Chromatography

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  Subject Areas on Research

  • 7-Nitrobenz-2-oxa-1,3-diazole (NBD)--phallacidin: synthesis of a fluorescent actin probe. 
  • A human mononuclear leukocyte chemotactic factor: characterization, specificity and kinetics of production by homologous leukocytes. 
  • A monoclonal antibody specific for the amino terminal sequence of human prorenin identifies a common epitope on renal and amniotic fluid inactive renins. 
  • A neutrophil chemotatic factor derived from C'5 upon interaction of guinea pig serum with endotoxin. 
  • Active-site heterogeneity in Limulus hemocyanin as revealed by reaction with peroxides. 
  • An iron tetrahydroporphyrin prosthetic group common to both assimilatory and dissimilatory sulfite reductases. 
  • Antibody-dependent enhancement of human immunodeficiency virus type 1 infection. 
  • Biochemical changes in rat testis during postnatal maturation. 
  • Bone sialoprotein-collagen interaction promotes hydroxyapatite nucleation. 
  • Cathepsin D of rabbit skin: an immunoenzymic study. 
  • Cerebral physiology in dogs after brain-tumor induction with Schmidt-Ruppin Rous sarcoma virus: experiences with radioiodinated normal and antiviral immunoglobulin G. 
  • Collagen, collagenase and clinicians. 
  • Comparative analysis of two different amide-to-ester bond mutations in the beta-sheet of 4-oxalocrotonate tautomerase. 
  • Control of proteoglycan synthesis. Studies on the activation of synthesis observed during culture of articular cartilages. 
  • Degradation of collagen by a human granulocyte collagenolytic system. 
  • Deoxyribonucleic acid ligase. Isolation and physical characterization of the homogeneous enzyme from Escherichia coli. 
  • Distinct patterns of glycosylation of colligin, a collagen-binding glycoprotein, and SPARC (osteonectin), a secreted Ca2+-binding glycoprotein. Evidence for the localisation of colligin in the endoplasmic reticulum. 
  • Effect of hypervitaminosis A on intestinal lipids of rats. 
  • Elucidating the Molecular Composition of Cartilage by Proteomics. 
  • Estimation of liver and intestine lipids of rat by silicic acid column chromatography. 
  • Evaluation of a rapid immunochromatographic test for detection of Streptococcus pneumoniae antigen in urine samples from adults with community-acquired pneumonia. 
  • Expression of cDNAs for G proteins in Escherichia coli. Two forms of Gs alpha stimulate adenylate cyclase. 
  • Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. 
  • Fractionation of transcription factors for RNA polymerase II from Drosophila Kc cell nuclear extracts. 
  • Gene A of X174. 1. Isolation and identification of its products. 
  • Gestational exposure to chlorpyrifos: comparative distribution of trichloropyridinol in the fetus and dam. 
  • High purity isolation and quantification of semiconducting carbon nanotubes via column chromatography. 
  • Human very low density lipoprotein structure: interaction of the C apolipoproteins with apolipoprotein B-100. 
  • Humoral mediators of chemotaxis of mononuclear leukocytes. 
  • Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets. 
  • Identification, partial purification, and characterization of a novel phospholipid-dependent and fatty acid-activated protein kinase from human platelets. 
  • Interactions of the complement system with endotoxic lipopolysaccharide. Generation of a factor chemotactic for polymorphonuclear leukocytes. 
  • Isolation and characterization of the Escherichia coli mutL gene product. 
  • Isolation of a new RNA polymerase-binding protein from sporulating Bacillus subtilis. 
  • Isolation of an ankyrin-band 3 oligomer from human erythrocyte membranes. 
  • Isolation of lysophosphatidylethanolamine from human serum. 
  • Large scale purification of factor X by hydrophobic chromatography. 
  • Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures. 
  • Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. 
  • Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis. 
  • Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. 
  • Membrane receptors as general markers for plasma membrane isolation procedures. The use of 125-I-labeled wheat germ agglutinin, insulin, and cholera toxin. 
  • Metabolism of the aspartyl moiety of N-acetyl-L-aspartic acid in the rat brain. 
  • Norethindrone plasma levels in human subjects. 
  • Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. 
  • Phosphorylation analysis of G protein-coupled receptor by mass spectrometry: identification of a phosphorylation site in V2 vasopressin receptor. 
  • Polyamine stimulation of protein phosphatase-2A from rat liver using a non-protein phosphoester substrate. 
  • Proteomic analysis of arginine adducts on glyoxal-modified ribonuclease. 
  • Purification and biochemical characterization of statin, a nonproliferation specific protein from rat liver. 
  • Purification of recombinant human precursor acid alpha-glucosidase. 
  • Purification of serine-specific transfer ribonucleic acid from bakers' yeast. 
  • R73A and H144Q mutants of the yeast mitochondrial cyclophilin Cpr3 exhibit a low prolyl isomerase activity in both peptide and protein-folding assays. 
  • Rabbit and human liver contain a novel pentacyclic triterpene ester with acyl-CoA: cholesterol acyltransferase inhibitory activity. 
  • Regular structures in unit membranes. II. Morphological and biochemical characterization of two water-soluble membrane proteins isolated from the suckling rat ileum. 
  • Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs. 
  • SERUM LIPIDS IN LEPROSY BY SILICIC ACID COLUMN CHROMATOGRAPHY. 
  • Selective regulation of expression of protein kinase C (PKC) isoenzymes in multidrug-resistant MCF-7 cells. Functional significance of enhanced expression of PKC alpha. 
  • Self-cleavable stimulus responsive tags for protein purification without chromatography. 
  • Separation & estimation of human serum lipids by silicic acid column chromatography. 
  • Simultaneous proteomic profiling of four different growth states of human fibroblasts, using amine-reactive isobaric tagging reagents and tandem mass spectrometry. 
  • Some characteristics of mouse immune (y) interferon produced by a T-lymphoma cell line. 
  • Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei. 
  • Structure determination of human and murine beta-defensins reveals structural conservation in the absence of significant sequence similarity. 
  • Substructure of human von Willebrand factor. Proteolysis by V8 and characterization of two functional domains. 
  • Superoxide dismutase from Streptococcus mutans. Isolation and characterization of two forms of the enzyme. 
  • Synthesis of alpha 2-macroglobulin by bovine adrenal cortical cell cultures. 
  • Systemic administration of a bispecific antibody targeting EGFRvIII successfully treats intracerebral glioma. 
  • The diagnosis of Plasmodium falciparum infection using a new antigen detection system. 
  • The isolation of ecdysterone inducible genes by hybridization subtraction chromatography. 
  • The rapid and large scale separation of peritoneal macrophages. 
  • The stoichiometry of trimeric SIV glycoprotein interaction with CD4 differs from that of anti-envelope antibody Fab fragments. 
  • The typing of fetal antigen 2 in human amniotic fluid. 
  • The voltage-dependent anion channel (VDAC) binds tissue-type plasminogen activator and promotes activation of plasminogen on the cell surface. 
  • Use of artificial neural networks for the accurate prediction of peptide liquid chromatography elution times in proteome analyses. 
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  Keywords of People

  • Cianciolo, George James, Associate Professor Emeritus of Pathology, Pathology
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Biomedical Engineering