Immunoglobulin Variable Region

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  Subject Areas on Research

  • 125I-labeled anti-epidermal growth factor receptor-vIII single-chain Fv exhibits specific and high-level targeting of glioma xenografts. 
  • 98% IGHV gene identity is the optimal cutoff to dichotomize the prognosis of Chinese patients with chronic lymphocytic leukemia. 
  • A bidirectional phage display vector for the selection and mass transfer of polyclonal antibody libraries. 
  • A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion. 
  • A distinct wave of human T cell receptor gamma/delta lymphocytes in the early fetal thymus: evidence for controlled gene rearrangement and cytokine production. 
  • A method for linking VL and VH region genes that allows bulk transfer between vectors for use in generating polyclonal IgG libraries. 
  • A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia. 
  • Abundant tyrosine residues in the antigen binding site in anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: Application to radiolabeling. 
  • An entirely cell-based system to generate single-chain antibodies against cell surface receptors. 
  • Anti-DNA antibodies from autoimmune mice arise by clonal expansion and somatic mutation. 
  • Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region. 
  • Anti-laminin reactivity and glomerular immune deposition by in vitro recombinant antibodies. 
  • Antigen-binding repertoire and Ig H chain gene usage among B cell hybridomas from normal and autoimmune mice. 
  • Autoantibodies and their significance. 
  • Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance. 
  • B cells in epithelial and perivascular compartments of human adult thymus. 
  • CD28 is required for germinal center formation. 
  • Clinical and molecular predictors of disease severity and survival in chronic lymphocytic leukemia. 
  • Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling. 
  • Cloning of mitogen- and antigen-reactive B lymphocytes on filter paper discs. II. Paratope frequencies within the mitogen-selected repertoire. 
  • Construction of polyclonal antibody libraries using phage display. 
  • Contribution of the VH11 gene family to mitogen-responsive B cell repertoire in C57BL/6 mice. 
  • Developmental regulation of V(D)J recombination at the TCR alpha/delta locus. 
  • Differences in V kappa gene utilization and VH CDR3 sequence among anti-DNA from C3H-lpr mice and lupus mice with nephritis. 
  • Disparity in the kinetics of onset of hypermutation in immunoglobulin heavy and light chains. 
  • Enhanced HIV-1 neutralization by a CD4-VH3-IgG1 fusion protein. 
  • Evaluation of an anti-p185(HER2) (scFv-C(H)2-C(H)3)2 fragment following radioiodination using two different residualizing labels: SGMIB and IB-Mal-D-GEEEK. 
  • Facultative role of germinal centers and T cells in the somatic diversification of IgVH genes. 
  • GNA13 loss in germinal center B cells leads to impaired apoptosis and promotes lymphoma in vivo. 
  • Gene segment selection in V(D)J recombination: accessibility and beyond. 
  • Generation and characterization of a mouse/human chimeric antibody directed against extracellular matrix protein tenascin. 
  • Generation of a polyclonal Fab phage display library to the protozoan parasite Cryptosporidium parvum. 
  • Genome-wide high resolution DNA profiling of hairy cell leukaemia. 
  • Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice. 
  • Germline sequences of V(H)7183 gene family members in C57BL/6 mice demonstrate natural selection of particular sequences during recent evolution. 
  • HIV vaccine delayed boosting increases Env variable region 2-specific antibody effector functions. 
  • HIV-1 VACCINES. Diversion of HIV-1 vaccine-induced immunity by gp41-microbiota cross-reactive antibodies. 
  • HIV-1 gp120 vaccine induces affinity maturation in both new and persistent antibody clonal lineages. 
  • HIV-1 neutralization by chimeric CD4-CG10 polypeptides fused to human IgG1. 
  • Heavy-chain receptor editing unbound. 
  • Hepatitis B virus-associated diffuse large B-cell lymphoma: unique clinical features, poor outcome, and hepatitis B surface antigen-driven origin. 
  • Humoral immune responses in Cr2-/- mice: enhanced affinity maturation but impaired antibody persistence. 
  • IGHV1-69 B cell chronic lymphocytic leukemia antibodies cross-react with HIV-1 and hepatitis C virus antigens as well as intestinal commensal bacteria. 
  • Identification and utilization of arbitrary correlations in models of recombination signal sequences. 
  • Identification of recombinant antibodies against multiple distinct toll-like receptors by homolog mining a single immune scFv phage library. 
  • Idiotypic determinants used in the analysis of antibody diversification and as regulatory targets. 
  • Ig VH hypermutation is absent in the germinal centers of aged mice. 
  • Immunoglobulin gene hypermutation in germinal centers is independent of the RAG-1 V(D)J recombinase. 
  • Improved antigen binding by a CD20-specific single-chain antibody fragment with a mutation in CDRH1. 
  • In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. I. The architecture and dynamics of responding cell populations. 
  • In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. III. The kinetics of V region mutation and selection in germinal center B cells. 
  • Increased binding affinity enhances targeting of glioma xenografts by EGFRvIII-specific scFv. 
  • Independently derived murine glomerular immune deposit-forming anti-DNA antibodies are encoded by near-identical VH gene sequences. 
  • Intraclonal generation of antibody mutants in germinal centres. 
  • Isolation of a human anti-HIV gp41 membrane proximal region neutralizing antibody by antigen-specific single B cell sorting. 
  • Kappa editing rescues autoreactive B cells destined for deletion in mice transgenic for a dual specific anti-laminin Ig. 
  • Long-term outcomes for ibrutinib-rituximab and chemoimmunotherapy in CLL: updated results of the E1912 trial. 
  • Lymphocyte development and selection in germinal centers. 
  • Mechanisms of anti-DNA production in human and murine SLE. 
  • Molecular analysis of spontaneous nephrotropic anti-laminin antibodies in an autoimmune MRL-lpr/lpr mouse. 
  • Molecular and structural analysis of nuclear localizing anti-DNA lupus antibodies. 
  • Molecular characterization of anti-DNA antibodies induced in normal mice by immunization with bacterial DNA. Differences from spontaneous anti-DNA in the content and location of VH CDR3 arginines. 
  • Molecular composition of an antigen-specific, Ly-1 T suppressor inducer factor. One molecule binds antigen and is I-J-; another is I-J+, does not bind antigen, and imparts an Igh-variable region-linked restriction. 
  • Mouse/human chimeric Me1-14 antibody: genomic cloning of the variable region genes, linkage to human constant region genes, expression, and characterization. 
  • Multiple, conserved cryptic recombination signals in VH gene segments: detection of cleavage products only in pro B cells. 
  • Murine V kappa gene expression does not follow the VH paradigm. 
  • Network interactions, 1983. 
  • Oligoclonal TRBV gene usage among CD8(+) T cells in monoclonal B lymphocytosis and CLL. 
  • Pairing of VH gene families with the lambda 1 light chain: evidence for a non-stochastic association. 
  • Pairing of VK and VK gene families in self-reactive antibodies. 
  • Preconfiguration of the antigen-binding site during affinity maturation of a broadly neutralizing influenza virus antibody. 
  • Recombinant immunotoxin containing a disulfide-stabilized Fv directed at erbB2 that does not require proteolytic activation. 
  • Recombinant immunotoxins specific for a mutant epidermal growth factor receptor: targeting with a single chain antibody variable domain isolated by phage display. 
  • Recombinant polyclonal antibody libraries. 
  • Redemption of autoreactive B cells. 
  • Regional treatment of epidermal growth factor receptor vIII-expressing neoplastic meningitis with a single-chain immunotoxin, MR-1. 
  • Relaxed negative selection in germinal centers and impaired affinity maturation in bcl-xL transgenic mice. 
  • Rescue of HIV-1 broad neutralizing antibody-expressing B cells in 2F5 VH x VL knockin mice reveals multiple tolerance controls. 
  • Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women. 
  • SLE nephritis is associated with an oligoclonal expansion of intrarenal T cells. 
  • Single-cell analysis reveals oligoclonality among 'low-count' monoclonal B-cell lymphocytosis. 
  • Skewed VH and V kappa gene family expression and pairing occurs among B lymphocytes in autoimmune motheaten mice. 
  • Somatic diversification of antibody responses. 
  • Stochastic pairing of heavy-chain and kappa light-chain variable gene families occurs in polyclonally activated B cells. 
  • Structural features of nephritogenic lupus autoantibodies. 
  • Structural properties of a subset of nephritogenic anti-DNA antibodies. 
  • Structure and function of anti-DNA autoantibodies derived from a single autoimmune mouse. 
  • Studies of the humoral immune response. 
  • Targeted inhibition of V(D)J recombination by a histone methyltransferase. 
  • The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. 
  • The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors. 
  • The influence of variable-region somatic mutations on the specificity and pathogenicity of murine monoclonal anti-DNA antibodies. 
  • The mechanism of autoantibody production in an autoimmune MRL/lpr mouse. 
  • The nude mutation results in impaired primary antibody repertoire. 
  • The primary antibody repertoire: the somatic genotype and paratopic phenotype of B-cell populations. 
  • Two classes of germline genes both derived from the V(H)1 family direct the formation of human antibodies that recognize distinct antigenic sites in the C2 domain of factor VIII. 
  • Two distinct broadly neutralizing antibody specificities of different clonal lineages in a single HIV-1-infected donor: implications for vaccine design. 
  • V(D)J hypermutation and receptor revision: coloring outside the lines. 
  • V(D)J recombinase activity in a subset of germinal center B lymphocytes. 
  • VH gene analysis of spontaneously activated B cells in adult MRL-lpr/lpr mice. J558 bias is not limited to classic lupus autoantibodies. 
  • Variable region sequence analysis of anti-DNA antibodies: evidence for a family of closely related germ-line VH genes encoding lupus autoantibodies. 
  • Viral receptor-binding site antibodies with diverse germline origins. 
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  Keywords of People

  • Ferrari, Guido, Professor in Surgery, Molecular Genetics and Microbiology
  • Kelsoe, Garnett H., James B. Duke Distinguished Professor of Immunology, Integrative Immunobiology
  • Krangel, Michael S., George Barth Geller Distinguished Professor of Immunology, Integrative Immunobiology