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Subject Areas on Research
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A Unified Model for Treadmilling and Nucleation of Single-Stranded FtsZ Protofilaments.
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A rapid fluorescence assay for FtsZ assembly indicates cooperative assembly with a dimer nucleus.
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A reconstitution assay for the guanine nucleotide regulatory protein of the adenylate cyclase system using turkey erythrocyte membranes as the acceptor preparation.
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A region of adenylyl cyclase 2 critical for regulation by G protein beta gamma subunits.
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Abl family kinases modulate T cell-mediated inflammation and chemokine-induced migration through the adaptor HEF1 and the GTPase Rap1.
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Activation of RalA is critical for Ras-induced tumorigenesis of human cells.
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Activation of adenylate cyclase by phosphoramidate and phosphonate analogs of GTP: possible role of covalent enzyme-substrate intermediates in the mechanism of hormonal activation.
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Adrenergic desensitization in left ventricle from streptozotocin diabetic swine.
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Adrenergic receptors: molecular mechanisms of clinically relevant regulation.
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Affinity of (alpha-P-borano)-NTP analogs to rabbit muscle pyruvate kinase.
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Agonist-promoted coupling of the beta-adrenergic receptor with the guanine nucleotide regulatory protein of the adenylate cyclase system.
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Agonist-specific effects of guanine nucleotides on alpha-adrenergic receptors in human platelets.
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Altered activity of the nucleotide regulatory site in the parathyroid hormone-sensitive adenylate cyclase from the renal cortex of a patient with pseudohypoparathyroidism.
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Analysis of the neurofibromatosis type 1 (NF1) GAP-related domain by site-directed mutagenesis.
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Antitumor agents. 139. Synthesis and biological evaluation of thiocolchicine analogs 5,6-dihydro-6(S)-(acyloxy)- and 5,6-dihydro-6(S)-[(aroyloxy)methyl]-1,2,3-trimethoxy-9-(methylthio)-8H- cyclohepta[a]naphthalen-8-ones as novel cytotoxic and antimitotic agents.
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Apparent cooperative assembly of the bacterial cell division protein FtsZ demonstrated by isothermal titration calorimetry.
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Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins.
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Assembly dynamics of Mycobacterium tuberculosis FtsZ.
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Assembly of pure tubulin in the absence of free GTP: effect of magnesium, glycerol, ATP, and the nonhydrolyzable GTP analogues.
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Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p.
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Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors.
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Bacterial cell division protein FtsZ assembles into protofilament sheets and minirings, structural homologs of tubulin polymers.
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Beta-adrenergic inhibition of cardiac sodium channels by dual G-protein pathways.
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Beta-adrenergic receptor overexpression in the fetal rat: distribution, receptor subtypes, and coupling to adenylate cyclase activity via G-proteins.
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Beta-adrenoceptor control of cardiac adenylyl cyclase during development: agonist pretreatment in the neonate uniquely causes heterologous sensitization, not desensitization.
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Binding of rat alpha 1-inhibitor-3-methylamine to the alpha 2-macroglobulin signaling receptor induces second messengers.
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Biochemical and immunological abnormalities in purine nucleoside phosphorylase deficient mice.
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BtubA-BtubB heterodimer is an essential intermediate in protofilament assembly.
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C-Terminal glycine-gated radical initiation by GTP 3',8-cyclase in the molybdenum cofactor biosynthesis.
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Catecholamine stimulated myocardial adenylate cyclase: effects of nucleotides.
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Cdc42p, GTP hydrolysis, and the cell's sense of direction.
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Cellular mechanisms for developmental toxicity of chlorpyrifos: targeting the adenylyl cyclase signaling cascade.
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Characteristics of 5'-guanylyl imidodiphosphate-activated adenylate cyclase.
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Characteristics of GTP-mediated microsomal Ca2+ release.
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Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4.
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Characterization of two different five-coordinate soluble guanylate cyclase ferrous-nitrosyl complexes.
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Clinical physiology of adrenergic receptor regulation.
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Coat proteins and selective protein packaging into transport vesicles.
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Commentary. Molecular pharmacology of beta-adrenergic receptors--a status report.
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Compartmentalization and regulation of GTP in control of cellular phenotypes.
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Complete hematologic and cytogenetic response to 2-amino-9-beta-D-arabinosyl-6-methoxy-9H-guanine in a patient with chronic myelogenous leukemia in T-cell blastic phase: a case report and review of the literature.
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Compound GW506U78 in refractory hematologic malignancies: relationship between cellular pharmacokinetics and clinical response.
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Coordinate regulation of forskolin-induced cellular proliferation in macrophages by protein kinase A/cAMP-response element-binding protein (CREB) and Epac1-Rap1 signaling: effects of silencing CREB gene expression on Akt activation.
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Correlation of beta-adrenergic receptor-stimulated [3H]GDP release and adenylate cyclase activation. Differences between frog and turkey erythrocyte membranes.
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Cyclocreatine (1-carboxymethyl-2-iminoimidazolidine) inhibits the replication of human herpes viruses.
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DNA repair protein O6-alkylguanine-DNA alkyltransferase is phosphorylated by two distinct and novel protein kinases in human brain tumour cells.
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Definition of a consensus transportin-specific nucleocytoplasmic transport signal.
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Dependence of leucine-rich repeat kinase 2 (LRRK2) kinase activity on dimerization.
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Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides.
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Differential effects of GTP on the coupling of beta-adrenergic receptors to adenylate cyclase from frog and turkey erythrocytes. Application of new methods for the analysis of receptor-effector coupling.
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Dilution-induced disassembly of microtubules: relation to dynamic instability and the GTP cap.
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Direct evidence that Gi-coupled receptor stimulation of mitogen-activated protein kinase is mediated by G beta gamma activation of p21ras.
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Disrupting the ciliary gradient of active Arl3 affects rod photoreceptor nuclear migration.
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Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
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Effects of magnesium on the dynamic instability of individual microtubules.
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Endogenous proteinases modulate the function of the beta-adrenergic receptor-adenylate cyclase system.
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Endothelin activates voltage-dependent Ca2+ current by a G protein-dependent mechanism in rabbit cardiac myocytes.
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Enhanced adenylate cyclase activity of turkey erythrocytes following treatment with beta-adrenergic receptor antagonists.
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Enzymology of GTPase acceleration in phototransduction.
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Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression.
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Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion.
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Expression of cDNAs for G proteins in Escherichia coli. Two forms of Gs alpha stimulate adenylate cyclase.
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FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis.
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FtsZ from Escherichia coli, Azotobacter vinelandii, and Thermotoga maritima--quantitation, GTP hydrolysis, and assembly.
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Functional differences in the beta gamma complexes of transducin and the inhibitory guanine nucleotide regulatory protein.
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G protein involvement in receptor-effector coupling.
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G proteins in medicine.
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GTP hydrolysis during microtubule assembly.
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GTP-mediated Ca2+ release in rough endoplasmic reticulum. Correlation with a GTP-sensitive increase in membrane permeability.
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Glucocorticoid-targeting of the adenylyl cyclase signaling pathway in the cerebellum of young vs. aged rats.
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Glucocorticoids enhance intracellular signaling via adenylate cyclase at three distinct loci in the fetus: a mechanism for heterologous teratogenic sensitization?
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Glucocorticoids regulate the development of intracellular signaling: enhanced forebrain adenylate cyclase catalytic subunit activity after fetal dexamethasone exposure.
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Guanine arabinoside as a bone marrow-purging agent.
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Guanine nucleotides modulate the binding affinity of the oligopeptide chemoattractant receptor on human polymorphonuclear leukocytes.
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Guanosine triphosphate binding sites in solubilized myocardium. Relation to adenylate cyclase activity.
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Guanosine triphosphate links MYC-dependent metabolic and ribosome programs in small-cell lung cancer.
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Guanosinetriphosphatase activity of tubulin associated with microtubule assembly.
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Gz, a guanine nucleotide-binding protein with unique biochemical properties.
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Heterogeneity of radioligand binding to alpha-adrenergic receptors. Analysis of guanine nucleotide regulation of agonist binding in relation to receptor subtypes.
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High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP.
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Homologous desensitization of the beta-adrenergic receptor. Functional integrity of the desensitized receptor from mammalian lung.
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Hormonal inhibition of adenylate cyclase. alpha 2 Adrenergic receptors promote release of [3H]guanylylimidodiphosphate from platelet membranes.
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Hormone-sensitive adenylate cyclase. Delineation of a trypsin-sensitive site in the pathway of receptor-mediated inhibition.
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Human substance P receptor expressed in Chinese hamster ovary cells directly activates G(alpha q/11), G(alpha s), G(alpha o).
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Identification and characterization of a leucine-rich repeat kinase 2 (LRRK2) consensus phosphorylation motif.
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Identification and initial characterization of translational initiation factor 2 from bovine mitochondria.
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Identification of chemical inhibitors to human tissue transglutaminase by screening existing drug libraries.
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Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets.
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In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB.
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In vitro assembly studies of FtsZ/tubulin-like proteins (TubZ) from Bacillus plasmids: evidence for a capping mechanism.
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Incorporation of ribonucleoside 5'-(alpha-P-borano)triphosphates into a 20-mer RNA by T7 RNA polymerase.
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Incorporation of tyrosine and glutamine residues into the soluble guanylate cyclase heme distal pocket alters NO and O2 binding.
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Inhibition of cardiac Na+ currents by isoproterenol.
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Inside-out Z rings--constriction with and without GTP hydrolysis.
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Interaction sites of the COOH-terminal region of the gamma subunit of cGMP phosphodiesterase with the GTP-bound alpha subunit of transducin.
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Intrinsic GTP hydrolysis is observed for a switch 1 variant of Cdc42 in the presence of a specific GTPase inhibitor.
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Irreversible activation of adenylate cyclase of toad erythrocyte plasma membrane by 5'-guanylylimidodiphosphate.
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Irreversible stimulation of adenylate cyclase activity of fat cell membranes of phosphoramidate and phosphonate analogs of GTP.
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Isobutylmethylxanthine stimulates adenylate cyclase by blocking the inhibitory regulatory protein, Gi.
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Isolation of GTP-binding proteins from myeloid HL-60 cells. Identification of two pertussis toxin substrates.
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Kinetics of irreversible activation of adenylate cyclase of fat cell membranes by phosphonium and phosphoramidate analogs of gtp1.
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LRRK2 autophosphorylation enhances its GTPase activity.
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LRRK2 phosphorylates membrane-bound Rabs and is activated by GTP-bound Rab7L1 to promote recruitment to the trans-Golgi network.
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Ligation of the alpha2M signalling receptor elevates the levels of p21Ras-GTP in macrophages.
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Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase.
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Mechanism of Reduction of an Aminyl Radical Intermediate in the Radical SAM GTP 3',8-Cyclase MoaA.
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Mechanism of action of Vibrio cholerae enterotoxin. Effects on adenylate cyclase of toad and rat erythrocyte plasma membranes.
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Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin. Relations to the mode of activation by hormones.
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Mechanism of guanine nucleotide regulatory protein-mediated inhibition of adenylate cyclase. Studies with isolated subunits of transducin in a reconstituted system.
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Mechanisms of hormone receptor-effector coupling: the beta-adrenergic receptor and adenylate cyclase.
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Microinjection of a 19-kDa guanine nucleotide-binding protein inhibits maturation of Xenopus oocytes.
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Microtubule dynamic instability and GTP hydrolysis.
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Modeling the physics of FtsZ assembly and force generation.
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Molecular determinants of selectivity in 5-hydroxytryptamine1B receptor-G protein interactions.
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Molecular pharmacology of adenylate cyclase-coupled alpha- and beta-adrenergic receptors.
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Multiple cyclic AMP receptors are linked to adenylyl cyclase in Dictyostelium.
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Multiple effects of guanine nucleotides on human platelet adenylated cyclase.
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Mutation-specific RAS oncogenicity explains NRAS codon 61 selection in melanoma.
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Myocardial guanylate cyclase: properties of the enzyme and effects of cholinergic agonists in vitro.
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Nascent secretory chain binding and translocation are distinct processes: differentiation by chemical alkylation.
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Novel pathogenic LRRK2 p.Asn1437His substitution in familial Parkinson's disease.
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Nucleotide regulation of soluble guanylate cyclase substrate specificity.
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Nucleotide regulatory protein-mediated activation of phospholipase C in human polymorphonuclear leukocytes is disrupted by phorbol esters.
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Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region.
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On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.
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One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity.
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Ontogeny of regulatory mechanisms for beta-adrenoceptor control of rat cardiac adenylyl cyclase: targeting of G-proteins and the cyclase catalytic subunit.
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Pharmacologic purging of malignant T cells from human bone marrow using 9-beta-D-arabinofuranosylguanine.
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Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.
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Phosphorylation of the beta-adrenergic receptor in intact cells: relationship to heterologous and homologous mechanisms of adenylate cyclase desensitization.
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Polycation-induced assembly of purified tubulin.
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Polyethylene glycol-stimulated microsomal GTP hydrolysis. Relationship to GTP-mediated Ca2+ release.
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Polymerization of Ftsz, a bacterial homolog of tubulin. is assembly cooperative?
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Pregnancy and estradiol modulate myometrial G-protein pathways in the guinea pig.
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Purification and assembly of FtsZ.
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Purine nucleoside phosphorylase deficiency.
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RGS10 is a selective activator of G alpha i GTPase activity.
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RGSZ1, a Gz-selective regulator of G protein signaling whose action is sensitive to the phosphorylation state of Gzalpha.
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Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells.
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Radical Breakthroughs in Natural Product and Cofactor Biosynthesis.
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Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer.
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Rapid in vitro assembly of Caulobacter crescentus FtsZ protein at pH 6.5 and 7.2.
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Reconstitution of a hormone-sensitive adenylate cyclase system. The pure beta-adrenergic receptor and guanine nucleotide regulatory protein confer hormone responsiveness on the resolved catalytic unit.
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Reconstitution of nuclear protein export in isolated nuclear envelopes.
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Regulation of beta-adrenergic receptors by guanyl-5'-yl imidodiphosphate and other purine nucleotides.
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Regulation of deactivation of photoreceptor G protein by its target enzyme and cGMP.
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Regulation of hormone receptors and adenylyl cyclases by guanine nucleotide binding N proteins.
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Regulation of local GTP availability controls RAC1 activity and cell invasion.
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Regulation of mitogen-activated protein kinase pathways by catecholamine receptors.
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Regulation of transducin GTPase activity in bovine rod outer segments.
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Rodbell and Gilman win 1994 Nobel Prize for Physiology and Medicine.
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Role for the target enzyme in deactivation of photoreceptor G protein in vivo.
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Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization.
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SU5416 delays wound healing through inhibition of TGF-beta 1 activation.
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Scaffold-mediated symmetry breaking by Cdc42p.
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Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.
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Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p.
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Site-specific mutations of FtsZ--effects on GTPase and in vitro assembly.
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Sites of circularization of the Tetrahymena rRNA IVS are determined by sequence and influenced by position and secondary structure.
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Specificity of the functional interactions of the beta-adrenergic receptor and rhodopsin with guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles.
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Stimulation of catecholamine-sensitive adenylate cyclase by 5'-guanylyl-imidodiphosphate.
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Straight and curved conformations of FtsZ are regulated by GTP hydrolysis.
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Structural and functional studies of cyclic GMP phosphodiesterase.
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Structural and signaling requirements for BCR-ABL-mediated transformation and inhibition of apoptosis.
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Subcellular localization and functional analysis of the Arabidopsis GTPase RabE.
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Subtype-specific binding of azidoanilido-GTP by purified G protein alpha subunits.
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Synergistic inhibition of human leukemia cell growth by deoxyguanosine and 1-beta-D-arabinofuranosylcytosine.
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Synthesis and evaluation of a phosphonate analogue of the soluble guanylate cyclase activator YC-1.
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The 2.0 A crystal structure of a heterotrimeric G protein.
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The Chloroplast Tubulin Homologs FtsZA and FtsZB from the Red Alga Galdieria sulphuraria Co-assemble into Dynamic Filaments.
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The E2-like conjugation enzyme Atg3 promotes binding of IRG and Gbp proteins to Chlamydia- and Toxoplasma-containing vacuoles and host resistance.
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The Lipid Kinase PI5P4Kβ Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis.
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The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro.
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The formylpeptide chemoattractant receptor copurifies with a GTP-binding protein containing a distinct 40-kDa pertussis toxin substrate.
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The inducibly expressed GTPase localizes to the endoplasmic reticulum, independently of GTP binding.
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The kinetics of microtubule assembly. Evidence for a two-stage nucleation mechanism.
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The new cardiotonic agent sulmazole is an A1 adenosine receptor antagonist and functionally blocks the inhibitory regulator, Gi.
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The straight and curved conformation of FtsZ protofilaments-evidence for rapid exchange of GTP into the curved protofilament.
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The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase.
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The structure and assembly of microtubules.
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Ubiquitination of K-Ras enhances activation and facilitates binding to select downstream effectors.
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Unique uncoupling of the frog erythrocyte adenylate cyclase system by manganese. Loss of hormone and guanine nucleotide-sensitive enzyme activities without loss of nucleotide-sensitive, high affinity agonist binding.
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Using Cryo-EM to Map Small Ligands on Dynamic Metabolic Enzymes: Studies with Glutamate Dehydrogenase.
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Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends.
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ZipA and FtsA* stabilize FtsZ-GDP miniring structures.
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beta-Arrestin-mediated ADP-ribosylation factor 6 activation and beta 2-adrenergic receptor endocytosis.
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cGMP suppresses GTPase activity of a portion of transducin equimolar to phosphodiesterase in frog rod outer segments. Light-induced cGMP decreases as a putative feedback mechanism of the photoresponse.
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Keywords of People
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Bennett, Vann,
George Barth Geller Distinguished Professor of Molecular Biology,
Duke Cancer Institute
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Erickson, Harold Paul,
Professor Emeritus of Cell Biology,
Cell Biology
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Pitt, Geoffrey Stuart,
Adjunct Professor in the Department of Medicine,
Medicine, Cardiology
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Taylor, Gregory Alan,
Professor in Medicine,
Immunology