Fluorescence

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  Subject Areas on Research

  • A chimeric kinesin-1 head/kinesin-5 tail motor switches between diffusive and processive motility. 
  • A domain in TNF receptors that mediates ligand-independent receptor assembly and signaling. 
  • A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome. 
  • A kinetic study of the binding of an ADP fluorescent analog to mitochondrial ATPase. 
  • A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom. 
  • A nonradioactive assay for transfected chloramphenicol acetyltransferase activity using fluorescent substrates. 
  • A novel imaging system permits real-time in vivo tumor bed assessment after resection of naturally occurring sarcomas in dogs. 
  • A plant lipocalin promotes retinal-mediated oscillatory lateral root initiation. 
  • A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice. 
  • Admission cell free DNA levels predict 28-day mortality in patients with severe sepsis in intensive care. 
  • Age-dependent retinal iron accumulation and degeneration in hepcidin knockout mice. 
  • An Adaptive Real-Time 3D Single Particle Tracking Method for Monitoring Viral First Contacts. 
  • An electrophysiological study of the forebrain projection of nucleus commissuralis: preliminary identification of presumed A2 catecholaminergic neurons. 
  • An experimental "trans-molybdenum" tube for mammography. 
  • Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity. 
  • Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection. 
  • Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. 
  • Autofluorescence of choroidal melanoma in 51 cases. 
  • Autofluorescence of choroidal nevus in 64 cases. 
  • Autofluorescence of orange pigment overlying small choroidal melanoma. 
  • BiPS, a photocleavable, isotopically coded, fluorescent cross-linker for structural proteomics. 
  • Binding and signaling of surface-immobilized reagentless fluorescent biosensors derived from periplasmic binding proteins. 
  • CLARITY and PACT-based imaging of adult zebrafish and mouse for whole-animal analysis of infections. 
  • Capillary electrophoresis/laser-induced fluorescence detection of fluorescein as a groundwater migration tracer. 
  • Cardiomyocyte cultures with controlled macroscopic anisotropy: a model for functional electrophysiological studies of cardiac muscle. 
  • Cephalopod-inspired design of electro-mechano-chemically responsive elastomers for on-demand fluorescent patterning. 
  • Characterization of COMMD protein-protein interactions in NF-kappaB signalling. 
  • Characterization of nucleotide binding sites on chloroplast coupling factor 1. 
  • Cholesterol interaction with the daunorubicin binding site of P-glycoprotein. 
  • Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies. 
  • Comparative analysis of circulating endothelial progenitor cells in age-related macular degeneration patients using automated rare cell analysis (ARCA) and fluorescence activated cell sorting (FACS). 
  • Constitutive cytokine mRNAs mark natural killer (NK) and NK T cells poised for rapid effector function. 
  • Construction and detection of fluorescent, germline transgenic zebrafish. 
  • Conversion of a maltose receptor into a zinc biosensor by computational design. 
  • Coordination of replication and transcription along a Drosophila chromosome. 
  • Creation of an Automated Fluorescence Guided Tumor Ablation System. 
  • Design and Analysis of Localized DNA Hybridization Chain Reactions 
  • Detection of autofluorescent Mycobacterium chelonae in living zebrafish. 
  • Determination of intracellular species at the level of a single erythrocyte via capillary electrophoresis with direct and indirect fluorescence detection. 
  • Development of a fluorescence detection system using optical parametric oscillator (OPO) laser excitation for in vivo diagnosis. 
  • Development of a fluorescent ligand-binding assay using the AcroWell filter plate. 
  • Differential retention of rhodamine 123 by avian sarcoma virus-induced glioma and normal brain tissue of the rat in vivo. 
  • Diffuse optical monitoring of hemodynamic changes in piglet brain with closed head injury. 
  • Direct in vivo measurement of targeted binding in a human tumor xenograft. 
  • Discovery of Thermostable, Fluorescently Responsive Glucose Biosensors by Structure-Assisted Function Extrapolation. 
  • Do inner shells of double-walled carbon nanotubes fluoresce? 
  • Effects of potassium on oxidative metabolism and seizures. 
  • Effects of pulse strength and pulse duration on in vitro DNA electromobility. 
  • EndoU is a novel regulator of AICD during peripheral B cell selection. 
  • Enhanced postischemic functional recovery in CYP2J2 transgenic hearts involves mitochondrial ATP-sensitive K+ channels and p42/p44 MAPK pathway. 
  • Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2. 
  • Flow cytometric analysis of cell surface carbohydrates in metastatic human breast cancer. 
  • Fluorescence as a means of colour signal enhancement. 
  • Fluorescence changes on contractile activation in TnC(DANZ) labeled skinned rabbit psoas fibers. 
  • Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1. 
  • Fluorescence energy transfer between the thiamine diphosphate and flavine adenine dinucleotide binding sites on the pyruvate dehydrogenase multienzyme complex. 
  • Fluorescence-based alternative splicing reporters for the study of epithelial plasticity in vivo. 
  • Fluorescence-based measurement of cystine uptake through xCT shows requirement for ROS detoxification in activated lymphocytes. 
  • Fluorescence-based sorting of Caenorhabditis elegans via acoustofluidics. 
  • Fluorescent epibatidine agonists for neuronal and muscle-type nicotinic acetylcholine receptors. 
  • Folding dynamics of phenylalanine hydroxylase depends on the enzyme's metallation state: the native metal, iron, protects against aggregate intermediates. 
  • Formulations which increase the size of lipoplexes prevent serum-associated inhibition of transfection. 
  • Functional reconstitution of purified human Hv1 H+ channels. 
  • Fundus autofluorescence imaging in age-related macular degeneration and geographic atrophy. 
  • Fura-2 transport in toad urinary bladder epithelium: effects of antidiuretic hormone, colchicine and osmotic gradients. 
  • Gold Nanorod Size-Dependent Fluorescence Enhancement for Ultrasensitive Fluoroimmunoassays. 
  • High-resolution in vivo imaging of fluorescent proteins using window chamber models. 
  • Hydrogen sulphide enhances photosynthesis through promoting chloroplast biogenesis, photosynthetic enzyme expression, and thiol redox modification in Spinacia oleracea seedlings. 
  • Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates. 
  • Immobilized molecular beacons: a new strategy using UV-activated poly(methyl methacrylate) surfaces to provide large fluorescence sensitivities for reporting on molecular association events. 
  • Improved speciation of dissolved organic nitrogen in natural waters: amide hydrolysis with fluorescence derivatization. 
  • In vivo bone lead measurements: a rapid monitoring method for cumulative lead exposure. 
  • In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices. 
  • In vivo dendritic cell tracking using fluorescence lifetime imaging and near-infrared-emissive polymersomes. 
  • In vivo fluorescein staining of SI-30NB silicone intraocular lens. 
  • Indocyanine Green Fluorescence Imaging with Lymphoscintigraphy for Sentinel Node Biopsy in Melanoma: Increasing the Sentinel Lymph Node-Positive Rate. 
  • Influence of metal ions on substrate binding and catalytic activity of mammalian protein geranylgeranyltransferase type-I. 
  • Intensive care unit environmental cleaning: an evaluation in sixteen hospitals using a novel assessment tool. 
  • Interleukin-1 and tumor necrosis factor alpha inhibit repair of the porcine meniscus in vitro. 
  • Internal trafficking and surface mobility of a functionally intact beta2-adrenergic receptor-green fluorescent protein conjugate. 
  • LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching. 
  • LOCALIZATION OF IMMUNOGLOBULINS IN CHRONIC THYROIDITIS. 
  • Laser scanning cytometry quantification of estrogen receptors in breast cancer. 
  • Laser-induced fluorescence for esophageal cancer and dysplasia diagnosis. 
  • Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution. 
  • Linking PBDEs in house dust to consumer products using X-ray fluorescence. 
  • Localization of heparin cofactor II in injured human skin: a potential role in wound healing. 
  • Mapping Hfq-RNA interaction surfaces using tryptophan fluorescence quenching. 
  • Metal-enhanced fluorescence of carbon nanotubes. 
  • Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish. 
  • Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissues. 
  • NADH fluorescence and [K+]o changes during hippocampal electrical stimulation. 
  • Near-infrared-fluorescence-enhanced molecular imaging of live cells on gold substrates. 
  • Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators. 
  • Noncovalent functionalization of carbon nanotubes by fluorescein-polyethylene glycol: supramolecular conjugates with pH-dependent absorbance and fluorescence. 
  • Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe. 
  • Optical and pharmacological tools to investigate the role of mitochondria during oxidative stress and neurodegeneration. 
  • Optimal visual perception and detection of oral cavity neoplasia. 
  • Oxygen sufficiency in working rabbit papillary muscle at 35 degrees C. 
  • Phenylalanine-stimulated secretion of cholecystokinin is calcium dependent. 
  • Post-fabrication QAC-functionalized thermoplastic polyurethane for contact-killing catheter applications. 
  • Probing for Binding Regions of the FtsZ Protein Surface through Site-Directed Insertions: Discovery of Fully Functional FtsZ-Fluorescent Proteins. 
  • Psychedelic-inspired drug discovery using an engineered biosensor. 
  • Quantifying lipofuscin in retinal pigment epithelium in vivo by visible-light optical coherence tomography-based multimodal imaging. 
  • Quantitative comparison of algorithms for tracking single fluorescent particles. 
  • Quantum confined peptide assemblies with tunable visible to near-infrared spectral range. 
  • Rapid and orthogonal logic gating with a gibberellin-induced dimerization system. 
  • Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. 
  • Rapid in vitro assembly of Caulobacter crescentus FtsZ protein at pH 6.5 and 7.2. 
  • Rapid nanoimprinting of doped silk films for enhanced fluorescent emission. 
  • Regulation of G protein-coupled receptor kinase 5 (GRK5) by actin. 
  • Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins. 
  • Responses of cultured rat trigeminal ganglion neurons to bitter tastants. 
  • Reticular drusen associated with geographic atrophy in age-related macular degeneration. 
  • S1-nuclease enhancement of the ethidium bromide binding assay of drug-induced DNA interstrand crosslinking in human brain tumor cells. 
  • Sequence determinants of in cell condensate morphology, dynamics, and oligomerization as measured by number and brightness analysis. 
  • Simultaneous measurement of liposome extravasation and content release in tumors. 
  • Simultaneous monitoring of tissue PO2 and NADH fluorescence during synaptic stimulation and spreading depression reveals a transient dissociation between oxygen utilization and mitochondrial redox state in rat hippocampal slices. 
  • Synthesis and characterization of SERS gene probe for BRCA-1 (breast cancer). 
  • Targeted drug delivery by thermally responsive polymers. 
  • The alpha-helical domain of Galphat determines specific interaction with regulator of G protein signaling 9. 
  • The content of DNA and RNA in microparticles released by Jurkat and HL-60 cells undergoing in vitro apoptosis. 
  • The lightpipe luminoscope for monitoring occupational skin contamination. 
  • Total internal reflection fluorescence (TIRF) microscopy. I. Modelling cell contact region fluorescence. 
  • Tracking and quantification of single-walled carbon nanotubes in fish using near infrared fluorescence. 
  • Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers. 
  • Two-photon absorption properties of proquinoidal D-A-D and A-D-A quadrupolar chromophores. 
  • Urinary metabolities of harmine in the rat and their inhibition of monoamine oxidase. 
  • Use of fundus autofluorescence images to predict geographic atrophy progression. 
  • Variability of sun-induced chlorophyll fluorescence according to stand age-related processes in a managed loblolly pine forest. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Stinnett, Sandra Sue, Associate Professor of Biostatistics & Bioinformatics, Biostatistics & Bioinformatics
  • Turner, Dennis Alan, Professor of Neurosurgery, Biomedical Engineering