Circular Dichroism

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  Subject Areas on Research

  • "Dynamic Infrared Linear Dichroism Study of the Temperature-Dependent, Viscoelastic Behavior of a Poly(ester urethane)" 
  • (i, i + 4) Ion pairs stabilize helical peptides derived from smooth muscle caldesmon. 
  • 440-kD ankyrinB: structure of the major developmentally regulated domain and selective localization in unmyelinated axons. 
  • A carbon monoxide irreducible form of cytochrome c oxidase and other unusual properties of the "monomeric" shark enzyme. 
  • A carcinoembryonic antigen (CEA) binding protein from ascites influences CEA uptake by macrophages. 
  • A molybdopterin-free form of xanthine oxidase. 
  • A mutation in TNNC1-encoded cardiac troponin C, TNNC1-A31S, predisposes to hypertrophic cardiomyopathy and ventricular fibrillation. 
  • A stable dimer in the pH-induced equilibrium unfolding of the homo-hexameric enzyme 4-oxalocrotonate tautomerase (4-OT). 
  • A synthetic peptide inhibitor of human immunodeficiency virus replication: correlation between solution structure and viral inhibition. 
  • Amphitrite ornata erythrocruorin. I. Structural properties and characterization of subunit interactions. 
  • Analysis of the structural properties of cAMP-responsive element-binding protein (CREB) and phosphorylated CREB. 
  • Antigenicity and immunogenicity of a trimeric envelope protein from an Indian clade C HIV-1 isolate. 
  • Apolipoprotein C-III/sphingomyelin recombinants: formation, isolation, and characterization. 
  • Asymmetric total synthesis of ent-(-)-roseophilin: assignment of absolute configuration. 
  • Betadoublet: de novo design, synthesis, and characterization of a beta-sandwich protein. 
  • Bioactive ent-isopimarane diterpenoids from Euphorbia neriifolia. 
  • Biochemical and structural studies of tenascin/hexabrachion proteins. 
  • Boron-containing oligodeoxyribonucleotide 14mer duplexes: enzymatic synthesis and melting studies. 
  • Both heptad repeats of human respiratory syncytial virus fusion protein are potent inhibitors of viral fusion. 
  • Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues. 
  • Carolignans from the Aerial Parts of Euphorbia sikkimensis and Their Anti-HIV Activity. 
  • Chaperone-like activity of alpha-crystallin. The effect of NADPH on its interaction with zeta-crystallin. 
  • Characterization of the SarA virulence gene regulator of Staphylococcus aureus. 
  • Characterization of the adhesive plaque of the barnacle Balanus amphitrite: amyloid-like nanofibrils are a major component. 
  • Characterization of the ionizable groups interacting with anionic allosteric effectors of human hemoglobin. 
  • Chemical and structural modifications of alpha 2-macroglobulin: effects on receptor binding and endocytosis studied in an in vivo model. 
  • Chemical modification and fluorescence studies of chloroplast coupling factor. 
  • Chiral polyoxometalate-induced enantiomerically 3D architectures: a new route for synthesis of high-dimensional chiral compounds. 
  • Circular dichroic spectroscopy of non-human alpha-macroglobulins. 
  • Co-assembling peptides as defined matrices for endothelial cells. 
  • Comparative analysis of two different amide-to-ester bond mutations in the beta-sheet of 4-oxalocrotonate tautomerase. 
  • Comparative studies on the secondary structure of ovalbumin messenger RNA and its complementary DNA transcript. 
  • Computational de novo design and characterization of a protein that selectively binds a highly hyperpolarizable abiological chromophore. 
  • Computational design and elaboration of a de novo heterotetrameric alpha-helical protein that selectively binds an emissive abiological (porphinato)zinc chromophore. 
  • Conformational changes of beta-lactoglobulin induced by anionic phospholipid. 
  • Conformational dynamics of amyloid beta-protein assembly probed using intrinsic fluorescence. 
  • Conformational studies of dithymidine boranomonophosphate diastereoisomers. 
  • Conformational studies of the nucleic acid binding sites for Xenopus transcription factor IIIA. 
  • Conformational transition of DNA induced by cationic lipid vesicle in acidic solution: spectroscopy investigation. 
  • Construction of a family of Cys2His2 zinc binding sites in the hydrophobic core of thioredoxin by structure-based design. 
  • Construction of new ligand binding sites in proteins of known structure. II. Grafting of a buried transition metal binding site into Escherichia coli thioredoxin. 
  • Converting a maltose receptor into a nascent binuclear copper oxygenase by computational design. 
  • Coordination chemistry of siderophores: thermodynamics and kinetics of iron chelation and release. 
  • Crystal structures of apocalmodulin and an apocalmodulin/SK potassium channel gating domain complex. 
  • De novo design and molecular assembly of a transmembrane diporphyrin-binding protein complex. 
  • De novo design of a single-chain diphenylporphyrin metalloprotein. 
  • Determining absolute configuration in flexible molecules: a case study. 
  • Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha. 
  • Disruption of the toxic conformation of the expanded polyglutamine stretch leads to suppression of aggregate formation and cytotoxicity. 
  • Domain interactions in oxidized and reduced forms of rat liver sulfite oxidase. 
  • Downregulation of microglial activation by apolipoprotein E and apoE-mimetic peptides. 
  • Effect of acid predissolution on fibril size and fibril flexibility of synthetic beta-amyloid peptide. 
  • Effect of colloidal gold size on the conformational changes of adsorbed cytochrome c: probing by circular dichroism, UV-visible, and infrared spectroscopy. 
  • Effect of detergents on the thermal behavior of elastin-like polypeptides. 
  • Effect of electrode surface microstructure on electron transfer induced conformation changes in cytochrome c monitored by in situ UV and CD spectroelectrochemistry. 
  • Effect of temperature, pH, and metals on the stability and activity of phenylalanine hydroxylase from Chromobacterium violaceum. 
  • Electrochemistry and spectroscopy study on the interaction of microperoxidase-11 with lipid membrane. 
  • Electron microscopy of MAP 2 (microtubule-associated protein 2). 
  • Escherichia coli purine repressor: key residues for the allosteric transition between active and inactive conformations and for interdomain signaling. 
  • Evaluation of the DNA binding tendencies of the transition state regulator AbrB. 
  • Evidence against formation of A23187 dimers and oligomers in solution: photo-induced degradation of Ionophore A23187. 
  • Evidence of Fe3+ interaction with the plug domain of the outer membrane transferrin receptor protein of Neisseria gonorrhoeae: implications for Fe transport. 
  • Expression and site-directed mutagenesis of the phosphatidylcholine-preferring phospholipase C of Bacillus cereus: probing the role of the active site Glu146. 
  • Facile chemical synthesis and equilibrium unfolding properties of CopG. 
  • Fluorescence energy transfer measurements in rabbit muscle phosphofructokinase. 
  • Folding dynamics of phenylalanine hydroxylase depends on the enzyme's metallation state: the native metal, iron, protects against aggregate intermediates. 
  • Folding kinetics of a fluorescent variant of monomeric lambda repressor. 
  • Folding mechanism of a multiple independently-folding domain protein: double B domain of protein A. 
  • Functionally active targeting domain of the beta-adrenergic receptor kinase: an inhibitor of G beta gamma-mediated stimulation of type II adenylyl cyclase. 
  • G4 resolvase 1 tightly binds and unwinds unimolecular G4-DNA. 
  • Genetic organization, length conservation, and evolution of RNA polymerase II carboxyl-terminal domain. 
  • Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. 
  • Guanidine-induced equilibrium unfolding of a homo-hexameric enzyme 4-oxalocrotonate tautomerase (4-OT). 
  • Heat and cold denatured states of monomeric lambda repressor are thermodynamically and conformationally equivalent. 
  • Hormonal control of oviduct growth and differentiation. 
  • How can a single second sphere amino acid substitution cause reduction midpoint potential changes of hundreds of millivolts? 
  • Hydrogen bonding of beta-turn structure is stabilized in D(2)O. 
  • Immunoglobulin-like PapD chaperone caps and uncaps interactive surfaces of nascently translocated pilus subunits. 
  • Incorporation of CC steps into Z-DNA: interplay between B-Z junction and Z-DNA helical formation. 
  • Incorporation of designed extended chromophores into amphiphilic 4-helix bundle peptides for nonlinear optical biomolecular materials. 
  • Interaction of gold nanoparticles with common human blood proteins. 
  • Interaction of synthetic HA2 influenza fusion peptide analog with model membranes. 
  • Interaction of the Shiga-like toxin type 1 B-subunit with its carbohydrate receptor. 
  • Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. 
  • Investigation of the influence on conformational transition of DNA induced by cationic lipid vesicles. 
  • Isolation and characterization of the major apolipoprotein from chicken high density lipoproteins. 
  • Kar3 interaction with Cik1 alters motor structure and function. 
  • Left-handed DNA helices, supercoiling, and the B-Z junction. 
  • Linked folding and anion binding of the Bacillus subtilis ribonuclease P protein. 
  • Liposome-mediated conformation transition of DNA detected by molecular probe: methyl green. 
  • Mapping Tertiary Interactions in Protein Folding Reactions: A Novel Mass Spectrometry- and Chemical Synthesis-Based Approach 
  • Mapping the binding sites of human erythrocyte ankyrin for the anion exchanger and spectrin. 
  • Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. 
  • Metal-Organic Framework Encapsulation Preserves the Bioactivity of Protein Therapeutics. 
  • Methionine oxidation of monomeric lambda repressor: the denatured state ensemble under nondenaturing conditions. 
  • Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation. 
  • Multiple structural elements define the specificity of recombinant human inhibitor-1 as a protein phosphatase-1 inhibitor. 
  • Mutational Dissection of Telomeric DNA Binding Requirements of G4 Resolvase 1 Shows that G4-Structure and Certain 3'-Tail Sequences Are Sufficient for Tight and Complete Binding. 
  • Mutational analysis of active site residues in the Staphylococcus aureus transpeptidase SrtA. 
  • Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX. 
  • Mutations that destabilize the gp41 core are determinants for stabilizing the simian immunodeficiency virus-CPmac envelope glycoprotein complex. 
  • Novel disulfide engineering in human carbonic anhydrase II using the PAIRWISE side-chain geometry database. 
  • Optical signatures of molecular dissymmetry: combining theory with experiments to address stereochemical puzzles. 
  • Optimized production and analysis of the staphylococcal multidrug efflux protein QacA. 
  • Osmolyte-induced folding of an intrinsically disordered protein: folding mechanism in the absence of ligand. 
  • Oxygenated norethindrone derivatives from incubation with beagle liver. Structure, synthesis, and biological activity. 
  • Pathogenic Mutations Induce Partial Structural Changes in the Native β-Sheet Structure of Transthyretin and Accelerate Aggregation. 
  • Peptide binding proclivities of calcium loaded calbindin-D28k. 
  • Phosphorylation of RNA polymerase II CTD fragments results in tight binding to the WW domain from the yeast prolyl isomerase Ess1. 
  • Phosphorylation-dependent metal binding by alpha-synuclein peptide fragments. 
  • Physical properties of human alpha 2-macroglobulin following reaction with methylamine and trypsin. 
  • Physicochemical properties of rat testis Ca2+-dependent regulator protein of cyclic nucleotide phosphodiesterase. Relationship of Ca2+-binding, conformational changes, and phosphodiesterase activity. 
  • Portable UV-visible spectrometer for measuring absorbance and dichroism of Langmuir monolayers at air-water interfaces. 
  • Prediction of secondary structure for Eco RI endonuclease. 
  • Preorganized secondary structure as an important determinant of fast protein folding. 
  • Probing the oligomeric structure of an enzyme by electrospray ionization time-of-flight mass spectrometry. 
  • Propensity for a leucine zipper-like domain of human immunodeficiency virus type 1 gp41 to form oligomers correlates with a role in virus-induced fusion rather than assembly of the glycoprotein complex. 
  • Protein design and folding: template trapping of self-assembled helical bundles. 
  • Purification and characterization of a "half-molecule" alpha 2-macroglobulin from the southern grass frog: absence of binding to the mammalian alpha 2-macroglobulin receptor. 
  • Purification and characterization of frog alpha-macroglobulin: receptor recognition of an amphibian glycoprotein. 
  • R73A and H144Q mutants of the yeast mitochondrial cyclophilin Cpr3 exhibit a low prolyl isomerase activity in both peptide and protein-folding assays. 
  • Rational design of a calcium-binding protein. 
  • Recognition of lysine-rich peptide ligands by murine cortactin SH3 domain: CD, ITC, and NMR studies. 
  • Remote oxidation of steroids by photolysis of attached benzophenone groups. 
  • Removal of the pro-domain does not affect the conformation of the procaspase-3 dimer. 
  • Robust IR-based detection of stable and fractionally populated G-C+ and A-T Hoogsteen base pairs in duplex DNA. 
  • Rod mutations associated with MYH9-related disorders disrupt nonmuscle myosin-IIA assembly. 
  • Selection of immunoglobulin elbow region mutations impacts interdomain conformational flexibility in HIV-1 broadly neutralizing antibodies. 
  • Sequence- or position-specific mutations in the carboxyl-terminal FL motif of the kidney sodium bicarbonate cotransporter (NBC1) disrupt its basolateral targeting and alpha-helical structure. 
  • Sequence-specific B-DNA flexibility modulates Z-DNA formation. 
  • Single-handed helical wrapping of single-walled carbon nanotubes by chiral, ionic, semiconducting polymers. 
  • Six-helix bundle assembly and characterization of heptad repeat regions from the F protein of Newcastle disease virus. 
  • Solvent dependence of intramolecular electron transfer in a helical oligoproline assembly. 
  • Specific 33-residue repeat(s) of erythrocyte ankyrin associate with the anion exchanger. 
  • Stability studies on a lipase from Bacillus subtilis in guanidinium chloride. 
  • Stable docking of neutralizing human immunodeficiency virus type 1 gp41 membrane-proximal external region monoclonal antibodies 2F5 and 4E10 is dependent on the membrane immersion depth of their epitope regions. 
  • Stable dry powder formulation for nasal delivery of anthrax vaccine. 
  • Structural adaptations that modulate monosaccharide, disaccharide, and trisaccharide specificities in periplasmic maltose-binding proteins. 
  • Structural analysis of the purine repressor, an Escherichia coli DNA-binding protein. 
  • Structural analysis of the unmutated ancestor of the HIV-1 envelope V2 region antibody CH58 isolated from an RV144 vaccine efficacy trial vaccinee. 
  • Structural basis for the remarkable stability of Bacillus subtilis lipase (Lip A) at low pH. 
  • Structural mechanism of the simultaneous binding of two drugs to a multidrug-binding protein. 
  • Structural organization of the human insulin receptor ectodomain. 
  • Structural studies of the heme domain of sulfite oxidase: CNBr fragments. 
  • Structure and stability of monomeric lambda repressor: NMR evidence for two-state folding. 
  • Structure of macromolecular aggregates. II. Construction of model membranes from phospholipids and polypeptides. 
  • Systematic assignment of the configuration of flexible natural products by spectroscopic and computational methods: the bistramide C analysis. 
  • The basic helix-loop-helix domain of the aryl hydrocarbon receptor nuclear transporter (ARNT) can oligomerize and bind E-box DNA specifically. 
  • The domains of rat liver sulfite oxidase. Proteolytic separation and characterization. 
  • The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex. 
  • The effects of lipid composition on the binding of lasalocid A to small unilamellar vesicles. 
  • The human ACC2 CT-domain C-terminus is required for full functionality and has a novel twist. 
  • The hydrophobic core of Escherichia coli thioredoxin shows a high tolerance to nonconservative single amino acid substitutions. 
  • The membrane-binding domain of ankyrin contains four independently folded subdomains, each comprised of six ankyrin repeats. 
  • The site of the redox-linked proton pump in eukaryotic cytochrome c oxidases. 
  • Thermodynamic characterization of the osmolyte- and ligand-folded states of Bacillus subtilis ribonuclease P protein. 
  • Thermodynamics of RNA melting, one base pair at a time. 
  • Using alpha-helical coiled-coils to design nanostructured metalloporphyrin arrays. 
  • VSV transmembrane domain (TMD) peptide promotes PEG-mediated fusion of liposomes in a conformationally sensitive fashion. 
  • Yin Yang 1 contains G-quadruplex structures in its promoter and 5'-UTR and its expression is modulated by G4 resolvase 1. 
  • pH-dependent conformational changes of ferricytochrome c induced by electrode surface microstructure. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Oas, Terrence Gilbert, Professor of Biochemistry, Biochemistry