Oocytes

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  Subject Areas on Research

  • A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis. 
  • A charged residue in S4 regulates coupling among the activation gate, voltage, and Ca2+ sensors in BK channels. 
  • A feedback loop in the polo-like kinase activation pathway. 
  • A grafted ovarian fragment rescues host fertility after chemotherapy. 
  • A microtubule-destabilizing kinesin motor regulates spindle length and anchoring in oocytes. 
  • A mutation in the glycine binding pocket of the N-methyl-D-aspartate receptor NR1 subunit alters agonist efficacy. 
  • A role for Cdc2- and PP2A-mediated regulation of Emi2 in the maintenance of CSF arrest. 
  • A tarantula-venom peptide antagonizes the TRPA1 nociceptor ion channel by binding to the S1-S4 gating domain. 
  • A three-generation approach in biodemography is based on the developmental profiles and the epigenetics of female gametes. 
  • Across the meiotic divide - CSF activity in the post-Emi2/XErp1 era. 
  • Actin dynamics: the arp2/3 complex branches out. 
  • Activation of the cloned muscarinic potassium channel by G protein beta gamma subunits. 
  • Activation-dependent subconductance levels in the drk1 K channel suggest a subunit basis for ion permeation and gating. 
  • Advanced maternal age and perinatal outcome: oocyte recipiency versus natural conception. 
  • Allosteric effects of external K+ ions mediated by the aspartate of the GYGD signature sequence in the Kv2.1 K+ channel. 
  • Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev. 
  • An alanine residue in the M3-M4 linker lines the glycine binding pocket of the N-methyl-D-aspartate receptor. 
  • An epilepsy/dyskinesia-associated mutation enhances BK channel activation by potentiating Ca2+ sensing. 
  • Analysis of the cell cycle using Xenopus egg extracts. 
  • Anastral spindle assembly and γ-tubulin in Drosophila oocytes. 
  • Anastral spindle assembly: a mathematical model. 
  • Aquaglyceroporin AQP9: solute permeation and metabolic control of expression in liver. 
  • Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains. 
  • Aquaporins in Saccharomyces: Characterization of a second functional water channel protein. 
  • Arachidonic acid activates Kir2.3 channels by enhancing channel-phosphatidyl-inositol 4,5-bisphosphate interactions. 
  • Arsenic trioxide uptake by human and rat aquaglyceroporins. 
  • Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes. 
  • Assembly pathway of the anastral Drosophila oocyte meiosis I spindle. 
  • Atomic distance estimates from disulfides and high-affinity metal-binding sites in a K+ channel pore. 
  • Attenuation of NMDA receptor activity and neurotoxicity by nitroxyl anion, NO-. 
  • Biochemical characterization of Leishmania major aquaglyceroporin LmAQP1: possible role in volume regulation and osmotaxis. 
  • Bradykinin and nerve growth factor release the capsaicin receptor from PtdIns(4,5)P2-mediated inhibition. 
  • CFTR regulation of epithelial sodium channel. 
  • CaMKII tethers to L-type Ca2+ channels, establishing a local and dedicated integrator of Ca2+ signals for facilitation. 
  • Calcium channel function regulated by the SH3-GK module in beta subunits. 
  • Calcium-sensitive potassium channelopathy in human epilepsy and paroxysmal movement disorder. 
  • Casein kinase I phosphorylates and destabilizes the beta-catenin degradation complex. 
  • Characterization of moesin in the sea urchin Lytechinus variegatus: redistribution to the plasma membrane following fertilization is inhibited by cytochalasin B. 
  • Characterization of neutral and cationic amino acid transport in Xenopus oocytes. 
  • Characterization of the hyperpolarization-activated chloride current in dissociated rat sympathetic neurons. 
  • Chromosome distribution, molecular motors and the claret protein. 
  • Cloning of an aquaporin homologue present in water channel containing endosomes of toad urinary bladder. 
  • Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites. 
  • Combined impact of the number of pre-ovulatory oocytes and cryopreservation on IVF outcome. 
  • Comparison between laparoscopically and ultrasonographically guided transvaginal follicular aspiration methods in an in vitro fertilization program in the same patients using the same stimulation protocol. 
  • Conclusions about the effects on fertilization of time from aspiration to incubation and blood in the aspirate depend on the use of appropriate statistical techniques. 
  • Constitutive activation of the N-methyl-D-aspartate receptor via cleft-spanning disulfide bonds. 
  • Correlation of follicular diameter with oocyte recovery and maturity at the time of transvaginal follicular aspiration. 
  • Coupling of the expressed alpha 1B-adrenergic receptor to the phospholipase C pathway in Xenopus oocytes. The role of Go. 
  • Cryopreservation: the problem of evaluation. 
  • Cytoskeletal actin gene families of Xenopus borealis and Xenopus laevis. 
  • Delayed fertilization during in vitro fertilization and embryo transfer cycles: analysis of causes and impact on overall results. 
  • Different responses to repeated applications of zingerone in behavioral studies, recordings from intact and cultured TG neurons, and from VR1 receptors. 
  • Differential expression of p63 isoforms in female reproductive organs. 
  • Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex. 
  • Dose-dependent and isoform-specific modulation of Ca2+ channels by RGK GTPases. 
  • Ectopic pregnancy rate increases with the number of retrieved oocytes in autologous in vitro fertilization with non-tubal infertility but not donor/recipient cycles: an analysis of 109,140 clinical pregnancies from the Society for Assisted Reproductive Technology registry. 
  • Effects of multiple metal binding sites on calcium and magnesium-dependent activation of BK channels. 
  • Enhancement of the ncdD microtubule motor mutant by mutants of alpha Tub67C. 
  • Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation. 
  • Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes. 
  • Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance. 
  • Expression of the mammalian system A neutral amino acid transporter in Xenopus oocytes. 
  • Fast and slow gating of sodium channels encoded by a single mRNA. 
  • Features of programmed cell death in intact Xenopus oocytes and early embryos revealed by near-infrared fluorescence and real-time monitoring. 
  • Fertilization and in vitro development of cryopreserved human prophase I oocytes. 
  • Follicular atresia associated with concurrent initiation of gonadotropin-releasing hormone agonist and follicle-stimulating hormone for oocyte recruitment. 
  • Follicular development, acquisition of mature oocytes, and pregnancy after 2 weeks of leuprolide acetate administration during the midluteal phase. 
  • Forskolin stimulation of water and cation permeability in aquaporin 1 water channels. 
  • Freezing techniques and results. 
  • Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA. 
  • Functional activity and regulation of human beta 2-adrenergic receptors expressed in Xenopus oocytes. 
  • Functional analysis of a mammalian odorant receptor subfamily. 
  • Gating currents from a delayed rectifier K+ channel with altered pore structure and function. 
  • Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1. 
  • Gonadotropin stimulation for in vitro fertilization and embryo transfer in insulin-dependent diabetics: follicular response, oocyte quality, embryo development, and follicular environment. 
  • Hamster sperm penetration of the zona pellucida: kinematic analysis and mechanical implications. 
  • High Peak Estradiol/Mature Oocyte Ratio Predicts Lower Clinical Pregnancy, Ongoing Pregnancy, and Live Birth Rates in GnRH Antagonist Intracytoplasmic Sperm Injection Cycles. 
  • High affinity copper transport protein in the lizard Podarcis sicula: molecular cloning, functional characterization and expression in somatic tissues, follicular oocytes and eggs. 
  • High-dose follicle-stimulating hormone stimulation at the onset of the menstrual cycle does not improve the in vitro fertilization outcome in low-responder patients. 
  • Human embryos derived from in vitro and in vivo matured oocytes: analysis for chromosomal abnormalities and nuclear morphology. 
  • Hyperglycosylated human chorionic gonadotropin does not increase progesterone production by luteinized granulosa cells. 
  • Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes. 
  • Improved oocyte quality is obtained with follicle stimulating hormone alone than with follicle stimulating hormone/human menopausal gonadotrophin combination. 
  • In vitro capacitation of human spermatozoa after passage through a column of cervical mucus. 
  • In vitro fertilization and embryo transfer (IVF/ET): an established and successful therapy for endometriosis. 
  • Influence of the gamma subunit and expression system on acetylcholine receptor gating. 
  • Influence of vitamin A injection before mating on oocyte development, follicular hormones, and ovulation in gilts fed high-energy diets. 
  • Inhibition of ras-induced germinal vesicle breakdown in Xenopus oocytes by rap-1B. 
  • Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase. 
  • Interaction between residues in the Mg2+-binding site regulates BK channel activation. 
  • K channel subconductance levels result from heteromeric pore conformations. 
  • KCNQ1 assembly and function is blocked by long-QT syndrome mutations that disrupt interaction with calmodulin. 
  • Localization of the activation gate for small conductance Ca2+-activated K+ channels. 
  • Maintenance of G2 arrest in the Xenopus oocyte: a role for 14-3-3-mediated inhibition of Cdc25 nuclear import. 
  • Meiosis, mitosis and microtubule motors. 
  • Meiotic chromosome distribution in Drosophila oocytes: roles of two kinesin-related proteins. 
  • Membrane localization of the kinase which phosphorylates p34cdc2 on threonine 14. 
  • Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2. 
  • Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2. 
  • Microinjection of Rap2B protein or RNA induces rearrangement of pigment granules in Xenopus oocytes. 
  • Microinjection of a 19-kDa guanine nucleotide-binding protein inhibits maturation of Xenopus oocytes. 
  • Minimal ovarian hyperstimulation for in vitro fertilization using sequential clomiphene citrate and gonadotropin with or without the addition of a gonadotropin-releasing hormone antagonist. 
  • Missense mutation (G480C) in the CFTR gene associated with protein mislocalization but normal chloride channel activity. 
  • Modulation of Xenopus oocyte-expressed phospholemman-induced ion currents by co-expression of protein kinases. 
  • Molecular biology of adrenergic receptors. 
  • Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1. 
  • Mutagenic analysis of functional domains of the mos proto-oncogene and identification of the sites important for MAPK activation and DNA binding. 
  • Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis. 
  • Ncd motor binding and transport in the spindle. 
  • Neutrophil Elastase Activates Protease-activated Receptor-2 (PAR2) and Transient Receptor Potential Vanilloid 4 (TRPV4) to Cause Inflammation and Pain. 
  • Non-functional ovarian cysts do not affect ipsilateral or contralateral ovarian performance during in-vitro fertilization. 
  • Nuclear export of mammalian PERIOD proteins. 
  • Oocyte donation. The Norfolk Program. 
  • Oocyte number as a predictor for ovarian hyperstimulation syndrome and live birth: an analysis of 256,381 in vitro fertilization cycles. 
  • Orphan transporter SLC6A18 is renal neutral amino acid transporter B0AT3. 
  • PP1 control of M phase entry exerted through 14-3-3-regulated Cdc25 dephosphorylation. 
  • PP1-mediated dephosphorylation of phosphoproteins at mitotic exit is controlled by inhibitor-1 and PP1 phosphorylation. 
  • Penetration of human spermatozoa into the human zona pellucida and the zona-free hamster egg: a study of fertile donors and infertile patients. 
  • Percutaneous epididymal sperm aspiration and intracytoplasmic sperm injection in the management of infertility due to obstructive azoospermia. 
  • Pertussis-toxin-sensitive Galpha subunits selectively bind to C-terminal domain of neuronal GIRK channels: evidence for a heterotrimeric G-protein-channel complex. 
  • Phosphorylation and nuclear translocation of a regulator of G protein signaling (RGS10). 
  • Pregnancy following transfer of ooplasm from cryopreserved-thawed donor oocytes into recipient oocytes. 
  • Prevention of the polycystic ovarian phenotype and characterization of ovulatory capacity in the estrogen receptor-alpha knockout mouse. 
  • Progesterone levels on the day of human chorionic gonadotropin do not predict pregnancy outcome from the transfer of fresh or cryopreserved embryos from the same cohort. 
  • RCC1, a regulator of mitosis, is essential for DNA replication. 
  • Reaper eliminates IAP proteins through stimulated IAP degradation and generalized translational inhibition. 
  • Regulation of biliary secretion through apical purinergic receptors in cultured rat cholangiocytes. 
  • Requirement of the prolyl isomerase Pin1 for the replication checkpoint. 
  • Restraint of apoptosis during mitosis through interdomain phosphorylation of caspase-2. 
  • Reversible inhibition of Hsp70 chaperone function by Scythe and Reaper. 
  • Role of a key cysteine residue in the gating of the acetylcholine receptor. 
  • Satellite DNA is transcribed on lampbrush chromosomes. 
  • Sequence elements required for transcriptional activity of the human myoglobin promoter in intact myocardium. 
  • Sequence- or position-specific mutations in the carboxyl-terminal FL motif of the kidney sodium bicarbonate cotransporter (NBC1) disrupt its basolateral targeting and alpha-helical structure. 
  • Significantly enhanced pregnancy rates per cycle through cryopreservation and thaw of pronuclear stage oocytes. 
  • Site-directed mutagenesis of the cytoplasmic domains of the human beta 2-adrenergic receptor. Localization of regions involved in G protein-receptor coupling. 
  • Specificity of Gbetagamma signaling depends on Galpha subunit coupling with G-protein-sensitive K(+) channels. 
  • Spindle dynamics during meiosis in Drosophila oocytes. 
  • Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters. 
  • Structural conservation of ion conduction pathways in K channels and glutamate receptors. 
  • Subunit-specific effect of the voltage sensor domain on Ca2+ sensitivity of BK channels. 
  • Superovulation with intrauterine insemination in the treatment of infertility: a possible alternative to gamete intrafallopian transfer and in vitro fertilization. 
  • Synthesis and complete processing of a high-molecular-weight precursor polypeptide to viral structural proteins in toad oocytes micro-injected with avian myeloblastosis virus ribonucleic acid [proceedings] 
  • TMEM16F forms a Ca2+-activated cation channel required for lipid scrambling in platelets during blood coagulation. 
  • Tetraethylammonium block of water flux in Aquaporin-1 channels expressed in kidney thin limbs of Henle's loop and a kidney-derived cell line. 
  • The 5'-untranslated region of the N-methyl-D-aspartate receptor NR2A subunit controls efficiency of translation. 
  • The Drosophila ncd microtubule motor protein is spindle-associated in meiotic and mitotic cells. 
  • The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. 
  • The NMDA receptor M3 segment is a conserved transduction element coupling ligand binding to channel opening. 
  • The activation mechanism of rat vanilloid receptor 1 by capsaicin involves the pore domain and differs from the activation by either acid or heat. 
  • The antibiotic azatyrosine suppresses progesterone or [Val12]p21 Ha-ras/insulin-like growth factor I-induced germinal vesicle breakdown and tyrosine phosphorylation of Xenopus mitogen-activated protein kinase in oocytes. 
  • The combination of follicle-stimulating hormone and human menopausal gonadotropin for the induction of multiple follicular maturation for in vitro fertilization. 
  • The evolution of embryonic patterning mechanisms in animals. 
  • The gonadotropin-releasing hormone agonist stimulation test--a sensitive predictor of performance in the flare-up in vitro fertilization cycle. 
  • The molecular basis for ligand specificity in a mouse olfactory receptor: a network of functionally important residues. 
  • The potassium channel MBK1 (Kv1.1) is expressed in the mouse retina. 
  • The value of basal and/or stimulated serum gonadotropin levels in prediction of stimulation response and in vitro fertilization outcome. 
  • Thermal behaviour of Anopheles stephensi in response to infection with malaria and fungal entomopathogens. 
  • Timing of oocyte retrieval in cycles with a spontaneous luteinizing hormone surge in a large in vitro fertilization program. 
  • Toxin and kinetic profile of rat brain type III sodium channels expressed in Xenopus oocytes. 
  • Transcriptome analysis of mouse stem cells and early embryos. 
  • Translation of 35S RNA from avian myeloblastosis virus into viral specific polypeptides in Xenopus oocytes. 
  • Tuning magnesium sensitivity of BK channels by mutations. 
  • Two PKC consensus sites on human acid-sensing ion channel 1b differentially regulate its function. 
  • Two forms of mitochondrial DNA ligase III are produced in Xenopus laevis oocytes. 
  • Two separate interfaces between the voltage sensor and pore are required for the function of voltage-dependent K(+) channels. 
  • Use of hMG and/or FSH for multiple follicle development. 
  • Value of suppression with a gonadotropin-releasing hormone agonist prior to gonadotropin stimulation for in vitro fertilization. 
  • Vertebrate Reproduction. 
  • Vitellogenin association and oocytic accumulation of thyroxine and 3, 5,3'-triiodothyronine in gravid Fundulus heteroclitus. 
  • WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. 
  • Wound healing: The power of the purse string. 
  • XMAP215 is a long thin molecule that does not increase microtubule stiffness. 
  • ePAD, an oocyte and early embryo-abundant peptidylarginine deiminase-like protein that localizes to egg cytoplasmic sheets. 
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  Keywords of People

  • Bennett, Vann, George B. Geller Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute
  • Carbrey, Jennifer Mehlman, Assistant Research Professor of Cell Biology, Cell Biology
  • Hogan, Brigid L. M., George Barth Geller Professor, Cell Biology
  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology
  • Wray, Gregory Allan, Professor of Biology, Duke Innovation & Entrepreneurship
  • Yang, Yiping, Professor of Medicine, Immunology