Deoxyribonuclease I

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  Subject Areas on Research

  • A T cell-specific transcriptional enhancer within the human T cell receptor delta locus. 
  • A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion. 
  • A complex intronic enhancer regulates expression of the CFTR gene by direct interaction with the promoter. 
  • A genome-wide analysis of open chromatin in human tracheal epithelial cells reveals novel candidate regulatory elements for lung function. 
  • A novel, topologically constrained DNA molecule containing a double Holliday junction: design, synthesis, and initial biochemical characterization. 
  • Activation of TLX3 and NKX2-5 in t(5;14)(q35;q32) T-cell acute lymphoblastic leukemia by remote 3'-BCL11B enhancers and coregulation by PU.1 and HMGA1. 
  • An integrated encyclopedia of DNA elements in the human genome. 
  • Barriers to productive transfection of trabecular meshwork cells. 
  • Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications. 
  • Characterization of the DNase I hypersensitive site 3' of the human beta globin gene domain. 
  • Cloning and characterization of a novel endothelial promoter of the human CYP19 (aromatase P450) gene that is up-regulated in breast cancer tissue. 
  • Comprehensive analysis of the chromatin landscape in Drosophila melanogaster. 
  • Correlation of doxorubicin footprints with deletion endpoints in lacO of E. coli. 
  • DNase-chip: a high-resolution method to identify DNase I hypersensitive sites using tiled microarrays. 
  • DNase-seq predicts regions of rotational nucleosome stability across diverse human cell types. 
  • DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells. 
  • DNaseI hypersensitivity at gene-poor, FSH dystrophy-linked 4q35.2. 
  • DNase I sensitivity QTLs are a major determinant of human expression variation. 
  • Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases. 
  • Differential binding of c-Myc and Max to nucleosomal DNA. 
  • Differential staining of actin in metaphase spindles with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and fluorescent DNase: is actin involved in chromosomal movement? 
  • Diffusible factors are responsible for differences in nuclease sensitivity among chromatins originating from different cell types. 
  • Dissecting the regulatory architecture of gene expression QTLs. 
  • Distant conserved sequences flanking endothelial-specific promoters contain tissue-specific DNase-hypersensitive sites and over-represented motifs. 
  • Distinct sites on the G-actin molecule bind group-specific component and deoxyribonuclease I. 
  • Drosophila TFIID binds to a conserved downstream basal promoter element that is present in many TATA-box-deficient promoters. 
  • Enhancer-blocking activity within the DNase I hypersensitive site 2 to 6 region between the TCR alpha and Dad1 genes. 
  • Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold. 
  • Explicit DNase sequence bias modeling enables high-resolution transcription factor footprint detection. 
  • Expression of a yeast metallothionein gene family is activated by a single metalloregulatory transcription factor. 
  • Extensive evolutionary changes in regulatory element activity during human origins are associated with altered gene expression and positive selection. 
  • F-Seq: a feature density estimator for high-throughput sequence tags. 
  • Genome accessibility is widely preserved and locally modulated during mitosis. 
  • Genome-wide identification of regulatory elements in Sertoli cells. 
  • Genome-wide mapping of DNase hypersensitive sites using massively parallel signature sequencing (MPSS). 
  • Global epigenomic analysis of primary human pancreatic islets provides insights into type 2 diabetes susceptibility loci. 
  • Heritable individual-specific and allele-specific chromatin signatures in humans. 
  • High-resolution genome-wide in vivo footprinting of diverse transcription factors in human cells. 
  • High-resolution mapping and characterization of open chromatin across the genome. 
  • High-resolution mapping of open chromatin in the rice genome. 
  • Identification and characterization of cell type-specific and ubiquitous chromatin regulatory structures in the human genome. 
  • Identification of potassium-dependent and -independent components of the apoptotic machinery in mouse ovarian germ cells and granulosa cells. 
  • In situ nick translation of human and mouse chromosomes detected with a biotinylated nucleotide. 
  • In vitro interactions of CysB protein with the cysK and cysJIH promoter regions of Salmonella typhimurium. 
  • Mapping nucleosome positions using DNase-seq. 
  • Mapping regulatory elements by DNaseI hypersensitivity chip (DNase-Chip). 
  • Method for decellularizing skeletal muscle without detergents or proteolytic enzymes. 
  • Mispair specificity of methyl-directed DNA mismatch correction in vitro. 
  • Multiple single-stranded cis elements are associated with activated chromatin of the human c-myc gene in vivo. 
  • Nasally inhaled dornase alfa in the postoperative management of chronic sinusitis due to cystic fibrosis. 
  • Patterns of regulatory activity across diverse human cell types predict tissue identity, transcription factor binding, and long-range interactions. 
  • Peroxide-mediated chromatin remodelling of a nuclear factor kappa B site in the mouse inducible nitric oxide synthase promoter. 
  • Positive and negative elements regulate human interleukin 3 expression. 
  • Predicting cell-type-specific gene expression from regions of open chromatin. 
  • Rapid Detection of Urinary Tract Infections via Bacterial Nuclease Activity. 
  • Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae. 
  • Retroviral DNA integration: viral and cellular determinants of target-site selection. 
  • Revertants and partial transformants of rat fibroblasts infected with Fujinami sarcoma virus. 
  • Selective loss of a DNase I hypersensitive site upstream of the tyrosine aminotransferase gene in mice homozygous for lethal albino deletions. 
  • Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. 
  • Site-specific silencing of regulatory elements as a mechanism of X inactivation. 
  • Structural and transcriptional properties of different nucleosomal particles containing high mobility group proteins 14 and 17 (HMG 14/17). 
  • Suppression of hepatitis B virus DNA accumulation in chronically infected cells using a bacterial CRISPR/Cas RNA-guided DNA endonuclease. 
  • The SV40 core sequence functions as a repressor element in yeast. 
  • The Use of Poly-L-Lysine as a Capture Agent to Enhance the Detection of Antinuclear Antibodies by ELISA. 
  • The accessible chromatin landscape of the human genome. 
  • The compact chromatin structure of a Ty repeated sequence suppresses recombination hotspot activity in Saccharomyces cerevisiae. 
  • The forkhead transcription factor FoxI1 remains bound to condensed mitotic chromosomes and stably remodels chromatin structure. 
  • Transcriptional regulation of Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa: dissection of key promoter elements. 
  • Zeta-crystallin: a lens-specific promoter and the gene recruitment of an enzyme as a crystallin. 
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  Keywords of People

  • Krangel, Michael S., Mary Bernheim Distinguished Professor of Immunology, Immunology
  • Reddy, Timothy E, Associate Professor of Biostatistics & Bioinformatics, Molecular Genetics and Microbiology
  • Wray, Gregory Allan, Professor of Biology, Duke Innovation & Entrepreneurship