Immunosorbent Techniques

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  Subject Areas on Research

  • A new erythrocyte membrane-associated protein with calmodulin binding activity. Identification and purification. 
  • A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assays. 
  • AQP2 is a substrate for endogenous PP2B activity within an inner medullary AKAP-signaling complex. 
  • Activation of mitogen-activated protein kinase kinase by v-Raf in NIH 3T3 cells and in vitro. 
  • Anti-receptor antibody-induced H-Y-specific delayed-type hypersensitivity responses in nonresponder mice. 
  • Colocalization of band 3 with ankyrin and spectrin at the basal membrane of intercalated cells in the rat kidney. 
  • Contact-inhibition-induced quiescent state is marked by intense nuclear expression of statin. 
  • Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2. 
  • Evidence against posttranslational glycosylation of rat glucocorticoid receptors. 
  • Evidence for polyreactive xenoreactive antibodies in the repertoire of human anti-swine antibodies: the 'next' humoral barrier to xenotransplantation? 
  • Evidence for preferential adhesion of ovarian epithelial carcinoma cells to type I collagen mediated by the alpha2beta1 integrin. 
  • Evidence of juxtacrine signaling for transforming growth factor alpha in human endometrium. 
  • Expression and sequence analysis of a cDNA encoding the orotidine-5'-monophosphate decarboxylase domain from Ehrlich ascites uridylate synthase. 
  • Hypothermic reperfusion after cardiac arrest augments brain-derived neurotrophic factor activation. 
  • Identification and partial purification of ankyrin, the high affinity membrane attachment site for human erythrocyte spectrin. 
  • Identification of human erythrocyte blood group antigens on decay-accelerating factor (DAF) and an erythrocyte phenotype negative for DAF. 
  • IgA immune complexes in patients with dermatitis herpetiformis occur in the absence of IgA rheumatoid factor. 
  • Immunodiagnosis of infection with Schistosoma mansoni: enzyme-linked immunosorbent assay for detection of antibody to circulating antigen. 
  • In vitro synthesis of laminin and entactin polypeptides. 
  • Isoform-specific retinoid-X receptor (RXR) antibodies detect differential expression of RXR proteins in the pituitary gland. 
  • Isolation of an ankyrin-band 3 oligomer from human erythrocyte membranes. 
  • Isolation of unique STAT5 targets by chromatin immunoprecipitation-based gene identification. 
  • Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays. 
  • Non-anti-Gal alpha1-3Gal antibody mechanisms are sufficient to cause hyperacute lung dysfunction in pulmonary xenotransplantation. 
  • Normal cellular and transformation-associated abl proteins share common sites for protein kinase C phosphorylation. 
  • Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. 
  • Phosphorylation of the B1 (CD20) molecule by normal and malignant human B lymphocytes. 
  • Production of polyclonal and monoclonal antibodies to calmodulin and utilization of these immunological probes. 
  • Relationship between binding affinity, retention and sensitivity of human rhabdomyosarcoma xenografts to Vinca alkaloids. 
  • Restricted neutralization of divergent human T-lymphotropic virus type III isolates by antibodies to the major envelope glycoprotein. 
  • Selective externalization of an ATP-binding protein structurally related to the clathrin-uncoating ATPase/heat shock protein in vesicles containing terminal transferrin receptors during reticulocyte maturation. 
  • Specificity and distribution of the determinants of the major glycoprotein (gp71) of murine oncornaviruses as detected by an in vitro cytotoxicity assay. 
  • Synapsin I is a spectrin-binding protein immunologically related to erythrocyte protein 4.1. 
  • Synthesis of alpha 2-macroglobulin by bovine adrenal cortical cell cultures. 
  • The adaptor protein beta-arrestin2 enhances endocytosis of the low density lipoprotein receptor. 
  • The membrane attachment protein for spectrin is associated with band 3 in human erythrocyte membranes. 
  • The role of antibodies in acute vascular rejection of pig-to-baboon cardiac transplants. 
  • Transplantation in miniature swine. I. Fixation of the major histocompatibility complex. 
  • Two forms of the T-cell receptor gamma protein found on peripheral blood cytotoxic T lymphocytes. 
  • U1-snRNP-A protein selects a ten nucleotide consensus sequence from a degenerate RNA pool presented in various structural contexts. 
  • Utility of peritransplant and rescue intravenous immunoglobulin and extracorporeal immunoadsorption in lung transplant recipients sensitized to HLA antigens. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute