Organ Culture Techniques

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  Subject Areas on Research

  • 1,25-Dihydroxyvitamin D3 stimulates avian and mammalian cartilage growth in vitro. 
  • 4-Hydroxy-2(E)-nonenal (HNE) catabolism and formation of HNE adducts are modulated by β oxidation of fatty acids in the isolated rat heart. 
  • 8(S)-hydroxyeicosatetraenoic acid is the lipoxygenase metabolite of arachidonic acid that regulates epithelial cell migration in the rat cornea. 
  • A Small Molecule Pyrazolo[3,4-d]Pyrimidinone Inhibitor of Zipper-Interacting Protein Kinase Suppresses Calcium Sensitization of Vascular Smooth Muscle. 
  • A microfluidic system for investigation of extravascular transport and cellular uptake of drugs in tumors. 
  • A new generation organ culture arising from cross-talk between multiple primary human cell types. 
  • AAV Gene Therapy for MPS1-associated Corneal Blindness. 
  • AMH induces mesonephric cell migration in XX gonads. 
  • Adenosine 3',5'-monophosphate: a modulator of embryonic chick cartilage growth. 
  • Akt is required for Stat5 activation and mammary differentiation. 
  • Allelic exclusion of the TCR alpha-chain is an active process requiring TCR-mediated signaling and c-Cbl. 
  • Altered regulation of brain-derived neurotrophic factor protein in hippocampus following slice preparation. 
  • ApoE isoform-specific effects on LTP: blockade by oligomeric amyloid-beta1-42. 
  • Aquaporin-1 expression and conventional aqueous outflow in human eyes. 
  • Arachidonic acid stimulates mucin secretion in prairie dog gallbladder. 
  • Assessment of sex steroid action in vitro. 
  • BDNF mediates neuroprotection against oxygen-glucose deprivation by the cardiac glycoside oleandrin. 
  • BMP antagonism is required in both the node and lateral plate mesoderm for mammalian left-right axis establishment. 
  • Beta1-adrenergic receptors maintain fetal heart rate and survival. 
  • Bile salts stimulate glycoprotein release by guinea pig gallbladder in vitro. 
  • Bimatoprost, prostamide activity, and conventional drainage. 
  • Binge-pattern ethanol exposure during adolescence, but not adulthood, causes persistent changes in GABAA receptor-mediated tonic inhibition in dentate granule cells. 
  • Bmp4 and Fgf10 play opposing roles during lung bud morphogenesis. 
  • Bombesin increases fetal lung growth and maturation in utero and in organ culture. 
  • Bombesin may play a role in fetal lung growth and maturation in utero and in lung organ culture. 
  • Bombesin-like peptide and receptors in lung injury models: diverse gene expression, similar function. 
  • Bombesin-like peptide receptor gene expression, regulation, and function in fetal murine lung. 
  • Bombesin-like peptides and receptors in normal fetal baboon lung: roles in lung growth and maturation. 
  • Bombyxin is a growth factor for wing imaginal disks in Lepidoptera. 
  • Bone morphogenetic protein 4 regulates the budding site and elongation of the mouse ureter. 
  • CD10/neutral endopeptidase 24.11 in developing human fetal lung. Patterns of expression and modulation of peptide-mediated proliferation. 
  • Calcitonin stimulates growth and maturation of embryonic chick pelvic cartilage in vitro. 
  • Calcium-binding proteins define interneurons in HVC of the zebra finch (Taeniopygia guttata). 
  • Catecholamines act via a beta-adrenergic receptor to maintain fetal heart rate and survival. 
  • Centrifugal migration of mesenchymal cells in embryonic lung. 
  • Characterization of a thymus-tropic HIV-1 isolate from a rapid progressor: role of the envelope. 
  • Characterization of cultured thymus tissue used for transplantation with emphasis on promiscuous expression of thyroid tissue-specific genes. 
  • Cholecystokinin action on layer 6b neurons in somatosensory cortex. 
  • Comparison of Cartilage Mechanical Properties Measured During Creep and Recovery. 
  • Conservation and regeneration of transgenic lines of swine by semen cryopreservation and artificial insemination. 
  • Constitutive hedgehog signaling in chondrosarcoma up-regulates tumor cell proliferation. 
  • Continuous human glioma-derived cell lines UC-11MG and UC-302MG. Morphologic, immunocytochemical and chromosomal characterization. 
  • Contributions of mossy fiber and CA1 pyramidal cell sprouting to dentate granule cell hyperexcitability in kainic acid-treated hippocampal slice cultures. 
  • Control of embryonic lung branching morphogenesis by the Rho activator, cytotoxic necrotizing factor 1. 
  • Control of liver and brain aromatic amino-acid metabolism by phenylalanine hydroxylase. 
  • Convergence of presenilin- and tau-mediated pathways on axonal trafficking and neuronal function. 
  • Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus. 
  • Cultured thymus tissue implantation promotes donor-specific tolerance to allogeneic heart transplants. 
  • Cyclooxygenase-1 and -2 knockout mice demonstrate increased cardiac ischemia/reperfusion injury but are protected by acute preconditioning. 
  • Cytokine mechanisms of central sensitization: distinct and overlapping role of interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha in regulating synaptic and neuronal activity in the superficial spinal cord. 
  • Deconstructing the genesis of animal form. 
  • Degradation of proteoglycan in articular cartilage. 
  • Deltex1 redirects lymphoid progenitors to the B cell lineage by antagonizing Notch1. 
  • Desert Hedgehog/Patched 1 signaling specifies fetal Leydig cell fate in testis organogenesis. 
  • Developmental onset of enduring long-term potentiation in mouse hippocampus. 
  • Differences in O2 availability resolve the apparent discrepancies in metabolic intrinsic optical signals in vivo and in vitro. 
  • Differences in Reperfusion-Induced Mitochondrial Oxidative Stress and Cell Death Between Hippocampal CA1 and CA3 Subfields Are Due to the Mitochondrial Thioredoxin System. 
  • Differential expression of plasminogen activators and their inhibitors in an organotypic skin coculture system. 
  • Direct effects of corticotropin-releasing hormone and thyrotropin-releasing hormone on fetal lung explants. 
  • Distinct timing in the activity of cannabinoid-sensitive and cannabinoid-insensitive basket cells. 
  • Distributing a fixed amount of cyclic loading to tendon explants over longer periods induces greater cellular and mechanical responses. 
  • Divergent vascular mechanisms downstream of Sry establish the arterial system in the XY gonad. 
  • Dopaminergic control of corticostriatal long-term synaptic depression in medium spiny neurons is mediated by cholinergic interneurons. 
  • Dual role of the exocyst in AMPA receptor targeting and insertion into the postsynaptic membrane. 
  • Dynamic culture yields engineered myocardium with near-adult functional output. 
  • Effect of inhibitors of arachidonic acid metabolism on corneal reepithelialization in the rat. 
  • Effect of lipophilicity of Mn (III) ortho N-alkylpyridyl- and diortho N, N'-diethylimidazolylporphyrins in two in-vitro models of oxygen and glucose deprivation-induced neuronal death. 
  • Effect of perillic acid, a putative isoprenylation inhibitor, on the cultured rat lens. 
  • Effect of protein kinase C inhibitors and activators on corneal re-epithelialization in the rat. 
  • Effects of Copper and Cross-Linking on the Extracellular Matrix of Tissue-Engineered Arteries. 
  • Effects of cholesterol-lowering statins on the aqueous humor outflow pathway. 
  • Effects of copper and cross-linking on the extracellular matrix of tissue-engineered arteries. 
  • Effects of cyclic intraocular pressure on conventional outflow facility. 
  • Effects of estrogen on very low density lipoproteins (VLDL) synthesis in avian liver slices in vitro: lack of correlation with nuclear estrogen receptors. 
  • Effects of pharmacologic inhibition of protein geranylgeranyltransferase type I on aqueous humor outflow through the trabecular meshwork. 
  • Effects of pulsatile perfusion on human saphenous vein vasoreactivity: a preliminary report. 
  • Embryonic chick cartilage produces its own somatomedin-like peptide to stimulate cartilage growth in vitro. 
  • Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. 
  • Endothelial deletion of murine Jag1 leads to valve calcification and congenital heart defects associated with Alagille syndrome. 
  • Estrogen and progesterone production by developing porcine follicles in vitro: evidence for estrogen formation by theca. 
  • Experimental and numerical studies of adenovirus delivery to outflow tissues of perfused human anterior segments. 
  • Explant culture of adult zebrafish hearts for epicardial regeneration studies. 
  • Expression analysis of the matrix GLA protein and VE-cadherin gene promoters in the outflow pathway. 
  • Expression of dominant negative Rho-binding domain of Rho-kinase in organ cultured human eye anterior segments increases aqueous humor outflow. 
  • Extracellular-signal regulated kinase 1-dependent metabotropic glutamate receptor 5-induced long-term depression in the bed nucleus of the stria terminalis is disrupted by cocaine administration. 
  • FGF9 promotes survival of germ cells in the fetal testis. 
  • Fasciated nerve-muscle explants for in vitro comparison of magnetic and electrical neuromuscular stimulation. 
  • Fetal alveolar epithelial cells contain [D-Ala(2)]-deltorphin I-like immunoreactivity: delta- and mu-opiate receptors mediate opposite effects in developing lung. 
  • First-trimester human chorionic villi express both immunoregulatory and inflammatory cytokines: a role for interleukin-10 in regulating the cytokine network of pregnancy. 
  • Flow perfusion maintains ex vivo bone viability: a novel model for bone biology research. 
  • Four-dimensional analysis of vascularization during primary development of an organ, the gonad. 
  • Function of microglia in organotypic slice cultures. 
  • GABA signaling promotes synapse elimination and axon pruning in developing cortical inhibitory interneurons. 
  • GAD67-mediated GABA synthesis and signaling regulate inhibitory synaptic innervation in the visual cortex. 
  • Glutamate acting at NMDA receptors stimulates embryonic cortical neuronal migration. 
  • Glutamate receptor involvement in dentate granule cell epileptiform activity evoked by mossy fiber stimulation. 
  • High-fidelity optical excitation of cortico-cortical projections at physiological frequencies. 
  • Histopathologic assessment of cultured human thymus. 
  • Hormonal and metabolic regulation of human chondrosarcoma in vitro. 
  • Hormonal stimulation of avian embryonic cartilage growth in vitro: histologic and ultrastructural features. 
  • Human Placental Syncytiotrophoblasts Restrict Toxoplasma gondii Attachment and Replication and Respond to Infection by Producing Immunomodulatory Chemokines. 
  • Human epithelial cell expression of an Epstein-Barr virus receptor. 
  • Hydrocortisone inhibits mucin secretion from guinea pig gallbladder. 
  • Identification of a peripheral nerve neurite growth-promoting activity by development and use of an in vitro bioassay. 
  • Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon. 
  • In vitro neurogenesis by neuronal precursor cells derived from the adult songbird brain. 
  • In vitro stimulation of alkaline phosphatase activity in immature embryonic chick pelvic cartilage by adenosine 3'5'-monophosphate. 
  • In vitro studies of acantholysis of keratinocytes induced by pemphigus antibody. 
  • Inhibition of fetal thymic caspases abrogates the consequences of adenosine deaminase deficiency. 
  • Insufficient luteinizing hormone-induced intracellular signaling disrupts ovulation in preovulatory follicles lacking estrogen receptor-{beta}. 
  • Interneurons of the dentate-hilus border of the rat dentate gyrus: morphological and electrophysiological heterogeneity. 
  • Investigations into the loss of glutathione from lenses in organ culture. 
  • Involvement of R-cadherin in the early stage of glomerulogenesis. 
  • Involvement of urokinase-type plasminogen activator in acantholysis induced by pemphigus IgG. 
  • Ionotropic and metabotropic receptors, protein kinase A, protein kinase C, and Src contribute to C-fiber-induced ERK activation and cAMP response element-binding protein phosphorylation in dorsal horn neurons, leading to central sensitization. 
  • Is endogenous D-serine in the rostral anterior cingulate cortex necessary for pain-related negative affect? 
  • Isoflurane-induced neuronal degeneration: an evaluation in organotypic hippocampal slice cultures. 
  • Isogenic normal basal and luminal mammary epithelial isolated by a novel method show a differential response to ionizing radiation. 
  • Isolated Primary Blast Inhibits Long-Term Potentiation in Organotypic Hippocampal Slice Cultures. 
  • Isolated primary blast alters neuronal function with minimal cell death in organotypic hippocampal slice cultures. 
  • Kinetics and glial fibrillary acidic (GFA) protein production in a transplantable human giant cell glioblastoma (D-212 MG) of near haploid karyotype maintained in an organ culture system. An immunohistochemistry study. 
  • LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development. 
  • LTP in the mouse nucleus accumbens is developmentally regulated. 
  • Lactate uptake contributes to the NAD(P)H biphasic response and tissue oxygen response during synaptic stimulation in area CA1 of rat hippocampal slices. 
  • Lambert-Eaton sera reduce low-voltage and high-voltage activated Ca2+ currents in murine dorsal root ganglion neurons. 
  • Leukocyte inflammatory response in a rat urinary bladder regeneration model using porcine small intestinal submucosa scaffold. 
  • Ligand-specific selection of MHC class II-restricted thymocytes in fetal thymic organ culture. 
  • Linkage of the CCR5 Delta 32 mutation with a functional polymorphism of CD45RA. 
  • Long-term plasticity mediated by mGluR1 at a retinal reciprocal synapse. 
  • MTOR-independent induction of autophagy in trabecular meshwork cells subjected to biaxial stretch. 
  • Male-specific cell migration into the developing gonad. 
  • Mast cell stabilization improves survival by preventing apoptosis in sepsis. 
  • McCarey-Kaufman (MK) organ culture and MK medium-shifted corneas. 
  • Mediators, initiating the inflammatory response, released in organ culture by full-thickness human skin explants exposed to the irritant, sulfur mustard. 
  • Membrane properties underlying spontaneous activity of denervated muscle fibres. 
  • Mesonephric cell migration induces testis cord formation and Sertoli cell differentiation in the mammalian gonad. 
  • Metabolism of 1,2-dimethylhydrazine by cultured human colon. 
  • Metabolism of aflatoxin B1, benzo[a]pyrene, and 1,2-dimethylhydrazine by cultured rat and human colon. 
  • Metabolites from apoptotic thymocytes inhibit thymopoiesis in adenosine deaminase-deficient fetal thymic organ cultures. 
  • Migration of mesonephric cells into the mammalian gonad depends on Sry. 
  • Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age. 
  • Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity. 
  • Modulation of NMDA and AMPA-mediated synaptic transmission by CB1 receptors in frontal cortical pyramidal cells. 
  • Modulation of the GABA(A)-gated chloride channel by reactive oxygen species. 
  • Molecular mechanisms of pathogenesis. 
  • Munc13-1 is required for presynaptic long-term potentiation. 
  • Mutual regulation of Src family kinases and the neurotrophin receptor TrkB. 
  • NADH hyperoxidation correlates with enhanced susceptibility of aged rats to hypoxia. 
  • NOV (CCN3) regulation in the growth plate and CCN family member expression in cartilage neoplasia. 
  • Neointimal inflammation and adventitial angiogenesis correlate with severity of cardiac allograft vasculopathy in pediatric recipients. 
  • Neural cell adhesion molecule-mediated Fyn activation promotes GABAergic synapse maturation in postnatal mouse cortex. 
  • Neural induction and patterning in the mouse in the absence of the node and its derivatives. 
  • Neurite outgrowth from progeny of epidermal growth factor-responsive hippocampal stem cells is significantly less robust than from fetal hippocampal cells following grafting onto organotypic hippocampal slice cultures: effect of brain-derived neurotrophic factor. 
  • Neuropeptide Y regulates recurrent mossy fiber synaptic transmission less effectively in mice than in rats: Correlation with Y2 receptor plasticity. 
  • Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7. 
  • Nicotine evokes cell death in embryonic rat brain during neurulation. 
  • Nitric oxide as a mediator of relaxation of the corpus cavernosum in response to nonadrenergic, noncholinergic neurotransmission. 
  • Nitric oxide modulates branching morphogenesis in fetal rat lung explants. 
  • Novel in vitro gene transfer method for study of local modulators in vascular smooth muscle cells. 
  • Novel molecular insights into RhoA GTPase-induced resistance to aqueous humor outflow through the trabecular meshwork. 
  • Optical imaging of intracellular chloride in living brain slices. 
  • Osteoblasts express the inflammatory cytokine interleukin-6 in a murine model of Staphylococcus aureus osteomyelitis and infected human bone tissue. 
  • Parathyroid hormone stimulates growth of embryonic chick pelvic cartilage in vitro. 
  • Pericyte-like location of GFP-tagged melanoma cells: ex vivo and in vivo studies of extravascular migratory metastasis. 
  • Pharmacological regulation of outflow resistance distal to Schlemm's canal. 
  • Phenylalanine hydroxylase activity in foetal hepatic organ culture. 
  • Plasticity of both excitatory and inhibitory synapses is associated with seizures induced by removal of chronic blockade of activity in cultured hippocampus. 
  • Postsynaptic response to stimulation of the Schaffer collaterals with properties similar to those of synaptosomal aspartate release. 
  • Prenatal choline supplementation alters hippocampal N-methyl-D-aspartate receptor-mediated neurotransmission in adult rats. 
  • Prevention of fumonisin B1-induced neural tube defects by folic acid. 
  • Primary Blast Exposure Increases Hippocampal Vulnerability to Subsequent Exposure: Reducing Long-Term Potentiation. 
  • Proline-induced potentiation of glutamate transmission. 
  • Properties of the pathways from the lateral amygdal nucleus to basolateral nucleus and amygdalostriatal transition area. 
  • Protein kinase C and Ca2+ activation of mucin secretion in airway goblet cells. 
  • Protein phosphatases mediate depotentiation induced by high-intensity theta-burst stimulation. 
  • Punicalagin inhibits neuroinflammation in LPS-activated rat primary microglia. 
  • Quantitative analysis of angiogenesis and growth of bone: effect of indomethacin exposure in a combined in vitro-in vivo approach. 
  • Rate of change in central corneal thickness: a viability indicator for conventional drainage tissues in organ culture. 
  • Recurrent mossy fiber pathway in rat dentate gyrus: synaptic currents evoked in presence and absence of seizure-induced growth. 
  • Regenerative scaffold electrodes for peripheral nerve interfacing. 
  • Regulation of matrix turnover in meniscal explants: role of mechanical stress, interleukin-1, and nitric oxide. 
  • Role for ETS domain transcription factors Pea3/Erm in mouse lung development. 
  • Role of NMDA, non-NMDA, and GABA receptors in signal propagation in the amygdala formation. 
  • Role of small GTP-binding proteins in lovastatin-induced cataracts. 
  • SHP-2 is required for the maintenance of cardiac progenitors. 
  • Schizophrenia-like GABAergic gene expression deficits in cerebellar Golgi cells from rats chronically exposed to low-dose phencyclidine. 
  • Seizures, cell death, and mossy fiber sprouting in kainic acid-treated organotypic hippocampal cultures. 
  • Sertoli cell ablation and replacement of the spermatogonial niche in mouse. 
  • Shortening increases spontaneous contractility in myometrium from pregnant women at term. 
  • Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons. 
  • Similar propagation of SD and hypoxic SD-like depolarization in rat hippocampus recorded optically and electrically. 
  • Small interfering RNA-mediated suppression of proislet amyloid polypeptide expression inhibits islet amyloid formation and enhances survival of human islets in culture. 
  • Staging worms for next-generation analysis. 
  • Steroidogenesis in porcine atretic follicles: loss of aromatase activity in isolated granulosa and theca. 
  • Studying the influence of angiogenesis in in vitro cancer model systems. 
  • Subcellular domain-restricted GABAergic innervation in primary visual cortex in the absence of sensory and thalamic inputs. 
  • Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus. 
  • Superoxide production, risk factors, and endothelium-dependent relaxations in human internal mammary arteries. 
  • Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. 
  • Synergistic roles of neuregulin-1 and insulin-like growth factor-I in activation of the phosphatidylinositol 3-kinase pathway and cardiac chamber morphogenesis. 
  • Targeted gene transfer to Schlemm's canal by retroperfusion. 
  • Tau protein is required for amyloid {beta}-induced impairment of hippocampal long-term potentiation. 
  • The 25-kilodalton insulin-like growth factor (IGF)-binding protein inhibits both basal and IGF-I-mediated growth of chick embryo pelvic cartilage in vitro. 
  • The effect of contractile activity on fibrillation and extrajunctional acetylcholine-sensitivity in rat muscle maintained in organ culture. 
  • The effect of irrigation solution at different temperatures on articular cartilage metabolism. 
  • The effects of chloroquine on chick skeletal muscle in vitro. 
  • The human thymic microenvironment during organ culture. 
  • The role of WNT10B in normal prostate gland development and prostate cancer. 
  • The role of protein kinase C in modulation of aqueous humor outflow facility. 
  • The role of protein synthesis in striatal long-term depression. 
  • The role of the forkhead transcription factor, Foxc1, in the development of the mouse lacrimal gland. 
  • The suppression of testis-brain RNA binding protein and kinesin heavy chain disrupts mRNA sorting in dendrites. 
  • Three-dimensional (3D) tetra-culture brain on chip platform for organophosphate toxicity screening. 
  • Thymus transplantation for complete DiGeorge syndrome: European experience. 
  • Tissue interactions pattern the mesenchyme of the embryonic mouse lung. 
  • Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics. 
  • Triiodothyronine stimulates cartilage growth and maturation by different mechanisms. 
  • Utilization of the chick chorioallantoic membrane for in vitro growth of the embryonic murine kidney. 
  • Vanadyl sulfate inhibits NO production via threonine phosphorylation of eNOS. 
  • Yolk-sac-derived macrophages regulate fetal testis vascularization and morphogenesis. 
  • miR-181a is an intrinsic modulator of T cell sensitivity and selection. 
  • p38α mitogen-activated protein kinase depletion and repression of signal transduction to translation machinery by miR-124 and -128 in neurons. 
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  Keywords of People

  • Allori, Alexander C, Associate Professor of Surgery, Surgery, Plastic, Maxillofacial, and Oral Surgery
  • Ciofani, Maria, Associate Professor of Integrative Immunobiology, Cell Biology
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology
  • Preminger, Glenn Michael, James F. Glenn, M.D. Distinguished Professor of Urology, Surgery, Urology
  • Stinnett, Sandra Sue, Associate Professor of Biostatistics & Bioinformatics, Biostatistics & Bioinformatics
  • Sunday, Mary Elizabeth Anne, Professor of Pathology, Cell Biology