Nucleic Acid Heteroduplexes

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  Subject Areas on Research

  • A naturally occurring hPMS2 mutation can confer a dominant negative mutator phenotype. 
  • Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae. 
  • Atomic force microscopy captures the initiation of methyl-directed DNA mismatch repair. 
  • Bidirectional excision in methyl-directed mismatch repair. 
  • Boranophosphates support the RNase H cleavage of polyribonucleotides. 
  • Characterization of a defective phage system for the analysis of bacteriophage T4 DNA replication origins. 
  • Conversion-type and restoration-type repair of DNA mismatches formed during meiotic recombination in Saccharomyces cerevisiae. 
  • Cytosine deamination in mismatched base pairs. 
  • DHFR/MSH3 amplification in methotrexate-resistant cells alters the hMutSalpha/hMutSbeta ratio and reduces the efficiency of base-base mismatch repair. 
  • DNA chain length dependence of formation and dynamics of hMutSalpha.hMutLalpha.heteroduplex complexes. 
  • DNA mismatches reveal conformational penalties in protein-DNA recognition. 
  • DNA polymerase delta is required for human mismatch repair in vitro. 
  • DNA strand-exchange patterns associated with double-strand break-induced and spontaneous mitotic crossovers in Saccharomyces cerevisiae. 
  • DNA-dependent activation of the hMutSalpha ATPase. 
  • Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair. 
  • Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases. 
  • Distinct MutS DNA-binding modes that are differentially modulated by ATP binding and hydrolysis. 
  • Elongation by Drosophila RNA polymerase II. Transcription of 3'-extended DNA templates. 
  • Extensive recombination due to heteroduplexes generates large amounts of artificial gene fragments during PCR. 
  • Frameshift intermediates in homopolymer runs are removed efficiently by yeast mismatch repair proteins. 
  • Genetic evidence for preferential strand transfer during meiotic recombination in yeast. 
  • Genetic evidence that the meiotic recombination hotspot at the HIS4 locus of Saccharomyces cerevisiae does not represent a site for a symmetrically processed double-strand break. 
  • Heteroduplex DNA position defines the roles of the Sgs1, Srs2, and Mph1 helicases in promoting distinct recombination outcomes. 
  • High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast. 
  • Human strand-specific mismatch repair occurs by a bidirectional mechanism similar to that of the bacterial reaction. 
  • Hypermutability and mismatch repair deficiency in RER+ tumor cells. 
  • Identifying sequence similarities between DNA molecules. 
  • Initiation of methyl-directed mismatch repair. 
  • Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells. 
  • Measurements of excision repair tracts formed during meiotic recombination in Saccharomyces cerevisiae. 
  • Meiotic recombination between dispersed repeated genes is associated with heteroduplex formation. 
  • Meiotic recombination involving heterozygous large insertions in Saccharomyces cerevisiae: formation and repair of large, unpaired DNA loops. 
  • Meiotic recombination within the centromere of a yeast chromosome. 
  • Methyl-directed mismatch repair is bidirectional. 
  • Methyl-directed repair of DNA base-pair mismatches in vitro. 1983. 
  • Microsatellite instability in yeast: dependence on repeat unit size and DNA mismatch repair genes. 
  • Misincorporation and mispaired primer extension by human immunodeficiency virus reverse transcriptase. 
  • Mismatch recognition and subsequent processing have distinct effects on mitotic recombination intermediates and outcomes in yeast. 
  • Mismatch repair in replication fidelity, genetic recombination, and cancer biology. 
  • Mismatch repair proteins regulate heteroduplex formation during mitotic recombination in yeast. 
  • Mismatch repair-dependent iterative excision at irreparable O6-methylguanine lesions in human nuclear extracts. 
  • Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex. 
  • Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein. 
  • Mispair specificity of methyl-directed DNA mismatch correction in vitro. 
  • Modulation of MutS ATP hydrolysis by DNA cofactors. 
  • Molecular structures of crossover and noncrossover intermediates during gap repair in yeast: implications for recombination. 
  • MutS and MutL activate DNA helicase II in a mismatch-dependent manner. 
  • MutS mediates heteroduplex loop formation by a translocation mechanism. 
  • Mutation detection with MutH, MutL, and MutS mismatch repair proteins. 
  • Mutation of MSH3 in endometrial cancer and evidence for its functional role in heteroduplex repair. 
  • Nonlinear interferometric vibrational imaging. 
  • Novel PMS1 alleles preferentially affect the repair of primer strand loops during DNA replication. 
  • Patterns of heteroduplex formation associated with the initiation of meiotic recombination in the yeast Saccharomyces cerevisiae. 
  • RNA-DNA hybrid formation at a bacteriophage T4 replication origin. 
  • Recognition and repair of compound DNA lesions (base damage and mismatch) by human mismatch repair and excision repair systems. 
  • Regulation of hetDNA Length during Mitotic Double-Strand Break Repair in Yeast. 
  • Removal of N-6-methyladenine by the nucleotide excision repair pathway triggers the repair of mismatches in yeast gap-repair intermediates. 
  • Repair of DNA loops involves DNA-mismatch and nucleotide-excision repair proteins. 
  • Repair of specific base pair mismatches formed during meiotic recombination in the yeast Saccharomyces cerevisiae. 
  • Role of proliferating cell nuclear antigen interactions in the mismatch repair-dependent processing of mitotic and meiotic recombination intermediates in yeast. 
  • Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines. 
  • The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex. 
  • The putative glucose 6-phosphate translocase gene is mutated in essentially all cases of glycogen storage disease type I non-a. 
  • The role of the mismatch repair machinery in regulating mitotic and meiotic recombination between diverged sequences in yeast. 
  • Tissue-specific expression of kallikrein-related genes in the rat. 
  • Visualizing transient Watson-Crick-like mispairs in DNA and RNA duplexes. 
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  Keywords of People

  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Kearney, Hutton Moore, Assistant Consulting Professor in the Department of Pathology, Pathology