Genetic Complementation Test

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  Subject Areas on Research

  • A bifunctional dihydrofolate synthetase--folylpolyglutamate synthetase in Plasmodium falciparum identified by functional complementation in yeast and bacteria. 
  • A conserved alternative splice in the von Recklinghausen neurofibromatosis (NF1) gene produces two neurofibromin isoforms, both of which have GTPase-activating protein activity. 
  • A cysteine-rich nuclear protein activates yeast metallothionein gene transcription. 
  • A histidine protein kinase homologue required for regulation of bacterial cell division and differentiation. 
  • A mutation in Sec15l1 causes anemia in hemoglobin deficit (hbd) mice. 
  • A new dominant selectable marker for use in Cryptococcus neoformans. 
  • A new face of the Rhesus antigen. 
  • A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. 
  • A role for rat inositol polyphosphate kinases rIPK2 and rIPK1 in inositol pentakisphosphate and inositol hexakisphosphate production in rat-1 cells. 
  • A sensitized mutagenesis screen identifies Gli3 as a modifier of Sox10 neurocristopathy. 
  • A system for gene cloning and manipulation in the yeast Candida glabrata. 
  • A yeast expression system for human galactose-1-phosphate uridylyltransferase. 
  • ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene. 
  • Adenine auxotrophic mutants of Aspergillus oryzae: development of a novel transformation system with triple auxotrophic hosts. 
  • An essential role for an inositol polyphosphate multikinase, Ipk2, in mouse embryogenesis and second messenger production. 
  • Analysis of SCARECROW expression using a rapid system for assessing transgene expression in Arabidopsis roots. 
  • Analysis of meiotic recombination events near a recombination hotspot in the yeast Saccharomyces cerevisiae. 
  • Analysis of the neurofibromatosis type 1 (NF1) GAP-related domain by site-directed mutagenesis. 
  • Arabidopsis lox3 lox4 double mutants are male sterile and defective in global proliferative arrest. 
  • Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes. 
  • Bcl-2 accelerates multistep prostate carcinogenesis in vivo. 
  • CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19. 
  • CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1. 
  • CNS1 encodes an essential p60/Sti1 homolog in Saccharomyces cerevisiae that suppresses cyclophilin 40 mutations and interacts with Hsp90. 
  • Calcineurin governs thermotolerance and virulence of Cryptococcus gattii. 
  • Calcium binding is required for calmodulin function in Aspergillus nidulans. 
  • Carbonic anhydrase-8 regulates inflammatory pain by inhibiting the ITPR1-cytosolic free calcium pathway. 
  • Characterization of cis-regulatory regions responsible for developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana. 
  • Characterization of the Vibrio cholerae VolA surface-exposed lipoprotein lysophospholipase. 
  • Circulating PIG-A mutant T lymphocytes in healthy adults and patients with bone marrow failure syndromes. 
  • Cis-acting polymorphisms affect complex traits through modifications of microRNA regulation pathways. 
  • Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphate. 
  • Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae. 
  • Complementation of npf mutations in diploid amoebae of Physarum polycephalum: the basis for a general method of complementation analysis at the amoebal stage. 
  • Conservation and diversification of SCARECROW in maize. 
  • Conservation of a stress response: human heat shock transcription factors functionally substitute for yeast HSF. 
  • Contribution of horizontal gene transfer to the evolution of Saccharomyces cerevisiae. 
  • Cryptococcus neoformans Ilv2p confers resistance to sulfometuron methyl and is required for survival at 37 degrees C and in vivo. 
  • Cryptococcus neoformans histone acetyltransferase Gcn5 regulates fungal adaptation to the host. 
  • Derivation of a biologically contained replication system for human immunodeficiency virus type 1. 
  • Differential induction of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase genes in Arabidopsis thaliana by wounding and pathogenic attack. 
  • Dissection of the relative contribution of the Schizosaccharomyces pombe Ctr4 and Ctr5 proteins to the copper transport and cell surface delivery functions. 
  • Dominant selection system for use in Cryptococcus neoformans. 
  • Dual Molecular Effects of Dominant RORA Mutations Cause Two Variants of Syndromic Intellectual Disability with Either Autism or Cerebellar Ataxia. 
  • Elucidating the Candida albicans calcineurin signaling cascade controlling stress response and virulence. 
  • Expression and function of the uvsW gene of bacteriophage T4. 
  • Expression of functional bacterial undecaprenyl pyrophosphate synthase in the yeast rer2{Delta} mutant and CHO cells. 
  • Expression of the noncatalytic domain of the NIMA kinase causes a G2 arrest in Aspergillus nidulans. 
  • FAN1 mutations cause karyomegalic interstitial nephritis, linking chronic kidney failure to defective DNA damage repair. 
  • Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi. 
  • FimC is a periplasmic PapD-like chaperone that directs assembly of type 1 pili in bacteria. 
  • Fine structure mapping and phenotypic analysis of five temperature-sensitive mutations in the second largest subunit of vaccinia virus DNA-dependent RNA polymerase. 
  • Fragments of ATM which have dominant-negative or complementing activity. 
  • FtsZ from divergent foreign bacteria can function for cell division in Escherichia coli. 
  • Function of the hydrophilic carboxyl terminus of type II DNA topoisomerase from Drosophila melanogaster. II. In vivo studies. 
  • Functional analysis of the transcription control region located within the avian retroviral long terminal repeat. 
  • Functional complementation between FADD and RIP1 in embryos and lymphocytes. 
  • Functional conservation of the human homolog of the yeast pre-mRNA splicing factor Prp17p. 
  • Functional expression of the multidrug resistance-associated protein in the yeast Saccharomyces cerevisiae. 
  • Functional studies of the carboxy-terminal repeat domain of Drosophila RNA polymerase II in vivo. 
  • Gene therapy rescues cilia defects and restores olfactory function in a mammalian ciliopathy model. 
  • Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA. 
  • Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis. 
  • Genetic and biochemical characterization of mutants at an RNA polymerase II locus in D. melanogaster. 
  • Genetic changes associated with floral adaptation restrict future evolutionary potential. 
  • Genetic changes contributing to the parallel evolution of red floral pigmentation among Ipomoea species. 
  • Growth inhibition of a human lung adenocarcinoma cell line by genetic complementation with chromosome 11. 
  • Human immunodeficiency virus type 1 rev protein as a negative trans-regulator. 
  • Identification and analysis of a Saccharomyces cerevisiae copper homeostasis gene encoding a homeodomain protein. 
  • Identification and characterization of rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae: Evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles. 
  • Identification of a copper transporter family in Arabidopsis thaliana. 
  • Identification of a negative regulatory function for steroid receptors. 
  • Identification of the orthopoxvirus p4c gene, which encodes a structural protein that directs intracellular mature virus particles into A-type inclusions. 
  • Identification of the yeast cytidine deaminase CDD1 as an orphan C-->U RNA editase. 
  • Impact of ammonium permeases mepA, mepB, and mepC on nitrogen-regulated secondary metabolism in Fusarium fujikuroi. 
  • In vivo and in vitro examination of stability of primary hyperoxaluria-associated human alanine:glyoxylate aminotransferase. 
  • In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function. 
  • Indole-3-acetic acid biosynthesis is deficient in Gluconacetobacter diazotrophicus strains with mutations in cytochrome c biogenesis genes. 
  • Inositol diphosphate signaling regulates telomere length. 
  • Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69. 
  • Investigation of the basis of virulence in serotype A strains of Cryptococcus neoformans from apparently immunocompetent individuals. 
  • Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis. 
  • Involvement of the checkpoint protein Mec1p in silencing of gene expression at telomeres in Saccharomyces cerevisiae. 
  • Isolation and characterization of effector-loop mutants of CDC42 in yeast. 
  • Isolation of a metal-activated transcription factor gene from Candida glabrata by complementation in Saccharomyces cerevisiae. 
  • Isolation of a murine copper transporter gene, tissue specific expression and functional complementation of a yeast copper transport mutant. 
  • Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast. 
  • Laser-scanning velocimetry: a confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae. 
  • Life with too much polyprenol: polyprenol reductase deficiency. 
  • Loss of Function Mutations in NNT Are Associated With Left Ventricular Noncompaction. 
  • Loss of allergen 1 confers a hypervirulent phenotype that resembles mucoid switch variants of Cryptococcus neoformans. 
  • Major-gene resistance to the rust pathogen Coleosporium ipomoeae is common in natural populations of Ipomoea purpurea. 
  • Mammalian RAFT1 kinase domain provides rapamycin-sensitive TOR function in yeast. 
  • Mammalian metallothionein is functional in yeast. 
  • Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. 
  • Meiotic mutants that cause a polar decrease in recombination on the X chromosome in Caenorhabditis elegans. 
  • Methyl-directed repair of DNA base-pair mismatches in vitro. 
  • Modulation of human heat shock factor trimerization by the linker domain. 
  • Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function. 
  • Mutations in Saccharomyces cerevisiae which confer resistance to several amino acid analogs. 
  • Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase. 
  • Partial reconstitution of the RNAi response in human cells using Drosophila gene products. 
  • Rapamycin exerts antifungal activity in vitro and in vivo against Mucor circinelloides via FKBP12-dependent inhibition of Tor. 
  • Reconstitution of a thermophilic Cu+ importer in vitro reveals intrinsic high-affinity slow transport driving accumulation of an essential metal ion. 
  • Requirement for the vasa RNA helicase in gurken mRNA localization. 
  • Role of Cdc42p in pheromone-stimulated signal transduction in Saccharomyces cerevisiae. 
  • Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. 
  • SH1 domain autophosphorylation of P210 BCR/ABL is required for transformation but not growth factor independence. 
  • Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis. 
  • Site-specific mutations of FtsZ--effects on GTPase and in vitro assembly. 
  • Somatic mutation of hPMS2 as a possible cause of sporadic human colon cancer with microsatellite instability. 
  • Stat2 binding to the interferon-alpha receptor 2 subunit is not required for interferon-alpha signaling. 
  • Structural basis of lipid binding for the membrane-embedded tetraacyldisaccharide-1-phosphate 4'-kinase LpxK. 
  • Survey of Human Chromosome 21 Gene Expression Effects on Early Development in Danio rerio. 
  • Targeted resequencing and systematic in vivo functional testing identifies rare variants in MEIS1 as significant contributors to restless legs syndrome. 
  • Targets of immunophilin-immunosuppressant complexes are distinct highly conserved regions of calcineurin A. 
  • The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis. 
  • The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. 
  • The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. 
  • The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins. 
  • The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours. 
  • The beta sliding clamp binds to multiple sites within MutL and MutS. 
  • The epsilon subunit of DNA polymerase III Is involved in the nalidixic acid-induced SOS response in Escherichia coli. 
  • The genetic basis of a flower color polymorphism in the common morning glory (Ipomoea purpurea). 
  • The molecular basis of class II MHC allelic control of T cell responses. 
  • The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly. 
  • The regulation of DNA topoisomerase II by casein kinase II. 
  • The role of the supernumerary subunit of Rhodobacter sphaeroides cytochrome bc1 complex. 
  • Two new loci, PLEIADE and HYADE, implicate organ-specific regulation of cytokinesis in Arabidopsis. 
  • Two proteins encoded at the chlA locus constitute the converting factor of Escherichia coli chlA1. 
  • Using viral species specificity to define a critical protein/RNA interaction surface. 
  • Virulence of endemic nonpigmented northern Australian Staphylococcus aureus clone (clonal complex 75, S. argenteus) is not augmented by staphyloxanthin. 
  • crl mutants of Saccharomyces cerevisiae resemble both mutants affecting general control of amino acid biosynthesis and omnipotent translational suppressor mutants. 
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  Keywords of People

  • Hogan, Brigid L. M., George Barth Geller Professor, Cell Biology
  • Yan, Hai, Henry S. Friedman Professor of Neuro-Oncology in the School of Medicine, Pathology