Glycosylation

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  Subject Areas on Research

  • A Gastric Glycoform of MUC5AC Is a Biomarker of Mucinous Cysts of the Pancreas. 
  • A chemical glycoproteomics platform reveals O-GlcNAcylation of mitochondrial voltage-dependent anion channel 2. 
  • A common single nucleotide polymorphism A118G of the μ opioid receptor alters its N-glycosylation and protein stability. 
  • A conserved retromer-independent function for RAB-6.2 in C. elegans epidermis integrity. 
  • A designed glycoprotein analogue of Gc-MAF exhibits native-like phagocytic activity. 
  • A manganese porphyrin suppresses oxidative stress and extends the life span of streptozotocin-diabetic rats. 
  • A novel GALNT3 mutation in a pseudoautosomal dominant form of tumoral calcinosis: evidence that the disorder is autosomal recessive. 
  • A novel function of VCP (valosin-containing protein; p97) in the control of N-glycosylation of proteins in the endoplasmic reticulum. 
  • A novel inflammatory biomarker, GlycA, associates with disease activity in rheumatoid arthritis and cardio-metabolic risk in BMI-matched controls. 
  • A transcription factor links growth rate and metabolism in the hypersaline adapted archaeon Halobacterium salinarum. 
  • Active glycation in neurofibrillary pathology of Alzheimer disease: N(epsilon)-(carboxymethyl) lysine and hexitol-lysine. 
  • Advanced glycation end products contribute to amyloidosis in Alzheimer disease. 
  • Advances in enzymology and related areas of molecular biology. Preface. 
  • AglJ adds the first sugar of the N-linked pentasaccharide decorating the Haloferax volcanii S-layer glycoprotein. 
  • AglQ is a novel component of the Haloferax volcanii N-glycosylation pathway. 
  • AglR is required for addition of the final mannose residue of the N-linked glycan decorating the Haloferax volcanii S-layer glycoprotein. 
  • Altering the Immunogenicity of Hemagglutinin Immunogens by Hyperglycosylation and Disulfide Stabilization. 
  • Amplification and expression of the epidermal growth factor receptor gene in human glioma xenografts. 
  • An important role of l-fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. 
  • Antibody responses to the HIV-1 envelope high mannose patch. 
  • Antibody-independent, complement-mediated enhancement of HIV-1 infection by mannosidase I and II inhibitors. 
  • Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor. 
  • Assembling Glycan-Charged Dolichol Phosphates: Chemoenzymatic Synthesis of a Haloferax volcanii N-Glycosylation Pathway Intermediate. 
  • Association of single nucleotide polymorphisms in glycosylation genes with risk of epithelial ovarian cancer. 
  • Association of the Novel Inflammatory Marker GlycA and Incident Heart Failure and Its Subtypes of Preserved and Reduced Ejection Fraction: The Multi-Ethnic Study of Atherosclerosis. 
  • Behçet's disease patients present high levels of deglycosylated anti-lipoteichoic acid IgG and high IL-8 production after lipoteichoic acid stimulation. 
  • Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits. 
  • Broad Analysis of Vicinal Disulfides: Occurrences, Conformations with Cis or with Trans Peptides, and Functional Roles Including Sugar Binding. 
  • Broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza virus hemagglutinin. 
  • Cell adhesion molecule L1 in folded (horseshoe) and extended conformations. 
  • Cells with pathogenic biallelic mutations in the human MUTYH gene are defective in DNA damage binding and repair. 
  • Changes in oligosaccharide chains of autoantibodies to GRP78 expressed during progression of malignant melanoma stimulate melanoma cell growth and survival. 
  • Characterization of glycosylation profiles of HIV-1 transmitted/founder envelopes by mass spectrometry. 
  • Characterization of host-cell line specific glycosylation profiles of early transmitted/founder HIV-1 gp120 envelope proteins. 
  • Characterization of the PMT gene family in Cryptococcus neoformans. 
  • Chemoenzymatic Assembly of Bacterial Glycoconjugates for Site-Specific Orthogonal Labeling. 
  • Chondroitin 4-sulfate covalently cross-links the chains of the human blood protein pre-alpha-inhibitor. 
  • Cloning, expression, and cellular localization of a human prenylcysteine lyase. 
  • Coming together at the hinges: Therapeutic prospects of IgG3. 
  • Comparative Analysis of the Glycosylation Profiles of Membrane-Anchored HIV-1 Envelope Glycoprotein Trimers and Soluble gp140. 
  • Comparison of HPLC/ESI-FTICR MS versus MALDI-TOF/TOF MS for glycopeptide analysis of a highly glycosylated HIV envelope glycoprotein. 
  • Comparison of the molecular properties of retinitis pigmentosa P23H and N15S amino acid replacements in rhodopsin. 
  • Comparison of viral Env proteins from acute and chronic infections with subtype C human immunodeficiency virus type 1 identifies differences in glycosylation and CCR5 utilization and suggests a new strategy for immunogen design. 
  • Complement-mediated binding of naturally glycosylated and glycosylation-modified human immunodeficiency virus type 1 to human CR2 (CD21). 
  • Core N-Glycan Structures Are Critical for the Pathogenicity of Cryptococcus neoformans by Modulating Host Cell Death. 
  • Design, expression, and immunogenicity of a soluble HIV trimeric envelope fragment adopting a prefusion gp41 configuration. 
  • Determinants of Tenascin-C and HIV-1 envelope binding and neutralization. 
  • Different routes to the same ending: comparing the N-glycosylation processes of Haloferax volcanii and Haloarcula marismortui, two halophilic archaea from the Dead Sea. 
  • Difficult-to-neutralize global HIV-1 isolates are neutralized by antibodies targeting open envelope conformations. 
  • Disrupted N-linked glycosylation as a disease mechanism in deficiency of ADA2. 
  • Distinct glycan-charged phosphodolichol carriers are required for the assembly of the pentasaccharide N-linked to the Haloferax volcanii S-layer glycoprotein. 
  • Distinct patterns of glycosylation of colligin, a collagen-binding glycoprotein, and SPARC (osteonectin), a secreted Ca2+-binding glycoprotein. Evidence for the localisation of colligin in the endoplasmic reticulum. 
  • Diversity in prokaryotic glycosylation: an archaeal-derived N-linked glycan contains legionaminic acid. 
  • Dynamic Glycosylation Governs the Vertebrate COPII Protein Trafficking Pathway. 
  • Early AGEing and Alzheimer's. 
  • Eliminating antibody polyreactivity through addition of N-linked glycosylation. 
  • Envelope deglycosylation enhances antigenicity of HIV-1 gp41 epitopes for both broad neutralizing antibodies and their unmutated ancestor antibodies. 
  • Erythrocyte Webb-type glycophorin C variant lacks N-glycosylation due to an asparagine to serine substitution. 
  • Erythropoietin structure-function relationships. Mutant proteins that test a model of tertiary structure. 
  • Evidence against posttranslational glycosylation of rat glucocorticoid receptors. 
  • Evidence for a novel O-linked sialylated trisaccharide on Ser-248 of human plasminogen 2. 
  • Evidence for nutrient-dependent regulation of the COPII coat by O-GlcNAcylation. 
  • Evidence that mannosyl residues are involved in human immunodeficiency virus type 1 (HIV-1) pathogenesis. 
  • Ex Vivo Expansion or Manipulation of Stem Cells to Improve Outcome of Umbilical Cord Blood Transplantation. 
  • Exploring Glycan Markers for Immunotyping and Precision-targeting of Breast Circulating Tumor Cells. 
  • Expression analysis of endoglin missense and truncation mutations: insights into protein structure and disease mechanisms. 
  • Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies. 
  • Fc Characteristics Mediate Selective Placental Transfer of IgG in HIV-Infected Women. 
  • Functional analysis of the lipoglycodepsipeptide antibiotic ramoplanin. 
  • Generation and Characterization of a Bivalent HIV-1 Subtype C gp120 Protein Boost for Proof-of-Concept HIV Vaccine Efficacy Trials in Southern Africa. 
  • Generation and characterization of a bivalent protein boost for future clinical trials: HIV-1 subtypes CR01_AE and B gp120 antigens with a potent adjuvant. 
  • Gigaxonin glycosylation regulates intermediate filament turnover and may impact giant axonal neuropathy etiology or treatment 
  • GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis for inhibition of N-glycosylation. 
  • Glutamine enhances heat shock protein 70 expression via increased hexosamine biosynthetic pathway activity. 
  • Glutamine induces heat shock protein expression via O-glycosylation and phosphorylation of HSF-1 and Sp1. 
  • Glycated AAV vectors: chemical redirection of viral tissue tropism. 
  • Glyco-engineering in Archaea: differential N-glycosylation of the S-layer glycoprotein in a transformed Haloferax volcanii strain. 
  • Glycoengineering HIV-1 Env creates 'supercharged' and 'hybrid' glycans to increase neutralizing antibody potency, breadth and saturation. 
  • Glycoprotein B of human herpesvirus 8 is a component of the virion in a cleaved form composed of amino- and carboxyl-terminal fragments. 
  • Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity. 
  • Glycosylation Benchmark Profile for HIV-1 Envelope Glycoprotein Production Based on Eleven Env Trimers. 
  • Glycosylation influences intracellular transit time and secretion rate of human prorenin in transfected cells. 
  • Glycosylation of KEAP1 links nutrient sensing to redox stress signaling. 
  • Glycosylation of proteinase 3 (PR3) is not required for its reactivity with antineutrophil cytoplasmic antibodies (ANCA) in Wegener's granulomatosis. 
  • Glycosylation site-specific analysis of HIV envelope proteins (JR-FL and CON-S) reveals major differences in glycosylation site occupancy, glycoform profiles, and antigenic epitopes' accessibility. 
  • Glycosylation site-specific analysis of clade C HIV-1 envelope proteins. 
  • HIV-1 envelope glycan modifications that permit neutralization by germline-reverted VRC01-class broadly neutralizing antibodies. 
  • HIV-1 intrapatient sequence diversity in the immunogenic V3 region. 
  • Heterogeneity in the B1 (CD20) cell surface molecule expressed by human B-lymphocytes. 
  • Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function. 
  • Hyperglycosylated human chorionic gonadotropin does not increase progesterone production by luteinized granulosa cells. 
  • Identification and replication of novel genetic variants of ABO gene to reduce the incidence of diseases and promote longevity by modulating lipid homeostasis. 
  • Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier. 
  • Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals. 
  • Increasing O-GlcNAcylation is neuroprotective in young and aged brains after ischemic stroke. 
  • Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope. 
  • Involvement of histo-blood-group antigens in the susceptibility of colon carcinoma cells to natural killer-mediated cytotoxicity. 
  • Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis. 
  • Is amyloid beta-protein glycated in Alzheimer's disease? 
  • Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields. 
  • L-selectin binds to P-selectin glycoprotein ligand-1 on leukocytes: interactions between the lectin, epidermal growth factor, and consensus repeat domains of the selectins determine ligand binding specificity. 
  • Life with too much polyprenol: polyprenol reductase deficiency. 
  • Lipid modification gives rise to two distinct Haloferax volcanii S-layer glycoprotein populations. 
  • Lipid sugar carriers at the extremes: The phosphodolichols Archaea use in N-glycosylation. 
  • Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells. 
  • Membrane insertion, glycosylation, and oligomerization of inositol trisphosphate receptors in a cell-free translation system. 
  • Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway. 
  • Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype. 
  • Motifs within the CLN3 protein: modulation of cell growth rates and apoptosis. 
  • Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation. 
  • Mutational analysis of conserved N-linked glycosylation sites of human immunodeficiency virus type 1 gp41. 
  • Myelodysplasia and deficiency of uridine diphosphate-galactose 4-epimerase. 
  • N-Linked Glycans Are Assembled on Highly Reduced Dolichol Phosphate Carriers in the Hyperthermophilic Archaea Pyrococcus furiosus. 
  • N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier. 
  • N-linked glycosylation of protease-activated receptor-1 at extracellular loop 2 regulates G-protein signaling bias. 
  • Neonatal plasminogen displays altered cell surface binding and activation kinetics. Correlation with increased glycosylation of the protein. 
  • NgBR is essential for endothelial cell glycosylation and vascular development. 
  • Nonrandom distribution of gp120 N-linked glycosylation sites important for infectivity of human immunodeficiency virus type 1. 
  • Novel nucleocytoplasmic protein O-fucosylation by SPINDLY regulates diverse developmental processes in plants. 
  • Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock. 
  • O-glycosylation as a sorting determinant for cell surface delivery in yeast. 
  • Parallel Glyco-SPOT Synthesis of Glycopeptide Libraries. 
  • Partial glycosylation at asparagine-2181 of the second C-type domain of human factor V modulates assembly of the prothrombinase complex. 
  • Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM. 
  • Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes. 
  • Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90. 
  • Potential pathogenicity of deglycosylated IgG cross reactive with streptokinase and fibronectin in the serum of patients with rheumatoid arthritis. 
  • Prediction of VRC01 neutralization sensitivity by HIV-1 gp160 sequence features. 
  • Protein glycosylation as an adaptive response in Archaea: growth at different salt concentrations leads to alterations in Haloferax volcanii S-layer glycoprotein N-glycosylation. 
  • Purification and partial molecular characterization of GRP94, an ER resident chaperone. 
  • Purification, characterization, and immunogenicity of a soluble trimeric envelope protein containing a partial deletion of the V2 loop derived from SF162, an R5-tropic human immunodeficiency virus type 1 isolate. 
  • Quantification of the Impact of the HIV-1-Glycan Shield on Antibody Elicitation. 
  • Quaternary epitope specificities of anti-HIV-1 neutralizing antibodies generated in rhesus macaques infected by the simian/human immunodeficiency virus SHIVSF162P4. 
  • Recurrent signature patterns in HIV-1 B clade envelope glycoproteins associated with either early or chronic infections. 
  • Reducing V3 Antigenicity and Immunogenicity on Soluble, Native-Like HIV-1 Env SOSIP Trimers. 
  • Revisiting N-glycosylation in Halobacterium salinarum: Characterizing a dolichol phosphate- and glycoprotein-bound tetrasaccharide. 
  • RoBo-1, a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and growth plate cartilage. 
  • Role of protein N-glycosylation in pathogenesis of human immunodeficiency virus type 1. 
  • Role of sodium channel deglycosylation in the genesis of cardiac arrhythmias in heart failure. 
  • SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder. 
  • Safety and immunogenicity of a fully glycosylated recombinant gp160 human immunodeficiency virus type 1 vaccine in subjects at low risk of infection. National Institute of Allergy and Infectious Diseases AIDS Vaccine Evaluation Group Network. 
  • Salt stress increases abundance and glycosylation of CFTR localized at apical surfaces of salt gland secretory cells. 
  • Self-organization of apical membrane protein sorting in epithelial cells. 
  • Sialic acid content of plasminogen 2 glycoforms as a regulator of fibrinolytic activity. Isolation, carbohydrate analysis, and kinetic characterization of six glycoforms of plasminogen. 
  • Site-specific glycosylation regulates the form and function of the intermediate filament cytoskeleton. 
  • Specific glycoforms of MUC5AC and endorepellin accurately distinguish mucinous from nonmucinous pancreatic cysts. 
  • Structural Basis of WLS/Evi-Mediated Wnt Transport and Secretion. 
  • Structural basis for diverse N-glycan recognition by HIV-1-neutralizing V1-V2-directed antibody PG16. 
  • Structure and immune recognition of trimeric pre-fusion HIV-1 Env. 
  • Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9. 
  • Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein. 
  • Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans. 
  • Synthesis and bioactivity of carbohydrate derivatives of indigo, its isomers and heteroanalogues. 
  • Synthesis and evaluation of glycosylated octreotate analogues labeled with radioiodine and 211At via a tin precursor. 
  • Synthetic HIV V3 Glycopeptide Immunogen Carrying a N334 N-Glycan Induces Glycan-Dependent Antibodies with Promiscuous Site Recognition. 
  • TNFalpha and IL-4 regulation of hyaluronan binding to monocyte CD44 involves posttranslational modification of CD44. 
  • Targeting the hexosamine biosynthetic pathway and O-linked N-acetylglucosamine cycling for therapeutic and imaging capabilities in diffuse large B-cell lymphoma. 
  • Terminal N-linked galactose is the primary receptor for adeno-associated virus 9. 
  • The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template. 
  • The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization. 
  • The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans. 
  • The SARS-CoV-2 Spike variant D614G favors an open conformational state. 
  • The acceptor substrate specificity of porcine submaxillary UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is dependent on the amino acid sequences adjacent to serine and threonine residues. 
  • The contribution of Fc effector mechanisms in the efficacy of anti-CD154 immunotherapy depends on the nature of the immune challenge. 
  • The loss of a polymorphic glycosylation site caused by Thr-927-->Ile is linked to a second polymorphic Val-816-->Ile substitution in lysosomal alpha-glucosidase of American blacks. 
  • The structure of irisin reveals a novel intersubunit β-sheet fibronectin type III (FNIII) dimer: implications for receptor activation. 
  • Tissue factor is the receptor for plasminogen type 1 on 1-LN human prostate cancer cells. 
  • Topology of mammalian isoprenylcysteine carboxyl methyltransferase determined in live cells with a fluorescent probe. 
  • Total synthesis of cyanolide A and confirmation of its absolute configuration. 
  • Two distinct N-glycosylation pathways process the Haloferax volcanii S-layer glycoprotein upon changes in environmental salinity. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor of Cell Biology, Cell Biology
  • Guan, Ziqiang, Research Professor in Biochemistry, Biochemistry