens fiber cell triton insoluble fraction reveals ERM (ezrin, radixin, moesin) proteins as major cytoskeletal-associated proteins. 
  • Depression of macrophage function by a factor produced by neoplasms: a merchanism for abrogation of immune surveillance. 
  • Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides. 
  • Desensitization of the turkey erythrocyte beta-adrenergic receptor in a cell-free system. Evidence that multiple protein kinases can phosphorylate and desensitize the receptor. 
  • Developmental neurotoxicity of chlorpyrifos in vivo and in vitro: effects on nuclear transcription factors involved in cell replication and differentiation. 
  • Effect of anti-V3 antibodies on cell-free and cell-to-cell human immunodeficiency virus transmission. 
  • Effect of cyclic adenosine 3',5'-monophosphate on the incorporation of 3H-leucine into polypeptides by beef thyroid polysomes in vitro. 
  • Effects of nickel(II) on nuclear protein binding to DNA in intact mammalian cells. 
  • Ethanol stimulates gamma-aminobutyric acid receptor-mediated chloride transport in rat brain synaptoneurosomes. 
  • Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide. 
  • Functional inactivation and structural disruption of human alpha 2-macroglobulin by neutrophils and eosinophils. 
  • Genetic recombination of poliovirus in a cell-free system. 
  • HIV-1 Rev promotes the nuclear export of unspliced and singly spliced RNAs in a mammalian cell-free export system. 
  • Heat shock protein 96 is elevated in rheumatoid arthritis and activates macrophages primarily via TLR2 signaling. 
  • High and low molecular weight DNA cleavage in ovarian granulosa cells: characterization and protease modulation in intact cells and in cell-free nuclear autodigestion assays. 
  • Hormones, receptors, and cyclic AMP: their role in target cell refractoriness. 
  • Human thymic epithelial cells produce granulocyte and macrophage colony-stimulating factors. 
  • Hypoxia impedes the formation of chromium DNA-adducts in a cell-free system. 
  • Identification of a novel stage of ribosome/nascent chain association with the endoplasmic reticulum membrane. 
  • In vitro biosynthesis of plasminogen in a cell-free system directed by mRNA fractions isolated from monkey liver. 
  • In vitro translation of RNA from the German cockroach Blattella germanica. 
  • Induction of specific cytochrome P-450 isozymes by methylenedioxyphenyl compounds and antagonism by 3-methylcholanthrene. 
  • Induction of specific translatable messenger RNA's by oestrogen and progesterone. 
  • Inflammation and viral infection: chemotactic activity resulting from the interaction of antiviral antibody and complement with cells infected with herpes simplex virus. 
  • Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA. 
  • Interaction of a 60-kilodalton D-mannose-containing salivary glycoprotein with type 1 fimbriae of Escherichia coli. 
  • Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts. 
  • Is oxidative stress involved in the developmental neurotoxicity of chlorpyrifos? 
  • Isolation of a transplantable cell line induced by the MC29 avian leukosis virus. 
  • Isolation of polyribosomes and characteristics of an amino acid-incorporation system from the liver of a primate (Papio papio). 
  • Isolation of protein factors from oviduct polysomes which stimulate protein synthesis. 
  • La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate. 
  • Localization of an aminoacridine antitumor agent in a type II topoisomerase-DNA complex. 
  • Mechanism of the luminol-dependent chemiluminescence of human neutrophils. 
  • Mechanisms of chromium-induced suppression of RNA synthesis in cellular and cell-free systems: relationship to RNA polymerase arrest. 
  • Membrane insertion, glycosylation, and oligomerization of inositol trisphosphate receptors in a cell-free translation system. 
  • Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells. 
  • Novel cell-free strategy for therapeutic angiogenesis: in vitro generated conditioned medium can replace progenitor cell transplantation. 
  • On the ultrastructure of hyalin, a cell adhesion protein of the sea urchin embryo extracellular matrix. 
  • Oxidant-induced cell death in retinal pigment epithelium cells mediated through the release of apoptosis-inducing factor. 
  • Phosphorylation-dependent activation of the adenovirus-inducible E2F transcription factor in a cell-free system. 
  • Protein biosynthesis on chick oviduct polyribosomes. I. Changes during estrogen-mediated tissue differentiation. 
  • Protein bisoynthesis in the testis. IV. Isolation and properties of polyribosomes. 
  • RANBP2 is an allosteric activator of the conventional kinesin-1 motor protein, KIF5B, in a minimal cell-free system. 
  • Reactive oxygen species produced by liver mitochondria of rats in sepsis. 
  • Reconstitution of functional membrane-bound acetylcholine receptor from isolated Torpedo californica receptor protein and electroplax lipids. 
  • Reconstitution of simian virus 40 DNA replication with purified proteins. 
  • Simian virus 40 transcriptional complexes incorporate mercurated nucleotides into RNA in vitro. 
  • Stimulation of cell-free polypeptide synthesis by a protein fraction extracted from chick oviduct polyribosomes. 
  • Structure and function of the glycosaminoglycan binding site of chemokine macrophage-inflammatory protein-1 beta. 
  • Studies of the biosynthesis of renin with a cell-free translation system. 
  • Substrate spectrum of human excinuclease: repair of abasic sites, methylated bases, mismatches, and bulky adducts. 
  • Suppression of DNA-damage checkpoint signaling by Rsk-mediated phosphorylation of Mre11. 
  • Synthetic biology looks good on paper. 
  • The effect of cell-free and erythrocyte-containing perfusion in rat livers. 
  • The effect of rhodopsin phosphorylation on the light-dependent activation of phosphodiesterase from bovine rod outer segments. 
  • The human glutathione S-transferase P1 protein is phosphorylated and its metabolic function enhanced by the Ser/Thr protein kinases, cAMP-dependent protein kinase and protein kinase C, in glioblastoma cells. 
  • The initial nucleotide sequence of DNA transcribed from avian myeloblastosis virus 70 S RNA by RNA-dependent DNA polymerase. 
  • The mechanism of cancer-mediated conversion of plasminogen to the angiogenesis inhibitor angiostatin. 
  • The retinitis pigmentosa GTPase regulator (RPGR) interacts with novel transport-like proteins in the outer segments of rod photoreceptors. 
  • The role of ultrasound in women who undergo cell-free DNA screening. 
  • The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively. 
  • Tyrosine phosphorylation of the human glutathione S-transferase P1 by epidermal growth factor receptor. 
  • Use of the Comet assay to investigate the role of superoxide in glutathione-induced DNA damage. 
  • Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation.