Disulfides

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  Subject Areas on Research

  • A geometric arrangement algorithm for structure determination of symmetric protein homo-oligomers from NOEs and RDCs. 
  • A randomized phase II study of PX-12, an inhibitor of thioredoxin in patients with advanced cancer of the pancreas following progression after a gemcitabine-containing combination. 
  • Activation of human plasminogen by urokinase. Partial characterization of a pre-activation peptide. 
  • Aldosterone increases oxidant stress to impair guanylyl cyclase activity by cysteinyl thiol oxidation in vascular smooth muscle cells. 
  • An analysis of type-C retrovirus polypeptides and their associations in the virion. 
  • Atomic distance estimates from disulfides and high-affinity metal-binding sites in a K+ channel pore. 
  • Broad Analysis of Vicinal Disulfides: Occurrences, Conformations with Cis or with Trans Peptides, and Functional Roles Including Sugar Binding. 
  • Characterization of fragment E from fibrinogen and cross-linked fibrin. 
  • Chemical cross-linking studies of beef heart mitochondrial coupling factor 1. 
  • Confirmation of the involvement of protein domain movement during the catalytic cycle of the cytochrome bc1 complex by the formation of an intersubunit disulfide bond between cytochrome b and the iron-sulfur protein. 
  • Conformational dynamics of amyloid beta-protein assembly probed using intrinsic fluorescence. 
  • Constitutive activation of the N-methyl-D-aspartate receptor via cleft-spanning disulfide bonds. 
  • Construction of a novel redox protein by rational design: conversion of a disulfide bridge into a mononuclear iron-sulfur center. 
  • Crystal structure of a pol alpha family DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. 9 degrees N-7. 
  • Delivery of antisense oligonucleotides using HPMA polymer: synthesis of A thiol polymer and its conjugation to water-soluble molecules. 
  • Detection and characterization of methionine oxidation in peptides by collision-induced dissociation and electron capture dissociation. 
  • Determinants of backbone dynamics in native BPTI: cooperative influence of the 14-38 disulfide and the Tyr35 side-chain. 
  • Determination and analysis of the 2 A-structure of copper, zinc superoxide dismutase. 
  • Discovery of high-affinity peptide binders to BLyS by phage display. 
  • Disulfide bond requirements for active Wnt ligands. 
  • Disulfide-mediated dimerization of L1 Ig domains. 
  • Effect of oxidative stress on homer scaffolding proteins. 
  • Engineered chimeric streptavidin tetramers as novel tools for bioseparations and drug delivery. 
  • Erythropoietin structure-function relationships. Mutant proteins that test a model of tertiary structure. 
  • Evidence for the intertwined dimer of the cytochrome bc(1) complex in solution. 
  • Expression of human T cell receptor-gamma delta structural forms. 
  • Fibronectin aggregation and assembly: the unfolding of the second fibronectin type III domain. 
  • Functional properties of hemoglobin Pôrto Alegre (alpha2A beta2 9Ser leads to Cys) and the reactivity of its extra cysteinyl residue. 
  • Gadolinium-conjugated gold nanoshells for multimodal diagnostic imaging and photothermal cancer therapy. 
  • Generation and Characterization of a Bivalent HIV-1 Subtype C gp120 Protein Boost for Proof-of-Concept HIV Vaccine Efficacy Trials in Southern Africa. 
  • Homocysteinylated fibrinogen forms disulfide-linked complexes with albumin. 
  • Interaction sites of the C-terminal region of the cGMP phosphodiesterase inhibitory subunit with the GDP-bound transducin alpha-subunit. 
  • Internal triplication in the structure of human ceruloplasmin. 
  • Isolation and biochemical characterization of LEAP-2, a novel blood peptide expressed in the liver. 
  • Isolation and structures of novel fungal metabolites as chemokine receptor (CCR2) antagonists. 
  • Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins. 
  • Modification of cysteine 111 in human Cu,Zn-superoxide dismutase. 
  • Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation. 
  • NMR-derived solution conformations of a hybrid synthetic peptide containing multiple epitopes of envelope protein gp120 from the RF strain of human immunodeficiency virus. 
  • Nitroxyl disulfides, novel intermediates in transnitrosation reactions. 
  • Novel disulfide engineering in human carbonic anhydrase II using the PAIRWISE side-chain geometry database. 
  • Oligomeric structure and tissue distribution of ficolins from mouse, pig and human. 
  • Optimization of the Solubility of HIV-1-Neutralizing Antibody 10E8 through Somatic Variation and Structure-Based Design. 
  • Oxidation-induced intramolecular disulfide bond inactivates mitogen-activated protein kinase kinase 6 by inhibiting ATP binding. 
  • Pathways of folding of reduced bovine pancreatic ribonuclease. 
  • Poly(ethylene glycol) hydrogels with cell cleavable groups for autonomous cell delivery. 
  • Principal neutralizing domain of the human immunodeficiency virus type 1 envelope protein. 
  • Protein polymer hydrogels by in situ, rapid and reversible self-gelation. 
  • Psammaplin A as a general activator of cell-based signaling assays via HDAC inhibition and studies on some bromotyrosine derivatives. 
  • Pungent products from garlic activate the sensory ion channel TRPA1. 
  • Recombinant immunotoxin containing a disulfide-stabilized Fv directed at erbB2 that does not require proteolytic activation. 
  • Redox Activation of Nox1 (NADPH Oxidase 1) Involves an Intermolecular Disulfide Bond Between Protein Disulfide Isomerase and p47phox in Vascular Smooth Muscle Cells. 
  • Role of extracellular disulfide-bonded cysteines in the ligand binding function of the beta 2-adrenergic receptor. 
  • Secretion of extracellular superoxide dismutase in neonatal lungs. 
  • Stabilization of HIV-1 envelope in the CD4-bound conformation through specific cross-linking of a CD4 mimetic. 
  • Stabilizing exposure of conserved epitopes by structure guided insertion of disulfide bond in HIV-1 envelope glycoprotein. 
  • Structural Basis of WLS/Evi-Mediated Wnt Transport and Secretion. 
  • Structural characterization of the conformational change in calbindin-D28k upon calcium binding using differential surface modification analyzed by mass spectrometry. 
  • Structural mapping of chloroplast coupling factor. 
  • Structure and folding of nascent polypeptide chains during protein translocation in the endoplasmic reticulum. 
  • Structure determination of human and murine beta-defensins reveals structural conservation in the absence of significant sequence similarity. 
  • The RGD motif in fibronectin is essential for development but dispensable for fibril assembly. 
  • The chemokines IL-8, monocyte chemoattractant protein-1, and I-309 are monomers at physiologically relevant concentrations. 
  • The disulfide bonding pattern in ficolin multimers. 
  • The release of acid phosphatase and polysaccharide- and protein-containing components from the surface of the dimorphic forms of Candida albicans by treatment with dithiothreitol. 
  • The staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers. 
  • The toxin-agglutinin fold. A new group of small protein structures organized around a four-disulfide core. 
  • The type I and type II bovine scavenger receptors expressed in Chinese hamster ovary cells are trimeric proteins with collagenous triple helical domains comprising noncovalently associated monomers and Cys83-disulfide-linked dimers. 
  • Thermodynamic effects of reduction of the active-site disulfide of Escherichia coli thioredoxin explored by differential scanning calorimetry. 
  • TrmB Family Transcription Factor as a Thiol-Based Regulator of Oxidative Stress Response. 
  • Two forms of the T-cell receptor gamma protein found on peripheral blood cytotoxic T lymphocytes. 
  • Two separate interfaces between the voltage sensor and pore are required for the function of voltage-dependent K(+) channels. 
  • Tyrosine phosphorylation is required for functional activation of disulfide-containing constitutively active STAT mutants. 
  • [3H]acetylcholine binding sites in brain. Effect of disulfide bond modification. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology