Drug Stability

• 

  Subject Areas on Research

  • A high affinity, antidote-controllable prothrombin and thrombin-binding RNA aptamer inhibits thrombin generation and thrombin activity. 
  • A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma. 
  • A nuclear magnetic resonance study of the interaction of L-epinephrine with phospholipid vesicles. 
  • A trithiol bifunctional chelate for 72,77As: A matched pair theranostic complex with high in vivo stability. 
  • Aggregation of rabbit muscle phosphofructokinase. 
  • Analysis of the plasma elimination kinetics and conformational stabilities of native, proteinase-complexed, and reactive site cleaved serpins: comparison of alpha 1-proteinase inhibitor, alpha 1-antichymotrypsin, antithrombin III, alpha 2-antiplasmin, angiotensinogen, and ovalbumin. 
  • Anti-AIDS agents 90. novel C-28 modified bevirimat analogues as potent HIV maturation inhibitors. 
  • Anti-AIDS agents. 78. Design, synthesis, metabolic stability assessment, and antiviral evaluation of novel betulinic acid derivatives as potent anti-human immunodeficiency virus (HIV) agents. 
  • Anticolon cancer activity of largazole, a marine-derived tunable histone deacetylase inhibitor. 
  • Assembly of microtubules from preformed, ring-shaped protofilaments and 6-S tubulin. 
  • Assessing distributions of estimated drug shelf lives in stability analysis. 
  • Assessing patients' and caregivers' perspectives on stability of factor VIII products for haemophilia A: a web-based study in the United States and Canada. 
  • Astatination of proteins using an N-succinimidyl tri-n-butylstannyl benzoate intermediate. 
  • Astatine-211 labeling of internalizing anti-EGFRvIII monoclonal antibody using N-succinimidyl 5-[211At]astato-3-pyridinecarboxylate. 
  • Biological evaluation of ring- and side-chain-substituted m-iodobenzylguanidine analogues. 
  • Characterization of Mn(III) complexes of linear and cyclic desferrioxamines as mimics of superoxide dismutase activity. 
  • Characterization of a microcarrier cell culture system for 23Na MR spectroscopy studies. 
  • Characterization of the aqueous iron(III) chelation chemistry of a potential Trojan Horse antimicrobial agent: chelate structure, stability and pH dependent speciation. 
  • Chromatographic analysis and decomposition product characterization of compound SR16157 (NSC 732011). 
  • Comparative analyses of mechanistic differences among antiestrogens. 
  • Compatibility and stability of bryostatin 1 in infusion devices. 
  • Compatibility of aminophylline and verapamil in intravenous admixtures. 
  • Copper Influences the Antibacterial Outcomes of a β-Lactamase-Activated Prochelator against Drug-Resistant Bacteria. 
  • Cytosolic delivery of macromolecules: I. Synthesis and characterization of pH-sensitive acyloxyalkylimidazoles. 
  • DNA synthesis in strains of Escherichia coli K12 with temperature-sensitive DNA ligase and DNA polymerase I. 
  • Destruction of Myc by ubiquitin-mediated proteolysis: cancer-associated and transforming mutations stabilize Myc. 
  • Determination of high density lipoprotein-cholesterol in stored human plasma. 
  • Determination of total folate in plant material by chemical conversion into para-aminobenzoic acid followed by high performance liquid chromatography combined with on-line postcolumn derivatization and fluorescence detection. 
  • Development of a liquid chromatography-tandem mass spectrometry assay of six antimicrobials in plasma for pharmacokinetic studies in premature infants. 
  • Development of lipophilic anticancer agents for the treatment of brain tumors by the esterification of water-soluble chlorambucil. 
  • Direct liquid chromatography determination of the reactive imine SJG-136 (NSC 694501). 
  • Directed intermixing in multicomponent self-assembling biomaterials. 
  • Domain interactions in oxidized and reduced forms of rat liver sulfite oxidase. 
  • Effect of heme and non-heme ligands on subunit dissociation of normal and carboxypeptidase-digested hemoglobin. Gel filtration and flash photolysis studies. 
  • Effect of oxygen and allosteric effects on structural stability of oligomeric hemocyanins of the arthropod, Limulus polyphemus, and the mollusc, Helix pomatia. 
  • Effect of pigmentation on the mechanical and polymerization characteristics of bone cement. 
  • Emergent bistability by a growth-modulating positive feedback circuit. 
  • Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. 
  • Estimating drug shelf-life with random batches. 
  • Evaluation of preanalytical variables associated with measurement of prothrombin fragment 1.2. 
  • Ferulic acid stabilizes a solution of vitamins C and E and doubles its photoprotection of skin. 
  • Functional analysis of the lipoglycodepsipeptide antibiotic ramoplanin. 
  • Functional domain and motif analyses of androgen receptor coregulator ARA70 and its differential expression in prostate cancer. 
  • Gamma-L-glutaminyl-3,4-benzoquinone. Structural studies and enzymatic synthesis. 
  • Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein. 
  • Genetically stable picornavirus expression vectors with recombinant internal ribosomal entry sites. 
  • Glutamine stability in biological tissues evaluated by fluorometric analysis. 
  • Growth and lability of Chaetopterus oocyte mitotic spindles isolated in the presence of porcine brain tubulin. 
  • Hierarchical Self-Assembly of Noncovalent Amphiphiles 
  • High performance liquid chromatography-mass spectrometry assay for polymyxin B1 and B2 in human plasma. 
  • High-sensitivity analysis of buprenorphine, norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide in plasma and urine by liquid chromatography-mass spectrometry. 
  • Human IgG2 constant region enhances in vivo stability of anti-tenascin antibody 81C6 compared with its murine parent. 
  • Human interleukin-2 production in insect (Trichoplusia ni) larvae: effects and partial control of proteolysis. 
  • In vitro and in vivo evaluation of a water-in-oil microemulsion system for enhanced peptide intestinal delivery. 
  • In vitro circuit stability of 5-fluorouracil and oxaliplatin in support of hyperthermic isolated hepatic perfusion. 
  • Influence of allosteric ligands on the activity and aggregation of rabbit muscle phosphofructokinase. 
  • Inhibition of nuclear factor-kappaB-mediated adhesion molecule expression in human endothelial cells. 
  • Instability of myelin tubes under dehydration: deswelling of layered cylindrical structures. 
  • Investigation of the extraction behavior of the main monoglutamate folates from spinach by liquid chromatography-electrospray ionization tandem mass spectrometry. 
  • LC-UV/MS methods for the analysis of prochelator-boronyl salicylaldehyde isonicotinoyl hydrazone (BSIH) and its active chelator salicylaldehyde isonicotinoyl hydrazone (SIH). 
  • Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability. 
  • Lactate measured in diluted and undiluted whole blood and plasma: comparison of methods and effect of hematocrit. 
  • Long-residence-time nano-scale liposomal iohexol for X-ray-based blood pool imaging. 
  • Measurement of felbamate by wide-bore capillary gas chromatography and flame ionization detection. 
  • Metal-Organic Framework Encapsulation Preserves the Bioactivity of Protein Therapeutics. 
  • N-succinimidyl 4-methyl-3-(tri-n-butylstannyl)benzoate: synthesis and potential utility for the radioiodination of monoclonal antibodies. 
  • N-succinimidyl 5-(trialkylstannyl)-3-pyridinecarboxylates: a new class of reagents for protein radioiodination. 
  • Neutrophil elastase increases MUC5AC mRNA and protein expression in respiratory epithelial cells. 
  • Nitric oxide binding to human ferrihemoglobins cross-linked between either alpha or beta subunits. 
  • Novel dry powder preparations of whole inactivated influenza virus for nasal vaccination. 
  • On stability designs in drug shelf-life estimation. 
  • Optimization of 2,4-diarylanilines as non-nucleoside HIV-1 reverse transcriptase inhibitors. 
  • Paired-ion liquid chromatographic method for the analysis of a phenanthrenemethanol antimalarial in whole blood. 
  • Phospholipid-dextran with a single coupling point: a useful amphiphile for functionalization of nanomaterials. 
  • Preparation and biological evaluation of an astatine-211 labeled biotin conjugate: biotinyl-3-[211 At]astatoanilide. 
  • Preparation and characterization of anti-tenascin monoclonal antibody-streptavidin conjugates for pretargeting applications. 
  • Preparation of 5-[131I]iodo- and 5-[211At]astato-1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl) uracil by a halodestannylation reaction. 
  • Production and quality control assessment of a GLP-grade immunotoxin, D2C7-(scdsFv)-PE38KDEL, for a phase I/II clinical trial. 
  • Proteinase activity and stability of natural bromelain preparations. 
  • Purification and properties of DNA polymerase-beta from guinea pig liver. 
  • Purification and properties of the NAD+-dependent (type D) and O2-dependent (type O) forms of rat liver xanthine dehydrogenase. 
  • Rapid degradation of puromycyl peptides in hepatoma cells and reticulocytes. 
  • Reagents for astatination of biomolecules: comparison of the in vivo distribution and stability of some radioiodinated/astatinated benzamidyl and nido-carboranyl compounds. 
  • Relationship of sialic acid to function and in vivo survival of human factor VIII/von Willebrand factor protein. 
  • S-N dissociation energies of S-nitrosothiols: on the origins of nitrosothiol decomposition rates. 
  • S-Nitrosohemoglobin is unstable in the reductive erythrocyte environment and lacks O2/NO-linked allosteric function. 
  • Sevoflurane formulation water content influences degradation by Lewis acids in vaporizers. 
  • Signal discrimination by differential regulation of protein stability in quorum sensing. 
  • Simultaneous determination of alfentanil and midazolam in human plasma using liquid chromatography and tandem mass spectrometry. 
  • Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS. 
  • Smoking of phencyclidine: disposition in man and stability to pyrolytic conditions. 
  • Solid-phase microtiter radioimmunoassay blocking test for detection of antibodies to Escherichia coli heat-labile enterotoxin. 
  • Spermine dialdehyde, a novel ex vivo purging agent for both allogenic and autologous bone marrow transplantations. 
  • Stability analysis for drugs with multiple active ingredients. 
  • Stability analysis with discrete responses. 
  • Stability and in vivo behavior of Rh[16aneS4-diol]211 at complex: a potential precursor for astatine radiopharmaceuticals. 
  • Stability of fibrin sealant in cerebrospinal fluid: an in vitro study. 
  • Stability of oxidized Escherichia coli thioredoxin and its dependence on protonation of the aspartic acid residue in the 26 position. 
  • Stability of recombinant tissue plasminogen activator. 
  • Stability-indicating LC assay of and impurity identification in homoharringtonine samples. 
  • Stable dry powder formulation for nasal delivery of anthrax vaccine. 
  • Structure and stability of monomeric lambda repressor: NMR evidence for two-state folding. 
  • Studies on the dissociation of flavin adenine dinucleotide from metalloflavoproteins. 
  • Synthesis and preliminary biological evaluation of (3-iodobenzoyl)norbiotinamide and ((5-iodo-3-pyridinyl)carbonyl)norbiotinamide: two radioiodinated biotin conjugates with improved stability. 
  • Synthesis, Hydrolysis, and Protonation-Promoted Intramolecular Reductive Breakdown of Potential NRTIs: Stavudine α-P-Borano-γ-P-N-l-tryptophanyltriphosphates. 
  • Synthesis, purification, and in vitro stability of 211At- and 125I-labeled amidobisphosphonates. 
  • Targeting a genetically engineered elastin-like polypeptide to solid tumors by local hyperthermia. 
  • Test for batch-to-batch variation in stability analysis. 
  • Test for batch-to-batch variation in stability analysis. 
  • The decomposition of thionitrites. 
  • The international standard for atrial natriuretic factor. Calibration by an international collaborative study. 
  • The materials engineering of temperature-sensitive liposomes. 
  • The rational design and development of a dual chamber vaginal/rectal microbicide gel formulation for HIV prevention. 
  • Thermal stability of the prototypical Mn porphyrin-based superoxide dismutase mimic and potent oxidative-stress redox modulator Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin chloride, MnTE-2-PyP(5+). 
  • Tracking the in vivo fate of recombinant polypeptides by isotopic labeling. 
  • Treatment of iron deficiency anemia: are monomeric iron compounds suitable for parenteral administration? 
  • Trithiols and their arsenic compounds for potential use in diagnostic and therapeutic radiopharmaceuticals. 
  • Two-phase shelf-life estimation. 
  • Using DNA duplex stability information for transcription factor binding site discovery. 
  • Vaccine storage practices in primary care physician offices: assessment and intervention. 
  • Vaginal drug distribution modeling. 
  • Zinc acetate/carrageenan gels exhibit potent activity in vivo against high-dose herpes simplex virus 2 vaginal and rectal challenge. 
• 

  Keywords of People

  • Cohen-Wolkowiez, Michael, Kiser-Arena Distinguished Professor, Pediatrics, Infectious Diseases
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology