Hydrolysis

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  Subject Areas on Research

  • 5-HT1A and histamine H1 receptors in HeLa cells stimulate phosphoinositide hydrolysis and phosphate uptake via distinct G protein pools. 
  • 8-hydroxy-2-(di-n-propylamino)tetralin-responsive 5-hydroxytryptamine4-like receptor expressed in bovine pulmonary artery smooth muscle cells. 
  • A THERMODYNAMIC STUDY OF THE HYDROLYSIS OF CYTIDINE 2',3'-CYCLIC PHOSPHATE. 
  • A calorimetric and equilibrium investigation of the hydrolysis of lactose. 
  • A defined human system that supports bidirectional mismatch-provoked excision. 
  • A general and efficient cantilever functionalization technique for AFM molecular recognition studies. 
  • A kinesin motor in a force-producing conformation. 
  • A mathematical model for the receptor mediated cellular regulation of the low density lipoprotein metabolism. 
  • A new structural state of myosin. 
  • A pharmacodynamic model to investigate the structure-activity profile of a series of novel opioid analgesics. 
  • A plasma membrane-associated component of ovarian adenocarcinoma cells enhances the catalytic efficiency of matrix metalloproteinase-2. 
  • A proteomic analysis of human cilia: identification of novel components. 
  • A rapid fluorescence assay for FtsZ assembly indicates cooperative assembly with a dimer nucleus. 
  • A structural basis for a phosphoramide mustard-induced DNA interstrand cross-link at 5'-d(GAC). 
  • A structural comparison of tryptic fragments of three types of intermediate filaments. 
  • ATP hydrolysis by a CFTR domain: pharmacology and effects of G551D mutation. 
  • Abnormalities in S-adenosylhomocysteine hydrolysis, ATP catabolism, and lymphoid differentiation in adenosine deaminase deficiency. 
  • Acid- and base-catalyzed hydrolysis of chloroacetamide herbicides. 
  • Activation of the sphingomyelin cycle by brefeldin A: effects of brefeldin A on differentiation and implications for a role for ceramide in regulation of protein trafficking. 
  • Acylation of naturally occurring and synthetic 1-deoxysphinganines by ceramide synthase. Formation of N-palmitoyl-aminopentol produces a toxic metabolite of hydrolyzed fumonisin, AP1, and a new category of ceramide synthase inhibitor. 
  • Adenosine nucleotides and the regulation of GRP94-client protein interactions. 
  • Adenylate cyclase-coupled beta adrenergic receptors effect of membrane lipid-perturbing agents on receptor binding and enzyme stimulation by catecholamines. 
  • Akt-directed glucose metabolism can prevent Bax conformation change and promote growth factor-independent survival. 
  • Alpha 2-macroglobulin: a sensor for proteolysis. 
  • Alpha-fodrin is cleaved by caspase-3 in a chronic ocular hypertensive (COH) rat model of glaucoma. 
  • An x-ray study of the effect of enzymes on frog sciatic nerve myelin. 
  • Analysis of thiolester bond cleavage-dependent conformational changes in binary alpha 2-macroglobulin-proteinase complexes. 
  • Antigenic, chemical, and structural properties of cell walls of Histoplasma capsulatum yeast-form chemotypes 1 and 2 after serial enzymatic hydrolysis. 
  • Automated analysis for free and short-chain acylcarnitine in plasma with a centrifugal analyzer. 
  • Auxin action: slogging out of the swamp. 
  • Berninamycin. 2. Products of acidic hydrolysis, methanolysis, and acetolysis of berninamycin A1. 
  • Boranophosphates support the RNase H cleavage of polyribonucleotides. 
  • Brefeldin A promotes hydrolysis of sphingomyelin. 
  • BtubA-BtubB heterodimer is an essential intermediate in protofilament assembly. 
  • Cdc42p, GTP hydrolysis, and the cell's sense of direction. 
  • Ceramides and other bioactive sphingolipid backbones in health and disease: lipidomic analysis, metabolism and roles in membrane structure, dynamics, signaling and autophagy. 
  • Characterization and application of an avian in vitro spermatozoa-egg interaction assay using the inner perivitelline layer from laid chicken eggs. 
  • Characterization of corneal proteoglycans under vitamin A deficiency. 
  • Characterization of senescence- and apoptosis-dependent forms of terminin as derived from a precursor found in replicating and nonreplicating cells. 
  • Chasing acyl carrier protein through a catalytic cycle of lipid A production. 
  • Chemoattractant receptor-induced hydrolysis of phosphatidylinositol 4,5-bisphosphate in human polymorphonuclear leukocyte membranes. Requirement for a guanine nucleotide regulatory protein. 
  • Cholesterol interaction with the daunorubicin binding site of P-glycoprotein. 
  • Cholinergic stimulation of phosphatidylinositol hydrolysis by rat corneal epithelium in vitro. 
  • Chromatographic analysis and decomposition product characterization of compound SR16157 (NSC 732011). 
  • Cleavage of oligoribonucleotides by a ribozyme derived from the hepatitis delta virus RNA sequence. 
  • Comparison of HPLC/ESI-FTICR MS versus MALDI-TOF/TOF MS for glycopeptide analysis of a highly glycosylated HIV envelope glycoprotein. 
  • Control of electron transfer in nitrogenase. 
  • Conversion of endogenous indole-3-butyric acid to indole-3-acetic acid drives cell expansion in Arabidopsis seedlings. 
  • Crustacean peptide and peptide-like pheromones and kairomones. 
  • Cryo-EM Analysis of the Conformational Landscape of Human P-glycoprotein (ABCB1) During its Catalytic Cycle. 
  • Daily oral consumption of hydrolyzed type 1 collagen is chondroprotective and anti-inflammatory in murine posttraumatic osteoarthritis. 
  • Degradation of proteoglycan in articular cartilage. 
  • Deletion of the glutamate carboxypeptidase II gene in mice reveals a second enzyme activity that hydrolyzes N-acetylaspartylglutamate. 
  • Delivery of antisense oligonucleotides using HPMA polymer: synthesis of A thiol polymer and its conjugation to water-soluble molecules. 
  • Deoxycholate induces the preferential hydrolysis of polyphosphoinositides by human platelet and rat corneal phospholipase C. 
  • Differential and simultaneous adenosine di- and triphosphate binding by MutS. 
  • Differential regulation of formyl peptide and platelet-activating factor receptors. Role of phospholipase Cbeta3 phosphorylation by protein kinase A. 
  • Differential regulation of proteasome function in isoproterenol-induced cardiac hypertrophy. 
  • Differential specificities and simultaneous occupancy of human MutSalpha nucleotide binding sites. 
  • Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha. 
  • Discovery and characterization of human antibody inhibitors of pregnancy-associated plasma protein-A. 
  • Discovery of Six Ramoplanin Family Gene Clusters and the Lipoglycodepsipeptide Chersinamycin*. 
  • Discrete amino acid sequences of the alpha 1-adrenergic receptor determine the selectivity of coupling to phosphatidylinositol hydrolysis. 
  • Dissecting the order of bacteriophage T4 DNA polymerase holoenzyme assembly. 
  • Distinct MutS DNA-binding modes that are differentially modulated by ATP binding and hydrolysis. 
  • Durhamycin A, a potent inhibitor of HIV Tat transactivation. 
  • Effector coupling mechanisms of the cloned 5-HT1A receptor. 
  • Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis. 
  • Effects of bacterial lipopolysaccharide on the hydrolysis of phosphatidylinositol-4,5-bisphosphate in murine peritoneal macrophages. 
  • Energy Transduction in Nitrogenase. 
  • Enzymatic Synthesis of Nucleobase-Modified Single-Stranded DNA Offers Tunable Resistance to Nuclease Degradation. 
  • Enzymology of GTPase acceleration in phototransduction. 
  • Evaluating the bioaccessibility of flame retardants in house dust using an in vitro Tenax bead-assisted sorptive physiologically based method. 
  • Evidence for an electrostatic mechanism of force generation by the bacteriophage T4 DNA packaging motor. 
  • Evidence that CCK-58 has structure that influences its biological activity. 
  • Evidence that a catalytic glutamate and an 'Arginine Toggle' act in concert to mediate ATP hydrolysis and mechanochemical coupling in a viral DNA packaging motor. 
  • Extensive hydrolysis of N-formyl-L-methionyl-L-leucyl-L-[3H] phenylalanine by human polymorphonuclear leukocytes. A potential mechanism for modulation of the chemoattractant signal. 
  • Facilitated diffusion during catalysis by EcoRI endonuclease. Nonspecific interactions in EcoRI catalysis. 
  • Factors in standardizing automated cholinesterase assays. 
  • FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis. 
  • FtsZ from Escherichia coli, Azotobacter vinelandii, and Thermotoga maritima--quantitation, GTP hydrolysis, and assembly. 
  • Functions of MutLalpha, replication protein A (RPA), and HMGB1 in 5'-directed mismatch repair. 
  • Further insights into quinone cofactor biogenesis: probing the role of mauG in methylamine dehydrogenase tryptophan tryptophylquinone formation. 
  • GTP hydrolysis during microtubule assembly. 
  • GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1. 
  • Human mismatch repair: reconstitution of a nick-directed bidirectional reaction. 
  • Hydrolysis by somatic angiotensin-I converting enzyme of basic dipeptides from a cholecystokinin/gastrin and a LH-RH peptide extended at the C-terminus with gly-Arg/Lys-arg, but not from diarginyl insulin. 
  • Hydrolysis of N3-methyl-2'-deoxycytidine: model compound for reactivity of protonated cytosine residues in DNA. 
  • Hydrolysis of template and newly synthesized deoxyribonucleic acid by the 3' to 5' exonuclease activity of the T4 deoxyribonucleic acid polymerase. 
  • Hydrolytic function of Exo1 in mammalian mismatch repair. 
  • Hydrolytically deficient MutS E694A is defective in the MutL-dependent activation of MutH and in the mismatch-dependent assembly of the MutS.MutL.heteroduplex complex. 
  • Hydrolyzed fumonisins HFB1 and HFB2 are acylated in vitro and in vivo by ceramide synthase to form cytotoxic N-acyl-metabolites. 
  • Identification and characterization of a leucine-rich repeat kinase 2 (LRRK2) consensus phosphorylation motif. 
  • Identification of arachidonic acid as a mediator of sphingomyelin hydrolysis in response to tumor necrosis factor alpha. 
  • Identification of novel predictive markers for the prognosis of pancreatic ductal adenocarcinoma. 
  • Identification of the second chromophore of Escherichia coli and yeast DNA photolyases as 5,10-methenyltetrahydrofolate. 
  • Improved speciation of dissolved organic nitrogen in natural waters: amide hydrolysis with fluorescence derivatization. 
  • In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB. 
  • In vitro proteolysis of brain spectrin by calpain I inhibits association of spectrin with ankyrin-independent membrane binding site(s). 
  • In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4. 
  • Independent analysis of bait region cleavage dependent and thiolester bond cleavage dependent conformational changes by cross-linking of alpha 2-macroglobulin with cis-dichlorodiammineplatinum(II) and dithiobis(succinimidyl propionate). 
  • Inhibition of muscarinic-coupled phosphoinositide hydrolysis by N-methyl-D-aspartate is dependent on depolarization via channel activation. 
  • Inhibition of rat brain phosphatidylinositol-specific phospholipase C by aluminum: regional differences, interactions with aluminum salts, and mechanisms. 
  • Initiation of methyl-directed mismatch repair. 
  • Inositol diphosphate signaling regulates telomere length. 
  • Inside-out Z rings--constriction with and without GTP hydrolysis. 
  • Interleukin-1 stimulates diacylglycerol production in T lymphocytes by a novel mechanism. 
  • Interplay of catalysis, fidelity, threading, and processivity in the exo- and endonucleolytic reactions of human exonuclease I. 
  • Intracellular proteolytic cleavage of 9-cis-retinoic acid receptor alpha by cathepsin L-type protease is a potential mechanism for modulating thyroid hormone action. 
  • Intrinsic GTP hydrolysis is observed for a switch 1 variant of Cdc42 in the presence of a specific GTPase inhibitor. 
  • Investigating the Sensitivity of NAD+-dependent Sirtuin Deacylation Activities to NADH. 
  • Keap1 loss promotes Kras-driven lung cancer and results in dependence on glutaminolysis. 
  • Kinesin motors as molecular machines. 
  • Kinetic characterization of Salmonella FliK-FlhB interactions demonstrates complexity of the Type III secretion substrate-specificity switch. 
  • Kinetic mechanism of the DNA-dependent DNA polymerase activity of human immunodeficiency virus reverse transcriptase. 
  • Kinetic scheme for intermolecular RNA cleavage by a ribozyme derived from hepatitis delta virus RNA. 
  • Ligand interactions in the adenosine nucleotide-binding domain of the Hsp90 chaperone, GRP94. I. Evidence for allosteric regulation of ligand binding. 
  • Lipid modification gives rise to two distinct Haloferax volcanii S-layer glycoprotein populations. 
  • Macrophage activation downregulates the degradative capacity of the phagosome. 
  • Mapping the ankyrin-binding site of the human erythrocyte anion exchanger. 
  • Mechanism for catalysis and regulation of adenosine 5'-triphosphate hydrolysis by chloroplast coupling factor 1. 
  • Mechanism of hypochlorite-mediated inactivation of proteinase inhibition by alpha 2-macroglobulin. 
  • Mechanisms in eukaryotic mismatch repair. 
  • Membrane topology and biogenesis of eukaryotic signal peptidase. 
  • Mice deficient in endothelin-converting enzyme-2 exhibit abnormal responses to morphine and altered peptide levels in the spinal cord. 
  • Microtubule dynamic instability and GTP hydrolysis. 
  • Modifying aroylhydrazone prochelators for hydrolytic stability and improved cytoprotection against oxidative stress. 
  • Modulation of MutS ATP hydrolysis by DNA cofactors. 
  • Molecular mechanical properties of short-sequence peptide enzyme mimics. 
  • Monitoring β-secretase activity in living cells with a membrane-anchored FRET probe. 
  • Multiple and specific initiation of T4 DNA replication. 
  • Multiple second messenger pathways of alpha-adrenergic receptor subtypes expressed in eukaryotic cells. 
  • Mutational analysis of the lysyl hydroxylase 1 gene (PLOD) in six unrelated patients with Ehlers-Danlos syndrome type VI: prenatal exclusion of this disorder in one family. 
  • New lessons in the regulation of glucose metabolism taught by the glucose 6-phosphatase system. 
  • Nitric oxide-dependent ribosomal RNA cleavage is associated with inhibition of ribosomal peptidyl transferase activity in ANA-1 murine macrophages. 
  • Noncatalytic domains of RGS9-1.Gbeta 5L play a decisive role in establishing its substrate specificity. 
  • Nonproteolytic incorporation of protein ligands into human alpha 2-macroglobulin: implications for the binding mechanism of alpha 2-macroglobulin. 
  • Norethynodrel metabolites in human plasma and urine. 
  • Novel protein purification system utilizing an N-terminal fusion protein and a caspase-3 cleavable linker. 
  • Optical nanobiosensor for monitoring an apoptotic signaling process in a single living cell following photodynamic therapy. 
  • Overexpression of G protein-coupled receptor kinase-2 in smooth muscle cells attenuates mitogenic signaling via G protein-coupled and platelet-derived growth factor receptors. 
  • Overexpression of protein targeting to glycogen (PTG) in rat hepatocytes causes profound activation of glycogen synthesis independent of normal hormone- and substrate-mediated regulatory mechanisms. 
  • Oxidized low density lipoprotein suppresses activation of NF kappa B in macrophages via a pertussis toxin-sensitive signaling mechanism. 
  • Plasma 1-palmitoyl-2-linoleoyl phosphatidylcholine. Evidence for extensive phospholipase A1 hydrolysis and hepatic metabolism of the products. 
  • Polyethylene glycol-stimulated microsomal GTP hydrolysis. Relationship to GTP-mediated Ca2+ release. 
  • Predicted group II intron lineages E and F comprise catalytically active ribozymes. 
  • Preparative solvent partition chromatography of butyrylated cyclic AMP analogues. 
  • Presenilin-dependent gamma-secretase-like intramembrane cleavage of ErbB4. 
  • Probing conformational changes in human DNA topoisomerase IIα by pulsed alkylation mass spectrometry. 
  • Prostaglandin F2 alpha receptors in the human trabecular meshwork. 
  • Protein kinase A regulates ATP hydrolysis and dimerization by a CFTR (cystic fibrosis transmembrane conductance regulator) domain. 
  • Proteinase binding and inhibition by the monomeric alpha-macroglobulin rat alpha 1-inhibitor-3. 
  • Proteolysis of smooth muscle myosin light chain kinase. Formation of inactive and calmodulin-independent fragments. 
  • Pseudocholinesterase activity in human cerebrospinal fluid. 
  • Purification and partial characterization of a thiol proteinase from the thermophilic fungus Humicola lanuginosa. 
  • Purification of glycopeptides of human plasma proteins by high-performance liquid chromatography. 
  • Quantitative analysis of twelve sulfonamides in honey after acidic hydrolysis by high-performance liquid chromatography with post-column derivatization and fluorescence detection. 
  • RGS10 is a selective activator of G alpha i GTPase activity. 
  • RGS4 inhibits platelet-activating factor receptor phosphorylation and cellular responses. 
  • Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells. 
  • Rac1, a low-molecular-mass GTP-binding-protein with high intrinsic GTPase activity and distinct biochemical properties. 
  • Rapid in vitro assembly of Caulobacter crescentus FtsZ protein at pH 6.5 and 7.2. 
  • Reaper is regulated by IAP-mediated ubiquitination. 
  • Receptor-mediated endocytosis of carcinoembryonic antigen by rat alveolar macrophages in vitro. 
  • Regions of the alpha 1-adrenergic receptor involved in coupling to phosphatidylinositol hydrolysis and enhanced sensitivity of biological function. 
  • Regulation and function of the muscle glycogen-targeting subunit of protein phosphatase 1 (GM) in human muscle cells depends on the COOH-terminal region and glycogen content. 
  • Regulation of hormone receptors and adenylyl cyclases by guanine nucleotide binding N proteins. 
  • Regulation of transducin GTPase activity in bovine rod outer segments. 
  • Rod outer segment structure influences the apparent kinetic parameters of cyclic GMP phosphodiesterase. 
  • Role for cytoplasmic nucleotide hydrolysis in hepatic function and protein synthesis. 
  • Role for the target enzyme in deactivation of photoreceptor G protein in vivo. 
  • Role of decreased levels of lipid phosphate phosphatase-1 in accumulation of lysophosphatidic acid in ovarian cancer. 
  • Role of granulocyte collagenase in collagen degradation. 
  • Scaffold-mediated symmetry breaking by Cdc42p. 
  • Selective inhibition of heterotrimeric Gs signaling. Targeting the receptor-G protein interface using a peptide minigene encoding the Galpha(s) carboxyl terminus. 
  • Self-cleaving ribozymes of hepatitis delta virus RNA. 
  • Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p. 
  • Sequential quadratic programming method for determining the minimum energy path. 
  • Short-chain alkyl esters of L-dopa as prodrugs for rectal absorption. 
  • Sodium binding site of factor Xa: role of sodium in the prothrombinase complex. 
  • Soft-lithographic approach to functionalization and nanopatterning oxide-free silicon. 
  • Stimulation of phosphorylcholine turnover and diacylglycerol production in human polymorphonuclear leukocytes. Novel assay for phosphorylcholine. 
  • Straight and curved conformations of FtsZ are regulated by GTP hydrolysis. 
  • Strain variation in composition and molecular size of the capsular polysaccharide of Cryptococcus neoformans serotype A. 
  • Structure and biosynthesis of free lipid A molecules that replace lipopolysaccharide in Francisella tularensis subsp. novicida. 
  • Structure and function of the UvrB protein. 
  • Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis. 
  • Structure of the major peanut allergen Ara h 1 may protect IgE-binding epitopes from degradation. 
  • Studies of the phosphoenzyme intermediate of the yeast plasma membrane proton-translocating ATPase. 
  • Substrate Selectivity of Lysophospholipid Transporter LplT Involved in Membrane Phospholipid Remodeling in Escherichia coli. 
  • Surfactant-assisted synthesis of Keggin-type polyoxometalates nanorods. 
  • Synthesis and characterization of carbohydrate-based phospholipids. 
  • The Lipid Kinase PI5P4Kβ Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis. 
  • The MutL ATPase is required for mismatch repair. 
  • The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation. 
  • The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro. 
  • The evaluation of complex-dependent alterations in human factor VIIa. 
  • The farnesyl protein transferase inhibitor SCH66336 is a potent inhibitor of MDR1 product P-glycoprotein. 
  • The hydrolytic susceptibility of prochelator BSIH in aqueous solutions. 
  • The identification and characterization of a G4-DNA resolvase activity. 
  • The standardization of the thiobarbituric acid assay for nonenzymatic glucosylation of human serum albumin. 
  • The structure of helical lipoprotein lipase reveals an unexpected twist in lipase storage. 
  • Three Pyrimidine Decarboxylations in the Absence of a Catalyst. 
  • Transducin GTPase provides for rapid quenching of the cGMP cascade in rod outer segments. 
  • Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds 
  • Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro. 
  • Uncoupling of nucleotide hydrolysis and polymerization in the ParA protein superfamily disrupts DNA segregation dynamics. 
  • Unifying concept for the coupling between ion pumping and ATP hydrolysis or synthesis. 
  • YbiV from Escherichia coli K12 is a HAD phosphatase. 
  • Yeast screens identify the RNA polymerase II CTD and SPT5 as relevant targets of BRCA1 interaction. 
  • beta-Arrestin scaffolding of the ERK cascade enhances cytosolic ERK activity but inhibits ERK-mediated transcription following angiotensin AT1a receptor stimulation. 
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  Keywords of People

  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology