Luminescent Proteins

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  Subject Areas on Research

  • A GH3-like domain in reaper is required for mitochondrial localization and induction of IAP degradation. 
  • A Gαs DREADD mouse for selective modulation of cAMP production in striatopallidal neurons. 
  • A beta-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation. 
  • A broad competence to respond to SHORT ROOT revealed by tissue-specific ectopic expression. 
  • A cis-acting sequence homologous to the yeast filamentation and invasion response element regulates expression of a pectinase gene from the bean pathogen Colletotrichum lindemuthianum. 
  • A connection between stress and development in the multicellular prokaryote Streptomyces coelicolor A3(2). 
  • A cortico-hippocampal learning rule shapes inhibitory microcircuit activity to enhance hippocampal information flow. 
  • A cut above the other caspases. 
  • A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome. 
  • A functional antigenomic promoter for the paramyxovirus simian virus 5 requires proper spacing between an essential internal segment and the 3' terminus. 
  • A gene expression map of the Arabidopsis root. 
  • A multimodal micro-optrode combining field and single unit recording, multispectral detection and photolabeling capabilities. 
  • A novel conditionally replicative adenovirus vector targeting telomerase-positive tumor cells. 
  • A novel rodent mammary window of orthotopic breast cancer for intravital microscopy. 
  • A novel role for XIAP in copper homeostasis through regulation of MURR1. 
  • A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles. 
  • A regulatory cascade of three homeobox genes, ceh-10, ttx-3 and ceh-23, controls cell fate specification of a defined interneuron class in C. elegans. 
  • A role for ΔFosB in calorie restriction-induced metabolic changes. 
  • A tyrosine hydroxylase-yellow fluorescent protein knock-in reporter system labeling dopaminergic neurons reveals potential regulatory role for the first intron of the rodent tyrosine hydroxylase gene. 
  • Activation of Nrf2 Is Required for Normal and ChREBPα-Augmented Glucose-Stimulated β-Cell Proliferation. 
  • Activity-regulated cytoskeleton-associated protein Arc/Arg3.1 binds to spectrin and associates with nuclear promyelocytic leukemia (PML) bodies. 
  • Agonist-specific regulation of delta-opioid receptor trafficking by G protein-coupled receptor kinase and beta-arrestin. 
  • Alphavirus replicon particles as candidate HIV vaccines. 
  • An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. 
  • Analysis of the biologic properties of p230 Bcr-Abl reveals unique and overlapping properties with the oncogenic p185 and p210 Bcr-Abl tyrosine kinases. 
  • Anchor cell invasion into the vulval epithelium in C. elegans. 
  • Apparent loss-of-function mutant GPCRs revealed as constitutively desensitized receptors. 
  • Application of optical imaging and spectroscopy to radiation biology. 
  • Arc/Arg3.1 translation is controlled by convergent N-methyl-D-aspartate and Gs-coupled receptor signaling pathways. 
  • Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. 
  • Atrial chamber-specific expression of sarcolipin is regulated during development and hypertrophic remodeling. 
  • Binding of the beta2 adrenergic receptor to N-ethylmaleimide-sensitive factor regulates receptor recycling. 
  • Bioenergetic analysis of peroxisome proliferator-activated receptor gamma coactivators 1alpha and 1beta (PGC-1alpha and PGC-1beta) in muscle cells. 
  • Bone marrow-derived progenitor cells contribute to experimental choroidal neovascularization. 
  • CDK5 regulates cell adhesion and migration in corneal epithelial cells. 
  • CX3CR1 tyrosine sulfation enhances fractalkine-induced cell adhesion. 
  • Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity. 
  • Cell-Specific Chemical Delivery Using a Selective Nitroreductase-Nitroaryl Pair. 
  • Cellular trafficking of G protein-coupled receptor/beta-arrestin endocytic complexes. 
  • Computational visual ecology in the pelagic realm. 
  • Concentrated RD114-pseudotyped MFGS-gp91phox vector achieves high levels of functional correction of the chronic granulomatous disease oxidase defect in NOD/SCID/beta -microglobulin-/- repopulating mobilized human peripheral blood CD34+ cells. 
  • Constitutively active alpha-1b adrenergic receptor mutants display different phosphorylation and internalization features. 
  • Construction and detection of fluorescent, germline transgenic zebrafish. 
  • Covalent protein-oligonucleotide conjugates by copper-free click reaction. 
  • Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. 
  • Differential Requirements of TCR Signaling in Homeostatic Maintenance and Function of Dendritic Epidermal T Cells. 
  • Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein. 
  • Direct in vivo evidence for tumor propagation by glioblastoma cancer stem cells. 
  • Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity. 
  • Disrupted spermine homeostasis: a novel mechanism in polyglutamine-mediated aggregation and cell death. 
  • Disruption of Arp2/3 results in asymmetric structural plasticity of dendritic spines and progressive synaptic and behavioral abnormalities. 
  • Disulfide-mediated dimerization of L1 Ig domains. 
  • Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control. 
  • Domain unfolding plays a role in superfibronectin formation. 
  • Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex. 
  • Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants. 
  • Dynamic shuttling and intranuclear mobility of nuclear hormone receptors. 
  • Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein. 
  • Efficient and rapid induction of a chronic myelogenous leukemia-like myeloproliferative disease in mice receiving P210 bcr/abl-transduced bone marrow. 
  • Efficient gene delivery to pancreatic islets with ultrasonic microbubble destruction technology. 
  • Endocytosis of functional epidermal growth factor receptor-green fluorescent protein chimera. 
  • Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection. 
  • Enhanced inhibition of neointimal hyperplasia by genetically engineered endothelial progenitor cells. 
  • Expanded polyglutamine domain proteins bind neurofilament and alter the neurofilament network. 
  • Expanded polyglutamine stretches form an 'aggresome'. 
  • Expression of a Malassezia Codon Optimized mCherry Fluorescent Protein in a Bicistronic Vector. 
  • Feedback in the brainstem: an excitatory disynaptic pathway for control of whisking. 
  • Flow cytometric analysis of fluorescence resonance energy transfer: a tool for high-throughput screening of molecular interactions in living cells. 
  • Fluorescence resonance energy transfer analysis of cell surface receptor interactions and signaling using spectral variants of the green fluorescent protein. 
  • Forces drive basement membrane invasion in Caenorhabditis elegans . 
  • FtsZ from divergent foreign bacteria can function for cell division in Escherichia coli. 
  • Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins. 
  • GFP fusions to a microtubule motor protein to visualize meiotic and mitotic spindle dynamics in Drosophila. 
  • GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. 
  • Gateway-compatible tissue-specific vectors for plant transformation. 
  • Generation of a syngeneic mouse model to study the effects of vascular endothelial growth factor in ovarian carcinoma. 
  • Generation of neuronal intranuclear inclusions by polyglutamine-GFP: analysis of inclusion clearance and toxicity as a function of polyglutamine length. 
  • Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach. 
  • Glutamate decreases mitochondrial size and movement in primary forebrain neurons. 
  • Green fluorescent protein as a tag for molecular motor proteins. 
  • Heat-induced gene expression as a novel targeted cancer gene therapy strategy. 
  • High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates. 
  • Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization. 
  • Homo- and hetero-oligomerization of thyrotropin-releasing hormone (TRH) receptor subtypes. Differential regulation of beta-arrestins 1 and 2. 
  • How the kinetochore couples microtubule force and centromere stretch to move chromosomes. 
  • Human casein kinase Idelta phosphorylation of human circadian clock proteins period 1 and 2. 
  • Human substance P receptor lacking the C-terminal domain remains competent to desensitize and internalize. 
  • Hypoxia-regulated therapeutic gene as a preemptive treatment strategy against ischemia/reperfusion tissue injury. 
  • IL-4 induces differentiation and expansion of Th2 cytokine-producing eosinophils. 
  • Identification of Drosophila neuropeptide receptors by G protein-coupled receptors-beta-arrestin2 interactions. 
  • Identification of an inhibitory circuit that regulates cerebellar Golgi cell activity. 
  • Identification of the TBX5 transactivating domain and the nuclear localization signal. 
  • Imaging molecular pathways: reporter genes. 
  • In vivo bioluminescence imaging monitoring of hypoxia-inducible factor 1alpha, a promoter that protects cells, in response to chemotherapy. 
  • In vivo gene delivery and visualization of corneal stromal cells using an adenoviral vector and keratocyte-specific promoter. 
  • In vivo protein expression and immune responses generated by DNA vaccines expressing mycobacterial antigens fused with a reporter protein. 
  • Incorporation of heterologous outer membrane and periplasmic proteins into Escherichia coli outer membrane vesicles. 
  • Inhibition of polyglutamine protein aggregation and cell death by novel peptides identified by phage display screening. 
  • Initial stages of tumor cell-induced angiogenesis: evaluation via skin window chambers in rodent models. 
  • Inositol 1,4,5-trisphosphate receptor localization and stability in neonatal cardiomyocytes requires interaction with ankyrin-B. 
  • Interaction between TRPV1-expressing neurons in the hypothalamus. 
  • Intercellular Protein Transfer from Thymocytes to Thymic Epithelial Cells. 
  • Intravascular location of breast cancer cells after spontaneous metastasis to the lung. 
  • Intravital fluorescence facilitates measurement of multiple physiologic functions and gene expression in tumors of live animals. 
  • Katanin p60-like1 promotes microtubule growth and terminal dendrite stability in the larval class IV sensory neurons of Drosophila. 
  • Kin1 kinase localizes at the hyphal septum and is dephosphorylated by calcineurin but is dispensable for septation and virulence in the human pathogen Aspergillus fumigatus. 
  • Large Cellular Inclusions Accumulate in Arabidopsis Roots Exposed to Low-Sulfur Conditions. 
  • Large-scale death of retinal astrocytes during normal development is non-apoptotic and implemented by microglia. 
  • Ligand-selective interactions of ER detected in living cells by fluorescence resonance energy transfer. 
  • Liposome division by a simple bacterial division machinery. 
  • Maximal polar growth potential depends on the polarisome component AgSpa2 in the filamentous fungus Ashbya gossypii. 
  • Measurement of molecular interactions in living cells by fluorescence resonance energy transfer between variants of the green fluorescent protein. 
  • MicroRNA-mediated in vitro and in vivo direct reprogramming of cardiac fibroblasts to cardiomyocytes. 
  • Minimal determinants for binding activated G alpha from the structure of a G alpha(i1)-peptide dimer. 
  • Modulation of phagosome biogenesis by Legionella pneumophila creates an organelle permissive for intracellular growth. 
  • Molecular determinants underlying the formation of stable intracellular G protein-coupled receptor-beta-arrestin complexes after receptor endocytosis*. 
  • Molecular memory by reversible translocation of calcium/calmodulin-dependent protein kinase II. 
  • Molecular taxonomy of major neuronal classes in the adult mouse forebrain. 
  • Monitoring intracellular proteins using fluorescence techniques: from protein synthesis and localization to activity. 
  • Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer. 
  • Monitoring neural activity with bioluminescence during natural behavior. 
  • Myofibroblastic cells function as progenitors to regenerate murine livers after partial hepatectomy. 
  • Nerve activity-dependent modulation of calcineurin signaling in adult fast and slow skeletal muscle fibers. 
  • Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators. 
  • Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate. 
  • Neuronal cell shape and neurite initiation are regulated by the Ndr kinase SAX-1, a member of the Orb6/COT-1/warts serine/threonine kinase family. 
  • Novel integrase-defective lentiviral episomal vectors for gene transfer. 
  • Novel luciferase-based reporter system to monitor activation of ErbB2/Her2/neu pathway noninvasively during radiotherapy. 
  • Nuclear localization of NPR1 is required for activation of PR gene expression. 
  • O-glycosylation as a sorting determinant for cell surface delivery in yeast. 
  • Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells. 
  • Oligomerization of expanded-polyglutamine domain fluorescent fusion proteins in cultured mammalian cells. 
  • PDAPP; YFP double transgenic mice: a tool to study amyloid-beta associated changes in axonal, dendritic, and synaptic structures. 
  • Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe. 
  • Pericyte constriction underlies capillary derecruitment during hyperemia in the setting of arterial stenosis. 
  • Pericyte-like location of GFP-tagged melanoma cells: ex vivo and in vivo studies of extravascular migratory metastasis. 
  • Peritubular myoid cells are not the migrating population required for testis cord formation in the XY gonad. 
  • Persistent genetic instability in cancer cells induced by non-DNA-damaging stress exposures. 
  • Phenotypic characterization of transgenic Japanese medaka (Oryzias latipes) that express a red fluorescent protein in hepatocytes. 
  • Polyglutamine domain proteins with expanded repeats bind neurofilament, altering the neurofilament network. 
  • Presynaptic Inputs to Any CNS Projection Neuron Identified by Dual Recombinant Virus Infection. 
  • Preventing stem cell incorporation into choroidal neovascularization by targeting homing and attachment factors. 
  • Primary isolate neutralization by HIV type 1-infected patient sera in the era of highly active antiretroviral therapy. 
  • Primary mesenchyme cell patterning during the early stages following ingression. 
  • Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells. 
  • Protection against Mycobacterium avium by DNA vaccines expressing mycobacterial antigens as fusion proteins with green fluorescent protein. 
  • Quantum-dot-decorated robust transductable bioluminescent nanocapsules 
  • RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues. 
  • RANK, RANKL, OPG, and M-CSF expression in stromal cells during corneal wound healing. 
  • Radiation activates HIF-1 to regulate vascular radiosensitivity in tumors: role of reoxygenation, free radicals, and stress granules. 
  • Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. 
  • Rapid dendritic transport of TGN38, a putative cargo receptor. 
  • Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein. 
  • Re: initial stages of tumor cell-induced angiogenesis: evaluation via skin window chambers in rodent models. 
  • Real-time imaging of morphogenetic movements in Drosophila using Gal4-UAS-driven expression of GFP fused to the actin-binding domain of moesin. 
  • Regeneration Genetics. 
  • Regulated heat shock protein 27 expression in human retinal pigment epithelium. 
  • Regulation of ZAP-70 intracellular localization: visualization with the green fluorescent protein. 
  • Replication-Competent Influenza B Reporter Viruses as Tools for Screening Antivirals and Antibodies. 
  • Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis. 
  • Reprogramming after chromosome transfer into mouse blastomeres. 
  • Resolving postoperative neuroinflammation and cognitive decline. 
  • Resonating to the music of ubiquitination. 
  • Response features of parvalbumin-expressing interneurons suggest precise roles for subtypes of inhibition in visual cortex. 
  • Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus. 
  • Role of decreased levels of lipid phosphate phosphatase-1 in accumulation of lysophosphatidic acid in ovarian cancer. 
  • Rvs161p and sphingolipids are required for actin repolarization following salt stress. 
  • Saccharomyces cerevisiae Ntg1p and Ntg2p: broad specificity N-glycosylases for the repair of oxidative DNA damage in the nucleus and mitochondria. 
  • Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing. 
  • Signaling, desensitization, and trafficking of G protein-coupled receptors revealed by green fluorescent protein conjugates. 
  • Sorting human beta-cells consequent to targeted expression of green fluorescent protein. 
  • Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis. 
  • Spinal CCL2 Promotes Central Sensitization, Long-Term Potentiation, and Inflammatory Pain via CCR2: Further Insights into Molecular, Synaptic, and Cellular Mechanisms. 
  • Spindle dynamics during meiosis in Drosophila oocytes. 
  • Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain. 
  • Survival of retinal pigment epithelium after exposure to prolonged oxidative injury: a detailed gene expression and cellular analysis. 
  • Targeted activation of c-Jun N-terminal kinase in vivo induces restrictive cardiomyopathy and conduction defects. 
  • Targeted beta-adrenergic receptor kinase (betaARK1) inhibition by gene transfer in failing human hearts. 
  • Targeted inactivation of the isoprenylcysteine carboxyl methyltransferase gene causes mislocalization of K-Ras in mammalian cells. 
  • Targeting cells with single vectors using multiple-feature Boolean logic. 
  • Temporal activation of c-Jun N-terminal kinase in adult transgenic heart via cre-loxP-mediated DNA recombination. 
  • Temporally distinct and ligand-specific recruitment of nuclear receptor-interacting peptides and cofactors to subnuclear domains containing the estrogen receptor. 
  • The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. 
  • The DELLA motif is essential for gibberellin-induced degradation of RGA. 
  • The Early-Acting Peroxin PEX19 Is Redundantly Encoded, Farnesylated, and Essential for Viability in Arabidopsis thaliana. 
  • The ankyrin-B C-terminal domain determines activity of ankyrin-B/G chimeras in rescue of abnormal inositol 1,4,5-trisphosphate and ryanodine receptor distribution in ankyrin-B (-/-) neonatal cardiomyocytes. 
  • The beta2-adrenergic receptor/betaarrestin complex recruits the clathrin adaptor AP-2 during endocytosis. 
  • The cyclophilin-like domain of Ran-binding protein-2 modulates selectively the activity of the ubiquitin-proteasome system and protein biogenesis. 
  • The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome. 
  • The inducibly expressed GTPase localizes to the endoplasmic reticulum, independently of GTP binding. 
  • The molecular structure of green fluorescent protein. 
  • The stability of the G protein-coupled receptor-beta-arrestin interaction determines the mechanism and functional consequence of ERK activation. 
  • Tracing Tumor Evolution in Sarcoma Reveals Clonal Origin of Advanced Metastasis. 
  • Trafficking of yellow-fluorescent-protein-tagged mu1 subunit of clathrin adaptor AP-1 complex in living cells. 
  • Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination. 
  • Transfer of CD8+ T cell memory using Bcl-2 as a marker. 
  • Transgene expression in zebrafish: A comparison of retroviral-vector and DNA-injection approaches. 
  • Transgenic expression of green fluorescence protein can cause dilated cardiomyopathy. 
  • Tunable molecular tension sensors reveal extension-based control of vinculin loading. 
  • Tyrosine 1101 of Tie2 is the major site of association of p85 and is required for activation of phosphatidylinositol 3-kinase and Akt. 
  • Ubiquitous expression of mRFP1 in transgenic mice. 
  • Underwater linear polarization: physical limitations to biological functions. 
  • Using Tools from Optogenetics to Create Light-Responsive Biomaterials: LOVTRAP-PEG Hydrogels for Dynamic Peptide Immobilization. 
  • VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. 
  • Vascular control of kidney epithelial transporters. 
  • Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. 
  • Visualization of dynamic trafficking of a protein kinase C betaII/green fluorescent protein conjugate reveals differences in G protein-coupled receptor activation and desensitization. 
  • Zebrafish as a neurotoxicological model. 
  • [A novel model for visualization of tumor cell-induced angiogenesis in vivo]. 
  • beta-Arrestin-mediated PDE4 cAMP phosphodiesterase recruitment regulates beta-adrenoceptor switching from Gs to Gi. 
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  Keywords of People

  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Calakos, Nicole, Lincoln Financial Group Distinguished Professor of Neurobiology, Duke Science & Society
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Ferreira, Paulo Alexandre, Associate Professor in Ophthalmology, Pathology
  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Milano, Carmelo Alessio, Joseph W. and Dorothy W. Beard Distinguished Professor of Experimental Surgery, Surgery, Cardiovascular and Thoracic Surgery
  • Palmer, Gregory M., Associate Professor of Radiation Oncology, Radiation Oncology
  • Shinohara, Mari L., Professor of Integrative Immunobiology, Cell Biology
  • Soderling, Scott Haydn, George Barth Geller Distinguished Professor of Molecular Biology, Cell Biology
  • Tsalik, Ephraim, Adjunct Associate Professor in the Department of Medicine, Medicine, Infectious Diseases
  • Velkey, John Matthew, Assistant Professor of the Practice of Medical Education in the Department of Cell Biology, Cell Biology