Centrifugation, Density Gradient

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  Subject Areas on Research

  • A human mononuclear leukocyte chemotactic factor: characterization, specificity and kinetics of production by homologous leukocytes. 
  • A rapid method to isolate platelets from human blood by density gradient centrifugation. 
  • Activation of phenylalanine hydroxylase by phenylalanine. 
  • Advanced lipoprotein testing does not improve identification of subclinical atherosclerosis in young adults: the Bogalusa Heart Study. 
  • All-or-none secretion of adrenal medullary storage vesicle contents in the rat. 
  • Analysis of Fc gamma receptors on human peripheral blood leukocytes by flow microfluorometry. I. Receptor distributions on monocytes, T gamma cells and cells labeled with the 3Al anti-T cell monoclonal antibody. 
  • Apoptosis in Xenopus egg extracts. 
  • Are mycoplasma membrane proteins affected by variations in membrane fatty acid composition? 
  • Assembly of translocation-competent proteoliposomes from detergent-solubilized rough microsomes. 
  • Assessment of sex steroid action in vitro. 
  • Association of the viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids. 
  • Brevetoxin-6 (PbTx-6), a nonaromatic marine neurotoxin, is a ligand of the aryl hydrocarbon receptor. 
  • Characteristics of GTP-mediated microsomal Ca2+ release. 
  • Characterization of molybdenum cofactor from Escherichia coli. 
  • Chemotactic and anaphylatoxic fragment cleaved from the fifth component of guinea pig complement. 
  • Circulating IgA immune complexes in patients with psoriasis. 
  • Cytochemistry of sex steroid receptors: a critique. 
  • DNA repair in human leukaemic lymphocytes. 
  • DNA repair synthesis after x-irradiation in E. coli. 
  • DNA synthesis in strains of Escherichia coli K12 with temperature-sensitive DNA ligase and DNA polymerase I. 
  • Differential replication of satellite DNA in polyploid tissues of Drosophila virilis. 
  • Dysregulation of proteoglycan production by intrahepatic biliary epithelial cells bearing defective (delta-f508) cystic fibrosis transmembrane conductance regulator. 
  • Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. VI. Newly synthesized proteins in the T4 protein-deoxyribonucleic acid complex. 
  • Effect of cyclic adenosine 3',5'-monophosphate on the incorporation of 3H-leucine into polypeptides by beef thyroid polysomes in vitro. 
  • Electron microscopy and hydrodynamic properties of factor XIII subunits. 
  • Enzymatic joining of polynucleotides. XI. Reversal of Escherichia coli deoxyribonucleic acid ligase reaction. 
  • Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum. 
  • Ex vivo expansion, maturation, and activation of umbilical cord blood-derived T lymphocytes with IL-2, IL-12, anti-CD3, and IL-7. Potential for adoptive cellular immunotherapy post-umbilical cord blood transplantation. 
  • Expression of cDNAs for G proteins in Escherichia coli. Two forms of Gs alpha stimulate adenylate cyclase. 
  • Expression, purification, and in vitro assays of mitochondrial single-stranded DNA-binding protein. 
  • Extracellular trafficking of myocilin in human trabecular meshwork cells. 
  • Fibronectin in extended and compact conformations. Electron microscopy and sedimentation analysis. 
  • Further characterization of a factor from endotoxin-treated serum which releases histamine and heparin from mast cells. 
  • Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase. 
  • Genetic, biochemical, and structural studies of biogenesis of adhesive pili in bacteria. 
  • Heterogeneity of endosomal populations in the rat renal cortex: light endosomes. 
  • Identification of casein kinase II and phosphorylated proteins associated with messenger ribonucleoproteins particles from reticulocytes. 
  • Identification, characterization, and functional correlation of calmodulin-dependent protein phosphatase in sperm. 
  • IgA-containing circulating immune complexes in gluten-sensitive enteropathy. 
  • In vitro synthesis of phi-X-174 single-stranded DNA. 
  • In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4. 
  • Induction of micronuclei by X-radiation in human, mouse and rat peripheral blood lymphocytes. 
  • Isolation and characterization of C4-synthesizing cells from guinea-pig spleen. 
  • Isolation and characterization of two group-specific antigens from feline leukemia virus. 
  • Isolation of oligodendrocyte-like cells from human umbilical cord blood. 
  • Isolation of polyribosomes and characteristics of an amino acid-incorporation system from the liver of a primate (Papio papio). 
  • Isolation of protein factors from oviduct polysomes which stimulate protein synthesis. 
  • Isolation of proteins by gel filtration in 6M guanidinium chloride: application to RNA tumor viruses. 
  • Isolation of renal brush borders. 
  • Kinematic response of human spermatozoa to nonoxynol-9. 
  • Lipid environment of acetylcholine receptor from Torpedo californica. 
  • Lipoprotein-like particles and cholesteryl esters in human Bruch's membrane: initial characterization. 
  • Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures. 
  • Localization of functionally different guinea-pig spleen cells in albumin gradients. 
  • Lymphopenic immunologic deficiency in identical twins: lymphocyte allografting and graft-versus-host disease following treatment with albumin-gradient-separated paternal bone marrow cells. 
  • Malignant effusions in recurrent breast cancer: steroid hormone receptor analysis of effusion fluid derived cells. 
  • Maturation of the adrenal medulla. I. Uptake and storage of amines in isolated storage vesicles of the rat. 
  • Maturation of the adrenal medulla. II. Content and properties of catecholamine storage vesicles of the rat. 
  • Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome. 
  • Molecular form of adenosine deaminase in severe combined immunodeficiency. 
  • Multiple and specific initiation of T4 DNA replication. 
  • Novel large apolipoprotein E-containing lipoproteins of density 1.006-1.060 g/ml in human cerebrospinal fluid. 
  • Nuclear RNA synthesis in sea urchin embryos. 
  • Outer membrane of avian myeloblastosis virus. 
  • Ovalbumin messenger RNA of chick oviduct: partial characterization, estrogen dependence, and translation in vitro. 
  • Pericardial cells for graft seeding: isolation, culture and identification. 
  • Phosphoinositides of sheep platelets plasma membranes. 
  • Physical and chemical properties of rabbit muscle phosphofructokinase cross-linked with dimethyl suberimidate. 
  • Polypeptides of avian RNA tumor viruses. 1. Isolation and physical and chemical analysis. 
  • Polypeptides of avian RNA tumor viruses. 3. Purification and identification of a DNA synthesizing enzyme. 
  • Polypeptides of avian RNA tumor viruses. IV. Components of the viral envelope. 
  • Polypeptides of avian RNA tumor viruses. V. Analysis of the virus core. 
  • Polypeptides of mammalian oncornaviruses. I. Isolation and serological analysis polypeptides from murine and feline C-type viruses. 
  • Preferential synthesis of yeast mitochondrial DNA in alpha factor-arrested cells. 
  • Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct. 
  • Properties of mouse leukemia viruses. II. Isolation of viral components. 
  • Protein biosynthesis in the testis. V. Concerning the mechanism of stimulation by follicle-stimulating hormone. 
  • Protein biosynthesis on chick oviduct polyribosomes. I. Changes during estrogen-mediated tissue differentiation. 
  • Protein biosynthesis on chick oviduct polyribosomes. II. Regulation by progesterone. 
  • Protein bisoynthesis in the testis. IV. Isolation and properties of polyribosomes. 
  • Recent and innovative methods for detection of bacteremia and fungemia. 
  • Recovery of rat adrenal amine stores after insulin administration. 
  • Regulation of translation and transcription of messenger RNA during early embryonic development. 
  • Relationship of age and menopausal status to estrogen receptor content in primary carcinoma of the breast. 
  • Replicating circular DNA molecules in yeast. 
  • Replication control of autonomously replicating human sequences. 
  • Replication of yeast chromosomal DNA. 
  • Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs. 
  • Rhipicephalus sanguineus: vector of a new spotted fever group rickettsia in the United States. 
  • Ribonucleic acid components of BAI strain A (myeloblastosis) avian tumor virus. 
  • Ribosomal RNA in avian leukosis virus particles. 
  • Ribosomal subunits of Landschütz ascites cells during changes in polysome distribution. 
  • Role of ATP in DNA synthesis in Escherichia coli. 
  • Satellite DNA sequences of Drosophila melanogaster. 
  • Secretory immune response in patients with oropharyngeal carcinoma. 
  • Sedimentation properties of yeast chromosomal DNA. 
  • Separation of two thyrotropin binding components from porcine thyroid tissue by affinity chromatography: characterization of high and low affinity sites. 
  • Sex steroid receptor concentration in breast carcinoma tissue: effect of devascularization during mastectomy. 
  • Simple and inexpensive fabrication of small-volume density gradients. 
  • Size and structure of yeast chromosomal DNA. 
  • Size differences among the high molecular weight RNA's of avian tumor viruses. 
  • Specific nuclear binding of adenosine 3',5'-monophosphate-binding protein complex with subsequent poly(A) RNA synthesis in embryonic chick cartilage. 
  • Structural components of avian myeloblastosis virus. 
  • Structure of human platelet membrane glycoproteins IIb and IIIa as determined by electron microscopy. 
  • Subcellular distribution of transmitter-related enzyme activities in discrete areas of the rat dentate gyrus. 
  • Substructure of human von Willebrand factor. Proteolysis by V8 and characterization of two functional domains. 
  • Synaptic vesicle membrane proteins interact to form a multimeric complex. 
  • Synthesis of (3H)DNA complementary to ovalbumin messenger RNA: evidence for limited copies of the ovalbumin gene in chick oviduct. 
  • Synthesis of alpha 2-macroglobulin by bovine adrenal cortical cell cultures. 
  • Tetramerization and single-stranded DNA binding properties of native and mutated forms of murine mitochondrial single-stranded DNA-binding proteins. 
  • The effect of protein synthesis inhibition on the entry of messenger RNA into the cytoplasm of sea urchin embryos. 
  • The role of ribosomal subunits and 80-S monomers in polysome formation in an ascites tumour cell. 
  • Total RNA isolation by a rapid centrifugation method. 
  • Ultrastructure and function of the fractalkine mucin domain in CX(3)C chemokine domain presentation. 
  • Uptake, storage, and distribution of amines in bovine adrenal medullary vesicles. 
  • Use of Sepharose 4B for preparative scale fractionation of eukaryotic messenger RNA's. 
  • Virus-induced genitourinary malignancy. Macromolecular identity. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology