Isotope Labeling

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  Subject Areas on Research

  • 13C NMR isotopomer analysis reveals a connection between pyruvate cycling and glucose-stimulated insulin secretion (GSIS). 
  • 211At- and 131I-labeled bisphosphonates with high in vivo stability and bone accumulation. 
  • 5-substituted derivatives of 6-halogeno-3-((2-(S)-azetidinyl)methoxy)pyridine and 6-halogeno-3-((2-(S)-pyrrolidinyl)methoxy)pyridine with low picomolar affinity for alpha4beta2 nicotinic acetylcholine receptor and wide range of lipophilicity: potential probes for imaging with positron emission tomography. 
  • A kit method for the high level synthesis of [211At]MABG. 
  • A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine. 
  • A procedure for the preferential radioiodination of small amounts of protein in the presence of excess lipids. 
  • A roadmap for interpreting (13)C metabolite labeling patterns from cells. 
  • A thermally responsive biopolymer for intra-articular drug delivery 
  • An alternative and expedient synthesis of radioiodinated 4-iodophenylalanine. 
  • Analysis of oxygen/glucose-deprivation-induced changes in SUMO3 conjugation using SILAC-based quantitative proteomics. 
  • Antiepidermal growth factor variant III scFv fragment: effect of radioiodination method on tumor targeting and normal tissue clearance. 
  • Astatination of proteins using an N-succinimidyl tri-n-butylstannyl benzoate intermediate. 
  • Astatine-211 labeling of internalizing anti-EGFRvIII monoclonal antibody using N-succinimidyl 5-[211At]astato-3-pyridinecarboxylate. 
  • Autodegradation of 125I-labeled human epidermal cell surface proteins. 
  • Automated radiosynthesis of [11C]morphine for clinical investigation. 
  • Brush border enzyme-cleavable linkers: Evaluation for reducing renal uptake of radiolabeled prostate-specific membrane antigen inhibitors. 
  • Capillary Electrophoresis-High Resolution Mass Spectrometry for Measuring In Vivo Arginine Isotope Incorporation in Alzheimer's Disease Mouse Models. 
  • Catabolism of radioiodinated murine monoclonal antibody F(ab')2 fragment labeled using N-succinimidyl 3-iodobenzoate and Iodogen methods. 
  • Characterization of symbiont populations in life-history stages of mussels from chemosynthetic environments. 
  • Characterization of the Saccharomyces cerevisiae ATP-Interactome using the iTRAQ-SPROX Technique. 
  • Chimeric anti-tenascin antibody 81C6: increased tumor localization compared with its murine parent. 
  • Clinical experience with alpha-particle emitting 211At: treatment of recurrent brain tumor patients with 211At-labeled chimeric antitenascin monoclonal antibody 81C6. 
  • Computerized liquid scintillation counting data management. 1. Time sequence for decay correction: a key for data storage and retrieval. 
  • Constrained selected reaction monitoring: quantification of selected post-translational modifications and protein isoforms. 
  • Current trends in differential expression proteomics: isotopically coded tags. 
  • Cytotoxicity of alpha-particle-emitting 5-[211At]astato-2'-deoxyuridine in human cancer cells. 
  • D-Amino acid peptide residualizing agents bearing N-hydroxysuccinimido- and maleimido-functional groups and their application for trastuzumab radioiodination. 
  • Detection of oncornavirus antigenic activity in human urothelial tissues. 
  • Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC). 
  • Development of a radioimmunoassay for fetal antigen 2. 
  • Discovery of Manassantin A Protein Targets Using Large-Scale Protein Folding and Stability Measurements. 
  • Effect of radioiodination on the binding of monoclonal antibody DF3 to breast carcinoma cells. 
  • Enhanced binding and inertness to dehalogenation of alpha-melanotropic peptides labeled using N-succinimidyl 3-iodobenzoate. 
  • Evaluation of a simpler technique for in-vivo labelling of red blood cells with 99mTc. 
  • Evaluation of an internalizing monoclonal antibody labeled using N-succinimidyl 3-[131I]iodo-4-phosphonomethylbenzoate ([131I]SIPMB), a negatively charged substituent bearing acylation agent. 
  • Fluorine-18 labeling of monoclonal antibodies and fragments with preservation of immunoreactivity. 
  • Fluorine-18-labeled monoclonal antibody fragments: a potential approach for combining radioimmunoscintigraphy and positron emission tomography. 
  • Global analysis of protein folding thermodynamics for disease state characterization. 
  • High-level production of alpha-particle-emitting (211)At and preparation of (211)At-labeled antibodies for clinical use. 
  • Hyperthermia increases accumulation of technetium-99m-labeled liposomes in feline sarcomas. 
  • Identification of Protease Specificity by Combining Proteome-Derived Peptide Libraries and Quantitative Proteomics. 
  • Identification of S-nitrosylated targets of thioredoxin using a quantitative proteomic approach. 
  • Identification of dengue RNA binding proteins using RNA chromatography and quantitative mass spectrometry. 
  • Improved synthesis of N-succinimidyl 4-[18F]fluorobenzoate and its application to the labeling of a monoclonal antibody fragment. 
  • Improved tumor targeting of anti-HER2 nanobody through N-succinimidyl 4-guanidinomethyl-3-iodobenzoate radiolabeling. 
  • Inositol phosphate metabolomics: merging genetic perturbation with modernized radiolabeling methods. 
  • Labeling Monoclonal Antibody with α-emitting 211At at High Activity Levels via a Tin Precursor. 
  • Labeling internalizing anti-epidermal growth factor receptor variant III monoclonal antibody with (177)Lu: in vitro comparison of acyclic and macrocyclic ligands. 
  • Labeling monoclonal antibodies with halogen nuclides. 
  • Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability. 
  • Labeling proteins with fluorine-18 using N-succinimidyl 4-[18F]fluorobenzoate. 
  • Large-Scale Analysis of Breast Cancer-Related Conformational Changes in Proteins Using SILAC-SPROX. 
  • Matching isotopic distributions from metabolically labeled samples. 
  • Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS. 
  • Measuring long-lived 13C2 state lifetimes at natural abundance. 
  • Method for radioiodination of proteins using N-succinimidyl 3-hydroxy-4-iodobenzoate. 
  • Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in Mammalian cells. 
  • Monitoring protein conformational changes and dynamics using stable-isotope labeling and mass spectrometry. 
  • N-Succinimidyl guanidinomethyl iodobenzoate protein radiohalogenation agents: influence of isomeric substitution on radiolabeling and target cell residualization. 
  • N-succinimidyl 3-[(131)I]iodo-4-phosphonomethylbenzoate ([(131)I]SIPMB), a negatively charged substituent-bearing acylation agent for the radioiodination of peptides and mAbs. 
  • N-succinimidyl 4-methyl-3-(tri-n-butylstannyl)benzoate: synthesis and potential utility for the radioiodination of monoclonal antibodies. 
  • No-carrier-added (4-fluoro-3-[131I]iodobenzyl)guanidine and (3-[211At]astato-4-fluorobenzyl)guanidine. 
  • No-carrier-added synthesis of a 4-methyl-substituted meta-iodobenzylguanidine analogue. 
  • No-carrier-added synthesis of meta-[131I]iodobenzylguanidine. 
  • Optical and radioiodinated tethered Hsp90 inhibitors reveal selective internalization of ectopic Hsp90 in malignant breast tumor cells. 
  • Organ distribution of 99mTc-labelled aminethiols. 
  • Overview of protein phosphorylation. 
  • Photoaffinity cross-linking of a radioiodinated probe, 125I-A55453, into alpha 1-adrenergic receptors. 
  • Positively charged templates for labeling internalizing antibodies: comparison of N-succinimidyl 5-iodo-3-pyridinecarboxylate and the D-amino acid peptide KRYRR. 
  • Positron emission tomography for the evaluation of pancreatic disease. 
  • Preclinical Evaluation of 18F-Labeled Anti-HER2 Nanobody Conjugates for Imaging HER2 Receptor Expression by Immuno-PET. 
  • Preparation of 5-[131I]iodo- and 5-[211At]astato-1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl) uracil by a halodestannylation reaction. 
  • Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins. 
  • Preparation of a biologically stable and immunogenically competent astatinated protein. 
  • Proteome analysis by mass spectrometry. 
  • Proteome-Wide Characterization of Phosphorylation-Induced Conformational Changes in Breast Cancer. 
  • Proteome-Wide Structural Biology: An Emerging Field for the Structural Analysis of Proteins on the Proteomic Scale. 
  • Proteomic analysis of proteins related to rice grain chalkiness using iTRAQ and a novel comparison system based on a notched-belly mutant with white-belly. 
  • Proteomic characterization of the cellular response to nitrosative stress mediated by s-nitrosoglutathione reductase inhibition. 
  • Proteomic identification and functional characterization of MYH9, Hsc70, and DNAJA1 as novel substrates of HDAC6 deacetylase activity. 
  • Proteomic profiling of a layered tissue reveals unique glycolytic specializations of photoreceptor cells. 
  • Proteomics in prostate cancer. 
  • Radiohalogenation of a monoclonal antibody using an N-succinimidyl 3-(tri-n-butylstannyl)benzoate intermediate. 
  • Radioimmunotherapy of breast cancer xenografts with monoclonal antibody ICR12 against c-erbB2 p185: comparison of iodogen and N-succinimidyl 4-methyl-3-(tri-n-butylstannyl)benzoate radioiodination methods. 
  • Radioiodinated antibody targeting of the HER-2/neu oncoprotein: effects of labeling method on cellular processing and tissue distribution. 
  • Radioiodination and astatination of octreotide by conjugation labeling. 
  • Radioiodination of a monoclonal antibody using N-succinimidyl 5-iodo-3-pyridinecarboxylate. 
  • Radioiodination of antibodies via N-succinimidyl 2,4-dimethoxy-3-(trialkylstannyl)benzoates. 
  • Radioiodination of internalizing monoclonal antibodies using N-succinimidyl 5-iodo-3-pyridinecarboxylate. 
  • Radioiodination of proteins using N-succinimidyl 4-hydroxy-3-iodobenzoate. 
  • Radioiodination via D-amino acid peptide enhances cellular retention and tumor xenograft targeting of an internalizing anti-epidermal growth factor receptor variant III monoclonal antibody. 
  • Radiolabeled inhibitors as probes for imaging mutant IDH1 expression in gliomas: Synthesis and preliminary evaluation of labeled butyl-phenyl sulfonamide analogs. 
  • Radiolabeling and in vitro evaluation of (67)Ga-NOTA-modular nanotransporter--a potential Auger electron emitting EGFR-targeted radiotherapeutic. 
  • Radiolabelling of glucose-Tyr3-octreotate with 125I and analysis of its metabolism in rats: comparison with radiolabelled DOTA-Tyr3-octreotate. 
  • Radiopharmaceutical chemistry of targeted radiotherapeutics, Part 2: radiolytic effects of 211At alpha-particles influence N-succinimidyl 3-211AT-astatobenzoate synthesis. 
  • Radiopharmaceutical chemistry of targeted radiotherapeutics, part 4: Strategies for 211At labeling at high activities and radiation doses of 211At α-particles. 
  • Reagents for astatination of biomolecules: comparison of the in vivo distribution and stability of some radioiodinated/astatinated benzamidyl and nido-carboranyl compounds. 
  • Renal uptake of lutropin. Studies based on electron microscopic autoradiography and nephrectomy. 
  • Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis. 
  • SIB-DOTA: a trifunctional prosthetic group potentially amenable for multi-modal labeling that enhances tumor uptake of internalizing monoclonal antibodies. 
  • SILAC-pulse proteolysis: A mass spectrometry-based method for discovery and cross-validation in proteome-wide studies of ligand binding. 
  • Site of Tc-99m binding to the red blood cell: concise communication. 
  • Stable isotope labeling strategy for protein-ligand binding analysis in multi-component protein mixtures. 
  • StableIsotope Labeling with Amino Acids in Cell Culture (SILAC)-based strategy for proteome-wide thermodynamic analysis of protein-ligand binding interactions. 
  • Static secondary-ion mass spectrometric investigation of the surface structure of organic plasma-deposited films prepared from stable-isotope-labeled precursors. 1. Carbonyl precursors. 
  • Synthesis and evaluation of 18F-labeled choline as an oncologic tracer for positron emission tomography: initial findings in prostate cancer. 
  • Synthesis and evaluation of 4-[18F]fluoropropoxy-3-iodobenzylguanidine ([18F]FPOIBG): A novel 18F-labeled analogue of MIBG. 
  • Synthesis of 11C-labeled chlorpromazine directly from [11C]carbon dioxide. 
  • Synthesis of iodine-125 labeled (+/-)-15-(4-azidobenzyl)carazolol: a potent beta-adrenergic photoaffinity probe. 
  • Synthesis of radioiodinated N-succinimidyl iodobenzoate: optimization for use in antibody labelling. 
  • Synthesis of the labeled D1 receptor antagonist SCH 23390 using [11C]carbon dioxide. 
  • Synthesis, purification, and in vitro stability of 211At- and 125I-labeled amidobisphosphonates. 
  • The doubly labeled water method produces highly reproducible longitudinal results in nutrition studies. 
  • The sources of rat biliary cholesterol and bile acid. 
  • Tracking the in vivo fate of recombinant polypeptides by isotopic labeling 
  • Transfer of radioiodine from iodinated oncornavirus proteins to unlabeled protein carrier during routine procedures for peptide mapping. 
  • Treatment of HER2-positive breast carcinomatous meningitis with intrathecal administration of alpha-particle-emitting (211)At-labeled trastuzumab. 
  • Tumor blood flow measured by perfusion computed tomography and 15O-labeled water positron emission tomography: a comparison study. 
  • Tumor-specific anti-epidermal growth factor receptor variant III monoclonal antibodies: use of the tyramine-cellobiose radioiodination method enhances cellular retention and uptake in tumor xenografts. 
  • Understanding metabolism with flux analysis: From theory to application. 
  • Using dynamic programming to create isotopic distribution maps from mass spectra. 
  • Value and limitations of testicular scanning by in vivo labelling of RBC with 99mTc. 
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  Keywords of People

  • Abbruzzese, James, D. C. I. Distinguished Professor of Medical Oncology, Medicine, Medical Oncology
  • Chitneni, Satish K., Associate Professor in Radiology, Radiology
  • Farsiu, Sina, Professor in the Department of Biomedical Engineering, Electrical and Computer Engineering
  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Biomedical Engineering