Chromatography, Ion Exchange

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  Subject Areas on Research

  • A chemotactic factor for mononuclear leukocytes. 
  • A comparison of the structure of hamster pancreatic insulin and insulin extracted from a transplantable hamster islet-cell carcinoma. 
  • A kit method for the high level synthesis of [211At]MABG. 
  • A mass spectrometry-based proteomic approach to study Marek's Disease Virus gene expression. 
  • A modified procedure for fast purification of T7 RNA polymerase. 
  • A new erythrocyte membrane-associated protein with calmodulin binding activity. Identification and purification. 
  • A new purification procedure for bovine milk xanthine oxidase: effect of proteolysis on the subunit structure. 
  • A novel silver-sulfoethyl cellulose column for purification of ethynyl steroids from biological fluids. 
  • A phosphotransferase that generates phosphatidylinositol 4-phosphate (PtdIns-4-P) from phosphatidylinositol and lipid A in Rhizobium leguminosarum. A membrane-bound enzyme linking lipid a and ptdins-4-p biosynthesis. 
  • A simplified method for isocratic HPLC analysis of polyamines. 
  • A simplified method for the isolation of highly purified bovine retinal S antigen. 
  • Activation of complement by endotoxin. 
  • An active site tyrosine influences the ability of the dimethyl sulfoxide reductase family of molybdopterin enzymes to reduce S-oxides. 
  • An improved method for the isolation of human serum retinol binding protein (S-RBP). 
  • Assay of G-protein beta gamma-subunit complex by catalytic support of ADP-ribosylation of Go alpha. 
  • Assays for the antiangiogenic and neurotrophic serpin pigment epithelium-derived factor. 
  • Both stimulatory and inhibitory GDP/GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins. 
  • CO binding by hemocyanins of Limulus polyphemus, Busycon carica, and Callinectes sapidus. 
  • Characterization of a chimpanzee anti-human melanoma antiserum. 
  • Characterization of corneal proteoglycans under vitamin A deficiency. 
  • Characterization of prorenin activation using a synthetic peptide substrate. 
  • Comparative structural analysis of HLA-A2 antigens distinguishable by cytotoxic T lymphocytes. II. Variant DK1: evidence for a discrete CTL recognition region. 
  • Comparison of the efficacy of IGIV-C, 10% (caprylate/chromatography) and IGIV-SD, 10% as replacement therapy in primary immune deficiency. A randomized double-blind trial. 
  • Conditions for an in vitro culture of murine mixed hematopoietic colonies and their putative cellular origin. 
  • Corneal organ cultures in tyrosinemia release chemotactic factors. 
  • Cumulative impacts of mountaintop mining on an Appalachian watershed. 
  • Cyclic adenosine 3':5'-monophosphate-dependent protein kinase. 
  • DNA polymerase delta is required for human mismatch repair in vitro. 
  • Deoxyribonucleic acid ligase. Isolation and physical characterization of the homogeneous enzyme from Escherichia coli. 
  • Diagnostic challenges of aminoacidopathies and organic acidemias in a developing country: a twelve-year experience. 
  • Dicranin, an antimicrobial and 15-lipoxygenase inhibitor from the moss Dicranum scoparium. 
  • Differential production of high molecular weight sulfated glycoproteins in normal colonic mucosa, primary colon carcinoma, and metastases. 
  • Electron microscopy of MAP 2 (microtubule-associated protein 2). 
  • Evidence against posttranslational glycosylation of rat glucocorticoid receptors. 
  • Functional properties of hemoglobin Pôrto Alegre (alpha2A beta2 9Ser leads to Cys) and the reactivity of its extra cysteinyl residue. 
  • Functional properties of the hemoglobin from the South American snake Mastigodryas bifossatus. 
  • Geochemical evidence for possible natural migration of Marcellus Formation brine to shallow aquifers in Pennsylvania. 
  • Glutathione-associated cis-diamminedichloroplatinum(II) metabolism and ATP-dependent efflux from leukemia cells. Molecular characterization of glutathione-platinum complex and its biological significance. 
  • Guanosinetriphosphatase activity of tubulin associated with microtubule assembly. 
  • Gz, a guanine nucleotide-binding protein with unique biochemical properties. 
  • High affinity of lead for fetal haemoglobin. 
  • Holocarboxylase synthetase deficiency: a biotin-responsive organic acidemia. 
  • Human thymic epithelial cells produce granulocyte and macrophage colony-stimulating factors. 
  • Hyaluronidase activity of alveolar macrophages. 
  • Identification of N-acylphosphatidylserine molecules in eukaryotic cells. 
  • Identification of a cysteine residue essential for activity of protein farnesyltransferase. Cys299 is exposed only upon removal of zinc from the enzyme. 
  • Identification of cellular proteins required for simian virus 40 DNA replication. 
  • Identification of protein phosphatase-1-binding proteins by microcystin-biotin affinity chromatography. 
  • Identification of the endogenous smooth muscle myosin phosphatase-associated kinase. 
  • Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets. 
  • Identification, partial purification, and characterization of a novel phospholipid-dependent and fatty acid-activated protein kinase from human platelets. 
  • Inhibition of purified p21ras farnesyl:protein transferase by Cys-AAX tetrapeptides. 
  • Insulin activates a novel adipocyte mitogen-activated protein kinase kinase kinase that shows rapid phasic kinetics and is distinct from c-Raf. 
  • Isolation and characterization of two group-specific antigens from feline leukemia virus. 
  • Isolation and characterization of viridin, a new 65 kDa antifungal protein from the mould Trichoderma viride. 
  • Isolation and purification of cellular retinol binding protein (CRBP) from goat mammary gland. 
  • Isolation of -L-glutaminyl 4-hydroxybenzene and -L-glutaminyl 3,4-benzoquinone: a natural sulfhydryl reagent, from sporulating gill tissue of the mushroom Agaricus bisporus. 
  • Isolation, purification and characterization of an iron-binding protein from the horseshoe crab (Limulus polyphemus). 
  • Kinetics and mechanism of exogenous anion exchange in FeFbpA-NTA: significance of periplasmic anion lability and anion binding activity of ferric binding protein A. 
  • L-carnitine therapy in isovaleric acidemia. 
  • Large scale purification of factor X by hydrophobic chromatography. 
  • Longitudinal study of differential protein expression in an Alzheimer's mouse model lacking inducible nitric oxide synthase. 
  • Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. 
  • Mammalian protein geranylgeranyltransferase. Subunit composition and metal requirements. 
  • Modulation of neurotoxic esterase activity in vitro by phospholipids. 
  • Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteins. 
  • Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. 
  • Oligonucleotides produced by digestion of bakers' yeast serine transfer ribonucleic acid with specific nucleases. 
  • Optical characterizations and electronic devices of nearly pure (10,5) single-walled carbon nanotubes. 
  • Ordered phosphorylation of p42mapk by MAP kinase kinase. 
  • Overproduction and purification of glutaryl 7-amino cephalosporanic acid acylase. 
  • Overproduction of alpha chains provides a proton-insensitive component to the bluefish hemoglobin system. 
  • Peptide affinity reagents for AAV capsid recognition and purification. 
  • Preparation of high molecular weight invertebrate hemoglobins. 
  • Properties of mouse leukemia viruses. II. Isolation of viral components. 
  • Protein farnesyltransferase and geranylgeranyltransferase share a common alpha subunit. 
  • Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit. 
  • Purification and characterization of a protein associated with peripheral-type benzodiazepine binding sites. 
  • Purification and characterization of one-chain and two-chain renins from mouse submandibular gland. 
  • Purification and partial molecular characterization of GRP94, an ER resident chaperone. 
  • Purification and some properties of Cd-binding protein from rat liver. 
  • Purification of G protein beta gamma from bovine brain. 
  • Purification of a 12,020-dalton protein that enhances the activation of mitogen-activated protein (MAP) kinase by MAP kinase kinase. 
  • Purification of a distinct placental lactogen receptor, a new member of the growth hormone/prolactin receptor family. 
  • Purification of alpha-sarcin and an antifungal protein from Aspergillus giganteus by blue sepharose CL-6B affinity chromatography. 
  • Rapid separation and characterization of protein and peptide mixtures using 1.5 microns diameter non-porous silica in packed capillary liquid chromatography/mass spectrometry. 
  • Regulation of the renal Na(+)-H+ exchanger by calcium calmodulin-dependent multifunctional protein kinase II. 
  • Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs. 
  • S-Nitrosylated fetal hemoglobin in neonatal human blood. 
  • Serum IgD and IgE concentrations in immunodeficiency diseases. 
  • Sialyl-dimeric Lewis-X antigen expressed on mucin-like glycoproteins in colorectal cancer metastases. 
  • Siroheme and sirohydrochlorin. The basis for a new type of porphyrin-related prosthetic group common to both assimilatory and dissimilatory sulfite reductases. 
  • Specific incorporation of molybdopterin in xanthine dehydrogenase of Pseudomonas aeruginosa. 
  • Spectroscopic studies of the molybdenum-containing dimethyl sulfoxide reductase from Rhodobacter sphaeroides f. sp. denitrificans. 
  • Strain variation in composition and molecular size of the capsular polysaccharide of Cryptococcus neoformans serotype A. 
  • Structural analysis of HLA-A2 antigen from immunoselected mutant 8.6.1: further definition of an HLA-A2-specific serological determinant. 
  • Structural analysis of a human glial variant laminin. 
  • Structural analysis of an HLA-B7 antigen variant detected by cytotoxic T lymphocytes. 
  • Tenascin-C is an innate broad-spectrum, HIV-1-neutralizing protein in breast milk. 
  • The kinetics of intramolecular distribution of 15N in uric acid following administration of 15N-glycine: preferential labeling of N-(3+9) of uric acid in primary gout and a reappraisal of the "glutamine hypothesis". 
  • beta-Arrestin: a protein that regulates beta-adrenergic receptor function. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Buckley, Rebecca Hatcher, James Buren Sidbury Distinguished Professor Emeritus of Pediatrics, in the School of Medicine, Pediatrics, Allergy and Immunology
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology