Genetic Techniques

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  Subject Areas on Research

  • A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. 
  • A genetically modified allogeneic cellular vaccine generates MHC class I-restricted cytotoxic responses against tumor-associated antigens and protects against CNS tumors in vivo. 
  • A mosaic analysis system with Cre or Tomato expression in the mouse. 
  • A new assay to quantify in vivo repair of G:T mispairs by base excision repair. 
  • A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors. 
  • A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development. 
  • A regression-based association test for case-control studies that uses inferred ancestral haplotype similarity. 
  • A simple and improved correction for population stratification in case-control studies. 
  • A simple formula useful for positional cloning. 
  • An Improved Protocol for the Production of Lentiviral Vectors. 
  • An infant with hypoplastic left heart syndrome and spinal muscular atrophy. 
  • Association test for X-linked QTL in family-based designs. 
  • Biological clocks and the coordination theory of RNA operons and regulons. 
  • Bringing molecular tools into environmental resource management: untangling the molecules to policy pathway. 
  • CARHSP1 is required for effective tumor necrosis factor alpha mRNA stabilization and localizes to processing bodies and exosomes. 
  • Chromosome 3 analysis of uveal melanoma using fine-needle aspiration biopsy at the time of plaque radiotherapy in 140 consecutive cases: the Deborah Iverson, MD, Lectureship. 
  • Clinical and genetic investigation in autosomal dominant limb-girdle muscular dystrophy. 
  • Complementary techniques: RNA amplification for gene profiling analysis. 
  • Complex genetic disease: can genetic strategies in Alzheimer's disease and new genetic mechanisms be applied to epilepsy? 
  • Cryptococcus neoformans Mating and Genetic Crosses. 
  • Cryptococcus neoformans mitochondrial superoxide dismutase: an essential link between antioxidant function and high-temperature growth. 
  • Detection and reporting of homozygosity associated with consanguinity in the clinical laboratory. 
  • Direct allelic variation scanning of the yeast genome. 
  • Dissecting the architecture of a quantitative trait locus in yeast. 
  • Drosophila genome-wide RNAi screens: are they delivering the promise? 
  • Duchenne muscular dystrophy: high frequency of deletions. 
  • EMK: a novel program for family-based allelic and genotypic association tests on quantitative traits. 
  • Enabling functional genomics with genome engineering. 
  • Evolutionary genetics: gene replacement and the genetics of speciation. 
  • Experimental determination of rates of concerted evolution. 
  • Exploring molecular diversity with combinatorial shape libraries. 
  • Expression of Sry, the mouse sex determining gene. 
  • From genotype to phenotype: systems biology meets natural variation. 
  • Gene silencing using RNA interference in embryonic stem cells. 
  • Generation of Dhx9-deficient clones in T-cell development with a mitotic recombination technique. 
  • Genetic analysis for common complex disease. 
  • Genetic analysis of complex diseases. 
  • Genetic approaches to access cell types in mammalian nervous systems. 
  • Genetic approaches to neural circuits in the mouse. 
  • Genetic assimilation and accommodation: Models and mechanisms. 
  • Genetic studies in neural tube defects. NTD Collaborative Group. 
  • Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system. 
  • Genome technologies and personalized dental medicine. 
  • Genomic convergence to identify candidate genes for Parkinson disease: SAGE analysis of the substantia nigra. 
  • Genomic convergence: identifying candidate genes for Parkinson's disease by combining serial analysis of gene expression and genetic linkage. 
  • Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe. 
  • Hierarchy of RNA functional dynamics. 
  • Improvements to the differential display method for gene analysis. 
  • In vivo selection of tumor-targeting RNA motifs. 
  • Inbreeding depression in non-human primates: a historical review of methods used and empirical data. 
  • Inositol phosphate metabolomics: merging genetic perturbation with modernized radiolabeling methods. 
  • Knockdown of mouse VCAM-1 by vector-based siRNA. 
  • Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. 
  • Methods for precise sizing, automated binning of alleles, and reduction of error rates in large-scale genotyping using fluorescently labeled dinucleotide markers. FUSION (Finland-U.S. Investigation of NIDDM Genetics) Study Group. 
  • Microbial inactivation of Pseudomonas putida and Pichia pastoris using gene silencing. 
  • Mismatch repair proteins and mitotic genome stability. 
  • Molecular methods for epidemiological and diagnostic studies of fungal infections. 
  • Molecular typing of pathogenic fungi. 
  • Mps1 defines a proximal blastemal proliferative compartment essential for zebrafish fin regeneration. 
  • Pedigree selection and information content. 
  • Persistence and reversal of plasmid-mediated antibiotic resistance. 
  • PrimerZ: streamlined primer design for promoters, exons and human SNPs. 
  • Rapid assessment of repair of ultraviolet DNA damage with a modified host-cell reactivation assay using a luciferase reporter gene and correlation with polymorphisms of DNA repair genes in normal human lymphocytes. 
  • Rapid behavioral and genomic responses to social opportunity. 
  • Recombination in yeast and the recombinant DNA technology. 
  • Redirecting migration of T cells to chemokine secreted from tumors by genetic modification with CXCR2. 
  • Reiterative Recombination for the in vivo assembly of libraries of multigene pathways. 
  • Repair of mitomycin C cross-linked DNA in mammalian cells measured by a host cell reactivation assay. 
  • Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays. 
  • Sensitive digital quantification of DNA methylation in clinical samples. 
  • Small choroidal melanoma with chromosome 3 monosomy on fine-needle aspiration biopsy. 
  • Speciation genetics: evolving approaches. 
  • Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast. 
  • Strategy for investigating interactions between measured genes and measured environments. 
  • Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans. 
  • The Mutationathon highlights the importance of reaching standardization in estimates of pedigree-based germline mutation rates. 
  • The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs. 
  • The role of apolipoprotein E in Alzheimer's disease: pharmacogenomic target selection. 
  • The role of the mismatch repair machinery in regulating mitotic and meiotic recombination between diverged sequences in yeast. 
  • Transgenic animals in integrative biology: approaches and interpretations of outcome. 
  • Urine RNA Processing in a Clinical Setting: Comparison of 3 Protocols. 
  • Use of bacterial quorum-sensing components to regulate gene expression in plants. 
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  Keywords of People

  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Biomedical Engineering
  • Velkey, John Matthew, Assistant Professor of the Practice of Medical Education in the Department of Cell Biology, Cell Biology