Crystallization

• 

  Subject Areas on Research

  • A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. 
  • Alkali action on the urinary crystallization of calcium salts: contrasting responses to sodium citrate and potassium citrate. 
  • Allosteric regulation of type I hexokinase: A site-directed mutational study indicating location of the functional glucose 6-phosphate binding site in the N-terminal half of the enzyme. 
  • Alpha-carbon coordinates for bovine Cu,Zn superoxide dismutase. 
  • Alternate states of proteins revealed by detailed energy landscape mapping. 
  • Aluminum nanocrystals. 
  • Applications of nanotechnology to biotechnology commentary. 
  • Assembly of colloidal molecules, polymers, and crystals in acoustic and magnetic fields. 
  • B-DNA to zip-DNA: simulating a DNA transition to a novel structure with enhanced charge-transport characteristics. 
  • Calcium stone disease: an overview. 
  • Cathepsin B1. A lysosomal enzyme that degrades native collagen. 
  • Characterizing protein crystal contacts and their role in crystallization: rubredoxin as a case study. 
  • Chemical synthesis of novel plasmonic nanoparticles. 
  • Classification of crystallization outcomes using deep convolutional neural networks. 
  • Cocrystal structure of protein farnesyltransferase complexed with a farnesyl diphosphate substrate. 
  • Collagen-rich crystalloids in a scarred vascularized cornea. 
  • Computational Methods for RNA Structure Validation and Improvement. 
  • Computational crystallization. 
  • Computational studies of small carbon and iron-carbon systems relevant to carbon nanotube growth. 
  • Contributions of hydration and steric (entropic) pressures to the interactions between phosphatidylcholine bilayers: experiments with the subgel phase. 
  • Coordination-induced formation of submicrometer-scale, monodisperse, spherical colloids of organic-inorganic hybrid materials at room temperature. 
  • Crystal structure and sequence-dependent conformation of the A.G mispaired oligonucleotide d(CGCAAGCTGGCG). 
  • Crystal structure of alkaline phosphatase from the Antarctic bacterium TAB5. 
  • Crystal structure of farnesyl protein transferase complexed with a CaaX peptide and farnesyl diphosphate analogue. 
  • Crystal structure of ferrioxamine B: a comparative analysis and implications for molecular recognition. 
  • Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase. 
  • Crystal structure of the promutagen O4-methylthymidine: importance of the anti conformation of the O(4) methoxy group and possible mispairing of O4-methylthymidine with guanine. 
  • Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani. 
  • Crystal structures of the anticancer clinical candidates R115777 (Tipifarnib) and BMS-214662 complexed with protein farnesyltransferase suggest a mechanism of FTI selectivity. 
  • Crystalline arrays of the Escherichia coli sn-glycerol-3-phosphate acyltransferase, an integral membrane protein. 
  • Crystalline extracellular nuclease of Staphylococcus aureus. 
  • Crystallization and initial X-ray crystallographic characterization of recombinant bovine inositol polyphosphate 1-phosphatase produced in Spodoptera frugiperda cells. 
  • Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex. 
  • Crystallization and preliminary X-ray crystallographic investigations on several thermostable forms of a Bacillus subtilis lipase. 
  • Crystallization and preliminary X-ray crystallographic studies of glutaredoxin 2 from Saccharomyces cerevisiae in different oxidation states. 
  • Crystallization and preliminary X-ray diffraction studies on the DNA-binding domain of the multidrug transporter activation protein (MtaN) from Bacillus subtilis. 
  • Crystallization and preliminary X-ray investigation of calmodulin. 
  • Crystallization and preliminary diffraction analysis of a hyperthermostable DNA polymerase from a Thermococcus archaeon. 
  • Crystallization and preliminary x-ray studies of NADPH-cytochrome P450 reductase. 
  • Crystallization of a Complex Between MYC and Jas Motif. 
  • Crystallization of a complex of cro repressor with a 17 base-pair operator. 
  • Crystallization of a fragment of human fibronectin: introduction of methionine by site-directed mutagenesis to allow phasing via selenomethionine. 
  • Crystallization of asymmetric patchy models for globular proteins in solution 
  • Crystallization of succinylated concanavalin A bound to a synthetic bivalent ligand and preliminary structural analysis. 
  • Crystallization of the bifunctional biotin operon repressor. 
  • Crystallization of the purine salvage enzyme adenine phosphoribosyltransferase. 
  • Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae. 
  • Crystalloids in salivary gland pleomorphic adenomas. 
  • Cytochrome oxidase: structural insights from electron microscopy and from secondary structure prediction. 
  • Decoupling local disorder and optical effects in infrared spectra: differentiating between calcites with different origins. 
  • Deep UV nano-microstructuring of substrates for surface plasmon resonance imaging. 
  • Designing an extracellular matrix protein with enhanced mechanical stability. 
  • Destructive tophaceous calcium hydroxyapatite tumor of the infratemporal fossa. Case report and review of the literature. 
  • Diagnosis of crystal induced arthritis by dark field microscopy. 
  • Discrete fracture patterns of virus shells reveal mechanical building blocks. 
  • Drug design from the cryptic inhibitor envelope. 
  • Evaluation of biaxial mechanical properties of soft tubes and arteries using sonometry. 
  • Expression, purification and primary crystallographic study of human androgen receptor in complex with DNA and coactivator motifs. 
  • Expression, purification, crystallization and preliminary X-ray analysis of human argininosuccinic acid lyase. 
  • Fabrication of gold nanowires by electric-field-induced scanning probe lithography and in situ chemical development. 
  • Fabrication of high quality carbon nanotubes with a simple ethanol-assisted arc discharge process. 
  • Fabrication of small diameter few-walled carbon nanotubes with enhanced field emission property. 
  • Fabrication of surface plasmon resonators by nanoskiving single-crystalline gold microplates. 
  • Functional significance of the central helix in calmodulin. 
  • Functionalized few-walled carbon nanotubes for mechanical reinforcement of polymeric composites. 
  • Functionalized graphene sheets for polymer nanocomposites. 
  • Gallbladder Na+/H+ exchange activity is up-regulated prior to cholesterol crystal formation. 
  • Generation, characterization and crystallization of a highly active and stable cytochrome bc1 complex mutant from Rhodobacter sphaeroides. 
  • Gold nanocages: bioconjugation and their potential use as optical imaging contrast agents. 
  • Hard-sphere crystallization gets rarer with increasing dimension. 
  • High resolution AFM topographs of the Escherichia coli water channel aquaporin Z. 
  • Highly parallel acoustic assembly of microparticles into well-ordered colloidal crystallites. 
  • Influenza immunization elicits antibodies specific for an egg-adapted vaccine strain. 
  • Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. 
  • Initiating a structural study of 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. 
  • Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields. 
  • Learning about Biomolecular Solvation from Water in Protein Crystals. 
  • Lipopolysaccharide bilayer structure: effect of chemotype, core mutations, divalent cations, and temperature. 
  • Localized delivery of DNA to the cells by viral collagen-loaded silica colloidal crystals. 
  • Mechanically interlocked calix[4]arene dimers display reversible bond breakage under force. 
  • Membranous cytochrome c oxidase. A freeze-fracture electron microscopic analysis. 
  • Metabolic diagnosis and medical prevention of calcium nephrolithiasis and its systemic manifestations: a consensus statement. 
  • MolProbity: all-atom structure validation for macromolecular crystallography. 
  • Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB. 
  • Multivalency effects in protein--carbohydrate interaction: the binding of the Shiga-like toxin 1 binding subunit to multivalent C-linked glycopeptides. 
  • Nature of Amorphous Hydrophilic Block Affects Self-Assembly of an Artificial Viral Coat Polypeptide. 
  • Negatively stained vinblastine aggregates. 
  • Neoplastic plasma cells with azurophilic crystalline inclusions mimicking promyelocytes. 
  • Novel strategy for diameter-selective separation and functionalization of single-wall carbon nanotubes. 
  • Nucleic acid binding drugs--XIV. The crystal structure of 1-methyl amsacrine hydrochloride; relationships to DNA-binding ability and anti-tumour activity. 
  • One-dimensional crystals of (Na+ + K+)-ATPase dimers. 
  • One-pot synthesis of CdTe nanocrystals and shape control of luminescent CdTe-cystine nanocomposites. 
  • Optical near-field mapping of plasmonic nanoprisms. 
  • Oxalate deposition on asbestos bodies. 
  • Paracrystalline inclusions associated with the disk membranes of frog retinal rod outer segments. 
  • Phonon localization drives polar nanoregions in a relaxor ferroelectric. 
  • Physical properties of cholesteryl esters. 
  • Preliminary x-ray diffraction studies of EcoRI restriction endonuclease-DNA complex. 
  • Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations. 
  • Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9. 
  • Promoter recognition by a complex of Spx and the C-terminal domain of the RNA polymerase alpha subunit. 
  • Pulmonary oxalate deposition associated with Aspergillus niger infection. An oxidant hypothesis of toxicity. 
  • Purification of semiconducting carbon nanotubes. 
  • Purification, crystallization and preliminary X-ray diffraction analysis of the human mismatch repair protein MutSβ. 
  • Purification, crystallization and preliminary X-ray diffraction studies of a complex between G protein-coupled receptor kinase 2 and Gbeta1gamma2. 
  • Quantification of the binding affinity of a specific hydroxyapatite binding peptide. 
  • Rapid and quantitative imaging of excitation polarized fluorescence reveals ordered septin dynamics in live yeast. 
  • Reassessing serum urate targets in the management of refractory gout: can you go too low? 
  • Relaxation spectra of adenosine triphosphate-creatine phosphotransferase. 
  • Resistance differences between blood and crystalloid cardioplegic solutions with myocardial cooling. 
  • Reverse actin sliding triggers strong myosin binding that moves tropomyosin. 
  • Right bipyramids of silver: a new shape derived from single twinned seeds. 
  • Room temperature purification of few-walled carbon nanotubes with high yield. 
  • Scaling behavior of nanopatterned polymer brushes. 
  • Self-assembly of ordered nanowires in biological suspensions of single-wall carbon nanotubes. 
  • Shape-controlled synthesis of metal nanostructures: the case of silver. 
  • Size-controlled electrochemical synthesis and properties of SnO2 nanotubes. 
  • Size-dependence of surface plasmon resonance and oxidation for Pd nanocubes synthesized via a seed etching process. 
  • Soft matter perspective on protein crystal assembly. 
  • Stepwise self-assembly of DNA tile lattices using dsDNA bridges. 
  • Structural analyses of Candida albicans sterol 14α-demethylase complexed with azole drugs address the molecular basis of azole-mediated inhibition of fungal sterol biosynthesis. 
  • Structural and mechanical study of a self-assembling protein nanotube. 
  • Structural basis of multifunctional bovine mitochondrial cytochrome bc1 complex. 
  • Structural interconversions modulate activity of Escherichia coli ribonucleotide reductase. 
  • Structural mechanisms of QacR induction and multidrug recognition. 
  • Structure and function of the visual arrestin oligomer. 
  • Structure and mechanical properties of giant lipid (DMPC) vesicle bilayers from 20 degrees C below to 10 degrees C above the liquid crystal-crystalline phase transition at 24 degrees C. 
  • Structure and thermomechanical behavior of NiTiPt shape memory alloy wires. 
  • Structure of DNA polymerase I Klenow fragment bound to duplex DNA. 
  • Structure of a fibronectin type III domain from tenascin phased by MAD analysis of the selenomethionyl protein. 
  • Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane. 
  • Structure of the crystalline bilayer in the subgel phase of dipalmitoylphosphatidylglycerol. 
  • Structure of the cytochrome c oxidase dimer. Electron microscopy of two-dimensional crystals. 
  • Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis. 
  • Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution. 
  • Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography. 
  • Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation. 
  • Structures of regulatory machinery reveal novel molecular mechanisms controlling B. subtilis nitrogen homeostasis. 
  • Structures of the siroheme- and Fe4S4-containing active center of sulfite reductase in different states of oxidation: heme activation via reduction-gated exogenous ligand exchange. 
  • Struvite calculi. 
  • Supramolecular nanomimetics: replication of micelles, viruses, and other naturally occurring nanoscale objects. 
  • Surface-stress-induced phase transformation in metal nanowires. 
  • Synovial and systemic pharmacokinetics (PK) of triamcinolone acetonide (TA) following intra-articular (IA) injection of an extended-release microsphere-based formulation (FX006) or standard crystalline suspension in patients with knee osteoarthritis (OA). 
  • Synthesis and electrical characterization of silver nanobeams. 
  • Synthesis and optical properties of silver nanobars and nanorice. 
  • Synthesis of Double-walled Carbon Nanotubes Using Iron Disilicide as Catalyst 
  • Synthesis of high-density, large-diameter, and aligned single-walled carbon nanotubes by multiple-cycle growth methods. 
  • Synthesis of oxidation-resistant cupronickel nanowires for transparent conducting nanowire networks. 
  • Synthesis of ultra-short-acting neuromuscular blocker GW 0430: a remarkably stereo- and regioselective synthesis of mixed tetrahydroisoquinolinium chlorofumarates. 
  • Synthesis, characterization, and cyclic stress-influenced degradation of a poly(ethylene glycol)-based poly(beta-amino ester). 
  • Terahertz electromagnetic crystal waveguide fabricated by polymer jetting rapid prototyping. 
  • The 2.1 A structure of Aerococcus viridans L-lactate oxidase (LOX). 
  • The 3.7 A projection map of the glycerol facilitator GlpF: a variant of the aquaporin tetramer. 
  • The Crystal Structure and Cytotoxicity of Goniodiol Monoacetate from Goniothalamus Amyon 
  • The aquaporin sidedness revisited. 
  • The crystal structure of bovine Cu2+,Zn2+ superoxide dismutase at 5.5-A resolution. 
  • The crystal structure of the UvsW helicase from bacteriophage T4. 
  • The crystal structure of the collagen-like polypeptide (glycyl-4(R)-hydroxyprolyl-4(R)-hydroxyprolyl)9 at 1.55 A resolution shows up-puckering of the proline ring in the Xaa position. 
  • The extracellular nuclease of Staphylococcus aureus: structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution. 
  • The high-throughput protein-to-structure pipeline at SECSG. 
  • The role of apoptosis in the initiation of vascular calcification. 
  • The role of thickness transitions in convective assembly. 
  • Three-dimensional membrane crystals in amphibian cone outer segments. 1. Light-dependent crystal formation in frog retinas. 
  • Tryptic cleavage of rat liver sulfite oxidase. Isolation and characterization of molybdenum and heme domains. 
  • Two crystal forms of bovine superoxide dismutase. 
  • Two-dimensional crystallization and projection structure of KcsA potassium channel. 
  • Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines. 
  • Two-dimensional rhodopsin crystals from disk membranes of frog retinal rod outer segments. 
  • Ultrastructure of the human aortic fibrolipid lesion. Formation of the atherosclerotic lipid-rich core. 
  • Variation of molecular alignment as a means of resolving orientational ambiguities in protein structures from dipolar couplings. 
  • Visualizing multistep elevator-like transitions of a nucleoside transporter. 
  • West African crystalline maculopathy. 
• 

  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Preminger, Glenn Michael, James F. Glenn, M.D. Distinguished Professor of Urology, Surgery, Urology