Luciferases

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  Subject Areas on Research

  • A 40-kilodalton protein binds specifically to an upstream sequence element essential for muscle-specific transcription of the human myoglobin promoter. 
  • A CRISPR Activation Screen Identifies a Pan-avian Influenza Virus Inhibitory Host Factor. 
  • A conserved Pbx-Wnt-p63-Irf6 regulatory module controls face morphogenesis by promoting epithelial apoptosis. 
  • A critical role for CRM1 in regulating HOXA gene transcription in CALM-AF10 leukemias. 
  • A family of hierarchically self-assembling linear-dendritic hybrid polymers for highly efficient targeted gene delivery. 
  • A functional variant (-1304T>G) in the MKK4 promoter contributes to a decreased risk of lung cancer by increasing the promoter activity. 
  • A functional variant at the miR-184 binding site in TNFAIP2 and risk of squamous cell carcinoma of the head and neck. 
  • A novel bioluminescence orthotopic mouse model for advanced lung cancer. 
  • A physical interaction network of dengue virus and human proteins. 
  • A pressure-mediated nonviral method for efficient arterial gene and oligonucleotide transfer. 
  • A small-molecule antagonist of the hedgehog signaling pathway. 
  • Activated cAMP-response element-binding protein regulates neuronal expression of presenilin-1. 
  • Activator protein-1 has an essential role in pancreatic cancer cells and is regulated by a novel Akt-mediated mechanism. 
  • Activity of a type 1 picornavirus internal ribosomal entry site is determined by sequences within the 3' nontranslated region. 
  • Adeno-associated viral vectors based on serotype 3b use components of the fibroblast growth factor receptor signaling complex for efficient transduction. 
  • Alpha V integrin prolongs collagenase production through Jun activation binding protein 1. 
  • An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots. 
  • An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa. 
  • An oligonucleotide decoy for transcription factor E2F inhibits mesangial cell proliferation in vitro. 
  • Androgen receptor down-regulation in prostate cancer with phosphorodiamidate morpholino antisense oligomers. 
  • Aryl hydrocarbon receptor activation in genital tubercle, palate, and other embryonic tissues in 2,3,7, 8-tetrachlorodibenzo-p-dioxin-responsive lacZ mice. 
  • Association of a functional tandem repeats in the downstream of human telomerase gene and lung cancer. 
  • Ataxia-telangiectasia group D complementing gene (ATDC) promotes lung cancer cell proliferation by activating NF-κB pathway. 
  • Autoregulatory control of E2F1 expression in response to positive and negative regulators of cell cycle progression. 
  • Bioluminescent virion shells: new tools for quantitation of AAV vector dynamics in cells and live animals. 
  • CNS gene transfer mediated by a novel controlled release system based on DNA complexes of degradable polycation PPE-EA: a comparison with polyethylenimine/DNA complexes. 
  • Caspase 3 promotes surviving melanoma tumor cell growth after cytotoxic therapy. 
  • Cellular arrays for large-scale analysis of transcription factor activity. 
  • Clara cell secretory protein decreases lung inflammation after acute virus infection. 
  • Cloning and characterization of the major promoter of the human protein kinase C beta gene. Regulation by phorbol esters. 
  • Co-transactivation of the 3' erythropoietin hypoxia inducible enhancer by the HIF-1 protein. 
  • Coacervate microspheres as carriers of recombinant adenoviruses. 
  • Collaborative interactions between MEF-2 and Sp1 in muscle-specific gene regulation. 
  • Comparative analysis of novel and conventional Hsp90 inhibitors on HIF activity and angiogenic potential in clear cell renal cell carcinoma: implications for clinical evaluation. 
  • Competing Pathways and Multiple Folding Nuclei in a Large Multidomain Protein, Luciferase. 
  • Competitive translation efficiency at the picornavirus type 1 internal ribosome entry site facilitated by viral cis and trans factors. 
  • Conditional gene silencing in mammalian cells mediated by a stress-inducible promoter. 
  • Context-specific effects of fibulin-5 (DANCE/EVEC) on cell proliferation, motility, and invasion. Fibulin-5 is induced by transforming growth factor-beta and affects protein kinase cascades. 
  • Controlled gene delivery by DNA-gelatin nanospheres. 
  • Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3. 
  • Crystal structure of the human LRH-1 DBD-DNA complex reveals Ftz-F1 domain positioning is required for receptor activity. 
  • Cyclic AMP mediated GSTP1 gene activation in tumor cells involves the interaction of activated CREB-1 with the GSTP1 CRE: a novel mechanism of cellular GSTP1 gene regulation. 
  • Cyclin D1 inhibits peroxisome proliferator-activated receptor gamma-mediated adipogenesis through histone deacetylase recruitment. 
  • Derivation and characterization of a simian immunodeficiency virus SIVmac239 variant with tropism for CXCR4. 
  • Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs. 
  • Design, expression, and immunogenicity of a soluble HIV trimeric envelope fragment adopting a prefusion gp41 configuration. 
  • Determination of neutralization activities by a new versatile assay using an HIV-1 genome carrying the Gaussia luciferase gene. 
  • Development of a novel screen for protease inhibitors. 
  • Differential CD4/CCR5 utilization, gp120 conformation, and neutralization sensitivity between envelopes from a microglia-adapted human immunodeficiency virus type 1 and its parental isolate. 
  • Differential expression of intracellular and secreted osteopontin isoforms by murine macrophages in response to toll-like receptor agonists. 
  • Differential osteopontin expression in phenotypically distinct subclones of murine breast cancer cells mediates metastatic behavior. 
  • Dioxin induces transcription of fos and jun genes by Ah receptor-dependent and -independent pathways. 
  • Disruption of the basal body compromises proteasomal function and perturbs intracellular Wnt response. 
  • Divergent pathways mediate the induction of ANF transgenes in neonatal and hypertrophic ventricular myocardium. 
  • Effect of allelic variation at the NACP-Rep1 repeat upstream of the alpha-synuclein gene (SNCA) on transcription in a cell culture luciferase reporter system. 
  • Effect of side-chain structures on gene transfer efficiency of biodegradable cationic polyphosphoesters. 
  • Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery. 
  • Efficient in vivo gene transfer into the heart in the rat myocardial infarction model using the HVJ (Hemagglutinating Virus of Japan)--liposome method. 
  • Enhancement of rAAV2-mediated transgene expression in retina cells in vitro and in vivo by coadministration of low-dose chemotherapeutic drugs. 
  • Epidermal growth factor receptor trans-activation mediates the tonic and fibrogenic effects of endothelin in the aortic wall of transgenic mice. 
  • Epstein-Barr virus latent membrane protein 1 activation of NF-kappaB through IRAK1 and TRAF6. 
  • Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cells. 
  • Evaluating neutralizing antibodies against HIV, SIV, and SHIV in luciferase reporter gene assays. 
  • Evaluation of collagen and methylated collagen as gene carriers. 
  • Ex vivo adenovirus-mediated gene transfer to the adult rat heart. 
  • Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. 
  • Expression of CXC chemokine receptor-4 enhances the pulmonary metastatic potential of murine B16 melanoma cells. 
  • Formulations which increase the size of lipoplexes prevent serum-associated inhibition of transfection. 
  • Functional analysis of intra-allelic variation at NACP-Rep1 in the alpha-synuclein gene. 
  • Gamma-globin gene promoter elements required for interaction with globin enhancers. 
  • Genetic and functional dissection of HTRA1 and LOC387715 in age-related macular degeneration. 
  • Genomic distribution of CHD7 on chromatin tracks H3K4 methylation patterns. 
  • Genomic structure and embryonic expression of estrogen receptor beta a (ERbetaa) in zebrafish (Danio rerio). 
  • Heparan sulfate, an endogenous TLR4 agonist, promotes acute GVHD after allogeneic stem cell transplantation. 
  • High intensity focused ultrasound-induced gene activation in solid tumors. 
  • How petals change their spots: cis-regulatory re-wiring in Clarkia (Onagraceae). 
  • Human T-cell leukemia virus type 1 Tax activates cyclin-dependent kinase inhibitor p21/Waf1/Cip1 expression through a p53-independent mechanism: Inhibition of cdk2. 
  • Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing. 
  • Human olfactory receptor responses to odorants. 
  • Human progesterone receptor A form is a cell- and promoter-specific repressor of human progesterone receptor B function. 
  • Human retroviral gag- and gag-pol-like proteins interact with the transforming growth factor-beta receptor activin receptor-like kinase 1. 
  • Human topoisomerase I promotes HIV-1 proviral DNA synthesis: implications for the species specificity and cellular tropism of HIV-1 infection. 
  • Hypermethylation of growth arrest DNA damage-inducible gene 45 beta promoter in human hepatocellular carcinoma. 
  • Hypoxia-regulated therapeutic gene as a preemptive treatment strategy against ischemia/reperfusion tissue injury. 
  • Identification of an enhancer element in the estrogen receptor upstream region: implications for regulation of ER transcription in breast cancer. 
  • Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1. 
  • Imaging of energy metabolites (ATP, glucose and lactate) in tissue sections: a bioluminescent technique. 
  • In vivo bioluminescent imaging of influenza a virus infection and characterization of novel cross-protective monoclonal antibodies. 
  • In vivo regulation of hTERT expression and telomerase activity by androgen. 
  • Inhibition of constitutive NF-kappa B activity by I kappa B alpha M suppresses tumorigenesis. 
  • Inhibition of transforming growth factor-beta/SMAD signalling by the interferon-gamma/STAT pathway. 
  • Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration. 
  • Interaction of the Dr1 inhibitory factor with the TATA binding protein is disrupted by adenovirus E1A. 
  • Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. 
  • Involvement of the mt1 melatonin receptor in human breast cancer. 
  • Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor. 
  • Ligand-specific dynamics of the androgen receptor at its response element in living cells. 
  • Light-emitting channelrhodopsins for combined optogenetic and chemical-genetic control of neurons. 
  • Loss of type III transforming growth factor-beta receptor expression is due to methylation silencing of the transcription factor GATA3 in renal cell carcinoma. 
  • Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney. 
  • Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation. 
  • Maspin expression profile in human prostate cancer (CaP) and in vitro induction of Maspin expression by androgen ablation. 
  • MeCP2 reinforces STAT3 signaling and the generation of effector CD4+ T cells by promoting miR-124-mediated suppression of SOCS5. 
  • Measuring HIV neutralization in a luciferase reporter gene assay. 
  • Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy. 
  • Methylation of TFPI-2 gene is not the sole cause of its silencing. 
  • MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. 
  • Minimal and inducible regulation of tissue factor pathway inhibitor-2 in human gliomas. 
  • Molecular recognition of ketamine by a subset of olfactory G protein-coupled receptors. 
  • Multiple independent variants at the TERT locus are associated with telomere length and risks of breast and ovarian cancer. 
  • Myosin light chain-2 luciferase transgenic mice reveal distinct regulatory programs for cardiac and skeletal muscle-specific expression of a single contractile protein gene. 
  • NF-kappaB is essential for induction of CYLD, the negative regulator of NF-kappaB: evidence for a novel inducible autoregulatory feedback pathway. 
  • Naked DNA delivered intraportally expresses efficiently in hepatocytes. 
  • Newspapers and newspaper ink contain agonists for the ah receptor. 
  • Noninvasive imaging and quantification of epidermal growth factor receptor kinase activation in vivo. 
  • Noninvasive visualization of retinoblastoma growth and metastasis via bioluminescence imaging. 
  • Novel integrase-defective lentiviral episomal vectors for gene transfer. 
  • Novel luciferase-based reporter system to monitor activation of ErbB2/Her2/neu pathway noninvasively during radiotherapy. 
  • Nuclear factor erythroid-derived factor 2-related factor 2 regulates transcription of CCAAT/enhancer-binding protein β during adipogenesis. 
  • Nucleotide excision repair as a marker for susceptibility to tobacco-related cancers: a review of molecular epidemiological studies. 
  • Odor coding by a Mammalian receptor repertoire. 
  • Orphan nuclear receptor HNF-4 binds to the human coagulation factor VII promoter. 
  • Paracrine Wnt signaling both promotes and inhibits human breast tumor growth. 
  • Parathyroid hormone-related peptide represses chondrocyte hypertrophy through a protein phosphatase 2A/histone deacetylase 4/MEF2 pathway. 
  • Partial inhibition of gp130-Jak-Stat3 signaling prevents Wnt-β-catenin-mediated intestinal tumor growth and regeneration. 
  • Periodic root branching in Arabidopsis requires synthesis of an uncharacterized carotenoid derivative. 
  • Peutz-Jeghers LKB1 mutants fail to activate GSK-3beta, preventing it from inhibiting Wnt signaling. 
  • Polyphosphoramidate gene carriers: effect of charge group on gene transfer efficiency. 
  • Posttranscriptional regulation of protein expression in human epithelial carcinoma cells by adenine-uridine-rich elements in the 3'-untranslated region of tumor necrosis factor-alpha messenger RNA. 
  • Precise arrangement of factor-binding sites is required for murine CD4 promoter function. 
  • Problems for risk assessment of endocrine-active estrogenic compounds. 
  • Prolactin induction of insulin gene expression: the roles of glucose and glucose transporter-2. 
  • Prostaglandin A1 inhibits stress-induced NF-kappaB activation and reverses resistance to topoisomerase II inhibitors. 
  • Protein kinase C zeta transactivates hypoxia-inducible factor alpha by promoting its association with p300 in renal cancer. 
  • Pseudotyped viruses permit rapid detection of neutralizing antibodies in human and equine serum against Venezuelan equine encephalitis virus. 
  • Quantification of transforming growth factor-beta in biological material using cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct. 
  • Quantitative, noninvasive imaging of radiation-induced DNA double-strand breaks in vivo. 
  • Radiation-inducible caspase-8 gene therapy for malignant brain tumors. 
  • Rapid assessment of repair of ultraviolet DNA damage with a modified host-cell reactivation assay using a luciferase reporter gene and correlation with polymorphisms of DNA repair genes in normal human lymphocytes. 
  • Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein. 
  • Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. 
  • Regulation of E2A activities by histone acetyltransferases in B lymphocyte development. 
  • Regulation of MEF2 by histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications. 
  • Regulation of casein kinase I epsilon activity by Wnt signaling. 
  • Repair of mitomycin C cross-linked DNA in mammalian cells measured by a host cell reactivation assay. 
  • Repeated intrathecal administration of plasmid DNA complexed with polyethylene glycol-grafted polyethylenimine led to prolonged transgene expression in the spinal cord. 
  • Requirement for Abl kinases in T cell receptor signaling. 
  • Retinoic acid receptor beta2 inhibition of metastasis in mouse mammary gland xenografts. 
  • Role for ETS domain transcription factors Pea3/Erm in mouse lung development. 
  • Role for hedgehog signaling in hepatic stellate cell activation and viability. 
  • Selective activation of mitogen-activated protein (MAP) kinase kinase 3 and p38alpha MAP kinase is essential for cyclic AMP-dependent UCP1 expression in adipocytes. 
  • Sequence requirements for micro RNA processing and function in human cells. 
  • Sonic hedgehog signaling pathway supports cancer cell growth during cancer radiotherapy. 
  • Split luciferase complementation assay for the analysis of G protein-coupled receptor ligand response in Saccharomyces cerevisiae. 
  • Suppression of pancreatic tumor progression by systemic delivery of a pancreatic-cancer-specific promoter driven Bik mutant. 
  • Suppression of tumorigenesis and induction of p15(ink4b) by Smad4/DPC4 in human pancreatic cancer cells. 
  • Surface modified poly(β amino ester)-containing nanoparticles for plasmid DNA delivery. 
  • Synergistic and tunable human gene activation by combinations of synthetic transcription factors. 
  • Systemic dissemination of viral vectors during intratumoral injection. 
  • TGF-beta induces p65 acetylation to enhance bacteria-induced NF-kappaB activation. 
  • Targeting the lactate transporter MCT1 in endothelial cells inhibits lactate-induced HIF-1 activation and tumor angiogenesis. 
  • The C-terminal domain of A1/Bfl-1 regulates its anti-inflammatory function in human endothelial cells. 
  • The MEK pathway is required for stimulation of p21(WAF1/CIP1) by transforming growth factor-beta. 
  • The effect of the degree of chitosan deacetylation on the efficiency of gene transfection. 
  • The insecticide synergist piperonyl butoxide inhibits hedgehog signaling: assessing chemical risks. 
  • The need for enhancers in gene expression first appears during mouse development with formation of the zygotic nucleus. 
  • The putative tumor suppressor Cdx2 is overexpressed by human colorectal adenocarcinomas. 
  • The type III transforming growth factor-beta receptor negatively regulates nuclear factor kappa B signaling through its interaction with beta-arrestin2. 
  • Tolfenamic acid and pancreatic cancer growth, angiogenesis, and Sp protein degradation. 
  • Toxicity of cationic lipid-ribozyme complexes in human prostate tumor cells can mimic ribozyme activity. 
  • Transcriptional regulation of the human alpha1a-adrenergic receptor gene. Characterization Of the 5'-regulatory and promoter region. 
  • Transfection efficiency and transgene expression kinetics of mRNA delivered in naked and nanoparticle format. 
  • Transgene expression in the brain stem effected by intramuscular injection of polyethylenimine/DNA complexes. 
  • Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds. 
  • Use of bacterial quorum-sensing components to regulate gene expression in plants. 
  • Use of bioluminescence imaging to detect enhanced hepatic and systemic tumor growth following partial hepatectomy in mice. 
  • Use of in vivo bioluminescence imaging to predict hepatic tumor burden in mice. 
  • Variation in the miRNA-433 binding site of FGF20 confers risk for Parkinson disease by overexpression of alpha-synuclein. 
  • Ventricular expression of a MLC-2v-ras fusion gene induces cardiac hypertrophy and selective diastolic dysfunction in transgenic mice. 
  • WT1 and DAX-1 inhibit aromatase P450 expression in human endometrial and endometriotic stromal cells. 
  • zebraflash transgenic lines for in vivo bioluminescence imaging of stem cells and regeneration in adult zebrafish. 
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  Keywords of People

  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Choi, Steven Sok, Associate Professor of Medicine, Medicine, Gastroenterology
  • Hogan, Brigid L. M., George Barth Geller Distinguished Professor of Immunology, Cell Biology
  • Luftig, Micah Alan, Associate Professor of Molecular Genetics and Microbiology, Immunology
  • Milano, Carmelo Alessio, Professor of Surgery, Surgery, Cardiovascular and Thoracic Surgery