Chromatography, Liquid

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  Subject Areas on Research

  • 3-Aminopyrrolidinone farnesyltransferase inhibitors: design of macrocyclic compounds with improved pharmacokinetics and excellent cell potency. 
  • 450K epigenome-wide scan identifies differential DNA methylation in newborns related to maternal smoking during pregnancy. 
  • A Genoproteomic Approach to Detect Peptide Markers of Bacterial Respiratory Pathogens. 
  • A Prospective Study of Urinary Prostaglandin E2 Metabolite, Helicobacter pylori Antibodies, and Gastric Cancer Risk. 
  • A comparative analysis of egg provisioning using mass spectrometry during rapid life history evolution in sea urchins. 
  • A convenient LC-MS method for assessment of glucose kinetics in vivo with D-[13C6]glucose as a tracer. 
  • A draft genome sequence of the elusive giant squid, Architeuthis dux. 
  • A novel interface for variable flow nanoscale LC/MS/MS for improved proteome coverage. 
  • A phase II study of the Ras-MAPK signaling pathway inhibitor TLN-4601 in patients with glioblastoma at first progression. 
  • A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani. 
  • A predicted geranylgeranyl reductase reduces the ω-position isoprene of dolichol phosphate in the halophilic archaeon, Haloferax volcanii. 
  • A proteomics approach to the identification of mammalian mitochondrial small subunit ribosomal proteins. 
  • A straightforward, quantitative ultra-performance liquid chromatography-tandem mass spectrometric method for heparan sulfate, dermatan sulfate and chondroitin sulfate in urine: an improved clinical screening test for the mucopolysaccharidoses. 
  • A strategy for sensitive, large scale quantitative metabolomics. 
  • A validated LC method for imatinib mesylate. 
  • Absorption and tolerability of aqueous chlorhexidine gluconate used for skin antisepsis prior to catheter insertion in preterm neonates. 
  • Activation of protein kinase C by oleic acid. Determination and analysis of inhibition by detergent micelles and physiologic membranes: requirement for free oleate. 
  • Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the Framingham Heart Study. 
  • AglR is required for addition of the final mannose residue of the N-linked glycan decorating the Haloferax volcanii S-layer glycoprotein. 
  • Alterations in metabolic pathways and networks in Alzheimer's disease. 
  • Alterations in tryptophan and purine metabolism in cocaine addiction: a metabolomic study. 
  • An ethanolamine-phosphate modified glycolipid in Clostridium acetobutylicum that responds to membrane stress. 
  • An improved method for glycosaminoglycan analysis by LC-MS/MS of urine samples collected on filter paper. 
  • Analysis of the flame retardant metabolites bis(1,3-dichloro-2-propyl) phosphate (BDCPP) and diphenyl phosphate (DPP) in urine using liquid chromatography-tandem mass spectrometry. 
  • Analysis of thyroid hormones in serum by liquid chromatography-tandem mass spectrometry. 
  • Analysis of ubiquinones, dolichols, and dolichol diphosphate-oligosaccharides by liquid chromatography-electrospray ionization-mass spectrometry. 
  • Analytical and biological methods for probing the blood-brain barrier. 
  • ApolipoproteinE mimetic peptides improve outcome after focal ischemia. 
  • Application of a label-free, gel-free quantitative proteomics method for ecotoxicological studies of small fish species. 
  • Application of an LC-MS/MS method for reliable determination of amodiaquine, N-desethylamodiaquine, artesunate and dihydroartemisinin in human plasma for a bioequivalence study in healthy Indian subjects. 
  • Application of continuous-flow liquid chromatography/fast-atom bombardment mass spectrometry to the analysis of diagnostic acylcarnitines in human urine. 
  • Application of fast atom bombardment with tandem mass spectrometry and liquid chromatography/mass spectrometry to the analysis of acylcarnitines in human urine, blood, and tissue. 
  • Application of proteomic marker ensembles to subcellular organelle identification. 
  • Association between plasma BPDE-Alb adduct concentrations and DNA damage of peripheral blood lymphocytes among coke oven workers. 
  • Automated, reproducible, titania-based phosphopeptide enrichment strategy for label-free quantitative phosphoproteomics. 
  • Bioavailability of metalloporphyrin-based SOD mimics is greatly influenced by a single charge residing on a Mn site. 
  • Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773. 
  • Biosynthesis of undecaprenyl phosphate-galactosamine and undecaprenyl phosphate-glucose in Francisella novicida. 
  • Cardiovascular Metabolomics. 
  • Catabolism of 4-hydroxyacids and 4-hydroxynonenal via 4-hydroxy-4-phosphoacyl-CoAs. 
  • Cerebrospinal fluid proteomics in children during induction for acute lymphoblastic leukemia: A pilot study. 
  • Characterization of host-cell line specific glycosylation profiles of early transmitted/founder HIV-1 gp120 envelope proteins. 
  • Characterization of serum proteins associated with IL28B genotype among patients with chronic hepatitis C. 
  • Characterization of the Saccharomyces cerevisiae ATP-Interactome using the iTRAQ-SPROX Technique. 
  • Characterization of the ubiquitin-modified proteome regulated by transient forebrain ischemia. 
  • Chemo-Selection Strategy for Limited Proteolysis Experiments on the Proteomic Scale. 
  • Circulating Autoantibodies in Age-Related Macular Degeneration Recognize Human Macular Tissue Antigens Implicated in Autophagy, Immunomodulation, and Protection from Oxidative Stress and Apoptosis. 
  • Closing the Mass Balance on Fluorine on Papers and Textiles. 
  • Comparative Analysis of Ampicillin Plasma and Dried Blood Spot Pharmacokinetics in Neonates. 
  • Comparative proteomic characterization of articular cartilage tissue from normal donors and patients with osteoarthritis. 
  • Comparing metabolomic and pathologic biomarkers alone and in combination for discriminating Alzheimer's disease from normal cognitive aging. 
  • Comprehensive Proteomic and Metabolomic Signatures of Nontypeable Haemophilus influenzae-Induced Acute Otitis Media Reveal Bacterial Aerobic Respiration in an Immunosuppressed Environment. 
  • Comprehensive pharmacokinetic studies and oral bioavailability of two Mn porphyrin-based SOD mimics, MnTE-2-PyP5+ and MnTnHex-2-PyP5+. 
  • Control of cholecystokinin receptor dephosphorylation in pancreatic acinar cells. 
  • Coordination of platinum therapeutic agents to met-rich motifs of human copper transport protein1. 
  • Coupling of capillary zone electrophoresis and capillary liquid chromatography with coaxial continuous-flow fast atom bombardment tandem sector mass spectrometry. 
  • Current trends in differential expression proteomics: isotopically coded tags. 
  • DNA-PK facilitates piggyBac transposition by promoting paired-end complex formation. 
  • Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria. 
  • Delivery of antisense oligonucleotides using HPMA polymer: synthesis of A thiol polymer and its conjugation to water-soluble molecules. 
  • Design and application of a data-independent precursor and product ion repository. 
  • Determination of Real Time in Vivo Drug Receptor Occupancy for a Covalent Binding Drug as a Clinical Pharmacodynamic Biomarker by Immunocapture-LC-MS/MS. 
  • Development and utilization of a combined LC-UV and LC-MS/MS method for the simultaneous analysis of tegafur and 5-fluorouracil in human plasma to support a phase I clinical study of oral UFT®/leucovorin. 
  • Development and validation of a high-throughput stereoselective LC-MS/MS assay for bupropion, hydroxybupropion, erythrohydrobupropion, and threohydrobupropion in human plasma. 
  • Development and validation of a liquid chromatographic method for the determination of the related substances of ramipril in Altace capsules. 
  • Development of a liquid chromatography-tandem mass spectrometry assay of six antimicrobials in plasma for pharmacokinetic studies in premature infants. 
  • Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS). 
  • Discovering novel brain lipids by liquid chromatography/tandem mass spectrometry. 
  • Discovery of 40 Classes of Per- and Polyfluoroalkyl Substances in Historical Aqueous Film-Forming Foams (AFFFs) and AFFF-Impacted Groundwater. 
  • Discovery of Lipidome Alterations Following Traumatic Brain Injury via High-Resolution Metabolomics. 
  • Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. 
  • Discovery of biomarker candidates for coronary artery disease from an APOE-knock out mouse model using iTRAQ-based multiplex quantitative proteomics. 
  • Early zebrafish embryogenesis is susceptible to developmental TDCPP exposure. 
  • Effect of apixaban, an oral and direct factor Xa inhibitor, on coagulation activity biomarkers following acute coronary syndrome. 
  • Efficient analysis of urinary glycosaminoglycans by LC-MS/MS in mucopolysaccharidoses type I, II and VI. 
  • Electrochemical detection of cobalt in hair following on-column derivatization with 1-(2-pyridylazo)-2-naphthol-6-sulphonic acid and reversed-phase liquid chromatography. 
  • Ethanol metabolism modifies hepatic protein acylation in mice. 
  • Evidence that Gsta4 modifies susceptibility to skin tumor development in mice and humans. 
  • Exploiting the complementary nature of LC/MALDI/MS/MS and LC/ESI/MS/MS for increased proteome coverage. 
  • Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy. 
  • Exposure to flame retardant chemicals on commercial airplanes. 
  • Extraction parameters for metabolomics from cultured cells. 
  • Fatty acid amide hydrolase inhibition heightens anandamide signaling without producing reinforcing effects in primates. 
  • Fluconazole loading dose pharmacokinetics and safety in infants. 
  • GRP94-associated enzymatic activities. Resolution by chromatographic fractionation. 
  • Generating mammalian sirtuin tools for protein-interaction analysis. 
  • Genotoxicity in Atlantic killifish (Fundulus heteroclitus) from a PAH-contaminated Superfund site on the Elizabeth River, Virginia. 
  • Glutathione S-conjugation of the sevoflurane degradation product, fluoromethyl-2,2-difluoro-1-(trifluoromethyl)vinyl ether (compound A) in human liver, kidney, and blood in vitro. 
  • Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma. 
  • Glutathionylated 4-hydroxy-2-(E)-alkenal enantiomers in rat organs and their contributions toward the disposal of 4-hydroxy-2-(E)-nonenal in rat liver. 
  • Halogenated phenolic contaminants inhibit the in vitro activity of the thyroid-regulating deiodinases in human liver. 
  • High-Intensity Sweeteners in Alternative Tobacco Products. 
  • High-sensitivity analysis of buprenorphine, norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide in plasma and urine by liquid chromatography-mass spectrometry. 
  • Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function. 
  • Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials. 
  • Identification and Quantitation of Coding Variants and Isoforms of Pulmonary Surfactant Protein A. 
  • Identification and characterization of a leucine-rich repeat kinase 2 (LRRK2) consensus phosphorylation motif. 
  • Identification in rat bile of glutathione conjugates of fluoromethyl 2,2-difluoro-1-(trifluoromethyl)vinyl ether, a nephrotoxic degradate of the anesthetic agent sevoflurane. 
  • Identification of N-acylphosphatidylserine molecules in eukaryotic cells. 
  • Identification of Protease Specificity by Combining Proteome-Derived Peptide Libraries and Quantitative Proteomics. 
  • Identification of S-nitrosylated targets of thioredoxin using a quantitative proteomic approach. 
  • Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium. 
  • Identification of deacetylase substrates with the biotin switch approach. 
  • Identification of dengue RNA binding proteins using RNA chromatography and quantitative mass spectrometry. 
  • Identification of impurities in erythromycin by liquid chromatography-mass spectrometric detection. 
  • Identification of novel quaternary domain interactions in the Hsp90 chaperone, GRP94. 
  • In-depth proteomic analysis of nonsmall cell lung cancer to discover molecular targets and candidate biomarkers. 
  • Individual responses to chemotherapy-induced oxidative stress. 
  • Investigating a novel flame retardant known as V6: measurements in baby products, house dust, and car dust. 
  • Ion mobility-derived collision cross section as an additional measure for lipid fingerprinting and identification. 
  • Iron chelation equilibria, redox, and siderophore activity of a saccharide platform ferrichrome analogue. 
  • LC-UV/MS methods for the analysis of prochelator-boronyl salicylaldehyde isonicotinoyl hydrazone (BSIH) and its active chelator salicylaldehyde isonicotinoyl hydrazone (SIH). 
  • Laboratory analysis of amino acids, 2018 revision: a technical standard of the American College of Medical Genetics and Genomics (ACMG). 
  • Lipid modification gives rise to two distinct Haloferax volcanii S-layer glycoprotein populations. 
  • Lipidomic Analysis of Clostridium cadaveris and Clostridium fallax. 
  • Liquid chromatographic assay for a glucose tetrasaccharide, a putative biomarker for the diagnosis of Pompe disease. 
  • Liquid chromatography/tandem mass spectrometry of dolichols and polyprenols, lipid sugar carriers across evolution. 
  • Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins. 
  • Lymphocyte Galactocerebrosidase Activity by LC-MS/MS for Post-Newborn Screening Evaluation of Krabbe Disease. 
  • Macrophage proresolving mediator maresin 1 stimulates tissue regeneration and controls pain. 
  • Manganese porphyrin redox state in endothelial cells: Resonance Raman studies and implications for antioxidant protection towards peroxynitrite. 
  • Mass spectrometry- and lysine amidination-based protocol for thermodynamic analysis of protein folding and ligand binding interactions. 
  • Mass spectrometry-based thermal shift assay for protein-ligand binding analysis. 
  • Measuring fecal testosterone in females and fecal estrogens in males: comparison of RIA and LC/MS/MS methods for wild baboons (Papio cynocephalus). 
  • Meta-analysis of genome-wide association studies for circulating phylloquinone concentrations. 
  • Metabolomic profiling for the identification of novel biomarkers and mechanisms related to common cardiovascular diseases: form and function. 
  • Metabolomic profiling reveals mitochondrial-derived lipid biomarkers that drive obesity-associated inflammation. 
  • Metabolomics: A Primer. 
  • Microproteomics: quantitative proteomic profiling of small numbers of laser-captured cells. 
  • Microvolume trace environmental analysis using peak-focusing online solid-phase extraction-nano-liquid chromatography-high-resolution mass spectrometry. 
  • Mini test dose of intravenous busulfan (busulfex(®)) in allogeneic non-myeloablative stem cell transplantation, followed by liquid chromatography tandem-mass spectrometry. 
  • Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates. 
  • Multiple Ca(2+)-calmodulin-dependent protein kinase kinases from rat brain. Purification, regulation by Ca(2+)-calmodulin, and partial amino acid sequence. 
  • Mutants resistant to LpxC inhibitors by rebalancing cellular homeostasis. 
  • Nanoscale packed-capillary liquid chromatography coupled with mass spectrometry using a coaxial continuous-flow fast atom bombardment interface. 
  • Nitric oxide-mediated regulation of β-amyloid clearance via alterations of MMP-9/TIMP-1. 
  • Non-enzymatically derived minor lipids found in Escherichia coli lipid extracts. 
  • Novel approach in LC-MS/MS using MRM to generate a full profile of acyl-CoAs: discovery of acyl-dephospho-CoAs. 
  • Oligomeric structure and tissue distribution of ficolins from mouse, pig and human. 
  • Opposing effects of cancer-type-specific SPOP mutants on BET protein degradation and sensitivity to BET inhibitors. 
  • Pharmacokinetic Analysis of a Novel Human EGFRvIII:CD3 Bispecific Antibody in Plasma and Whole Blood Using a High-Resolution Targeted Mass Spectrometry Approach. 
  • Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms. 
  • Potent Anti-HIV Ingenane Diterpenoids from Euphorbia ebracteolata. 
  • Preliminary investigation of the use of newborn dried blood spots for screening pyridoxine-dependent epilepsy by LC-MS/MS. 
  • Pretreatment metabotype as a predictor of response to sertraline or placebo in depressed outpatients: a proof of concept. 
  • Proteins of purified Epstein-Barr virus. 
  • Proteome analyses using accurate mass and elution time peptide tags with capillary LC time-of-flight mass spectrometry. 
  • Proteome-wide muscle protein fractional synthesis rates predict muscle mass gain in response to a selective androgen receptor modulator in rats. 
  • Proteomic analysis of an unculturable bacterial endosymbiont (Blochmannia) reveals high abundance of chaperonins and biosynthetic enzymes. 
  • Proteomic analysis of stage I endometrial cancer tissue: identification of proteins associated with oxidative processes and inflammation. 
  • Proteomic differences between male and female anterior cruciate ligament and patellar tendon. 
  • Proteomics characterization of cell membrane blebs in human retinal pigment epithelium cells. 
  • Purification and characterization of zeta-crystallin/quinone reductase from guinea pig liver. 
  • Quantification and Potential Functions of Endogenous Agonists of Transient Receptor Potential Channels in Patients With Irritable Bowel Syndrome. 
  • Quantification of glycosaminoglycans in urine by isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry. 
  • Quantitative proteomics approach for identifying protein-drug interactions in complex mixtures using protein stability measurements. 
  • Quantitative proteomics reveals metabolic and pathogenic properties of Chlamydia trachomatis developmental forms. 
  • RRmix: A method for simultaneous batch effect correction and analysis of metabolomics data in the absence of internal standards. 
  • Race-specific molecular alterations correlate with differential outcomes for black and white endometrioid endometrial cancer patients. 
  • Rapid method for the measurement of circulating thyroid hormones in low volumes of teleost fish plasma by LC-ESI/MS/MS. 
  • Receptor-specific desensitization with purified proteins. Kinase dependence and receptor specificity of beta-arrestin and arrestin in the beta 2-adrenergic receptor and rhodopsin systems. 
  • Reference ranges for testosterone in men generated using liquid chromatography tandem mass spectrometry in a community-based sample of healthy nonobese young men in the Framingham Heart Study and applied to three geographically distinct cohorts. 
  • Scanning Quadrupole Data-Independent Acquisition, Part A: Qualitative and Quantitative Characterization. 
  • Scanning Quadrupole Data-Independent Acquisition, Part B: Application to the Analysis of the Calcineurin-Interacting Proteins during Treatment of Aspergillus fumigatus with Azole and Echinocandin Antifungal Drugs. 
  • Selective detection of monohydroxy metabolites of polycyclic aromatic hydrocarbons in urine using liquid chromatography/triple quadrupole tandem mass spectrometry. 
  • Seroconversion on preexposure prophylaxis: a case report with segmental hair analysis for timed adherence determination. 
  • Sex hormone-binding globulin, but not testosterone, is associated prospectively and independently with incident metabolic syndrome in men: the framingham heart study. 
  • Simultaneous determination of alpha-aminoadipic semialdehyde, piperideine-6-carboxylate and pipecolic acid by LC-MS/MS for pyridoxine-dependent seizures and folinic acid-responsive seizures. 
  • Simultaneous proteomic profiling of four different growth states of human fibroblasts, using amine-reactive isobaric tagging reagents and tandem mass spectrometry. 
  • Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS. 
  • Sphingolipidomics: high-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry. 
  • Stability-indicating LC assay of and impurity identification in homoharringtonine samples. 
  • Stereoselective determination of methadone and the primary metabolite EDDP in human plasma by automated on-line extraction and liquid chromatography mass spectrometry. 
  • Strobilurin fungicides in house dust: is wallboard a source? 
  • Structure-specific, quantitative methods for analysis of sphingolipids by liquid chromatography-tandem mass spectrometry: "inside-out" sphingolipidomics. 
  • Systemic exposure to fluticasone MDI delivered through antistatic chambers. 
  • Systemic oxidative stress, as measured by urinary allantoin and F(2)-isoprostanes, is not increased in Down syndrome. 
  • Targeted Proteomics of Human Metapneumovirus in Clinical Samples and Viral Cultures. 
  • The 2012/2013 ABRF Proteomic Research Group Study: Assessing Longitudinal Intralaboratory Variability in Routine Peptide Liquid Chromatography Tandem Mass Spectrometry Analyses. 
  • The Chlamydia trachomatis type III secretion chaperone Slc1 engages multiple early effectors, including TepP, a tyrosine-phosphorylated protein required for the recruitment of CrkI-II to nascent inclusions and innate immune signaling. 
  • The antigen for Hep Par 1 antibody is the urea cycle enzyme carbamoyl phosphate synthetase 1. 
  • The cellular lipids of Romboutsia. 
  • The chemokines IL-8, monocyte chemoattractant protein-1, and I-309 are monomers at physiologically relevant concentrations. 
  • The effect of Rho-associated kinase inhibition on the ocular penetration of timolol maleate. 
  • The heritability of circulating testosterone, oestradiol, oestrone and sex hormone binding globulin concentrations in men: the Framingham Heart Study. 
  • The intestinal glucose-apelin cycle controls carbohydrate absorption in mice. 
  • The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile. 
  • The protein expression landscape of the Arabidopsis root. 
  • The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates. 
  • The unanticipated loss of SO2 from sulfonamides in collision-induced dissociation. 
  • Thermal stability of bimatoprost, latanoprost, and travoprost under simulated daily use. 
  • Total body skeletal muscle mass: estimation by creatine (methyl-d3) dilution in humans. 
  • Total synthesis, purification, and characterization of human [Phe(p-CH2SO 3Na)52, Nle32,53,56, Nal55]-CCK20-58, [Tyr52, Nle32,53,56, Nal55]-CCK-58, and [Phe(p-CH2SO3Na)52, Nle32,53,56, Nal55]-CCK-58. 
  • Total testosterone assays in women with polycystic ovary syndrome: precision and correlation with hirsutism. 
  • Towards establishing indicative values for metabolites of organophosphate ester contaminants in human urine. 
  • Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. 
  • Understanding the Role of Aggregation in the Broad Absorption Bands of Eumelanin 
  • Urinary F2-isoprostanes, obesity, and weight gain in the IRAS cohort. 
  • Urinary biomarkers of oxidative status in a clinical model of oxidative assault. 
  • Urinary metabolites of organophosphate flame retardants and their variability in pregnant women. 
  • Very high pressure gradient LC/MS/MS. 
  • Vitamin D deficiency in neovascular versus nonneovascular age-related macular degeneration. 
  • XPO1/CRM1 Inhibition Causes Antitumor Effects by Mitochondrial Accumulation of eIF5A. 
  • pH stability of individual folates during critical sample preparation steps in prevision of the analysis of plant folates. 
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  Keywords of People

  • Alexander, John Hunter Peel, Professor of Medicine, Medicine, Cardiology
  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Cohen-Wolkowiez, Michael, Professor of Pediatrics, Pediatrics, Infectious Diseases
  • Erickson, Harold Paul, James B. Duke Distinguished Professor of Cell Biology, Cell Biology
  • Guan, Ziqiang, Research Professor in Biochemistry, Biochemistry