Microtubules

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  Subject Areas on Research

  • 3-nitrotyrosine attenuates respiratory syncytial virus infection in human bronchial epithelial cell line. 
  • A Mec17-Myosin II Effector Axis Coordinates Microtubule Acetylation and Actin Dynamics to Control Primary Cilium Biogenesis. 
  • A PIK3C3-ankyrin-B-dynactin pathway promotes axonal growth and multiorganelle transport. 
  • A bidirectional kinesin motor in live Drosophila embryos. 
  • A cellular genome-wide association study reveals human variation in microtubule stability and a role in inflammatory cell death. 
  • A developmental mutation (npfL1) resulting in cell death in Physarum polycephalum. 
  • A kinesin motor in a force-producing conformation. 
  • A microtubule-destabilizing kinesin motor regulates spindle length and anchoring in oocytes. 
  • A model for fast axonal transport. 
  • A mutant of the motor protein kinesin that moves in both directions on microtubules. 
  • A new identity for MLK3 as an NIMA-related, cell cycle-regulated kinase that is localized near centrosomes and influences microtubule organization. 
  • A point mutation in the microtubule binding region of the Ncd motor protein reduces motor velocity. 
  • A specific domain of Gialpha required for the transactivation of Gialpha by tubulin is implicated in the organization of cellular microtubules. 
  • A structural pathway for activation of the kinesin motor ATPase. 
  • A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis. 
  • A tubulin binding peptide targets glioma cells disrupting their microtubules, blocking migration, and inducing apoptosis. 
  • Acrylamide and carbon disulfide treatments increase the rate of rat brain tubulin polymerization. 
  • Altered nucleotide-microtubule coupling and increased mechanical output by a kinesin mutant. 
  • Amyloid-β oligomers induce synaptic damage via Tau-dependent microtubule severing by TTLL6 and spastin. 
  • An N-terminal truncation of the ncd motor protein supports diffusional movement of microtubules in motility assays. 
  • An analysis of the response to gut induction in the C. elegans embryo. 
  • An ankyrin-based mechanism for functional organization of dystrophin and dystroglycan. 
  • An estimate to the first approximation of microtubule rupture force. 
  • Analysis of the regulation of microtubule dynamics by interaction of RGSZ1 (RGS20) with the neuronal stathmin, SCG10. 
  • Anastral spindle assembly: a mathematical model. 
  • Ankyrin-B interactions with spectrin and dynactin-4 are required for dystrophin-based protection of skeletal muscle from exercise injury. 
  • Ankyrin-G is a molecular partner of E-cadherin in epithelial cells and early embryos. 
  • Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation. 
  • Are cross-bridging structures involved in the bundle formation of intermediate filaments and the decrease in locomotion that accompany cell aging? 
  • Arl2- and Msps-dependent microtubule growth governs asymmetric division. 
  • Arrestin translocation is induced at a critical threshold of visual signaling and is superstoichiometric to bleached rhodopsin. 
  • Assays to measure plasma membrane and cytoskeletal changes in interferon-treated cells. 
  • Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes. 
  • Assembly of microtubules from preformed, ring-shaped protofilaments and 6-S tubulin. 
  • Assembly pathway of the anastral Drosophila oocyte meiosis I spindle. 
  • Asymmetric division: a kinesin for spindle positioning. 
  • Asymmetric spindle positioning. 
  • Bacterial cell division protein FtsZ assembles into protofilament sheets and minirings, structural homologs of tubulin polymers. 
  • Beyond taxanes: a review of novel agents that target mitotic tubulin and microtubules, kinases, and kinesins. 
  • Binding sites on microtubules of kinesin motors of the same or opposite polarity. 
  • Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells. 
  • Calmodulin as a mediator of hormone action and cell regulation. 
  • Calmodulin in endocrine cells and its multiple roles in hormone action. 
  • Calmodulin, cell growth and gene expression. 
  • Calmodulin--an intracellular calcium receptor. 
  • Calmodulin-microtubule association in cultured mammalian cells. 
  • Calmodulin: properties, intracellular localization, and multiple roles in cell regulation. 
  • Cell contacts orient some cell division axes in the Caenorhabditis elegans embryo. 
  • Cell division requires a direct link between microtubule-bound RacGAP and Anillin in the contractile ring. 
  • Cell polarity in sea urchin embryos: reorientation of cells occurs quickly in aggregates. 
  • Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae. 
  • Cellular events during sexual development from amoeba to plasmodium in the slime mould Physarum polycephalum. 
  • Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering. 
  • Checkpoint signals in grasshopper meiosis are sensitive to microtubule attachment, but tension is still essential. 
  • Conditional root expansion mutants of Arabidopsis. 
  • Control of microtubule assembly-disassembly by calcium-dependent regulator protein. 
  • Coordinating cytoskeletal tracks to polarize cellular movements. 
  • Crescerin uses a TOG domain array to regulate microtubules in the primary cilium. 
  • Current Progress in Electrotransfection as a Nonviral Method for Gene Delivery. 
  • Cyclic adenosine 3',5' monophosphate, calcium and protein phosphorylation in flagellar motility. 
  • Cytoplasmic dynein is required to oppose the force that moves nuclei towards the hyphal tip in the filamentous ascomycete Ashbya gossypii. 
  • DEPDC1/LET-99 participates in an evolutionarily conserved pathway for anti-tubulin drug-induced apoptosis. 
  • Decoupling of nucleotide- and microtubule-binding sites in a kinesin mutant. 
  • Definition of distinct compartments in polarized Madin-Darby canine kidney (MDCK) cells for membrane-volume sorting, polarized sorting and apical recycling. 
  • Desmoplakin: an unexpected regulator of microtubule organization in the epidermis. 
  • Determinants of kinesin motor polarity. 
  • Determinants of molecular motor directionality. 
  • Dexamethasone inhibits trabecular cell retraction. 
  • Diatom gliding is the result of an actin-myosin motility system. 
  • Differential localization patterns of septins during growth of the human fungal pathogen Aspergillus fumigatus reveal novel functions. 
  • Differential regulation of polarized synaptic vesicle trafficking and synapse stability in neural circuit rewiring in Caenorhabditis elegans. 
  • Differential uptake of DNA-poly(ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces. 
  • Dilution-induced disassembly of microtubules: relation to dynamic instability and the GTP cap. 
  • Direct ultrastructural evidence of target-directed polarization by cytotoxic lymphocytes in lesions of human graft-vs-host disease. 
  • Directionality and processivity of molecular motors. 
  • Disruption of the basal body compromises proteasomal function and perturbs intracellular Wnt response. 
  • Divergent regulation of functionally distinct γ-tubulin complexes during differentiation. 
  • Doublecortin-Like Kinases Promote Neuronal Survival and Induce Growth Cone Reformation via Distinct Mechanisms. 
  • Drosophila neuroblast asymmetric cell division: recent advances and implications for stem cell biology. 
  • Drosophila spastin regulates synaptic microtubule networks and is required for normal motor function. 
  • Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. 
  • Dynamic localization of C. elegans TPR-GoLoco proteins mediates mitotic spindle orientation by extrinsic signaling. 
  • Dynamic microtubules drive circuit rewiring in the absence of neurite remodeling. 
  • Dynamic positioning of mitotic spindles in yeast: role of microtubule motors and cortical determinants. 
  • Dynamin and FtsZ. Missing links in mitochondrial and bacterial division. 
  • Dysferlin, annexin A1, and mitsugumin 53 are upregulated in muscular dystrophy and localize to longitudinal tubules of the T-system with stretch. 
  • EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules. 
  • Effect of vanadate on intracellular distribution and function of 10-nm filaments. 
  • Effect of vinblastine on neurohypophysial and adenohypophysial microtubules. 
  • Effect of vinblastine on rat liver ultrastructure. 
  • Effect of vinblastine on the fine structure of the rat adrenal cortex. 
  • Effect of vitamin A on microtubules of rat brain and liver. 
  • Effects of colchicine and cytochalasin B on vasopressin- and cyclic adenosine monophosphate-induced changes in toad urinary bladder. 
  • Effects of downregulated HDAC6 expression on the proliferation of lung cancer cells. 
  • Effects of magnesium on the dynamic instability of individual microtubules. 
  • Effects of taxol and Colcemid on myofibrillogenesis. 
  • Effects of the src gene product on microfilament and microtubule organization in avian and mammalian cells infected with the same temperature-sensitive mutant of Rous sarcoma virus. 
  • Electron microscopy of MAP 2 (microtubule-associated protein 2). 
  • Electron-microscopic study of the apical region of the toad bladder epithelial cell. 
  • Embryonic polarity: a role for microtubules. 
  • Enhancement of the ncdD microtubule motor mutant by mutants of alpha Tub67C. 
  • Erythrocyte ankyrin: immunoreactive analogues are associated with mitotic structures in cultured cells and with microtubules in brain. 
  • Ethacrynic acid disrupts steady state microtubules in vitro. 
  • Evaluation of new anticancer agents against human pancreatic carcinomas in nude mice. 
  • Evolution in action: giant ankyrins awake. 
  • Expanded polyglutamine stretches form an 'aggresome'. 
  • Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy. 
  • FSH stimulation of protein kinase inhibitor: regulation of cellular metabolism. 
  • Fetal rat amniotic fluid: transforming growth factor beta and fibroblast collagen lattice contraction. 
  • Fibrillar systems in cell motility. 
  • Fluorescence recovery kinetic analysis of gamma-tubulin binding to the mitotic spindle. 
  • Function of microtubules under inadequate protein diet. 
  • Functions of cytoplasmic fibers in intracellular movements in BHK-21 cells. 
  • GTP hydrolysis during microtubule assembly. 
  • Gamma-tubulin nucleation: template or protofilament? 
  • Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport. 
  • Guanosinetriphosphatase activity of tubulin associated with microtubule assembly. 
  • HDAC6 is a microtubule-associated deacetylase. 
  • Histone deacetylase 6 regulates growth factor-induced actin remodeling and endocytosis. 
  • How calcium causes microtubule depolymerization. 
  • How cells get the right chromosomes. 
  • How the kinetochore couples microtubule force and centromere stretch to move chromosomes. 
  • Human ASPM participates in spindle organisation, spindle orientation and cytokinesis. 
  • Human apolipoprotein E accelerates microtubule polymerization in vitro. 
  • Hypothesis: microtubule instability and paired helical filament formation in the Alzheimer disease brain are related to apolipoprotein E genotype. 
  • IL-1beta decreases the elastic modulus of human tenocytes. 
  • Immunoreactive forms of erythrocyte spectrin and ankyrin in brain. 
  • Immunoreactive forms of human erythrocyte ankyrin are localized in mitotic structures in cultured cells and are associated with microtubules in brain. 
  • In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics. 
  • In vitro motility of AtKCBP, a calmodulin-binding kinesin protein of Arabidopsis. 
  • In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation. 
  • Increased calmodulin affects cell morphology and mRNA levels of cytoskeletal protein genes. 
  • Increased organization of cytoskeleton accompanying the aging of human fibroblasts in vitro. 
  • Influence of cytoskeletal assembly on phosphatidylcholine synthesis in intact phagocytic cells. 
  • Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules. 
  • Interaction of ethacrynic acid with bovine brain tubulin. 
  • Interferon suppresses pinocytosis but stimulates phagocytosis in mouse peritoneal macrophages: related changes in cytoskeletal organization. 
  • Involvement of microtubules and 10-nm filaments in the movement and positioning of nuclei in syncytia. 
  • Kar3 interaction with Cik1 alters motor structure and function. 
  • Katanin p60-like1 promotes microtubule growth and terminal dendrite stability in the larval class IV sensory neurons of Drosophila. 
  • Kinesin proteins: a phylum of motors for microtubule-based motility. 
  • Large conformational changes in a kinesin motor catalyzed by interaction with microtubules. 
  • Lipid-based microtubular drug delivery vehicles. 
  • Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis. 
  • Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. 
  • Loss of BBS proteins causes anosmia in humans and defects in olfactory cilia structure and function in the mouse. 
  • Mammalian septins regulate microtubule stability through interaction with the microtubule-binding protein MAP4. 
  • Mature Drosophila meiosis I spindles comprise microtubules of mixed polarity. 
  • Mechanistic analysis of the Saccharomyces cerevisiae kinesin Kar3. 
  • Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin. 
  • Meiosis, mitosis and microtubule motors. 
  • Messenger ribonucleoprotein complexes containing human ELAV proteins: interactions with cytoskeleton and translational apparatus. 
  • Microtubule acetylation amplifies p38 kinase signalling and anti-inflammatory IL-10 production. 
  • Microtubule binding to Smads may regulate TGF beta activity. 
  • Microtubule disruption leads to cellular contraction in human trabecular meshwork cells. 
  • Microtubule dynamic instability and GTP hydrolysis. 
  • Microtubule nucleation and organization in teleost photoreceptors: microtubule recovery after elimination by cold. 
  • Microtubule organization, dynamics and functions in differentiated cells. 
  • Microtubule organization: cell shape is destiny. 
  • Microtubule stabilization and potentiation of taxol activity by the creatine analog cyclocreatine. 
  • Microtubule structure at 18 A resolution. 
  • Microtubule surface lattice and subunit structure and observations on reassembly. 
  • Microtubule transport defects in neurological and ciliary disease. 
  • Microtubule treadmilling in vitro investigated by fluorescence speckle and confocal microscopy. 
  • Microtubules accelerate the kinase activity of Aurora-B by a reduction in dimensionality. 
  • Molecular and cellular mechanisms of leukocyte chemotaxis. 
  • Morphological, biochemical, and genetic support for an apolipoprotein E effect on microtubular metabolism. 
  • Motor proteins 1: kinesins. 
  • Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis. 
  • Mutations in Either TUBB or MAPRE2 Cause Circumferential Skin Creases Kunze Type. 
  • Neck-motor interactions trigger rotation of the kinesin stalk. 
  • Noncentrosomal microtubules and type II myosins potentiate epidermal cell adhesion and barrier formation. 
  • Normal spastin gene dosage is specifically required for axon regeneration. 
  • Not just another hole in the wall: understanding intercellular protein trafficking. 
  • Novel chemotherapies in development for management of castration-resistant prostate cancer. 
  • NuMA-microtubule interactions are critical for spindle orientation and the morphogenesis of diverse epidermal structures. 
  • Nucleation of microtubule assembly. Experimental kinetics, computer fitting of models, and observations on tubulin rings. 
  • PAR proteins regulate microtubule dynamics at the cell cortex in C. elegans. 
  • Phorbol myristate acetate stimulates microtubule and 10-nm filament extension and lysosome redistribution in mouse macrophages. 
  • Physiological implications of the presence, distribution, and regulation of calmodulin in eukaryotic cells. 
  • Plasmids for expression of chimeric and truncated kinesin proteins. 
  • Polycation-induced assembly of purified tubulin. 
  • Posttranslational modifications of tubulin in teleost photoreceptor cytoskeletons. 
  • Predicting Division Planes of Three-Dimensional Cells by Soap-Film Minimization. 
  • Prenylation-dependent association of Ki-Ras with microtubules. Evidence for a role in subcellular trafficking. 
  • Protein inhibitor of cAMP-dependent protein kinase: production and characterization of antibodies and intracellular localization. 
  • Protofilaments and rings, two conformations of the tubulin family conserved from bacterial FtsZ to alpha/beta and gamma tubulin. 
  • RANBP2 is an allosteric activator of the conventional kinesin-1 motor protein, KIF5B, in a minimal cell-free system. 
  • Radial migration: Retinal neurons hold on for the ride. 
  • Rapid double 8-nm steps by a kinesin mutant. 
  • Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer. 
  • Rapid purification of microtubule motor domain proteins expressed in bacteria. 
  • Rapid up-regulation of peptide elongation factor EF-1alpha protein levels is an immediate early event during oxidative stress-induced apoptosis. 
  • Regulation by and of calmodulin in mammalian cells. 
  • Regulation of microfilaments and microtubules by calcium and cyclic AMP. 
  • Regulation of the cytoskeleton by Ca2+-calmodulin and cAMP. 
  • Regulation of the postsynaptic cytoskeleton: roles in development, plasticity, and disorders. 
  • Regulation of the testis sertoli cell by follicle stimulating hormone. 
  • Relationship between movement and aggregation of centrioles in syncytia and formation of microtubule bundles. 
  • Serum retinol binding protein secretion through microtubules in chicks. 
  • Small molecule intervention in microtubule-associated human disease. 
  • Src-mediated tyrosine phosphorylation of dynamin is required for beta2-adrenergic receptor internalization and mitogen-activated protein kinase signaling. 
  • Stabilizing electrode-host interfaces: a tissue engineering approach. 
  • Structural and functional domains of the Drosophila ncd microtubule motor protein. 
  • Structure and function of the visual arrestin oligomer. 
  • Structure of microtubules with reduced hydration. Comparison of results from X-ray diffraction and electron microscopy. 
  • Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium-sequestering system. 
  • Subcellular changes in capillary endothelial cells during repair of hyperoxic lung injury. 
  • Subcellular localization of spastin: implications for the pathogenesis of hereditary spastic paraplegia. 
  • Sustained release of BMP-2 in a lipid-based microtube vehicle. 
  • T-tubule depolarization-induced SR Ca2+ release is controlled by dihydropyridine receptor- and Ca(2+)-dependent mechanisms in cell homogenates from rabbit skeletal muscle. 
  • Tau is essential to beta -amyloid-induced neurotoxicity. 
  • Tau phosphorylation by diisopropyl phosphorofluoridate (DFP)-treated hen brain supernatant inhibits its binding with microtubules: role of Ca2+/Calmodulin-dependent protein kinase II in tau phosphorylation. 
  • Tension on chromosomes increases the number of kinetochore microtubules but only within limits. 
  • The Bardet-Biedl protein BBS4 targets cargo to the pericentriolar region and is required for microtubule anchoring and cell cycle progression. 
  • The C-terminal polylysine region and methylation of K-Ras are critical for the interaction between K-Ras and microtubules. 
  • The Drosophila claret segregation protein is a minus-end directed motor molecule. 
  • The Drosophila ncd microtubule motor protein is spindle-associated in meiotic and mitotic cells. 
  • The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress. 
  • The discovery of the prokaryotic cytoskeleton: 25th anniversary. 
  • The effect of halothane on cultured fibroblasts and neuroblastoma cells. 
  • The effects of tri-o-cresyl phosphate and metabolites on rat Sertoli cell function in primary culture. 
  • The forces that position a mitotic spindle asymmetrically are tethered until after the time of spindle assembly. 
  • The interaction of RGSZ1 with SCG10 attenuates the ability of SCG10 to promote microtubule disassembly. 
  • The kinetics of microtubule assembly. Evidence for a two-stage nucleation mechanism. 
  • The marriage of quantitative genetics and cell biology: a novel screening approach reveals people have genetically encoded variation in microtubule stability. 
  • The microtubule-associated histone deacetylase 6 (HDAC6) regulates epidermal growth factor receptor (EGFR) endocytic trafficking and degradation. 
  • The minus end-directed motor Kar3 is required for coupling dynamic microtubule plus ends to the cortical shmoo tip in budding yeast. 
  • The prevalence of abnormal cilia in normal pediatric lungs. 
  • The role of actin in spindle orientation changes during the Saccharomyces cerevisiae cell cycle. 
  • The role of subunit entropy in cooperative assembly. Nucleation of microtubules and other two-dimensional polymers. 
  • The role of the cytoskeleton in aging. 
  • The structure and assembly of microtubules. 
  • The synergistic combination of the farnesyl transferase inhibitor lonafarnib and paclitaxel enhances tubulin acetylation and requires a functional tubulin deacetylase. 
  • The tail domain of the Aspergillus fumigatus class V myosin MyoE orchestrates septal localization and hyphal growth. 
  • The use of lipid microtubes as a novel slow-release delivery system for laryngeal injection. 
  • Thermodynamic and kinetic aspects of assembly. 
  • To excite a heart: a bird's view. 
  • Tubular arrays on electron microscopy not specific. 
  • Tubulin and calmodulin. Effects of microtubule and microfilament inhibitors on localization in the mitotic apparatus. 
  • Tubulin rings: curved filaments with limited flexibility and two modes of association. 
  • Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster. 
  • Two Small Molecules Block Oral Epithelial Cell Invasion by Porphyromons gingivalis. 
  • Two new loci, PLEIADE and HYADE, implicate organ-specific regulation of cytokinesis in Arabidopsis. 
  • Two-state displacement by the kinesin-14 Ncd stalk. 
  • Ultrastructure of heart muscle. 
  • Unravelling the tangled web at the microtubule-organizing center. 
  • Visualization of slow axonal transport in vivo. 
  • Visualizing Intracellular Organelle and Cytoskeletal Interactions at Nanoscale Resolution on Millisecond Timescales. 
  • XMAP215 is a long thin molecule that does not increase microtubule stiffness. 
  • Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. 
  • n-ethylmaleimide and ethacrynic acid inhibit kinesin binding to microtubules in a motility assay. 
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  Keywords of People

  • Bennett, Vann, George B. Geller Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute
  • Erickson, Harold Paul, James B. Duke Professor of Cell Biology, Biomedical Engineering