Phosphopeptides

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  Subject Areas on Research

  • A G-protein beta gamma-subunit-responsive phosphoinositide 3-kinase activity in human platelet cytosol. 
  • A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. 
  • Adducin regulation. Definition of the calmodulin-binding domain and sites of phosphorylation by protein kinases A and C. 
  • Analysis of sites phosphorylated on acetyl-CoA carboxylase in response to insulin in isolated adipocytes. Comparison with sites phosphorylated by casein kinase-2 and the calmodulin-dependent multiprotein kinase. 
  • Automated, reproducible, titania-based phosphopeptide enrichment strategy for label-free quantitative phosphoproteomics. 
  • BiPS, a photocleavable, isotopically coded, fluorescent cross-linker for structural proteomics. 
  • Blocking Interleukin (IL)4- and IL13-Mediated Phosphorylation of STAT6 (Tyr641) Decreases M2 Polarization of Macrophages and Protects Against Macrophage-Mediated Radioresistance of Inflammatory Breast Cancer. 
  • Calmodulin-dependent multifunctional protein kinase. Evidence for isoenzyme forms in mammalian tissues. 
  • Capture of phosphopeptides using alpha-zirconium phosphate nanoplatelets. 
  • Casein kinase I phosphorylates and destabilizes the beta-catenin degradation complex. 
  • Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line. 
  • GPCR-mediated β-arrestin activation deconvoluted with single-molecule precision. 
  • Getting a grip on the CTD of Pol II. 
  • HER-2/c-erbB2 is phosphorylated by calmodulin-dependent protein kinase II on a single site in the cytoplasmic tail at threonine-1172. 
  • Identification of substrate specificity determinants for the cell cycle-regulated NIMA protein kinase. 
  • Inhibition of the Staphylococcus aureus sortase transpeptidase SrtA by phosphinic peptidomimetics. 
  • Insulin and phorbol ester stimulate phosphorylation of acetyl-CoA carboxylase at similar sites in isolated adipocytes. Lack of correspondence with sites phosphorylated on the purified enzyme by protein kinase C. 
  • Investigation of phosphoprotein signatures of archived prostate cancer tissue specimens via proteomic analysis. 
  • Longitudinal study of differential protein expression in an Alzheimer's mouse model lacking inducible nitric oxide synthase. 
  • Mass Spectrometry-Based Proteomics for Analysis of Hydrophilic Phosphopeptides. 
  • Phosphoproteomic profiling of human myocardial tissues distinguishes ischemic from non-ischemic end stage heart failure. 
  • Phosphorylation of c-Abl by protein kinase Pak2 regulates differential binding of ABI2 and CRK. 
  • Phosphorylation specificities of protein kinase C isozymes for bovine cardiac troponin I and troponin T and sites within these proteins and regulation of myofilament properties. 
  • Phosphorylation-dependent metal binding by alpha-synuclein peptide fragments. 
  • Proteomic dissection of LPS-inducible, PHF8-dependent secretome reveals novel roles of PHF8 in TLR4-induced acute inflammation and T cell proliferation. 
  • Quantitative and selective fluorophore labeling of phosphoserine on peptides and proteins: characterization at the attomole level by capillary electrophoresis and laser-induced fluorescence. 
  • Quantitative label-free phosphoproteomics strategy for multifaceted experimental designs. 
  • Regulation of the association of adducin with actin filaments by Rho-associated kinase (Rho-kinase) and myosin phosphatase. 
  • Structural studies of phosphorylation-dependent interactions between the V2R receptor and arrestin-2. 
  • Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide. 
  • Tandem Mass Tag Labeling Facilitates Reversed-Phase Liquid Chromatography-Mass Spectrometry Analysis of Hydrophilic Phosphopeptides. 
  • cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Hirschey, Matthew, Associate Professor of Medicine, Cell Biology
  • Milano, Carmelo Alessio, Joseph W. and Dorothy W. Beard Distinguished Professor of Experimental Surgery, Surgery, Cardiovascular and Thoracic Surgery