Isoelectric Focusing

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  Subject Areas on Research

  • A comparison of the constituent polypeptides of the B-800--850 light-harvesting pigment-protein complex from Rhodopseudomonas sphaeroides. 
  • Altered conformation of Gc (vitamin D-binding protein) upon complexing with cellular actin. 
  • Analysis of human serum proteins by liquid phase isoelectric focusing and matrix-assisted laser desorption/ionization-mass spectrometry. 
  • Antibody inhibitors of nonmuscle myosin function and assembly. 
  • Basal and stimulated protein S-nitrosylation in multiple cell types and tissues. 
  • Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes. 
  • Characterization of the acyl-CoA:amino acid N-acyltransferases from primate liver mitochondria. 
  • Comparison of the intestinal and serum antibody response in patients with dermatitis herpetiformis. 
  • Control of RhoA methylation by carboxylesterase I. 
  • Delineation of immunologically and biochemically distinct HLA-A2 antigens. 
  • Detection of CTX-M-15 and CTX-M-33, a novel variant of CTX-M-15, in clinical Escherichia coli isolates in Greece. 
  • Determination of glycosylated hemoglobin by affinity chromatography: comparison with colorimetric and ion-exchange methods, and effects of common interferences. 
  • Differential extraction and enrichment of human sperm surface proteins in a proteome: identification of immunocontraceptive candidates. 
  • Distinct sites on the G-actin molecule bind group-specific component and deoxyribonuclease I. 
  • Functional properties of the hemoglobin from the South American snake Mastigodryas bifossatus. 
  • Human B cell alloantigens; alpha subunit variability. 
  • Identification of glial filament protein and vimentin in the same intermediate filament system in human glioma cells. 
  • Increased expression of the glucose-regulated gene encoding the Mr 78,000 glucose-regulated protein in chemically and radiation-transformed C3H 10T1/2 mouse embryo cells. 
  • Isoelectric focusing in polyacrylamide-agarose. 
  • Isolation of the Maclura pomifera hemagglutinin on a deoxymelibiotol affinity support and preliminary characterization by buffer electrofocusing and high-performance liquid chromatography. 
  • Life with too much polyprenol: polyprenol reductase deficiency. 
  • Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath. 
  • Persistent alterations of calmodulin kinase II activity in chickens after an oral dose of tri-o-cresyl phosphate. 
  • Pitfalls in newborn hemoglobinopathy screening: failure to detect beta(+)-thalassemia. 
  • Preparation and characterization of lymphocyte-activating factor (LAF) from acute monocytic and myelomonocytic leukemia cells. 
  • Preparation of high molecular weight invertebrate hemoglobins. 
  • Preparative isoelectric focusing and preparative electrophoresis of hydrophobic Candida albicans cell wall proteins with in-line transfer to polyvinylidene difluoride membranes for sequencing. 
  • Production and characterization of the monoclonal antibody against SGP120, a novel serum protein. 
  • Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. 
  • Protein patterns of early mouse embryos during development. 
  • Purification and biochemical characterization of statin, a nonproliferation specific protein from rat liver. 
  • Structural analysis of an HLA-B7 antigen variant detected by cytotoxic T lymphocytes. 
  • Structure and oxygen equilibrium of the three coelomic cell hemoglobins of the echiuran worm Thalassema mellita (Conn). 
  • Subunit composition of high molecular weight oligomer: Limulus polyphemus hemocyanin. 
  • Terminin (Tp 63/60), a novel cell senescence-related protein, is present in the aging human hippocampus. 
  • The beta 1-adrenergic receptor of the turkey erythrocyte. Molecular heterogeneity revealed by purification and photoaffinity labeling. 
  • The membrane attachment protein for spectrin is associated with band 3 in human erythrocyte membranes. 
  • The release of acid phosphatase and polysaccharide- and protein-containing components from the surface of the dimorphic forms of Candida albicans by treatment with dithiothreitol. 
  • The subunit structure of normal and hemophilic factor VIII. 
  • Thermodynamics of the hydrolysis of penicillin G and ampicillin. 
  • Trimethoprim resistance in Haemophilus influenzae is due to altered dihydrofolate reductase(s). 
  • Virus-immune cytotoxic T cells recognize structural differences between serologically indistinguishable HLA-A2 molecules. 
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  Keywords of People

  • Bennett, Vann, George B. Geller Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute