Immunoglobulin Heavy Chains

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  Subject Areas on Research

  • A bidirectional phage display vector for the selection and mass transfer of polyclonal antibody libraries. 
  • A method for linking VL and VH region genes that allows bulk transfer between vectors for use in generating polyclonal IgG libraries. 
  • A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia. 
  • Analysis of somatic hypermutation in X-linked hyper-IgM syndrome shows specific deficiencies in mutational targeting. 
  • Anti-DNA antibodies from autoimmune mice arise by clonal expansion and somatic mutation. 
  • Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region. 
  • Anti-laminin reactivity and glomerular immune deposition by in vitro recombinant antibodies. 
  • Antibodies VRC01 and 10E8 neutralize HIV-1 with high breadth and potency even with Ig-framework regions substantially reverted to germline. 
  • Antigen-binding repertoire and Ig H chain gene usage among B cell hybridomas from normal and autoimmune mice. 
  • Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance. 
  • Binding of IgG to amyloid beta A4 peptide via the heavy-chain hinge region with preservation of antigen binding. 
  • Clinical and molecular predictors of disease severity and survival in chronic lymphocytic leukemia. 
  • Clonally related composite follicular lymphoma and mantle cell lymphoma with clinicopathologic features and biological implications. 
  • Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling. 
  • Complex regulation of somatic hypermutation by cis-acting sequences in the endogenous IgH gene in hybridoma cells. 
  • Composite mantle cell lymphoma and chronic lymphocytic leukemia/small lymphocytic lymphoma: a clinicopathologic and molecular study. 
  • Construction of polyclonal antibody libraries using phage display. 
  • Contribution of the VH11 gene family to mitogen-responsive B cell repertoire in C57BL/6 mice. 
  • Critical analysis and diagnostic usefulness of limited immunophenotyping of B-cell non-Hodgkin lymphomas by flow cytometry. 
  • Deconstructing B cell tolerance to basement membranes. 
  • Decreased frequency of somatic hypermutation and impaired affinity maturation but intact germinal center formation in mice expressing antisense RNA to DNA polymerase zeta. 
  • Delineation of immunologically and biochemically distinct HLA-A2 antigens. 
  • Detection of clonal lymphoid receptor gene rearrangements in langerhans cell histiocytosis. 
  • Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes. 
  • Disparity in the kinetics of onset of hypermutation in immunoglobulin heavy and light chains. 
  • Double IGHV DNA gene rearrangements in CLL: influence of mixed-mutated and -unmutated rearrangements on outcomes in CLL. 
  • Enhancement of anti-tumor immunity through local modulation of CTLA-4 and GITR by dendritic cells. 
  • Expression of activation-induced cytidine deaminase is regulated by cell division, providing a mechanistic basis for division-linked class switch recombination. 
  • Facultative role of germinal centers and T cells in the somatic diversification of IgVH genes. 
  • Flow cytometric analysis of immunoglobulin heavy chain expression in B-cell lymphoma and reactive lymphoid hyperplasia. 
  • Focused evolution of HIV-1 neutralizing antibodies revealed by structures and deep sequencing. 
  • Frequent detection of bcl-2/JH translocations in human blood and organ samples by a quantitative polymerase chain reaction assay. 
  • Functional and molecular characterization of human monoclonal antibody reactive with the immunodominant region of HIV type 1 glycoprotein 41. 
  • GNA13 loss in germinal center B cells leads to impaired apoptosis and promotes lymphoma in vivo. 
  • Gene profiling of canine B-cell lymphoma reveals germinal center and postgerminal center subtypes with different survival times, modeling human DLBCL. 
  • Generation of a polyclonal Fab phage display library to the protozoan parasite Cryptosporidium parvum. 
  • Genetic control of the immune response to staphylococcal nuclease. IX. Recombination between genes determining BALB/c antinuclease idiotypes and the heavy chain allotype locus. 
  • Genomic complexity identifies patients with aggressive chronic lymphocytic leukemia. 
  • Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice. 
  • Genotypic divergence precedes clinical dissemination in a case of synchronous bilateral B-cell malignant lymphoma of the testes. 
  • Germline sequences of V(H)7183 gene family members in C57BL/6 mice demonstrate natural selection of particular sequences during recent evolution. 
  • HIV-1 VACCINES. Diversion of HIV-1 vaccine-induced immunity by gp41-microbiota cross-reactive antibodies. 
  • HIV-1 gp140 epitope recognition is influenced by immunoglobulin DH gene segment sequence. 
  • Hairy Cell Leukemia, Version 2.2018, NCCN Clinical Practice Guidelines in Oncology. 
  • Heavy-chain receptor editing unbound. 
  • High frequency of clonal immunoglobulin receptor gene rearrangements in sporadic histiocytic/dendritic cell sarcomas. 
  • Histiocytic sarcoma arising in indolent small B-cell lymphoma: report of two cases with molecular/genetic evidence suggestive of a 'transdifferentiation' during the clonal evolution. 
  • Humoral autoimmunity to basement membrane antigens is regulated in C57BL/6 and MRL/MpJ mice transgenic for anti-laminin Ig receptors. 
  • IGHV1-69 B cell chronic lymphocytic leukemia antibodies cross-react with HIV-1 and hepatitis C virus antigens as well as intestinal commensal bacteria. 
  • Identification of an activity in B-cell extracts that selectively impairs the formation of an immunoglobulin mu s poly(A) site processing complex. 
  • Ig VH hypermutation is absent in the germinal centers of aged mice. 
  • Immunodominance of Antibody Recognition of the HIV Envelope V2 Region in Ig-Humanized Mice. 
  • Immunotoxins with increased activity against epidermal growth factor receptor vIII-expressing cells produced by antibody phage display. 
  • Improved antigen binding by a CD20-specific single-chain antibody fragment with a mutation in CDRH1. 
  • In situ studies of the germinal center reaction. 
  • In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. II. A common clonal origin for periarteriolar lymphoid sheath-associated foci and germinal centers. 
  • In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. III. The kinetics of V region mutation and selection in germinal center B cells. 
  • In vitro and in vivo expression of a nephritogenic Ig heavy chain determinant: pathogenic autoreactivity requires permissive light chains. 
  • In vivo functional analysis of in vitro protein binding sites in the immunoglobulin heavy chain enhancer. 
  • Independently derived murine glomerular immune deposit-forming anti-DNA antibodies are encoded by near-identical VH gene sequences. 
  • Induction of HIV Neutralizing Antibody Lineages in Mice with Diverse Precursor Repertoires. 
  • Influence of heavy chain constant regions on antigen binding and HIV-1 neutralization by a human monoclonal antibody. 
  • Inter-VH-gene-family shared idiotype on acquired immunodeficiency syndrome-associated lymphomas. 
  • Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation. 
  • Interdependence of N nucleotide addition and recombination site choice in V(D)J rearrangement. 
  • Kappa editing rescues autoreactive B cells destined for deletion in mice transgenic for a dual specific anti-laminin Ig. 
  • Long antibody HCDR3s from HIV-naïve donors presented on a PG9 neutralizing antibody background mediate HIV neutralization. 
  • Lupus-like nephrotropic autoantibodies in non-autoimmune mice harboring an anti-basement membrane/anti-DNA Ig heavy chain transgene. 
  • Metachronous/concomitant B-cell neoplasms with discordant light-chain or heavy-chain isotype restrictions: evidence of distinct B-cell neoplasms rather than clonal evolutions. 
  • Mining the antibodyome for HIV-1-neutralizing antibodies with next-generation sequencing and phylogenetic pairing of heavy/light chains. 
  • Molecular analysis of spontaneous nephrotropic anti-laminin antibodies in an autoimmune MRL-lpr/lpr mouse. 
  • Molecular characteristics of mantle cell lymphoma presenting with clonal plasma cell component. 
  • Molecular characterization of anti-DNA antibodies induced in normal mice by immunization with bacterial DNA. Differences from spontaneous anti-DNA in the content and location of VH CDR3 arginines. 
  • Molecular composition of an antigen-specific, Ly-1 T suppressor inducer factor. One molecule binds antigen and is I-J-; another is I-J+, does not bind antigen, and imparts an Igh-variable region-linked restriction. 
  • Mucosal vaccine targeting improves onset of mucosal and systemic immunity to botulinum neurotoxin A. 
  • Murine V kappa gene expression does not follow the VH paradigm. 
  • New presentation of alpha heavy chain disease: North American polypoid gastrointestinal lymphoma. Clinical and cellular studies. 
  • Pairing of VH gene families with the lambda 1 light chain: evidence for a non-stochastic association. 
  • Patients with chronic lymphocytic leukemia with high-risk genomic features have inferior outcome on successive Cancer and Leukemia Group B trials with alemtuzumab consolidation: subgroup analysis from CALGB 19901 and CALGB 10101. 
  • Patterns of heavy and light chain utilization in the antibody response to single-stranded bacterial DNA in normal human subjects and patients with systemic lupus erythematosus. 
  • Progressive immunoglobulin gene mutations in chronic lymphocytic leukemia: evidence for antigen-driven intraclonal diversification. 
  • Prominent Dutcher body formation in a case of follicular lymphoma with BCL6 gene rearrangement and intact BCL2 gene. 
  • Reduced thymic output, increased spontaneous apoptosis and oligoclonal B cells in polyethylene glycol-adenosine deaminase-treated patients. 
  • Regulation of basement membrane-reactive B cells in BXSB, (NZBxNZW)F1, NZB, and MRL/lpr lupus mice. 
  • Restricted isotype, distinct variable gene usage, and high rate of gp120 specificity of HIV-1 envelope-specific B cells in colostrum compared with those in blood of HIV-1-infected, lactating African women. 
  • Sensitization with xenogeneic tissues alters the heavy chain repertoire of human anti-Galalpha1-3Gal antibodies. 
  • Single-cell analysis reveals oligoclonality among 'low-count' monoclonal B-cell lymphocytosis. 
  • Skewed VH and V kappa gene family expression and pairing occurs among B lymphocytes in autoimmune motheaten mice. 
  • Stochastic pairing of heavy-chain and kappa light-chain variable gene families occurs in polyclonally activated B cells. 
  • Structural analysis of an HLA-B7 antigen variant detected by cytotoxic T lymphocytes. 
  • Structural properties of a subset of nephritogenic anti-DNA antibodies. 
  • The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. 
  • The endogenous immunoglobulin heavy chain enhancer can activate tandem VH promoters separated by a large distance. 
  • The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors. 
  • The nude mutation results in impaired primary antibody repertoire. 
  • The primary antibody repertoire: the somatic genotype and paratopic phenotype of B-cell populations. 
  • Topology of an amiloride-binding protein. 
  • Transcriptional enhancer elements in the mouse immunoglobulin heavy chain locus. 
  • Two classes of germline genes both derived from the V(H)1 family direct the formation of human antibodies that recognize distinct antigenic sites in the C2 domain of factor VIII. 
  • VH gene analysis of spontaneously activated B cells in adult MRL-lpr/lpr mice. J558 bias is not limited to classic lupus autoantibodies. 
  • Very low affinity B cells form germinal centers, become memory B cells, and participate in secondary immune responses when higher affinity competition is reduced. 
  • Viral receptor-binding site antibodies with diverse germline origins. 
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  Keywords of People

  • Kelsoe, Garnett H., James B. Duke Professor of Immunology, Immunology