Chromatography, Liquid

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  Subject Areas on Research

  • 3-Aminopyrrolidinone farnesyltransferase inhibitors: design of macrocyclic compounds with improved pharmacokinetics and excellent cell potency. 
  • 450K epigenome-wide scan identifies differential DNA methylation in newborns related to maternal smoking during pregnancy. 
  • A Genoproteomic Approach to Detect Peptide Markers of Bacterial Respiratory Pathogens. 
  • A convenient LC-MS method for assessment of glucose kinetics in vivo with D-[13C6]glucose as a tracer. 
  • A straightforward, quantitative ultra-performance liquid chromatography-tandem mass spectrometric method for heparan sulfate, dermatan sulfate and chondroitin sulfate in urine: an improved clinical screening test for the mucopolysaccharidoses. 
  • A strategy for sensitive, large scale quantitative metabolomics. 
  • A validated LC method for imatinib mesylate. 
  • Activation of protein kinase C by oleic acid. Determination and analysis of inhibition by detergent micelles and physiologic membranes: requirement for free oleate. 
  • Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the Framingham Heart Study. 
  • Alterations in metabolic pathways and networks in Alzheimer's disease. 
  • Alterations in tryptophan and purine metabolism in cocaine addiction: a metabolomic study. 
  • An improved method for glycosaminoglycan analysis by LC-MS/MS of urine samples collected on filter paper. 
  • Analysis of the flame retardant metabolites bis(1,3-dichloro-2-propyl) phosphate (BDCPP) and diphenyl phosphate (DPP) in urine using liquid chromatography-tandem mass spectrometry. 
  • Analysis of thyroid hormones in serum by liquid chromatography-tandem mass spectrometry. 
  • Analysis of ubiquinones, dolichols, and dolichol diphosphate-oligosaccharides by liquid chromatography-electrospray ionization-mass spectrometry. 
  • ApolipoproteinE mimetic peptides improve outcome after focal ischemia. 
  • Application of a label-free, gel-free quantitative proteomics method for ecotoxicological studies of small fish species. 
  • Application of continuous-flow liquid chromatography/fast-atom bombardment mass spectrometry to the analysis of diagnostic acylcarnitines in human urine. 
  • Application of fast atom bombardment with tandem mass spectrometry and liquid chromatography/mass spectrometry to the analysis of acylcarnitines in human urine, blood, and tissue. 
  • Automated, reproducible, titania-based phosphopeptide enrichment strategy for label-free quantitative phosphoproteomics. 
  • Bioavailability of metalloporphyrin-based SOD mimics is greatly influenced by a single charge residing on a Mn site. 
  • Biosynthesis of undecaprenyl phosphate-galactosamine and undecaprenyl phosphate-glucose in Francisella novicida. 
  • Catabolism of 4-hydroxyacids and 4-hydroxynonenal via 4-hydroxy-4-phosphoacyl-CoAs. 
  • Cerebrospinal fluid proteomics in children during induction for acute lymphoblastic leukemia: A pilot study. 
  • Characterization of host-cell line specific glycosylation profiles of early transmitted/founder HIV-1 gp120 envelope proteins. 
  • Characterization of serum proteins associated with IL28B genotype among patients with chronic hepatitis C. 
  • Characterization of the Saccharomyces cerevisiae ATP-Interactome using the iTRAQ-SPROX Technique. 
  • Circulating Autoantibodies in Age-Related Macular Degeneration Recognize Human Macular Tissue Antigens Implicated in Autophagy, Immunomodulation, and Protection from Oxidative Stress and Apoptosis. 
  • Comparative proteomic characterization of articular cartilage tissue from normal donors and patients with osteoarthritis. 
  • Comparing metabolomic and pathologic biomarkers alone and in combination for discriminating Alzheimer's disease from normal cognitive aging. 
  • Comprehensive Proteomic and Metabolomic Signatures of Nontypeable Haemophilus influenzae-Induced Acute Otitis Media Reveal Bacterial Aerobic Respiration in an Immunosuppressed Environment. 
  • Comprehensive pharmacokinetic studies and oral bioavailability of two Mn porphyrin-based SOD mimics, MnTE-2-PyP5+ and MnTnHex-2-PyP5+. 
  • Control of cholecystokinin receptor dephosphorylation in pancreatic acinar cells. 
  • Coordination of platinum therapeutic agents to met-rich motifs of human copper transport protein1. 
  • Design and application of a data-independent precursor and product ion repository. 
  • Development and utilization of a combined LC-UV and LC-MS/MS method for the simultaneous analysis of tegafur and 5-fluorouracil in human plasma to support a phase I clinical study of oral UFT®/leucovorin. 
  • Development and validation of a liquid chromatographic method for the determination of the related substances of ramipril in Altace capsules. 
  • Development of a liquid chromatography-tandem mass spectrometry assay of six antimicrobials in plasma for pharmacokinetic studies in premature infants. 
  • Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS). 
  • Discovering novel brain lipids by liquid chromatography/tandem mass spectrometry. 
  • Discovery of 40 Classes of Per- and Polyfluoroalkyl Substances in Historical Aqueous Film-Forming Foams (AFFFs) and AFFF-Impacted Groundwater. 
  • Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. 
  • Effect of apixaban, an oral and direct factor Xa inhibitor, on coagulation activity biomarkers following acute coronary syndrome. 
  • Efficient analysis of urinary glycosaminoglycans by LC-MS/MS in mucopolysaccharidoses type I, II and VI. 
  • Electrochemical detection of cobalt in hair following on-column derivatization with 1-(2-pyridylazo)-2-naphthol-6-sulphonic acid and reversed-phase liquid chromatography. 
  • Ethanol metabolism modifies hepatic protein acylation in mice. 
  • Extraction parameters for metabolomics from cultured cells. 
  • Fatty acid amide hydrolase inhibition heightens anandamide signaling without producing reinforcing effects in primates. 
  • Fluconazole loading dose pharmacokinetics and safety in infants. 
  • GRP94-associated enzymatic activities. Resolution by chromatographic fractionation. 
  • Generating mammalian sirtuin tools for protein-interaction analysis. 
  • Genotoxicity in Atlantic killifish (Fundulus heteroclitus) from a PAH-contaminated Superfund site on the Elizabeth River, Virginia. 
  • Glutathionylated 4-hydroxy-2-(E)-alkenal enantiomers in rat organs and their contributions toward the disposal of 4-hydroxy-2-(E)-nonenal in rat liver. 
  • Halogenated phenolic contaminants inhibit the in vitro activity of the thyroid-regulating deiodinases in human liver. 
  • Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials. 
  • Identification and Quantitation of Coding Variants and Isoforms of Pulmonary Surfactant Protein A. 
  • Identification of N-acylphosphatidylserine molecules in eukaryotic cells. 
  • Identification of Protease Specificity by Combining Proteome-Derived Peptide Libraries and Quantitative Proteomics. 
  • Identification of S-nitrosylated targets of thioredoxin using a quantitative proteomic approach. 
  • Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium. 
  • Identification of deacetylase substrates with the biotin switch approach. 
  • Identification of dengue RNA binding proteins using RNA chromatography and quantitative mass spectrometry. 
  • Identification of novel quaternary domain interactions in the Hsp90 chaperone, GRP94. 
  • Individual responses to chemotherapy-induced oxidative stress. 
  • Investigating a novel flame retardant known as V6: measurements in baby products, house dust, and car dust. 
  • Ion mobility-derived collision cross section as an additional measure for lipid fingerprinting and identification. 
  • Iron chelation equilibria, redox, and siderophore activity of a saccharide platform ferrichrome analogue. 
  • Isolation of the 36-kD German (Blattella germanica) cockroach allergen using fast protein liquid chromatography. 
  • LC-UV/MS methods for the analysis of prochelator-boronyl salicylaldehyde isonicotinoyl hydrazone (BSIH) and its active chelator salicylaldehyde isonicotinoyl hydrazone (SIH). 
  • Liquid chromatographic assay for a glucose tetrasaccharide, a putative biomarker for the diagnosis of Pompe disease. 
  • Liquid chromatography/tandem mass spectrometry of dolichols and polyprenols, lipid sugar carriers across evolution. 
  • Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins. 
  • Lymphocyte Galactocerebrosidase Activity by LC-MS/MS for Post-Newborn Screening Evaluation of Krabbe Disease. 
  • Mass spectrometry- and lysine amidination-based protocol for thermodynamic analysis of protein folding and ligand binding interactions. 
  • Mass spectrometry-based thermal shift assay for protein-ligand binding analysis. 
  • Measuring fecal testosterone in females and fecal estrogens in males: comparison of RIA and LC/MS/MS methods for wild baboons (Papio cynocephalus). 
  • Metabolomic profiling for the identification of novel biomarkers and mechanisms related to common cardiovascular diseases: form and function. 
  • Metabolomic profiling reveals mitochondrial-derived lipid biomarkers that drive obesity-associated inflammation. 
  • Microproteomics: quantitative proteomic profiling of small numbers of laser-captured cells. 
  • Microvolume trace environmental analysis using peak-focusing online solid-phase extraction-nano-liquid chromatography-high-resolution mass spectrometry. 
  • Mini test dose of intravenous busulfan (busulfex(®)) in allogeneic non-myeloablative stem cell transplantation, followed by liquid chromatography tandem-mass spectrometry. 
  • Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates. 
  • Multiple Ca(2+)-calmodulin-dependent protein kinase kinases from rat brain. Purification, regulation by Ca(2+)-calmodulin, and partial amino acid sequence. 
  • Mutants resistant to LpxC inhibitors by rebalancing cellular homeostasis. 
  • Nitric oxide-mediated regulation of β-amyloid clearance via alterations of MMP-9/TIMP-1. 
  • Novel approach in LC-MS/MS using MRM to generate a full profile of acyl-CoAs: discovery of acyl-dephospho-CoAs. 
  • Oligomeric structure and tissue distribution of ficolins from mouse, pig and human. 
  • Pretreatment metabotype as a predictor of response to sertraline or placebo in depressed outpatients: a proof of concept. 
  • Proteins of purified Epstein-Barr virus. 
  • Proteome-wide muscle protein fractional synthesis rates predict muscle mass gain in response to a selective androgen receptor modulator in rats. 
  • Proteomic analysis of an unculturable bacterial endosymbiont (Blochmannia) reveals high abundance of chaperonins and biosynthetic enzymes. 
  • Proteomic analysis of stage I endometrial cancer tissue: identification of proteins associated with oxidative processes and inflammation. 
  • Proteomic differences between male and female anterior cruciate ligament and patellar tendon. 
  • Quantification and Potential Functions of Endogenous Agonists of Transient Receptor Potential Channels in Patients With Irritable Bowel Syndrome. 
  • Quantification of glycosaminoglycans in urine by isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry. 
  • Quantitative proteomics approach for identifying protein-drug interactions in complex mixtures using protein stability measurements. 
  • Quantitative proteomics reveals metabolic and pathogenic properties of Chlamydia trachomatis developmental forms. 
  • Rapid method for the measurement of circulating thyroid hormones in low volumes of teleost fish plasma by LC-ESI/MS/MS. 
  • Receptor-specific desensitization with purified proteins. Kinase dependence and receptor specificity of beta-arrestin and arrestin in the beta 2-adrenergic receptor and rhodopsin systems. 
  • Selective detection of monohydroxy metabolites of polycyclic aromatic hydrocarbons in urine using liquid chromatography/triple quadrupole tandem mass spectrometry. 
  • Simultaneous proteomic profiling of four different growth states of human fibroblasts, using amine-reactive isobaric tagging reagents and tandem mass spectrometry. 
  • Sphingolipidomics: high-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry. 
  • Stability-indicating LC assay of and impurity identification in homoharringtonine samples. 
  • Structure-specific, quantitative methods for analysis of sphingolipids by liquid chromatography-tandem mass spectrometry: "inside-out" sphingolipidomics. 
  • Systemic oxidative stress, as measured by urinary allantoin and F(2)-isoprostanes, is not increased in Down syndrome. 
  • Targeted Proteomics of Human Metapneumovirus in Clinical Samples and Viral Cultures. 
  • The Chlamydia trachomatis type III secretion chaperone Slc1 engages multiple early effectors, including TepP, a tyrosine-phosphorylated protein required for the recruitment of CrkI-II to nascent inclusions and innate immune signaling. 
  • The antigen for Hep Par 1 antibody is the urea cycle enzyme carbamoyl phosphate synthetase 1. 
  • The cellular lipids of Romboutsia. 
  • The chemokines IL-8, monocyte chemoattractant protein-1, and I-309 are monomers at physiologically relevant concentrations. 
  • The effect of Rho-associated kinase inhibition on the ocular penetration of timolol maleate. 
  • The intestinal glucose-apelin cycle controls carbohydrate absorption in mice. 
  • The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile. 
  • The protein expression landscape of the Arabidopsis root. 
  • Thermal stability of bimatoprost, latanoprost, and travoprost under simulated daily use. 
  • Total body skeletal muscle mass: estimation by creatine (methyl-d3) dilution in humans. 
  • Total synthesis, purification, and characterization of human [Phe(p-CH2SO 3Na)52, Nle32,53,56, Nal55]-CCK20-58, [Tyr52, Nle32,53,56, Nal55]-CCK-58, and [Phe(p-CH2SO3Na)52, Nle32,53,56, Nal55]-CCK-58. 
  • Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. 
  • Urinary F2-isoprostanes, obesity, and weight gain in the IRAS cohort. 
  • Urinary biomarkers of oxidative status in a clinical model of oxidative assault. 
  • Urinary metabolites of organophosphate flame retardants and their variability in pregnant women. 
  • Vitamin D deficiency in neovascular versus nonneovascular age-related macular degeneration. 
  • pH stability of individual folates during critical sample preparation steps in prevision of the analysis of plant folates. 
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  Keywords of People

  • Alexander, John Hunter Peel, Professor of Medicine, Medicine, Cardiology
  • Berchuck, Andrew, James M. Ingram Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Cohen-Wolkowiez, Michael, Professor of Pediatrics, Pediatrics, Infectious Diseases
  • Erickson, Harold Paul, James B. Duke Professor of Medicine, Cell Biology
  • Guan, Ziqiang, Associate Research Professor in Biochemistry, Biochemistry