Myosins

• 

  Subject Areas on Research

  • A mutation in TNNC1-encoded cardiac troponin C, TNNC1-A31S, predisposes to hypertrophic cardiomyopathy and ventricular fibrillation. 
  • A new structural state of myosin. 
  • Abelson kinase (Abl) and RhoGEF2 regulate actin organization during cell constriction in Drosophila. 
  • Actin and myosin biochemistry in relation to cytokinesis. 
  • Actin and myosin function in acanthamoeba. 
  • Antibody inhibitors of nonmuscle myosin function and assembly. 
  • Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation. 
  • Association of myosin light chain kinase with lymphocyte membrane-cytoskeleton complex. 
  • Atypical myosin heavy chain in rat laryngeal muscle. 
  • Axonal transport of actin in rabbit retinal ganglion cells. 
  • Binding of ADP and adenosine 5'-[beta, gamma-imido]triphosphate to insect flight muscle fibrils. 
  • Binding of myosin subfragment 1 to glycerinated insect flight muscle in the rigor state. 
  • Biochemical comparison of fast- and slow-contracting squid muscle. 
  • Calmodulin in endocrine cells and its multiple roles in hormone action. 
  • Cell mechanics: dissecting the physical responses of cells to force. 
  • Characterization of monoclonal antibodies to Acanthamoeba myosin-I that cross-react with both myosin-II and low molecular mass nuclear proteins. 
  • Characterization of myosin heavy chain by cyanogen bromide peptide maps. 
  • Contractile proteins in Drosophila development. 
  • Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension. 
  • Coordinate changes in C protein and myosin expression during skeletal muscle hypertrophy. 
  • Coordinate synthesis of two myosins in wild-type and mutant nematode muscle during larval development. 
  • Cross-bridge number, position, and angle in target zones of cryofixed isometrically active insect flight muscle. 
  • Crossbridges in the complete unit cell of rigor insect flight muscle imaged by three-dimensional reconstruction from oblique sections. 
  • Cytoplasmic myosin from Drosophila melanogaster. 
  • Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle. 
  • Diatom gliding is the result of an actin-myosin motility system. 
  • Different dimerization activities of alpha and beta thyroid hormone receptor isoforms. 
  • Differential gene expression in pubococcygeus muscle from patients with pelvic organ prolapse. 
  • Dimerized Drosophila myosin VIIa: a processive motor. 
  • Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization. 
  • Direct localization of monoclonal antibody-binding sites on Acanthamoeba myosin-II and inhibition of filament formation by antibodies that bind to specific sites on the myosin-II tail. 
  • Directionality and processivity of molecular motors. 
  • Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis. 
  • Drosophila crinkled, mutations of which disrupt morphogenesis and cause lethality, encodes fly myosin VIIA. 
  • Drosophila nonmuscle myosin II has multiple essential roles in imaginal disc and egg chamber morphogenesis. 
  • Dynamic changes in the distribution of cytoplasmic myosin during Drosophila embryogenesis. 
  • Effect of Mts1 on the structure and activity of nonmuscle myosin II. 
  • Effect of maturational changes in myosin content and morphometry on airway smooth muscle contraction. 
  • Effect of microRNA modulation on bioartificial muscle function. 
  • Effects of taxol and Colcemid on myofibrillogenesis. 
  • Effects of topical glaucoma drugs on fistulized rabbit conjunctiva. 
  • Electron microscopy of thin filaments decorated with a Ca2+-regulated myosin. 
  • Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions. 
  • Electron tomography of fast frozen, stretched rigor fibers reveals elastic distortions in the myosin crossbridges. 
  • Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. 
  • Enhanced myocardial function in transgenic mice overexpressing the beta 2-adrenergic receptor. 
  • Essential light chain of Drosophila nonmuscle myosin II. 
  • Evidence that myosin does not contribute to force production in chromosome movement. 
  • Expression of a novel combination of fast and slow troponin T isoforms in rabbit extraocular muscles. 
  • Expression of dominant negative Rho-binding domain of Rho-kinase in organ cultured human eye anterior segments increases aqueous humor outflow. 
  • Flightin is essential for thick filament assembly and sarcomere stability in Drosophila flight muscles. 
  • Fluorescence changes on contractile activation in TnC(DANZ) labeled skinned rabbit psoas fibers. 
  • Fluorine-18-antimyosin monoclonal antibody fragments: preliminary investigations in a canine myocardial infarct model. 
  • Force generation by kinesin and myosin cytoskeletal motor proteins. 
  • Functions of the myosin ATP and actin binding sites are required for C. elegans thick filament assembly. 
  • Gender-specific differences in expression of mRNAs for functional and structural proteins in rat ventricular myocardium. 
  • Gene expression in the rectus abdominus muscle of patients with and without pelvic organ prolapse. 
  • Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis. 
  • Genetic approaches to molecular motors. 
  • Genetic contribution to variation in DNA methylation at maternal smoking-sensitive loci in exposed neonates. 
  • Geometrical constraints affecting crossbridge formation in insect flight muscle. 
  • Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration. 
  • Glucocorticoids accelerate the ontogenetic transition of cardiac ventricular myosin heavy-chain isoform expression in the rat: promotion by prenatal exposure to a low dose of dexamethasone. 
  • Gold/Fab immuno electron microscopy localization of troponin H and troponin T in Lethocerus flight muscle. 
  • Hepatitis-C-virus-induced microRNAs dampen interferon-mediated antiviral signaling. 
  • Human erythrocyte clathrin and clathrin-uncoating protein. 
  • Human erythrocyte myosin: identification and purification. 
  • Human nonsyndromic hereditary deafness DFNA17 is due to a mutation in nonmuscle myosin MYH9. 
  • Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites. 
  • Identification of the functionally relevant calmodulin binding site in smooth muscle caldesmon. 
  • Identification of the gene for fly non-muscle myosin heavy chain: Drosophila myosin heavy chains are encoded by a gene family. 
  • Immunocytochemical localization of two myosins within the same muslce cells in Caenorhabditis elegans. 
  • In vitro studies of determinants of smooth muscle mechanics. 
  • Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy. 
  • Integrin-linked kinase: a possible role in scar contracture. 
  • Intraoperative floppy iris syndrome: report of a case and histopathologic analysis. 
  • Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis. 
  • Kinesin: switch I & II and the motor mechanism. 
  • Light-triggered myosin activation for probing dynamic cellular processes. 
  • MRCK-1 Drives Apical Constriction in C. elegans by Linking Developmental Patterning to Force Generation. 
  • Marked enhancement in myocardial function resulting from overexpression of a human beta-adrenergic receptor gene. 
  • Modeling rigor cross-bridge patterns in muscle I. Initial studies of the rigor lattice of insect flight muscle. 
  • Modulation of aqueous humor outflow facility by the Rho kinase-specific inhibitor Y-27632. 
  • Molecular genetic dissection of myosin heavy chain function. 
  • Monoclonal antibodies demonstrate limited structural homology between myosin isozymes from Acanthamoeba. 
  • Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function. 
  • Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy. 
  • Myosin I contributes to the generation of resting cortical tension. 
  • Myosin VIIA defects, which underlie the Usher 1B syndrome in humans, lead to deafness in Drosophila. 
  • Myosin VIIA, important for human auditory function, is necessary for Drosophila auditory organ development. 
  • Myosin filament structure and myosin crossbridge dynamics in fish and insect muscles. 
  • Myosin from human erythrocytes. 
  • Myosin head configuration in relaxed insect flight muscle: x-ray modeled resting cross-bridges in a pre-powerstroke state are poised for actin binding. 
  • Myosin heavy-chain mutations that disrupt Caenorhabditis elegans thick filament assembly. 
  • Myosin light chain-2 luciferase transgenic mice reveal distinct regulatory programs for cardiac and skeletal muscle-specific expression of a single contractile protein gene. 
  • Myosin-actin motors: the partnership goes atomic. 
  • Myosins exist as homodimers of heavy chains: demonstration with specific antibody purified by nematode mutant myosin affinity chromatography. 
  • Native nonmuscle myosin II stability and light chain binding in Drosophila melanogaster. 
  • Novel compound heterozygous mutations in MYO7A in a Chinese family with Usher syndrome type 1. 
  • Oblique section 3-D reconstruction of relaxed insect flight muscle reveals the cross-bridge lattice in helical registration. 
  • Ontogenetic transition of cardiac myosin heavy chain isoforms in rat ventricle: effects of fetal exposure to beta-adrenergic agonists or antagonists. 
  • Orotic acid improves left ventricular recovery four days after heterotopic transplantation. 
  • Overexpression of integrin alphav promotes human osteosarcoma cell populated collagen lattice contraction and cell migration. 
  • Patterns of troponin T expression in mammalian fast, slow and promiscuous muscle fibres. 
  • Phosphorylation of myosin-II regulatory light chain by cyclin-p34cdc2: a mechanism for the timing of cytokinesis. 
  • Predicted effects of nitric oxide and superoxide on the vasoactivity of the afferent arteriole. 
  • Promiscuous expression of myosin in myotonic dystrophy. 
  • Protein kinase C phosphorylates nonmuscle myosin-II heavy chain from Drosophila but regulation of myosin function by this enzyme is not required for viability in flies. 
  • Purification and characterization of the mammalian myosin light chain phosphatase holoenzyme. The differential effects of the holoenzyme and its subunits on smooth muscle. 
  • ROCK1 phosphorylates and activates zipper-interacting protein kinase. 
  • Red blood cell and fibroblast membranes in Duchenne and myotonic muscular dystrophy. 
  • Reentry into the cell cycle of differentiated skeletal myocytes. 
  • Response to acetylcholine and myosin content of isolated canine airways. 
  • Reverse actin sliding triggers strong myosin binding that moves tropomyosin. 
  • Reversible unfolding of fibronectin type III and immunoglobulin domains provides the structural basis for stretch and elasticity of titin and fibronectin. 
  • Rigor crossbridge structure in tilted single filament layers and flared-X formations from insect flight muscle. 
  • Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization. 
  • Sequence divergence of coiled coils--structural rods, myosin filament packing, and the extraordinary conservation of cohesins. 
  • Single-headed myosin II acts as a dominant negative mutation in Dictyostelium. 
  • Sites of interaction between kinase-related protein and smooth muscle myosin. 
  • Skeletal muscle adaptation to chronic low-frequency motor nerve stimulation. 
  • Soluble miniagrin enhances contractile function of engineered skeletal muscle. 
  • Springs and hinges: dynamic coiled coils and discontinuities. 
  • Stimulation of Acanthamoeba actomyosin ATPase activity by myosin-II polymerization. 
  • Structural studies on the conformations of myosin. 
  • Structure of myosin filaments from relaxed Lethocerus flight muscle by cryo-EM at 6 Å resolution. 
  • The actin membrane skeleton in Drosophila development. 
  • The human extraocular muscle myosin heavy chain gene (MYH13) maps to the cluster of fast and developmental myosin genes on chromosome 17. 
  • The myosin filament superlattice in the flight muscles of flies: A-band lattice optimisation for stretch-activation? 
  • The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila. 
  • The role of F-actin and myosin in epithelial cell rheology. 
  • The role of calmodulin in the structure and regulation of phosphorylase kinase from rabbit skeletal muscle. 
  • The superfast extraocular myosin (MYH13) is localized to the innervation zone in both the global and orbital layers of rabbit extraocular muscle. 
  • Three-dimensional reconstruction of rigor insect flight muscle from tilted thin sections. 
  • Tissue interactions pattern the mesenchyme of the embryonic mouse lung. 
  • Triggering a cell shape change by exploiting preexisting actomyosin contractions. 
  • Triiodothyronine (T3) decreases binding to DNA by T3-receptor homodimers but not receptor-auxiliary protein heterodimers. 
  • Two attached non-rigor crossbridge forms in insect flight muscle. 
  • Unified biophysical mechanism for cell-shape oscillations and cell ingression. 
  • Unraveling the mysteries of chaperone interactions of the myosin head. 
  • Vascular biology and invasive cardiology. The streams are beginning to merge. 
  • Wound healing: The power of the purse string. 
  • X-ray diffraction indicates that active cross-bridges bind to actin target zones in insect flight muscle. 
  • X-ray diffraction studies of striated muscles. 
  • X-ray evidence that in contracting live frog muscles there exist two distinct populations of myosin heads. 
  • ZIP kinase, a key regulator of myosin protein phosphatase 1. 
• 

  Keywords of People

  • Bejsovec, Amy, Associate Professor of Biology, Biology
  • Bennett, Vann, George B. Geller Distinguished Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute
  • Erickson, Harold Paul, James B. Duke Distinguished Professor of Cell Biology, Biomedical Engineering
  • Milano, Carmelo Alessio, Professor of Surgery, Surgery, Cardiovascular and Thoracic Surgery