DNA Polymerase I

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  Subject Areas on Research

  • Arrest of replication by mammalian DNA polymerases alpha and beta caused by chromium-DNA lesions. 
  • Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites. 
  • Chromosome aberrations resulting from double-strand DNA breaks at a naturally occurring yeast fragile site composed of inverted ty elements are independent of Mre11p and Sae2p. 
  • Cocrystal structure of an editing complex of Klenow fragment with DNA. 
  • Combinatorial gene control involving E2F and E Box family members. 
  • Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. 
  • Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate. 
  • Cut5 is required for the binding of Atr and DNA polymerase alpha to genotoxin-damaged chromatin. 
  • Discrimination between right and wrong purine dNTPs by DNA polymerase I from Bacillus stearothermophilus. 
  • Dissecting the differences between the alpha and beta anomers of the oxidative DNA lesion FaPydG. 
  • Elevated DNA polymerase alpha, DNA polymerase beta, and DNA topoisomerase II in a melphalan-resistant rhabdomyosarcoma xenograft that is cross-resistant to nitrosoureas and topotecan. 
  • Enhanced repair of a cisplatin-damaged reporter chloramphenicol-O-acetyltransferase gene and altered activities of DNA polymerases alpha and beta, and DNA ligase in cells of a human malignant glioma following in vivo cisplatin therapy. 
  • Enhancement of melphalan activity by inhibition of DNA polymerase-alpha and DNA polymerase-beta. 
  • Following an environmental carcinogen N2-dG adduct through replication: elucidating blockage and bypass in a high-fidelity DNA polymerase. 
  • Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. 
  • Heterogeneous polymerase fidelity and mismatch repair bias genome variation and composition. 
  • Identification of E-box factor TFE3 as a functional partner for the E2F3 transcription factor. 
  • Introduction of the alpha-P-borano-group into deoxynucleoside triphosphates increases their selectivity to HIV-1 reverse transcriptase relative to DNA polymerases. 
  • Isolation and characterization of the replicon of a Thiobacillus intermedius plasmid. 
  • Low levels of DNA polymerase alpha induce mitotic and meiotic instability in the ribosomal DNA gene cluster of Saccharomyces cerevisiae. 
  • Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication. 
  • Mechanisms of DNA-mismatch correction. 
  • Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations. 
  • Purification and properties of DNA polymerase-beta from guinea pig liver. 
  • Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain. 
  • Structural basis for the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism. 
  • Structural studies of Klenow fragment: an enzyme with two active sites. 
  • Structure of DNA polymerase I Klenow fragment bound to duplex DNA. 
  • The Closing Mechanism of DNA Polymerase I at Atomic Resolution. 
  • The mechanism of the translocation step in DNA replication by DNA polymerase I: a computer simulation analysis. 
  • The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an "ajar" intermediate conformation in the nucleotide selection mechanism. 
  • Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes. 
  • Two DNA polymerases: HIV reverse transcriptase and the Klenow fragment of Escherichia coli DNA polymerase I. 
  • Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal.