Cysteine

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  Subject Areas on Research

  • (1)H MRS detection of glycine residue of reduced glutathione in vivo. 
  • A Multiplex Enzymatic Machinery for Cellular Protein S-nitrosylation. 
  • A comparison of methionine, histidine and cysteine in copper(I)-binding peptides reveals differences relevant to copper uptake by organisms in diverse environments. 
  • A comprehensive search for calcium binding sites critical for TMEM16A calcium-activated chloride channel activity. 
  • A genetic analysis of nitrosative stress. 
  • A metabolic signature of colon cancer initiating cells. 
  • A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity. 
  • A nitric oxide/cysteine interaction mediates the activation of soluble guanylate cyclase. 
  • A protein microarray-based analysis of S-nitrosylation. 
  • A sodium channel mutation identified in Aedes aegypti selectively reduces cockroach sodium channel sensitivity to type I, but not type II pyrethroids. 
  • A sulfurtransferase is required in the transfer of cysteine sulfur in the in vitro synthesis of molybdopterin from precursor Z in Escherichia coli. 
  • Activity and distribution of the cysteine prodrug activating enzyme, 5-oxo-L-prolinase, in human normal and tumor tissues. 
  • Altering the Immunogenicity of Hemagglutinin Immunogens by Hyperglycosylation and Disulfide Stabilization. 
  • An HPLC assay for detection of elevated urinary S-sulphocysteine, a metabolic marker of sulphite oxidase deficiency. 
  • Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor. 
  • Analysis of renal cell transformation following exposure to trichloroethene in vivo and its metabolite S-(dichlorovinyl)-L-cysteine in vitro. 
  • Analysis of the structural properties of cAMP-responsive element-binding protein (CREB) and phosphorylated CREB. 
  • Antagonists of the system L neutral amino acid transporter (LAT) promote endothelial adhesivity of human red blood cells. 
  • Ascaris haemoglobin is a nitric oxide-activated 'deoxygenase'. 
  • Assessment and application of the biotin switch technique for examining protein S-nitrosylation under conditions of pharmacologically induced oxidative stress. 
  • Atomic distance estimates from disulfides and high-affinity metal-binding sites in a K+ channel pore. 
  • Biomarkers in the diagnosis of anaphylaxis: making nature disclose her mysteries. 
  • Biotransformation of L-cysteine S-conjugates and N-acetyl-L-cysteine S-conjugates of the sevoflurane degradation product fluoromethyl-2,2-difluoro-1-(trifluoromethyl)vinyl ether (compound A) in human kidney in vitro: interindividual variability in N-acetylation, N-deacetylation, and beta-lyase-catalyzed metabolism. 
  • Blood biomarkers of methylation in Down syndrome and metabolic simulations using a mathematical model. 
  • Blood flow regulation by S-nitrosohemoglobin in the physiological oxygen gradient. 
  • Both selenium deficiency and modest selenium supplementation lead to myocardial fibrosis in mice via effects on redox-methylation balance. 
  • Can prenylcysteines be exploited as ligands for mammalian multidrug-resistance transporters? 
  • Cardioprotective role of S-nitrosylated hemoglobin from rbc. 
  • Cathepsin B1. A lysosomal enzyme that degrades native collagen. 
  • Characterization of prenylcysteines that interact with P-glycoprotein and inhibit drug transport in tumor cells. 
  • Chemical modification of alpha 2-adrenoceptors. Possible role for tyrosine in the ligand binding site. 
  • Clamp subunit dissociation dictates bacteriophage T4 DNA polymerase holoenzyme disassembly. 
  • Complexes of serum albumin and cis-dichlorodiammineplatinum (II). The role of cysteine 34 as a nucleophilic entering group and evidence for reaction between bound platinum and a second macromolecule. 
  • Compound A uptake and metabolism to mercapturic acids and 3,3,3-trifluoro-2-fluoromethoxypropanoic acid during low-flow sevoflurane anesthesia: biomarkers for exposure, risk assessment, and interspecies comparison. 
  • Confirmation of the involvement of protein domain movement during the catalytic cycle of the cytochrome bc1 complex by the formation of an intersubunit disulfide bond between cytochrome b and the iron-sulfur protein. 
  • Conformational changes at the carboxyl terminus of Galpha occur during G protein activation. 
  • Constitutive activation of the N-methyl-D-aspartate receptor via cleft-spanning disulfide bonds. 
  • Construction of a family of Cys2His2 zinc binding sites in the hydrophobic core of thioredoxin by structure-based design. 
  • Construction of a novel redox protein by rational design: conversion of a disulfide bridge into a mononuclear iron-sulfur center. 
  • Control of lens epithelial cell survival. 
  • Control of protein-ligand recognition using a stimuli-responsive polymer. 
  • Cortisol responses to emotional stress in men: association with a functional polymorphism in the 5HTR2C gene. 
  • Coupling of proton binding in extracellular domain to channel gating in acid-sensing ion channel. 
  • Covalent targeting of remote cysteine residues to develop CDK12 and CDK13 inhibitors. 
  • Crystal structure of human type III collagen Gly991-Gly1032 cystine knot-containing peptide shows both 7/2 and 10/3 triple helical symmetries. 
  • Cysteine 70 of ankyrin-G is S-palmitoylated and is required for function of ankyrin-G in membrane domain assembly. 
  • Cysteine and glutathione levels in developing rat kidney and liver. 
  • Cysteine-induced modifications of zero-valent silver nanomaterials: Implications for particle surface chemistry, aggregation, dissolution, and silver speciation 
  • Cysteine-rich regions of pig gastric mucin contain von willebrand factor and cystine knot domains at the carboxyl terminal(1). 
  • Cysteine-tailed class I-binding peptides bind to CpG adjuvant and enhance primary CTL responses. 
  • Cysteines in the transmembrane region of major histocompatibility complex antigens are fatty acylated via thioester bonds. 
  • Cystine addiction of triple-negative breast cancer associated with EMT augmented death signaling. 
  • Cytotoxicity of S-conjugates of the sevoflurane degradation product fluoromethyl-2,2-difluoro-1-(trifluoromethyl) vinyl ether (Compound A) in a human proximal tubular cell line. 
  • Degradation of collagen by a human granulocyte collagenolytic system. 
  • Deletion of cysteine 369 in lysyl hydroxylase 1 eliminates enzyme activity and causes Ehlers-Danlos syndrome type VI. 
  • Dendritic macromers as in situ polymerizing biomaterials for securing cataract incisions. 
  • Detection of protein S-nitrosylation with the biotin-switch technique. 
  • Dietary isothiocyanate-induced apoptosis via thiol modification of DNA topoisomerase IIα. 
  • Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1. 
  • Discovery of Selective Estrogen Receptor Covalent Antagonists for the Treatment of ERαWT and ERαMUT Breast Cancer. 
  • Disulfide bond requirements for active Wnt ligands. 
  • Effect of cysteine residues on the activity of arginyl-tRNA synthetase from Escherichia coli. 
  • Effect of cystine loading on substrate oxidation by rat renal tubules. 
  • Effect of hypophysectomy on somatostatin-14 and somatostatin-28 biosynthesis in the rat hypothalamus. 
  • Effects of S-nitrosation on oxygen binding by normal and sickle cell hemoglobin. 
  • Effects of a block in cysteine catabolism on energy balance and fat metabolism in mice. 
  • Essential roles of S-nitrosothiols in vascular homeostasis and endotoxic shock. 
  • Evidence for a catalytic role of zinc in protein farnesyltransferase. Spectroscopy of Co2+-farnesyltransferase indicates metal coordination of the substrate thiolate. 
  • Experimentally Dissecting the Origins of Peroxiredoxin Catalysis. 
  • Eyedrops containing SA9000 prodrugs result in sustained reductions in intraocular pressure in rabbits. 
  • FTY720 stimulates multidrug transporter- and cysteinyl leukotriene-dependent T cell chemotaxis to lymph nodes. 
  • Fluorescent probes as indicators of conformation changes in transducin on activation. 
  • Functional properties of hemoglobin Pôrto Alegre (alpha2A beta2 9Ser leads to Cys) and the reactivity of its extra cysteinyl residue. 
  • Functional reconstitution and characterization of AqpZ, the E. coli water channel protein. 
  • Genetic and pharmacologic analyses of the role of Icmt in Ras membrane association and function. 
  • Glutamine Metabolism Regulates the Pluripotency Transcription Factor OCT4. 
  • H-Ras peptide and protein substrates bind protein farnesyltransferase as an ionized thiolate. 
  • HNF4α regulates sulfur amino acid metabolism and confers sensitivity to methionine restriction in liver cancer. 
  • Heme-assisted S-nitrosation desensitizes ferric soluble guanylate cyclase to nitric oxide. 
  • Hydrogen peroxide sensor HPCA1 is an LRR receptor kinase in Arabidopsis. 
  • Identification of a cysteine residue essential for activity of protein farnesyltransferase. Cys299 is exposed only upon removal of zinc from the enzyme. 
  • Impacts of silver nanoparticle coating on the nitrification potential of Nitrosomonas europaea. 
  • In vivo biosynthesis of arginine vasopressin and oxytocin in hypothalami from intact and hypophysectomized rats. 
  • Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes. 
  • Influence of amino acids cysteine and serine on aggregation kinetics of zinc and mercury sulfide colloids 
  • Inhibition of NF-kappa B by S-nitrosylation. 
  • Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses. 
  • Interaction of prenylcysteine methyl esters with the multidrug resistance transporter. 
  • Interaction sites of the C-terminal region of the cGMP phosphodiesterase inhibitory subunit with the GDP-bound transducin alpha-subunit. 
  • Internal electron transfer between hemes and Cu(II) bound at cysteine beta93 promotes methemoglobin reduction by carbon monoxide. 
  • Investigating the SecY plug movement at the SecYEG translocation channel. 
  • Investigations into the loss of glutathione from lenses in organ culture. 
  • Isolation and characterization of a prenylcysteine lyase from bovine brain. 
  • Juvenile bilateral lens dislocation and glaucoma associated with a novel mutation in the fibrillin 1 gene. 
  • Keap1 modification and nuclear accumulation in response to S-nitrosocysteine. 
  • LAT palmitoylation: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation. 
  • Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins. 
  • Lysosomal prenylcysteine lyase is a FAD-dependent thioether oxidase. 
  • Manganese-based superoxide dismutase mimics modify both acute and long-term outcome severity in a Drosophila melanogaster model of classic galactosemia. 
  • Mapping of a ligand-binding site for the human thromboxane A2 receptor protein. 
  • Mapping staphylococcal nuclease conformation using an EDTA-Fe derivative attached to genetically engineered cysteine residues. 
  • Mechanism of action of a respiratory inhibitor from the gill tissue of the sporulating common mushroom, Agaricus bisporus. 
  • Missense mutation (G480C) in the CFTR gene associated with protein mislocalization but normal chloride channel activity. 
  • Modification of cysteine 111 in human Cu,Zn-superoxide dismutase. 
  • Modulation of cytotoxicity of menadione sodium bisulfite versus leukemia L1210 by the acid-soluble thiol pool. 
  • Molecular mechanical properties of short-sequence peptide enzyme mimics. 
  • NADPH:quinone oxidoreductase 1 regulates host susceptibility to ozone via isoprostane generation. 
  • Neonatal nonepileptic myoclonus is a prominent clinical feature of KCNQ2 gain-of-function variants R201C and R201H. 
  • Nephrolithiasis in identical twins: the impact of nature vs nurture. 
  • Nephrotoxicity of sevoflurane compound A [fluoromethyl-2,2-difluoro-1-(trifluoromethyl)vinyl ether] in rats: evidence for glutathione and cysteine conjugate formation and the role of renal cysteine conjugate beta-lyase. 
  • Nitric oxide-generating hydrogels inhibit neointima formation. 
  • Nucleotide metabolism is linked to cysteine availability. 
  • Orthogonal site-specific protein modification by engineering reversible thiol protection mechanisms. 
  • Oxidation-induced intramolecular disulfide bond inactivates mitogen-activated protein kinase kinase 6 by inhibiting ATP binding. 
  • Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina. 
  • Palmitoylation of G protein-coupled receptor kinase, GRK6. Lipid modification diversity in the GRK family. 
  • Palmitoylation of the human beta 2-adrenergic receptor. Mutation of Cys341 in the carboxyl tail leads to an uncoupled nonpalmitoylated form of the receptor. 
  • Pathways of folding of reduced bovine pancreatic ribonuclease. 
  • Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity. 
  • Plant Immunity Requires Conformational Changes of NPR1 via S-Nitrosylation and Thioredoxins 
  • Precocious osteoarthritis in a family with recurrent COL2A1 mutation. 
  • Prenylcysteine lyase deficiency in mice results in the accumulation of farnesylcysteine and geranylgeranylcysteine in brain and liver. 
  • Probing the architecture of the B. subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage. 
  • Probing the folded state of fibronectin type III domains in stretched fibrils by measuring buried cysteine accessibility. 
  • Protease activity of normal and PHA stimulated human lymphocytes. 
  • Protein S-nitrosylation: purview and parameters. 
  • Protein farnesyltransferase: kinetics of farnesyl pyrophosphate binding and product release. 
  • Protein polymer hydrogels by in situ, rapid and reversible self-gelation. 
  • Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture. 
  • Pseudomonas exotoxin A mutants. Replacement of surface exposed residues in domain II with cysteine residues that can be modified with polyethylene glycol in a site-specific manner. 
  • Pulmonary alveolar epithelial uptake of S-nitrosothiols is regulated by L-type amino acid transporter. 
  • Pulsed EPR studies of nonexchangeable protons near the Mo(V) center of sulfite oxidase: direct detection of the alpha-proton of the coordinated cysteinyl residue and structural implications for the active site. 
  • Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response. 
  • Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer. 
  • Regulation of HIF-1alpha stability through S-nitrosylation. 
  • Regulation of beta-adrenergic receptor signaling by S-nitrosylation of G-protein-coupled receptor kinase 2. 
  • Regulation of mitochondrial processes by protein S-nitrosylation. 
  • Responses of normal and sickle cell hemoglobin to S-nitroscysteine: implications for therapeutic applications of NO in treatment of sickle cell disease. 
  • RoBo-1, a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and growth plate cartilage. 
  • Role of conserved gp41 cysteine residues in the processing of human immunodeficiency virus envelope precursor and viral infectivity. 
  • Role of cytochrome P4503A in cysteine S-conjugates sulfoxidation and the nephrotoxicity of the sevoflurane degradation product fluoromethyl-2,2-difluoro-1-(trifluoromethyl)vinyl ether (compound A) in rats. 
  • Role of extracellular disulfide-bonded cysteines in the ligand binding function of the beta 2-adrenergic receptor. 
  • Role of granulocyte collagenase in collagen degradation. 
  • S-Nitrosylation of Sarcomeric Proteins Depresses Myofilament Ca2+)Sensitivity in Intact Cardiomyocytes. 
  • S-nitrosohaemoglobin: a dynamic activity of blood involved in vascular control. 
  • S-nitrosothiols and the S-nitrosoproteome of the cardiovascular system. 
  • S-nitrosylation TRiPs a calcium switch. 
  • S-nitrosylation in health and disease. 
  • S-nitrosylation of beta-arrestin regulates beta-adrenergic receptor trafficking. 
  • Selenocysteine substitutions in thiyl radical enzymes. 
  • Seven novel mutations in the adenosine deaminase (ADA) gene in patients with severe and delayed onset combined immunodeficiency: G74C, V129M, G140E, R149W, Q199P, 462delG, and E337del. Mutations in brief no. 142. Online. 
  • Sex and race differences of cerebrospinal fluid metabolites in healthy individuals. 
  • Single residues in the outer pore of TRPV1 and TRPV3 have temperature-dependent conformations. 
  • Site-directed mutagenesis of glycine-14 and two "critical" cysteinyl residues in Drosophila alcohol dehydrogenase. 
  • Site-directed mutagenesis of recombinant sulfite oxidase: identification of cysteine 207 as a ligand of molybdenum. 
  • Site-specific PEGylation of hemoglobin at Cys-93(beta): correlation between the colligative properties of the PEGylated protein and the length of the conjugated PEG chain. 
  • Site-specific analysis of protein S-acylation by resin-assisted capture. 
  • Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. 
  • Stabilization of HIV-1 envelope in the CD4-bound conformation through specific cross-linking of a CD4 mimetic. 
  • Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation. 
  • Staphylococcus aureus sortase A contributes to the Trojan horse mechanism of immune defense evasion with its intrinsic resistance to Cys184 oxidation. 
  • Staphylococcus aureus sortase transpeptidase SrtA: insight into the kinetic mechanism and evidence for a reverse protonation catalytic mechanism. 
  • Strategies for dissecting genetic-environmental interactions in neurodegenerative disorders. 
  • Structural Insights into Electrophile Irritant Sensing by the Human TRPA1 Channel. 
  • Structural and functional analysis of the spontaneous re-formation of the thiol ester bond in human alpha 2-macroglobulin, rat alpha 1-inhibitor-3 and chemically modified derivatives. 
  • Structural characterization of the conformational change in calbindin-D28k upon calcium binding using differential surface modification analyzed by mass spectrometry. 
  • Substitution of an extracellular cysteine in the beta 2-adrenergic receptor enhances agonist-promoted phosphorylation and receptor desensitization. 
  • Sulfite oxidase deficiency. Biochemical and clinical investigations of a hereditary metabolic disorder in sulfur metabolism. 
  • Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro. 
  • Sulfoxidation of cysteine and mercapturic acid conjugates of the sevoflurane degradation product fluoromethyl-2,2-difluoro-1-(trifluoromethyl)vinyl ether (compound A). 
  • Synergistic cytotoxicity between menadione and dicumarol vs. murine leukemia L1210. 
  • Targeting RNA with cysteine-constrained peptides. 
  • Targeting the hexosamine biosynthetic pathway and O-linked N-acetylglucosamine cycling for therapeutic and imaging capabilities in diffuse large B-cell lymphoma. 
  • The disulfide bonding pattern in ficolin multimers. 
  • The immunosuppressant drug FTY720 inhibits cytosolic phospholipase A2 independently of sphingosine-1-phosphate receptors. 
  • The mechanism of discrimination between oxidized and reduced coenzyme in the aldehyde dehydrogenase domain of Aldh1l1. 
  • The nicotinic acetylcholine receptor gene family of the silkworm, Bombyx mori. 
  • The requirement of reversible cysteine sulfenic acid formation for T cell activation and function. 
  • The role of HMGB1 in the pathogenesis of inflammatory and autoimmune diseases. 
  • The staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers. 
  • The type I and type II bovine scavenger receptors expressed in Chinese hamster ovary cells are trimeric proteins with collagenous triple helical domains comprising noncovalently associated monomers and Cys83-disulfide-linked dimers. 
  • Transport rather than diffusion-dependent route for nitric oxide gas activity in alveolar epithelium. 
  • Transpulmonary flux of S-nitrosothiols and pulmonary vasodilation during nitric oxide inhalation: role of transport. 
  • Treatment of erythrocytes with the 2-cys peroxiredoxin inhibitor, Conoidin A, prevents the growth of Plasmodium falciparum and enhances parasite sensitivity to chloroquine. 
  • TrmB Family Transcription Factor as a Thiol-Based Regulator of Oxidative Stress Response. 
  • Ultrastructure and function of dimeric, soluble intercellular adhesion molecule-1 (ICAM-1). 
  • Variable presentation of nemaline myopathy: novel mutation of alpha actin gene. 
  • Visualizing multistep elevator-like transitions of a nucleoside transporter. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Gunn, Michael Dee, Professor of Medicine, Integrative Immunobiology
  • Hsu-Kim, Heileen, Professor in the Department of Civil and Environmental Engineering, Civil and Environmental Engineering
  • Preminger, Glenn Michael, James F. Glenn, M.D. Distinguished Professor of Urology, Surgery, Urology