Enzyme Stability

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  Subject Areas on Research

  • A novel thermostable branching enzyme from an extremely thermophilic bacterial species, Rhodothermus obamensis. 
  • A toggle duplex in hepatitis delta virus self-cleaving RNA that stabilizes an inactive and a salt-dependent pro-active ribozyme conformation. 
  • A unique protein kinase C-dependent pathway for tissue factor downregulation in pericytes. 
  • Activated c-Abl is degraded by the ubiquitin-dependent proteasome pathway. 
  • Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5'-phosphosulfate kinase. 
  • Competing Pathways and Multiple Folding Nuclei in a Large Multidomain Protein, Luciferase. 
  • Complexes between serpins and inactive proteinases are not thermodynamically stable but are recognized by serpin receptors. 
  • Computational design of a biologically active enzyme. 
  • Converting a maltose receptor into a nascent binuclear copper oxygenase by computational design. 
  • Crystal structure of a pol alpha family DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. 9 degrees N-7. 
  • Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. 
  • Crystallization and preliminary X-ray crystallographic investigations on several thermostable forms of a Bacillus subtilis lipase. 
  • Cyclophilin A and Ess1 interact with and regulate silencing by the Sin3-Rpd3 histone deacetylase. 
  • Difference between mitochondrial RNase P and nuclear RNase P. 
  • Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor. 
  • Effect of temperature, pH, and metals on the stability and activity of phenylalanine hydroxylase from Chromobacterium violaceum. 
  • Function of the hydrophilic carboxyl terminus of type II DNA topoisomerase from Drosophila melanogaster. I. In vitro studies. 
  • In vivo and in vitro examination of stability of primary hyperoxaluria-associated human alanine:glyoxylate aminotransferase. 
  • Inhibitor binding increases the mechanical stability of staphylococcal nuclease. 
  • Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype. 
  • MAPK-activated protein kinase-2 in cardiac hypertrophy and cyclooxygenase-2 regulation in heart. 
  • Mapping staphylococcal nuclease conformation using an EDTA-Fe derivative attached to genetically engineered cysteine residues. 
  • Measurements of protein stability by H/D exchange and matrix-assisted laser desorption/ionization mass spectrometry using picomoles of material. 
  • Molecular basis for paradoxical carriers of adenosine deaminase (ADA) deficiency that show extremely low levels of ADA activity in peripheral blood cells without immunodeficiency. 
  • Molecular cloning, characterization, and expression in Escherichia coli of full-length cDNAs of three human glutathione S-transferase Pi gene variants. Evidence for differential catalytic activity of the encoded proteins. 
  • Most mutations that cause spinocerebellar ataxia autosomal recessive type 16 (SCAR16) destabilize the protein quality-control E3 ligase CHIP. 
  • Mutational analysis of active site residues in the Staphylococcus aureus transpeptidase SrtA. 
  • NMR structure of activated CheY. 
  • Phosphorylation of RNA polymerase II CTD fragments results in tight binding to the WW domain from the yeast prolyl isomerase Ess1. 
  • Proteases of Dermatophagoides pteronyssinus. 
  • Proteinase activity and stability of natural bromelain preparations. 
  • Regulation of mitochondrial morphology by APC/CCdh1-mediated control of Drp1 stability. 
  • Role of enzyme-bound 5,10-methenyltetrahydropteroylpolyglutamate in catalysis by Escherichia coli DNA photolyase. 
  • Stability studies on a lipase from Bacillus subtilis in guanidinium chloride. 
  • Structural basis for the remarkable stability of Bacillus subtilis lipase (Lip A) at low pH. 
  • Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase. 
  • Structure of the molybdenum site of Rhodobacter sphaeroides biotin sulfoxide reductase. 
  • Substitution of cadmium for zinc in farnesyl:protein transferase alters its substrate specificity. 
  • Sustained delivery of thermostabilized chABC enhances axonal sprouting and functional recovery after spinal cord injury. 
  • Targeting specific PDZ domains of PSD-95; structural basis for enhanced affinity and enzymatic stability of a cyclic peptide. 
  • The hyperthermophilic bacterium Thermotoga neapolitana possesses two isozymes of the 3-phosphoglycerate kinase/triosephosphate isomerase fusion protein. 
  • The thermolabile variant of methylenetetrahydrofolate reductase (MTHFR) is not a major risk factor for neural tube defect in American Caucasians. The NTD Collaborative Group. 
  • Thermodynamic analysis of protein stability and ligand binding using a chemical modification- and mass spectrometry-based strategy. 
  • Thermodynamic analysis of subunit interactions in Escherichia coli molybdopterin synthase. 
  • Thermodynamic stability measurements on multimeric proteins using a new H/D exchange- and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry-based method. 
  • Unraveling the Mechanical Unfolding Pathways of a Multidomain Protein: Phosphoglycerate Kinase. 
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  Keywords of People

  • Oas, Terrence Gilbert, Professor of Biochemistry, Biochemistry