Guanosine Triphosphate

• 

  Subject Areas on Research

  • A functional role for GTP-binding proteins in synaptic vesicle cycling. 
  • A rapid fluorescence assay for FtsZ assembly indicates cooperative assembly with a dimer nucleus. 
  • A reconstitution assay for the guanine nucleotide regulatory protein of the adenylate cyclase system using turkey erythrocyte membranes as the acceptor preparation. 
  • A region of adenylyl cyclase 2 critical for regulation by G protein beta gamma subunits. 
  • ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation. 
  • ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat. 
  • Abl family kinases modulate T cell-mediated inflammation and chemokine-induced migration through the adaptor HEF1 and the GTPase Rap1. 
  • Activation of RalA is critical for Ras-induced tumorigenesis of human cells. 
  • Activation of adenylate cyclase by phosphoramidate and phosphonate analogs of GTP: possible role of covalent enzyme-substrate intermediates in the mechanism of hormonal activation. 
  • Adrenergic desensitization in left ventricle from streptozotocin diabetic swine. 
  • Adrenergic receptors: molecular mechanisms of clinically relevant regulation. 
  • Affinity of (alpha-P-borano)-NTP analogs to rabbit muscle pyruvate kinase. 
  • Agonist binding promotes a guanine nucleotide reversible increase in the apparent size of the bovine anterior pituitary dopamine receptors. 
  • Agonist-promoted coupling of the beta-adrenergic receptor with the guanine nucleotide regulatory protein of the adenylate cyclase system. 
  • Agonist-specific effects of guanine nucleotides on alpha-adrenergic receptors in human platelets. 
  • Altered activity of the nucleotide regulatory site in the parathyroid hormone-sensitive adenylate cyclase from the renal cortex of a patient with pseudohypoparathyroidism. 
  • Analysis of the neurofibromatosis type 1 (NF1) GAP-related domain by site-directed mutagenesis. 
  • Antitumor agents. 139. Synthesis and biological evaluation of thiocolchicine analogs 5,6-dihydro-6(S)-(acyloxy)- and 5,6-dihydro-6(S)-[(aroyloxy)methyl]-1,2,3-trimethoxy-9-(methylthio)-8H- cyclohepta[a]naphthalen-8-ones as novel cytotoxic and antimitotic agents. 
  • Apparent cooperative assembly of the bacterial cell division protein FtsZ demonstrated by isothermal titration calorimetry. 
  • Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. 
  • Assembly dynamics of Mycobacterium tuberculosis FtsZ. 
  • Assembly of pure tubulin in the absence of free GTP: effect of magnesium, glycerol, ATP, and the nonhydrolyzable GTP analogues. 
  • Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p. 
  • Bacterial cell division protein FtsZ assembles into protofilament sheets and minirings, structural homologs of tubulin polymers. 
  • Beta-adrenergic inhibition of cardiac sodium channels by dual G-protein pathways. 
  • Beta-adrenergic receptor overexpression in the fetal rat: distribution, receptor subtypes, and coupling to adenylate cyclase activity via G-proteins. 
  • Beta-adrenoceptor control of cardiac adenylyl cyclase during development: agonist pretreatment in the neonate uniquely causes heterologous sensitization, not desensitization. 
  • Binding of rat alpha 1-inhibitor-3-methylamine to the alpha 2-macroglobulin signaling receptor induces second messengers. 
  • Biochemical and immunological abnormalities in purine nucleoside phosphorylase deficient mice. 
  • BtubA-BtubB heterodimer is an essential intermediate in protofilament assembly. 
  • C-Terminal glycine-gated radical initiation by GTP 3',8-cyclase in the molybdenum cofactor biosynthesis. 
  • Calcium regulates S-nitrosylation, denitrosylation, and activity of tissue transglutaminase. 
  • Catecholamine stimulated myocardial adenylate cyclase: effects of nucleotides. 
  • Cdc42p, GTP hydrolysis, and the cell's sense of direction. 
  • Cellular mechanisms for developmental toxicity of chlorpyrifos: targeting the adenylyl cyclase signaling cascade. 
  • Characteristics of 5'-guanylyl imidodiphosphate-activated adenylate cyclase. 
  • Characteristics of GTP-mediated microsomal Ca2+ release. 
  • Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. 
  • Characterization of two different five-coordinate soluble guanylate cyclase ferrous-nitrosyl complexes. 
  • Clinical physiology of adrenergic receptor regulation. 
  • Coat proteins and selective protein packaging into transport vesicles. 
  • Commentary. Molecular pharmacology of beta-adrenergic receptors--a status report. 
  • Complete hematologic and cytogenetic response to 2-amino-9-beta-D-arabinosyl-6-methoxy-9H-guanine in a patient with chronic myelogenous leukemia in T-cell blastic phase: a case report and review of the literature. 
  • Compound GW506U78 in refractory hematologic malignancies: relationship between cellular pharmacokinetics and clinical response. 
  • Coordinate regulation of forskolin-induced cellular proliferation in macrophages by protein kinase A/cAMP-response element-binding protein (CREB) and Epac1-Rap1 signaling: effects of silencing CREB gene expression on Akt activation. 
  • Correlation of beta-adrenergic receptor-stimulated [3H]GDP release and adenylate cyclase activation. Differences between frog and turkey erythrocyte membranes. 
  • Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap. 
  • Cyclocreatine (1-carboxymethyl-2-iminoimidazolidine) inhibits the replication of human herpes viruses. 
  • DNA repair protein O6-alkylguanine-DNA alkyltransferase is phosphorylated by two distinct and novel protein kinases in human brain tumour cells. 
  • Definition of a consensus transportin-specific nucleocytoplasmic transport signal. 
  • Dependence of leucine-rich repeat kinase 2 (LRRK2) kinase activity on dimerization. 
  • Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides. 
  • Differential effects of GTP on the coupling of beta-adrenergic receptors to adenylate cyclase from frog and turkey erythrocytes. Application of new methods for the analysis of receptor-effector coupling. 
  • Dilution-induced disassembly of microtubules: relation to dynamic instability and the GTP cap. 
  • Direct evidence that Gi-coupled receptor stimulation of mitogen-activated protein kinase is mediated by G beta gamma activation of p21ras. 
  • Dopaminergic inhibition of adenylate cyclase correlates with high affinity agonist binding to anterior pituitary D2 dopamine receptors. 
  • Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. 
  • Effects of magnesium on the dynamic instability of individual microtubules. 
  • Endogenous proteinases modulate the function of the beta-adrenergic receptor-adenylate cyclase system. 
  • Endothelin activates voltage-dependent Ca2+ current by a G protein-dependent mechanism in rabbit cardiac myocytes. 
  • Enhanced adenylate cyclase activity of turkey erythrocytes following treatment with beta-adrenergic receptor antagonists. 
  • Enzymology of GTPase acceleration in phototransduction. 
  • Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. 
  • Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion. 
  • Evidence that ribavirin is transported in Aedes albopictus cells by the nucleoside transport system. 
  • Expression of cDNAs for G proteins in Escherichia coli. Two forms of Gs alpha stimulate adenylate cyclase. 
  • FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis. 
  • FtsZ from Escherichia coli, Azotobacter vinelandii, and Thermotoga maritima--quantitation, GTP hydrolysis, and assembly. 
  • Functional differences in the beta gamma complexes of transducin and the inhibitory guanine nucleotide regulatory protein. 
  • G protein involvement in receptor-effector coupling. 
  • G proteins in medicine. 
  • GTP hydrolysis during microtubule assembly. 
  • GTP-mediated Ca2+ release in rough endoplasmic reticulum. Correlation with a GTP-sensitive increase in membrane permeability. 
  • Glucocorticoid-targeting of the adenylyl cyclase signaling pathway in the cerebellum of young vs. aged rats. 
  • Glucocorticoids enhance intracellular signaling via adenylate cyclase at three distinct loci in the fetus: a mechanism for heterologous teratogenic sensitization? 
  • Glucocorticoids regulate the development of intracellular signaling: enhanced forebrain adenylate cyclase catalytic subunit activity after fetal dexamethasone exposure. 
  • Guanine arabinoside as a bone marrow-purging agent. 
  • Guanine nucleotides modulate the binding affinity of the oligopeptide chemoattractant receptor on human polymorphonuclear leukocytes. 
  • Guanine nucleotides regulate both dopaminergic agonist and antagonist binding in porcine anterior pituitary. 
  • Guanosine triphosphate binding sites in solubilized myocardium. Relation to adenylate cyclase activity. 
  • Guanosinetriphosphatase activity of tubulin associated with microtubule assembly. 
  • Gz, a guanine nucleotide-binding protein with unique biochemical properties. 
  • Heterogeneity of radioligand binding to alpha-adrenergic receptors. Analysis of guanine nucleotide regulation of agonist binding in relation to receptor subtypes. 
  • Homologous desensitization of the beta-adrenergic receptor. Functional integrity of the desensitized receptor from mammalian lung. 
  • Hormonal inhibition of adenylate cyclase. alpha 2 Adrenergic receptors promote release of [3H]guanylylimidodiphosphate from platelet membranes. 
  • Hormone-sensitive adenylate cyclase. Delineation of a trypsin-sensitive site in the pathway of receptor-mediated inhibition. 
  • Human substance P receptor expressed in Chinese hamster ovary cells directly activates G(alpha q/11), G(alpha s), G(alpha o). 
  • Identification and characterization of a leucine-rich repeat kinase 2 (LRRK2) consensus phosphorylation motif. 
  • Identification and initial characterization of translational initiation factor 2 from bovine mitochondria. 
  • Identification of chemical inhibitors to human tissue transglutaminase by screening existing drug libraries. 
  • Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets. 
  • In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB. 
  • In vitro assembly studies of FtsZ/tubulin-like proteins (TubZ) from Bacillus plasmids: evidence for a capping mechanism. 
  • Incorporation of ribonucleoside 5'-(alpha-P-borano)triphosphates into a 20-mer RNA by T7 RNA polymerase. 
  • Incorporation of tyrosine and glutamine residues into the soluble guanylate cyclase heme distal pocket alters NO and O2 binding. 
  • Inhibition of cardiac Na+ currents by isoproterenol. 
  • Inosine triphosphate protects against ribavirin-induced adenosine triphosphate loss by adenylosuccinate synthase function. 
  • Inside-out Z rings--constriction with and without GTP hydrolysis. 
  • Interaction sites of the COOH-terminal region of the gamma subunit of cGMP phosphodiesterase with the GTP-bound alpha subunit of transducin. 
  • Irreversible activation of adenylate cyclase of toad erythrocyte plasma membrane by 5'-guanylylimidodiphosphate. 
  • Irreversible stimulation of adenylate cyclase activity of fat cell membranes of phosphoramidate and phosphonate analogs of GTP. 
  • Isobutylmethylxanthine stimulates adenylate cyclase by blocking the inhibitory regulatory protein, Gi. 
  • Isolation of GTP-binding proteins from myeloid HL-60 cells. Identification of two pertussis toxin substrates. 
  • Kinetics of irreversible activation of adenylate cyclase of fat cell membranes by phosphonium and phosphoramidate analogs of gtp1. 
  • LRRK2 autophosphorylation enhances its GTPase activity. 
  • LRRK2 phosphorylates membrane-bound Rabs and is activated by GTP-bound Rab7L1 to promote recruitment to the trans-Golgi network. 
  • Ligation of the alpha2M signalling receptor elevates the levels of p21Ras-GTP in macrophages. 
  • Mechanism of action of Vibrio cholerae enterotoxin. Effects on adenylate cyclase of toad and rat erythrocyte plasma membranes. 
  • Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin. Relations to the mode of activation by hormones. 
  • Mechanism of guanine nucleotide regulatory protein-mediated inhibition of adenylate cyclase. Studies with isolated subunits of transducin in a reconstituted system. 
  • Mechanisms of hormone receptor-effector coupling: the beta-adrenergic receptor and adenylate cyclase. 
  • Microinjection of a 19-kDa guanine nucleotide-binding protein inhibits maturation of Xenopus oocytes. 
  • Microtubule dynamic instability and GTP hydrolysis. 
  • Modeling the physics of FtsZ assembly and force generation. 
  • Molecular determinants of selectivity in 5-hydroxytryptamine1B receptor-G protein interactions. 
  • Molecular pharmacology of adenylate cyclase-coupled alpha- and beta-adrenergic receptors. 
  • Multiple cyclic AMP receptors are linked to adenylyl cyclase in Dictyostelium. 
  • Multiple effects of guanine nucleotides on human platelet adenylated cyclase. 
  • Mutation-specific RAS oncogenicity explains NRAS codon 61 selection in melanoma. 
  • Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1. 
  • Myocardial guanylate cyclase: properties of the enzyme and effects of cholinergic agonists in vitro. 
  • Nascent secretory chain binding and translocation are distinct processes: differentiation by chemical alkylation. 
  • Nitric oxide regulates endocytosis by S-nitrosylation of dynamin. 
  • Novel pathogenic LRRK2 p.Asn1437His substitution in familial Parkinson's disease. 
  • Nucleotide regulation of soluble guanylate cyclase substrate specificity. 
  • Nucleotide regulatory protein-mediated activation of phospholipase C in human polymorphonuclear leukocytes is disrupted by phorbol esters. 
  • Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. 
  • On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision. 
  • One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity. 
  • Ontogeny of regulatory mechanisms for beta-adrenoceptor control of rat cardiac adenylyl cyclase: targeting of G-proteins and the cyclase catalytic subunit. 
  • Pharmacologic purging of malignant T cells from human bone marrow using 9-beta-D-arabinofuranosylguanine. 
  • Phosphorylation of the beta-adrenergic receptor in intact cells: relationship to heterologous and homologous mechanisms of adenylate cyclase desensitization. 
  • Polycation-induced assembly of purified tubulin. 
  • Polyethylene glycol-stimulated microsomal GTP hydrolysis. Relationship to GTP-mediated Ca2+ release. 
  • Polymerization of Ftsz, a bacterial homolog of tubulin. is assembly cooperative? 
  • Pregnancy and estradiol modulate myometrial G-protein pathways in the guinea pig. 
  • Purification and assembly of FtsZ. 
  • Purification and characterization of GIT family of ADP-ribosylation factor (ARF) GTPase-activating proteins. 
  • Purification and characterization of the D2-dopamine receptor from bovine anterior pituitary. 
  • Purine nucleoside phosphorylase deficiency. 
  • RGS10 is a selective activator of G alpha i GTPase activity. 
  • RGSZ1, a Gz-selective regulator of G protein signaling whose action is sensitive to the phosphorylation state of Gzalpha. 
  • Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells. 
  • Rab5 association with the angiotensin II type 1A receptor promotes Rab5 GTP binding and vesicular fusion. 
  • Radical Breakthroughs in Natural Product and Cofactor Biosynthesis. 
  • Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer. 
  • Rapid in vitro assembly of Caulobacter crescentus FtsZ protein at pH 6.5 and 7.2. 
  • Reconstitution of a hormone-sensitive adenylate cyclase system. The pure beta-adrenergic receptor and guanine nucleotide regulatory protein confer hormone responsiveness on the resolved catalytic unit. 
  • Reconstitution of nuclear protein export in isolated nuclear envelopes. 
  • Regulation of beta-adrenergic receptors by guanyl-5'-yl imidodiphosphate and other purine nucleotides. 
  • Regulation of deactivation of photoreceptor G protein by its target enzyme and cGMP. 
  • Regulation of hormone receptors and adenylyl cyclases by guanine nucleotide binding N proteins. 
  • Regulation of mitogen-activated protein kinase pathways by catecholamine receptors. 
  • Regulation of neuroendocrine exocytosis by the ARF6 GTPase-activating protein GIT1. 
  • Regulation of transducin GTPase activity in bovine rod outer segments. 
  • Repetitive episodes of brief ischaemia (12 min) do not produce a cumulative depletion of high energy phosphate compounds. 
  • Rodbell and Gilman win 1994 Nobel Prize for Physiology and Medicine. 
  • Role for the target enzyme in deactivation of photoreceptor G protein in vivo. 
  • Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization. 
  • SU5416 delays wound healing through inhibition of TGF-beta 1 activation. 
  • Scaffold-mediated symmetry breaking by Cdc42p. 
  • Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing. 
  • Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p. 
  • Site-specific mutations of FtsZ--effects on GTPase and in vitro assembly. 
  • Sites of circularization of the Tetrahymena rRNA IVS are determined by sequence and influenced by position and secondary structure. 
  • Somatostatin selectively couples to G(o) alpha in HIT-T15 cells. 
  • Specificity of receptor-G protein interactions. Discrimination of Gi subtypes by the D2 dopamine receptor in a reconstituted system. 
  • Specificity of the functional interactions of the beta-adrenergic receptor and rhodopsin with guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles. 
  • Stimulation of catecholamine-sensitive adenylate cyclase by 5'-guanylyl-imidodiphosphate. 
  • Straight and curved conformations of FtsZ are regulated by GTP hydrolysis. 
  • Structural and functional studies of cyclic GMP phosphodiesterase. 
  • Structural and signaling requirements for BCR-ABL-mediated transformation and inhibition of apoptosis. 
  • Subtype-specific binding of azidoanilido-GTP by purified G protein alpha subunits. 
  • Synthesis and evaluation of a phosphonate analogue of the soluble guanylate cyclase activator YC-1. 
  • The 2.0 A crystal structure of a heterotrimeric G protein. 
  • The Chloroplast Tubulin Homologs FtsZA and FtsZB from the Red Alga Galdieria sulphuraria Co-assemble into Dynamic Filaments. 
  • The D2-dopamine receptor of anterior pituitary is functionally associated with a pertussis toxin-sensitive guanine nucleotide binding protein. 
  • The E2-like conjugation enzyme Atg3 promotes binding of IRG and Gbp proteins to Chlamydia- and Toxoplasma-containing vacuoles and host resistance. 
  • The Lipid Kinase PI5P4Kβ Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis. 
  • The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro. 
  • The formylpeptide chemoattractant receptor copurifies with a GTP-binding protein containing a distinct 40-kDa pertussis toxin substrate. 
  • The hepatic glucagon receptor: a comparative study of the regulatory and structural properties. 
  • The inducibly expressed GTPase localizes to the endoplasmic reticulum, independently of GTP binding. 
  • The kinetics of microtubule assembly. Evidence for a two-stage nucleation mechanism. 
  • The new cardiotonic agent sulmazole is an A1 adenosine receptor antagonist and functionally blocks the inhibitory regulator, Gi. 
  • The straight and curved conformation of FtsZ protofilaments-evidence for rapid exchange of GTP into the curved protofilament. 
  • The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase. 
  • The structure and assembly of microtubules. 
  • Ubiquitination of K-Ras enhances activation and facilitates binding to select downstream effectors. 
  • Unique uncoupling of the frog erythrocyte adenylate cyclase system by manganese. Loss of hormone and guanine nucleotide-sensitive enzyme activities without loss of nucleotide-sensitive, high affinity agonist binding. 
  • Using Cryo-EM to Map Small Ligands on Dynamic Metabolic Enzymes: Studies with Glutamate Dehydrogenase. 
  • Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. 
  • ZipA and FtsA* stabilize FtsZ-GDP miniring structures. 
  • beta-Arrestin-mediated ADP-ribosylation factor 6 activation and beta 2-adrenergic receptor endocytosis. 
  • cGMP suppresses GTPase activity of a portion of transducin equimolar to phosphodiesterase in frog rod outer segments. Light-induced cGMP decreases as a putative feedback mechanism of the photoresponse. 
• 

  Keywords of People

  • Bennett, Vann, George B. Geller Distinguished Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute
  • Erickson, Harold Paul, James B. Duke Distinguished Professor of Cell Biology, Biomedical Engineering
  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology
  • Taylor, Gregory Alan, Professor in Medicine, Immunology