Plasmids

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  Subject Areas on Research

  • 'Interactive' recognition in EcoRI restriction enzyme-DNA complex. 
  • 2'-deoxy-5-azacytidine increases binding of cisplatin to DNA by a mechanism independent of DNA hypomethylation. 
  • 2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae. 
  • 5-Azacytidine induced methyltransferase-DNA adducts block DNA replication in vivo. 
  • A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time. 
  • A T cell-specific transcriptional enhancer within the human T cell receptor delta locus. 
  • A cAMP response element in the beta 2-adrenergic receptor gene confers transcriptional autoregulation by cAMP. 
  • A case-control study of nosocomial ampicillin-resistant enterococcal infection and colonization at a university hospital. 
  • A centralized gene-based HIV-1 vaccine elicits broad cross-clade cellular immune responses in rhesus monkeys. 
  • A cloned calmodulin structural gene probe is complementary to DNA sequences from diverse species. 
  • A defective phage system reveals bacteriophage T4 replication origins that coincide with recombination hot spots. 
  • A functional analysis of the spacer of V(D)J recombination signal sequences. 
  • A functional variant (-1304T>G) in the MKK4 promoter contributes to a decreased risk of lung cancer by increasing the promoter activity. 
  • A large U3 deletion causes increased in vivo expression from a nonintegrating lentiviral vector. 
  • A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. 
  • A method for analysis of gene expression patterns. 
  • A modified host-cell reactivation assay to measure repair of alkylating DNA damage for assessing risk of lung adenocarcinoma. 
  • A molecular basis for inositol polyphosphate synthesis in Drosophila melanogaster. 
  • A novel functional variant (-842G>C) in the PIN1 promoter contributes to decreased risk of squamous cell carcinoma of the head and neck by diminishing the promoter activity. 
  • A novel structural form of the 2 micron plasmid of the yeast Saccharomyces cerevisiae. 
  • A novel ubiquitin fusion system bypasses the mitochondria and generates biologically active Smac/DIABLO. 
  • A novel, highly regulated, rapidly inducible system for the expression of chicken progesterone receptor, cPRA, in Saccharomyces cerevisiae. 
  • A pressure-mediated nonviral method for efficient arterial gene and oligonucleotide transfer. 
  • A relocated technique of atomic force microscopy (AFM) samples and its application in molecular biology. 
  • A serum-resistant cytofectin for cellular delivery of antisense oligodeoxynucleotides and plasmid DNA. 
  • A statistical framework for determination of minimal plasmid copy number required for transgene expression in mammalian cells. 
  • A synthetic Escherichia coli predator-prey ecosystem. 
  • A system of transposon mutagenesis for bacteriophage T4. 
  • A three-dimensional ParF meshwork assembles through the nucleoid to mediate plasmid segregation. 
  • A truncated isoform of c-Mpl with an essential C-terminal peptide targets the full-length receptor for degradation. 
  • A unique role of the DNA fragmentation factor in maintaining genomic stability. 
  • A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics. 
  • A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene. 
  • AMPK and substrate availability regulate creatine transport in cultured cardiomyocytes. 
  • ARF1 regulates Nef-induced CD4 degradation. 
  • ATP hydrolysis by a CFTR domain: pharmacology and effects of G551D mutation. 
  • Abh and AbrB control of Bacillus subtilis antimicrobial gene expression. 
  • Acetylation of mitochondrial proteins. 
  • Activation and repression of mammalian gene expression by the c-myc protein. 
  • Activation of MEF2 by muscle activity is mediated through a calcineurin-dependent pathway. 
  • Activation of a phosphotyrosine phosphatase by tyrosine phosphorylation. 
  • Active immunization with tumor cells transduced by a novel AAV plasmid-based gene delivery system. 
  • Active immunotherapy of pancreatic cancer with tumor cells genetically engineered to secrete multiple cytokines. 
  • Active immunotherapy with transiently transfected cytokine-secreting tumor cells inhibits breast cancer metastases in tumor-bearing animals. 
  • Aerobic glycolysis suppresses p53 activity to provide selective protection from apoptosis upon loss of growth signals or inhibition of BCR-Abl. 
  • Agonist-dependent recruitment of phosphoinositide 3-kinase to the membrane by beta-adrenergic receptor kinase 1. A role in receptor sequestration. 
  • Allelic and ectopic interactions in recombination-defective yeast strains. 
  • Allelic and ectopic recombination between Ty elements in yeast. 
  • Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. 
  • Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming. 
  • Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. 
  • An Enhanced Synthetic Multiclade DNA Prime Induces Improved Cross-Clade-Reactive Functional Antibodies when Combined with an Adjuvanted Protein Boost in Nonhuman Primates. 
  • An Improved Protocol for the Production of Lentiviral Vectors. 
  • An adenovirus vector for efficient RNA interference-mediated suppression of target genes in insulinoma cells and pancreatic islets of langerhans. 
  • An antitumor drug-induced topoisomerase cleavage complex blocks a bacteriophage T4 replication fork in vivo. 
  • An efficient targeted radiotherapy/gene therapy strategy utilising human telomerase promoters and radioastatine and harnessing radiation-mediated bystander effects. 
  • An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb ischemia. 
  • An examination of adaptive reversion in Saccharomyces cerevisiae. 
  • Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae. 
  • Analysis of the individual regulatory components of the IncFII plasmid replication control system. 
  • Androgens amplify beta-adrenergic and FSH stimulation of granulosa cells. 
  • Angiotensin II type 2 receptor mediates vascular smooth muscle cell apoptosis and antagonizes angiotensin II type 1 receptor action: an in vitro gene transfer study. 
  • AnkB, a periplasmic ankyrin-like protein in Pseudomonas aeruginosa, is required for optimal catalase B (KatB) activity and resistance to hydrogen peroxide. 
  • Antagonism of catecholamine receptor signaling by expression of cytoplasmic domains of the receptors. 
  • Antibiotics as a selective driver for conjugation dynamics. 
  • Antibody and cellular responses to HIV vaccine regimens with DNA plasmid as compared with ALVAC priming: An analysis of two randomized controlled trials. 
  • Apoptotic nuclease assays. 
  • Aquaglyceroporin AQP9: solute permeation and metabolic control of expression in liver. 
  • Aromatic hydrocarbon receptor polymorphism: development of new methods to correlate genotype with phenotype. 
  • Arrest of replication by mammalian DNA polymerases alpha and beta caused by chromium-DNA lesions. 
  • Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9. 
  • Association of a functional tandem repeats in the downstream of human telomerase gene and lung cancer. 
  • Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability. 
  • Atomic force microscope investigation of large-circle DNA molecules. 
  • Augmentation of immune responses to HIV-1 and simian immunodeficiency virus DNA vaccines by IL-2/Ig plasmid administration in rhesus monkeys. 
  • BCR sequences essential for transformation by the BCR-ABL oncogene bind to the ABL SH2 regulatory domain in a non-phosphotyrosine-dependent manner. 
  • Bacterial expression and characterization of proteins derived from the chicken calmodulin cDNA and a calmodulin processed gene. 
  • Bacterial expression vectors for calmodulin. 
  • Bacterial plasmid partition machinery: a minimalist approach to survival. 
  • Bacterial surface association of heat-labile enterotoxin through lipopolysaccharide after secretion via the general secretory pathway. 
  • Bacteriophage T4 proteins replicate plasmids with a preformed R loop at the T4 ori(uvsY) replication origin in vitro. 
  • Baculovirus expression of functional P210 BCR-ABL oncogene product. 
  • Barriers to productive transfection of trabecular meshwork cells. 
  • Base modifications in plasmid DNA caused by potassium permanganate. 
  • Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. 
  • Beta-arrestin scaffolding of phosphatidylinositol 4-phosphate 5-kinase Ialpha promotes agonist-stimulated sequestration of the beta2-adrenergic receptor. 
  • Bioluminescence imaging to track bacterial dissemination of Yersinia pestis using different routes of infection in mice. 
  • Biomaterial-mediated retroviral gene transfer using self-assembled monolayers. 
  • Bleomycin induces the hsp 70 heat shock promoter in cultured cells. 
  • COMMD proteins, a novel family of structural and functional homologs of MURR1. 
  • Calmodulin activation of target enzymes. Consequences of deletions in the central helix. 
  • Calmodulin as a regulator of cell growth and gene expression. 
  • Calmodulin mediates Ca2+ sensitivity of sodium channels. 
  • Can Ceftazidime-Avibactam and Aztreonam Overcome β-Lactam Resistance Conferred by Metallo-β-Lactamases in Enterobacteriaceae? 
  • Cassettes of the f1 intergenic region. 
  • Catalytic properties of the HhaII restriction endonuclease. 
  • Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity. 
  • Characterization and expression of the unique calmodulin gene of Aspergillus nidulans. 
  • Characterization of a defective phage system for the analysis of bacteriophage T4 DNA replication origins. 
  • Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase. 
  • Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrome P-450 reductase. 
  • Characterization of the trans-activation properties of equine herpesvirus 1 EICP0 protein. 
  • Characterization of two types of yeast ribosomal DNA genes. 
  • Chemokine-adjuvanted electroporated DNA vaccine induces substantial protection from simian immunodeficiency virus vaginal challenge. 
  • Chicken calmodulin genes. A species comparison of cDNA sequences and isolation of a genomic clone. 
  • Chicken calmodulin promoter activity in proliferating and differentiated cells. 
  • Chimeric Sindbis/eastern equine encephalitis vaccine candidates are highly attenuated and immunogenic in mice. 
  • Chimeric calmodulin-cardiac troponin C proteins differentially activate calmodulin target enzymes. 
  • Chromatin conformation of yeast centromeres 
  • Chromosomal organization of rRNA operons in Bacillus subtilis. 
  • Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. 
  • Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific. 
  • Circular ribozymes generated in Escherichia coli using group I self-splicing permuted intron-exon sequences. 
  • Circularizing ribozymes and decoy-competitors by autocatalytic splicing in vitro and in vivo. 
  • Cloning and expression of the yeast plasma membrane ATPase in Escherichia coli. 
  • Cloning of the terminal loop of vaccinia virus DNA. 
  • Coadministration of IL-12 or IL-10 expression cassettes drives immune responses toward a Th1 phenotype. 
  • Comparative ability of plasmid IL-12 and IL-15 to enhance cellular and humoral immune responses elicited by a SIVgag plasmid DNA vaccine and alter disease progression following SHIV(89.6P) challenge in rhesus macaques. 
  • Comparative immunogenicity in rhesus monkeys of multi-protein HIV-1 (CRF02_AG) DNA/MVA vaccines expressing mature and immature VLPs. 
  • Comparison of multiple vaccine vectors in a single heterologous prime-boost trial. 
  • Complex genetic interactions in a quantitative trait locus. 
  • Conditional dicentric chromosomes in yeast. 
  • Conditionally replicating lentiviral-hybrid episomal vectors for suicide gene therapy. 
  • Conformational studies of the nucleic acid binding sites for Xenopus transcription factor IIIA. 
  • Conservation of structure and location of Rhizobium meliloti and Klebsiella pneumoniae nifB genes. 
  • Constitutive proteasome-mediated turnover of Bfl-1/A1 and its processing in response to TNF receptor activation in FL5.12 pro-B cells convert it into a prodeath factor. 
  • Construction and phenotypic characterization of HIV type 1 functional envelope clones of subtypes G and F. 
  • Context dependence of meiotic recombination hotspots in yeast: the relationship between recombination activity of a reporter construct and base composition. 
  • Context-specific effects of fibulin-5 (DANCE/EVEC) on cell proliferation, motility, and invasion. Fibulin-5 is induced by transforming growth factor-beta and affects protein kinase cascades. 
  • Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. 
  • Copper ion-mediated modification of bases in DNA in vitro by benzoyl peroxide. 
  • Copper-dependent site-specific mutagenesis by benzoyl peroxide in the supF gene of the mutation reporter plasmid pS189. 
  • Copy number variation is associated with gene expression change in archaea. 
  • Correction of dystrophin expression in cells from Duchenne muscular dystrophy patients through genomic excision of exon 51 by zinc finger nucleases. 
  • Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue. 
  • Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfection. 
  • Creation of an active estrogen-responsive element by a single base change in the flanking sequence of a cellular oncogene: a possible mechanism for hormonal carcinogenesis? 
  • Cross-clade inhibition of HIV-1 replication and cytopathology by using RNase P-associated external guide sequences. 
  • Cross-dressing the virion: the transcapsidation of adeno-associated virus serotypes functionally defines subgroups. 
  • Crystal structure of the ternary complex of a NaV C-terminal domain, a fibroblast growth factor homologous factor, and calmodulin. 
  • Crystalline arrays of the Escherichia coli sn-glycerol-3-phosphate acyltransferase, an integral membrane protein. 
  • Curing Saccharomyces cerevisiae of the 2 micron plasmid by targeted DNA damage. 
  • Current Progress in Electrotransfection as a Nonviral Method for Gene Delivery. 
  • Cutting of M13mp7 phage DNA and excision of cloned single-stranded sequences by restriction endonucleases. 
  • Cyclic AMP mediated GSTP1 gene activation in tumor cells involves the interaction of activated CREB-1 with the GSTP1 CRE: a novel mechanism of cellular GSTP1 gene regulation. 
  • Cyclic AMP-dependent protein kinase catalytic subunits have divergent roles in virulence factor production in two varieties of the fungal pathogen Cryptococcus neoformans. 
  • Cyclophilin A is localized to the nucleus and controls meiosis in Saccharomyces cerevisiae. 
  • Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae. 
  • DNA Priming Increases Frequency of T-Cell Responses to a Vesicular Stomatitis Virus HIV Vaccine with Specific Enhancement of CD8+ T-Cell Responses by Interleukin-12 Plasmid DNA. 
  • DNA bending near the replication origin of IncFII plasmid NR1. 
  • DNA delivery from hyaluronic acid-collagen hydrogels via a substrate-mediated approach 
  • DNA determinants important in sequence recognition by Eco RI endonuclease. 
  • DNA network structures on various solid substrates investigated by atomic force microscopy. 
  • DNA prime/protein boost vaccine strategy in neonatal macaques against simian human immunodeficiency virus. 
  • DNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1. 
  • DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes. 
  • DNA vaccine molecular adjuvants SP-D-BAFF and SP-D-APRIL enhance anti-gp120 immune response and increase HIV-1 neutralizing antibody titers. 
  • De novo assembly using low-coverage short read sequence data from the rice pathogen Pseudomonas syringae pv. oryzae. 
  • Defining Transcription Regulatory Elements in the Human Frataxin Gene: Implications for Gene Therapy. 
  • Deletion analysis of bacteriophage T4 tertiary origins. A promoter sequence is required for a rifampicin-resistant replication origin. 
  • Delineating the requirements for spontaneous DNA damage resistance pathways in genome maintenance and viability in Saccharomyces cerevisiae. 
  • Derivation and characterization of a simian immunodeficiency virus SIVmac239 variant with tropism for CXCR4. 
  • Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs 
  • Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair. 
  • Detecting ultraviolet damage in single DNA molecules by atomic force microscopy. 
  • Detection of DNA looping due to simultaneous interaction of a DNA-binding protein with two spatially separated binding sites on DNA. 
  • Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus. 
  • Development of a high-efficiency method for gene marking of Dunning prostate cancer cell lines with the enzyme beta-galactosidase. 
  • Development of a novel screen for protease inhibitors. 
  • Development of resistance in Pseudomonas aeruginosa to imipenem, norfloxacin, and ciprofloxacin during therapy: proof provided by typing with a DNA probe. 
  • Differences in Methadone Metabolism by CYP2B6 Variants. 
  • Different secretory pathways of renin from mouse cells transfected with the human renin gene. 
  • Differential expression of intracellular and secreted osteopontin isoforms by murine macrophages in response to toll-like receptor agonists. 
  • Differential requirement for STAT by gain-of-function and wild-type receptor tyrosine kinase Torso in Drosophila. 
  • Differential sensitivity of chromium-mediated DNA interstrand crosslinks and DNA-protein crosslinks to disruption by alkali and EDTA. 
  • Differential transcriptional activation of peroxisome proliferator-activated receptor gamma by omega-3 and omega-6 fatty acids in MCF-7 cells. 
  • Dioxin induces transcription of fos and jun genes by Ah receptor-dependent and -independent pathways. 
  • Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules. 
  • Disruption of a topoisomerase-DNA cleavage complex by a DNA helicase. 
  • Dissemination of an Enterococcus Inc18-Like vanA plasmid associated with vancomycin-resistant Staphylococcus aureus. 
  • Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis. 
  • Domain organization of chicken gizzard myosin light chain kinase deduced from a cloned cDNA. 
  • Double-strand break repair in tandem repeats during bacteriophage T4 infection. 
  • Down-regulation of neu/HER-2 by interferon-gamma in prostate cancer cells. 
  • Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation. 
  • Dynamic shuttling and intranuclear mobility of nuclear hormone receptors. 
  • Effect of carbon source addition on toluene biodegradation by an Escherichia coli DH5alpha transconjugant harboring the TOL plasmid. 
  • Effect of mutations in genes affecting homologous recombination on restriction enzyme-mediated and illegitimate recombination in Saccharomyces cerevisiae. 
  • Effect of plasmid DNA vaccine design and in vivo electroporation on the resulting vaccine-specific immune responses in rhesus macaques. 
  • Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity? 
  • Effective tumor therapy with plasmid-encoded cytokines combined with in vivo electroporation. 
  • Effects of glutathione on chromium-induced DNA crosslinking and DNA polymerase arrest. 
  • Effects of pulse strength and pulse duration on in vitro DNA electromobility. 
  • Efficient gene transfer with adeno-associated virus-based plasmids complexed to cationic liposomes for gene therapy of human prostate cancer. 
  • Efficient in vivo gene transfer into the heart in the rat myocardial infarction model using the HVJ (Hemagglutinating Virus of Japan)--liposome method. 
  • Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo. 
  • Electromobility of plasmid DNA in tumor tissues during electric field-mediated gene delivery. 
  • Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells. 
  • Engineered leucine zippers show that hemiphosphorylated CREB complexes are transcriptionally active. 
  • Engineering biosynthetic excitable tissues from unexcitable cells for electrophysiological and cell therapy studies. 
  • Enhanced avidity maturation of antibody to human immunodeficiency virus envelope: DNA vaccination with gp120-C3d fusion proteins. 
  • Enhanced gene expression of systemically administered plasmid DNA in the liver with therapeutic ultrasound and microbubbles. 
  • Enhanced potency of plasmid DNA microparticle human immunodeficiency virus vaccines in rhesus macaques by using a priming-boosting regimen with recombinant proteins. 
  • Enhancement of antibodies to the human immunodeficiency virus type 1 envelope by using the molecular adjuvant C3d. 
  • Enhancer-blocking activity within the DNase I hypersensitive site 2 to 6 region between the TCR alpha and Dad1 genes. 
  • Environmental and genetic determinants of plasmid mobility in pathogenic Escherichia coli. 
  • Epigenetic activation of unintegrated HIV-1 genomes by gut-associated short chain fatty acids and its implications for HIV infection. 
  • Evaluating and enhancing ribozyme reaction efficiency in mammalian cells. 
  • Evaluation of a recombinant yeast cell estrogen screening assay. 
  • Evasion of immune responses to introduced human acid alpha-glucosidase by liver-restricted expression in glycogen storage disease type II. 
  • Evidence for a common mechanism of action for antitumor and antibacterial agents that inhibit type II DNA topoisomerases. 
  • Evidence for positive and negative regulatory elements in the 5'-flanking sequence of the mouse sparc (osteonectin) gene. 
  • Excellent safety and tolerability of the human immunodeficiency virus type 1 pGA2/JS2 plasmid DNA priming vector vaccine in HIV type 1 uninfected adults. 
  • Expansions and contractions of the genetic map relative to the physical map of yeast chromosome III. 
  • Expression and sequence analysis of a cDNA encoding the orotidine-5'-monophosphate decarboxylase domain from Ehrlich ascites uridylate synthase. 
  • Expression and site-directed mutagenesis of the phosphatidylcholine-preferring phospholipase C of Bacillus cereus: probing the role of the active site Glu146. 
  • Expression cloning of three Rhizobium leguminosarum lipopolysaccharide core galacturonosyltransferases. 
  • Expression of SV40 and polyoma T antigens in E. coli. 
  • Expression of a constitutive form of calcium/calmodulin dependent protein kinase II leads to arrest of the cell cycle in G2. 
  • Expression of a peptide inhibitor of protein phosphatase 1 increases phosphorylation and activity of CREB in NIH 3T3 fibroblasts. 
  • Expression of cDNAs for G proteins in Escherichia coli. Two forms of Gs alpha stimulate adenylate cyclase. 
  • Expression of functional chicken oviduct progesterone receptors in yeast (Saccharomyces cerevisiae). 
  • Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functions. 
  • Expression of microtubule motor proteins in bacteria for characterization in in vitro motility assays. 
  • Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons. 
  • Extent of equilibrium perturbation of the DNA helix upon enzymatic methylation of adenine residues. 
  • Extracellular secretion of pectate lyase by the Erwinia chrysanthemi out pathway is dependent upon Sec-mediated export across the inner membrane. 
  • Facilitated diffusion during catalysis by EcoRI endonuclease. Nonspecific interactions in EcoRI catalysis. 
  • FimC is a periplasmic PapD-like chaperone that directs assembly of type 1 pili in bacteria. 
  • Finishing the euchromatic sequence of the human genome. 
  • First somatic mutation of E2F1 in a critical DNA binding residue discovered in well-differentiated papillary mesothelioma of the peritoneum. 
  • Flanking nuclear matrix attachment regions synergize with the T cell receptor delta enhancer to promote V(D)J recombination. 
  • Formation of a rate-limiting intermediate in 5S RNA gene transcription. 
  • Formation of supercoiling domains in plasmid pBR322. 
  • Formulations which increase the size of lipoplexes prevent serum-associated inhibition of transfection. 
  • Frequent engagement of the classical and alternative NF-kappaB pathways by diverse genetic abnormalities in multiple myeloma. 
  • Function of the hydrophilic carboxyl terminus of type II DNA topoisomerase from Drosophila melanogaster. II. In vivo studies. 
  • Functional domains of the human vitamin D3 receptor regulate osteocalcin gene expression. 
  • Functional expression of the multidrug resistance-associated protein in the yeast Saccharomyces cerevisiae. 
  • Functional significance of the central helix in calmodulin. 
  • Functionality of the TOL plasmid under varying environmental conditions following conjugal transfer. 
  • Functions of fission yeast orp2 in DNA replication and checkpoint control. 
  • Fusigenic liposome-mediated DNA transfer into cardiac myocytes. 
  • Fusions of elastin-like polypeptides to pharmaceutical proteins. 
  • G protein-coupled receptor kinase and beta-arrestin-mediated desensitization of the angiotensin II type 1A receptor elucidated by diacylglycerol dynamics. 
  • G protein-coupled receptor kinase regulates dopamine D3 receptor signaling by modulating the stability of a receptor-filamin-beta-arrestin complex. A case of autoreceptor regulation. 
  • G-protein-coupled receptor genes as protooncogenes: constitutively activating mutation of the alpha 1B-adrenergic receptor enhances mitogenesis and tumorigenicity. 
  • GIPC interacts with the beta1-adrenergic receptor and regulates beta1-adrenergic receptor-mediated ERK activation. 
  • Gene delivery approaches to heart failure treatment. 
  • Gene delivery systems in surgery. 
  • Gene delivery through cell culture substrate adsorbed DNA complexes 
  • Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans. 
  • Gene modification of primary tumor cells for active immunotherapy of human breast and ovarian cancer. 
  • Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division. 
  • Gene silencing using RNA interference in embryonic stem cells. 
  • Gene therapy and genomic strategies for cardiovascular surgery: The emerging field of surgiomics. 
  • Gene transfer and expression in rat cardiac transplants. 
  • Gene transfer to the thymus. A means of abrogating the immune response to recombinant adenovirus. 
  • Generating mammalian sirtuin tools for protein-interaction analysis. 
  • Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach. 
  • Generation of recombinant vaccinia virus and analysis of virus-induced cell death. 
  • Genetic bioaugmentation as an effective method for in situ bioremediation: functionality of catabolic plasmids following conjugal transfers. 
  • Genetic manipulation of centromere function. 
  • Genetic requirements for spontaneous and transcription-stimulated mitotic recombination in Saccharomyces cerevisiae. 
  • Genome nucleotide lengths that are divisible by six are not essential but enhance replication of defective interfering RNAs of the paramyxovirus simian virus 5. 
  • Genomic Analysis of Multidrug-Resistant Escherichia coli from North Carolina Community Hospitals: Ongoing Circulation of CTX-M-Producing ST131-H30Rx and ST131-H30R1 Strains. 
  • Genomic organization of rat rDNA. 
  • Glu-111 is required for activation of the DNA cleavage center of EcoRI endonuclease. 
  • Gonadotropin regulation of the rat proopiomelanocortin promoter: characterization by transfection of primary ovarian granulosa cells. 
  • Group I permuted intron-exon (PIE) sequences self-splice to produce circular exons. 
  • Growth hormone regulates phosphorylation and function of CCAAT/enhancer-binding protein beta by modulating Akt and glycogen synthase kinase-3. 
  • Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. 
  • Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae. 
  • High level expression of a truncated chicken progesterone receptor in Escherichia coli. 
  • High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae. 
  • High level expression of mammalian protein farnesyltransferase in a baculovirus system. The purified protein contains zinc. 
  • High-Efficiency Cellular Reprogramming by Nanoscale Puncturing. 
  • High-level expression and single-step purification of leucyl-tRNA synthetase from Escherichia coli. 
  • High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor. 
  • Horizontal gene transfer enables programmable gene stability in synthetic microbiota. 
  • Human sulfite oxidase R160Q: identification of the mutation in a sulfite oxidase-deficient patient and expression and characterization of the mutant enzyme. 
  • Hypermethylation of growth arrest DNA damage-inducible gene 45 beta promoter in human hepatocellular carcinoma. 
  • Hyperosmotic stress stimulates promoter activity and regulates cellular utilization of the serum- and glucocorticoid-inducible protein kinase (Sgk) by a p38 MAPK-dependent pathway. 
  • Hypoxia impedes the formation of chromium DNA-adducts in a cell-free system. 
  • Identification and cell type specificity of the tyrosine hydroxylase gene promoter. 
  • Identification and sequence of the initiation site for rat 45S ribosomal RNA synthesis. 
  • Identification of a motif in the carboxyl terminus of beta -arrestin2 responsible for activation of JNK3. 
  • Identification of a muscle-specific enhancer within the 5'-flanking region of the human myoglobin gene. 
  • Identification of a negative regulatory function for steroid receptors. 
  • Identification of an estrogen response element in the 3'-flanking region of the murine c-fos protooncogene. 
  • Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of angiotensin II-mediated nuclear factor of activated T cells (NFAT) activation. 
  • Identification of potassium-dependent and -independent components of the apoptotic machinery in mouse ovarian germ cells and granulosa cells. 
  • Identification of the TBX5 transactivating domain and the nuclear localization signal. 
  • Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis. 
  • Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis. 
  • Identifying sequence similarities between DNA molecules. 
  • Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells. 
  • Impacts of organic carbon availability and recipient bacteria characteristics on the potential for TOL plasmid genetic bioaugmentation in soil slurries. 
  • In vitro assay of immunostimulatory activities of plasmid vectors. 
  • In vitro assembly studies of FtsZ/tubulin-like proteins (TubZ) from Bacillus plasmids: evidence for a capping mechanism. 
  • In vitro cell cycle arrest induced by using artificial DNA templates. 
  • In vivo mutagenesis of the reporter plasmid pSP189 induced by exposure of host Ad293 cells to activated polymorphonuclear leukocytes. 
  • In-vivo studies on the cis-acting replication initiator protein of IncFII plasmid NR1. 
  • Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease. 
  • Incompatibility and IncFII plasmid replication control. 
  • Increased immunogenicity of a DNA-launched Venezuelan equine encephalitis virus-based replicon DNA vaccine. 
  • Increased resistance of tumor cells to hyperthermia mediated by integrin-linked kinase. 
  • Induction of CD4(+) and CD8(+) T-cell responses to the human stromal antigen, fibroblast activation protein: implication for cancer immunotherapy. 
  • Inhibition of G protein-coupled receptor signaling by expression of cytoplasmic domains of the receptor. 
  • Inositol diphosphate signaling regulates telomere length. 
  • Instability of rRNA operons in Bacillus subtilis. 
  • Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. 
  • Integration of plasmids into the bacteriophage T4 genome. 
  • Interaction between transcriptional activator protein LAC9 and negative regulatory protein GAL80. 
  • Interleukin 1 regulates synthesis of amyloid beta-protein precursor mRNA in human endothelial cells. 
  • Interrupter sequences that are widely distributed in the Drosophila genome. 
  • Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection. 
  • Intracellular mechanisms of gonadotropin-stimulated gene expression in granulosa cells. 
  • Intrachromosomal gene conversion in yeast. 
  • Intraspecific phylogenetic congruence among multiple symbiont genomes. 
  • Intratumoral Plasmid IL12 Expands CD8+ T Cells and Induces a CXCR3 Gene Signature in Triple-negative Breast Tumors that Sensitizes Patients to Anti-PD-1 Therapy. 
  • Intrinsic and cyclin-dependent kinase-dependent control of spindle pole body duplication in budding yeast. 
  • Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. 
  • Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection. 
  • Involvement of an ATP-dependent peptide chaperone in cross-presentation after DNA immunization. 
  • Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence. 
  • Isolation and analysis of recombinant DNA molecules containing yeast DNA. 
  • Isolation and characterization of effector-loop mutants of CDC42 in yeast. 
  • Isolation and characterization of the replicon of a Thiobacillus intermedius plasmid. 
  • Isolation and functional analysis of the mouse RXRgamma1 gene promoter in anterior pituitary cells. 
  • Isolation of gram quantities of EcoRI restriction and modification enzymes from an overproducing strain. 
  • Isolation of human sequences that replicate autonomously in human cells. 
  • Isolation of retinal proteins that interact with retinitis pigmentosa GTPase regulator by interaction trap screen in yeast. 
  • Isolation of song-regulated genes in the brain of songbirds. 
  • Isolation of the structural gene for calmodulin. 
  • Juglone, an inhibitor of the peptidyl-prolyl isomerase Pin1, also directly blocks transcription. 
  • Left-handed DNA helices, supercoiling, and the B-Z junction. 
  • Ligand-specific dynamics of the androgen receptor at its response element in living cells. 
  • Long-term correction of glycogen storage disease type II with a hybrid Ad-AAV vector. 
  • Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney. 
  • Low-frequency infection of F- bacteria by transducing particles of filamentous bacteriophages. 
  • Macrophage colony-stimulating factor can modulate immune responses and attract dendritic cells in vivo. 
  • Mapping of rRNA genes with integrable plasmids in Bacillus subtilis. 
  • Mating pair formation homologue TraG is a variable membrane protein essential for contact-independent type IV secretion of chromosomal DNA by Neisseria gonorrhoeae. 
  • Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain. 
  • Mechanism of specific site location and DNA cleavage by EcoR I endonuclease. 
  • Mechanism of staphylococcal multiresistance plasmid replication origin assembly by the RepA protein. 
  • Mechanisms of chromium-induced suppression of RNA synthesis in cellular and cell-free systems: relationship to RNA polymerase arrest. 
  • MegaGate: A toxin-less gateway molecular cloning tool. 
  • Meiosis-specific double-strand DNA breaks at the HIS4 recombination hot spot in the yeast Saccharomyces cerevisiae: control in cis and trans. 
  • Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cells. 
  • Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycerol-3-phosphate acyl/transferase. 
  • Membrane phospholipid synthesis in Escherichia coli. Identification of the sn-glycerol-3-phosphate acyltransferase polypeptide as the plsB gene product. 
  • Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli. 
  • Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II. 
  • Modification of lupus-associated 60-kDa Ro protein with the lipid oxidation product 4-hydroxy-2-nonenal increases antigenicity and facilitates epitope spreading. 
  • Modulation of phagosome biogenesis by Legionella pneumophila creates an organelle permissive for intracellular growth. 
  • Molecular Analysis of pSK1 par: A Novel Plasmid Partitioning System Encoded by Staphylococcal Multiresistance Plasmids. 
  • Molecular analysis of the Cryptococcus neoformans ADE2 gene, a selectable marker for transformation and gene disruption. 
  • Molecular cloning and expression of the cDNA for the alpha 1A-adrenergic receptor. The gene for which is located on human chromosome 5. 
  • Molecular cloning of ILP-2, a novel member of the inhibitor of apoptosis protein family. 
  • Molecular cloning of the gene sequences of a major apoprotein in avian very low density lipoproteins. 
  • Molecular definition of a novel inositol polyphosphate metabolic pathway initiated by inositol 1,4,5-trisphosphate 3-kinase activity in Saccharomyces cerevisiae. 
  • Molecular determinants in the second intracellular loop of the 5-hydroxytryptamine-1A receptor for G-protein coupling. 
  • Molecular determinants underlying the formation of stable intracellular G protein-coupled receptor-beta-arrestin complexes after receptor endocytosis*. 
  • Molecular genetics of yeast. 
  • Molecular structure and flanking nucleotide sequences of the natural chicken ovomucoid gene. 
  • Multiple gene segments control the temperature sensitivity and attenuation phenotypes of ca B/Ann Arbor/1/66. 
  • Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy. 
  • Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I. 
  • Mutagenesis by exocyclic alkylamino purine adducts in Escherichia coli. 
  • Mutagenicity of ochratoxin A and its hydroquinone metabolite in the SupF gene of the mutation reporter plasmid Ps189. 
  • Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition. 
  • Mutation detection with MutH, MutL, and MutS mismatch repair proteins. 
  • Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells. 
  • Mutations that affect Tn5 insertion into pBR322: importance of local DNA supercoiling. 
  • Myosin light chain kinase structure function analysis using bacterial expression. 
  • N-(6-chloro-pyridin-3-yl)-3,4-difluoro-benzamide (ICA-27243): a novel, selective KCNQ2/Q3 potassium channel activator. 
  • Nanoscale detection of ionizing radiation damage to DNA by atomic force microscopy. 
  • Nerve activity-dependent modulation of calcineurin signaling in adult fast and slow skeletal muscle fibers. 
  • Non-function of a Moloney murine leukaemia virus regulatory sequence in F9 embryonal carcinoma cells. 
  • Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice 
  • Norfloxacin-induced DNA gyrase cleavage complexes block Escherichia coli replication forks, causing double-stranded breaks in vivo. 
  • Novel insulinoma cell lines produced by iterative engineering of GLUT2, glucokinase, and human insulin expression. 
  • Nuclear beta-catenin-dependent Wnt8 signaling in vegetal cells of the early sea urchin embryo regulates gastrulation and differentiation of endoderm and mesodermal cell lineages. 
  • Nucleotide excision repair functions in the removal of chromium-induced DNA damage in mammalian cells. 
  • O-glycosylation as a sorting determinant for cell surface delivery in yeast. 
  • Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells. 
  • Optimization of expression of human sulfite oxidase and its molybdenum domain. 
  • Optimizing delivery for efficient cardiac reprogramming. 
  • Overexpressed heat shock protein 70 protects cells against DNA damage caused by ultraviolet C in a dose-dependent manner. 
  • P pili in uropathogenic E. coli are composite fibres with distinct fibrillar adhesive tips. 
  • P1 partition complex assembly involves several modes of protein-DNA recognition. 
  • PKA-mediated phosphorylation of the beta1-adrenergic receptor promotes Gs/Gi switching. 
  • Palmitoylation of G protein-coupled receptor kinase, GRK6. Lipid modification diversity in the GRK family. 
  • Pathways Governing Polyethylenimine Polyplex Transfection in Microporous Annealed Particle Scaffolds. 
  • Persistence and reversal of plasmid-mediated antibiotic resistance. 
  • Plasmid DNA network on a mica substrate investigated by atomic force microscopy. 
  • Plasmid models for bacteriophage T4 DNA replication: requirements for fork proteins. 
  • Plasmid protein TubR uses a distinct mode of HTH-DNA binding and recruits the prokaryotic tubulin homolog TubZ to effect DNA partition. 
  • Plasmids for recombination-based screening. 
  • Plasticity of lipid-protein interactions in the function and topogenesis of the membrane protein lactose permease from Escherichia coli. 
  • Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model 
  • Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae. 
  • Positive regulation of the vitamin D receptor by its cognate ligand in heterologous expression systems. 
  • Positive-selection cloning vehicle useful for overproduction of hybrid proteins. 
  • Precise arrangement of factor-binding sites is required for murine CD4 promoter function. 
  • Predicting plasmid persistence in microbial communities by coarse-grained modeling. 
  • Preserved CD4+ central memory T cells and survival in vaccinated SIV-challenged monkeys. 
  • Primary sequence of ovomucoid messenger RNA as determined from cloned complementary DNA. 
  • Priming with plasmid DNAs expressing interleukin-12 and simian immunodeficiency virus gag enhances the immunogenicity and efficacy of an experimental AIDS vaccine based on recombinant vesicular stomatitis virus. 
  • Production of highly potent recombinant siRNAs in Escherichia coli. 
  • Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. 
  • Programmed population control by cell-cell communication and regulated killing. 
  • Properties affecting transfer and expression of degradative plasmids for the purpose of bioremediation. 
  • Protection against Mycobacterium avium by DNA vaccines expressing mycobacterial antigens as fusion proteins with green fluorescent protein. 
  • Protective immunity to SIV challenge elicited by vaccination of macaques with multigenic DNA vaccines producing virus-like particles. 
  • Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking. 
  • Protein phosphatase 5 is required for ATR-mediated checkpoint activation. 
  • Purification of a 12,020-dalton protein that enhances the activation of mitogen-activated protein (MAP) kinase by MAP kinase kinase. 
  • Purification of the HhaII restriction endonuclease from an overproducer Escherichia coli clone. 
  • Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins. 
  • Pyruvate aldolases in chiral carbon-carbon bond formation. 
  • Quadruplex DNA formation in a region of the tRNA gene supF associated with hydrogen peroxide mediated mutations. 
  • RCC1, a regulator of mitosis, is essential for DNA replication. 
  • RU486 exerts antiestrogenic activities through a novel progesterone receptor A form-mediated mechanism. 
  • Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing. 
  • Rapid memory CD8+ T-lymphocyte induction through priming with recombinant Mycobacterium smegmatis. 
  • Receptor-transporting protein 1 short (RTP1S) mediates translocation and activation of odorant receptors by acting through multiple steps. 
  • Recombinant immunotoxin containing a disulfide-stabilized Fv directed at erbB2 that does not require proteolytic activation. 
  • Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity. 
  • Recombination-dependent DNA replication stimulated by double-strand breaks in bacteriophage T4. 
  • Recombination-dependent replication of plasmids during bacteriophage T4 infection. 
  • Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes. 
  • Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. 
  • Regions of the alpha 1-adrenergic receptor involved in coupling to phosphatidylinositol hydrolysis and enhanced sensitivity of biological function. 
  • Regulation of MEF2 by histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications. 
  • Regulation of arrestin-3 phosphorylation by casein kinase II. 
  • Regulation of both PDK1 and the phosphorylation of PKC-zeta and -delta by a C-terminal PRK2 fragment. 
  • Regulation of casein kinase I epsilon activity by Wnt signaling. 
  • Regulation of transcription of the repA1 gene in the replication control region of IncFII plasmid NR1 by gene dosage of the repA2 transcription repressor protein. 
  • Relationships among DNA sequences of the 1.3 kb EcoRI family of mouse DNA. 
  • Release of amino-terminal fragments from amyloid precursor protein reporter and mutated derivatives in cultured cells. 
  • Remodeling Yersinia pseudotuberculosis to generate a highly immunogenic outer membrane vesicle vaccine against pneumonic plague. 
  • Repair of double-strand breaks in bacteriophage T4 by a mechanism that involves extensive DNA replication. 
  • Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease. 
  • Repair of tobacco carcinogen-induced DNA adducts and lung cancer risk: a molecular epidemiologic study. 
  • Repair of topoisomerase-mediated DNA damage in bacteriophage T4. 
  • Replication control of autonomously replicating human sequences. 
  • Replication of N2-ethyldeoxyguanosine DNA adducts in the human embryonic kidney cell line 293. 
  • Replication-attenuated Human Adenoviral Type 4 vectors elicit capsid dependent enhanced innate immune responses that are partially dependent upon interactions with the complement system. 
  • Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins. 
  • Requirement for Abl kinases in T cell receptor signaling. 
  • Rhizobium gone native: unexpected plasmid stability of indigenous Rhizobium leguminosarum. 
  • Role of arginine 180 and glutamic acid 177 of ricin toxin A chain in enzymatic inactivation of ribosomes. 
  • Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in Schizosaccharomyces pombe. 
  • Role of the aryl hydrocarbon receptor in cell cycle regulation. 
  • Rvs161p and sphingolipids are required for actin repolarization following salt stress. 
  • Safety and immunogenicity of an HIV adenoviral vector boost after DNA plasmid vaccine prime by route of administration: a randomized clinical trial. 
  • Safety and immunogenicity of cytotoxic T-lymphocyte poly-epitope, DNA plasmid (EP HIV-1090) vaccine in healthy, human immunodeficiency virus type 1 (HIV-1)-uninfected adults. 
  • Screening of HIV-1 Env glycoproteins for the ability to raise neutralizing antibody using DNA immunization and recombinant vaccinia virus boosting. 
  • Secondary structure of the self-cleaving RNA of hepatitis delta virus: applications to catalytic RNA design. 
  • Segrosome structure revealed by a complex of ParR with centromere DNA. 
  • Selection of an RNA molecule that mimics a major autoantigenic epitope of human insulin receptor. 
  • Selective LXXLL peptides antagonize transcriptional activation by the retinoid-related orphan receptor RORgamma. 
  • Selective suppression of matrix metalloproteinase-9 in human glioblastoma cells by antisense gene transfer impairs glioblastoma cell invasion. 
  • Separating DNA with different topologies by atomic force microscopy in comparison with gel electrophoresis. 
  • Sequence length required for homologous recombination in Cryptococcus neoformans. 
  • Sequences within the spacer region of yeast rRNA cistrons that stimulate 35S rRNA synthesis in vivo mediate RNA polymerase I-dependent promoter and terminator activities. 
  • Seven-base-pair inverted repeats in DNA form stable hairpins in vivo in Saccharomyces cerevisiae. 
  • Site-specific casein kinase 1epsilon-dependent phosphorylation of Dishevelled modulates beta-catenin signaling. 
  • Site-specific integration of the Haemophilus influenzae bacteriophage HP1. Identification of the points of recombinational strand exchange and the limits of the host attachment site. 
  • Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site. 
  • Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. 
  • Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo. 
  • Soluble Fas ligand blocks destructive corneal inflammation in mouse models of corneal epithelial debridement and LPS induced keratitis. 
  • Spatiotemporal modulation of biodiversity in a synthetic chemical-mediated ecosystem. 
  • Specific 33-residue repeat(s) of erythrocyte ankyrin associate with the anion exchanger. 
  • Specific transforming potential of oncogenes encoding protein-tyrosine kinases. 
  • Specificity of human bactericidal antibodies against PorA P1.7,16 induced with a hexavalent meningococcal outer membrane vesicle vaccine. 
  • Stabilization of microsatellite sequences by variant repeats in the yeast Saccharomyces cerevisiae. 
  • Steroid hormone receptors selectively affect transcriptional activation but not basal repression by thyroid hormone receptors. 
  • Stimulation and inhibition of 1,3-bis(2-chloroethyl)-1-nitrosourea-induced strand breaks and interstrand cross-linking in Col E1 plasmid deoxyribonucleic acid by polyamines and inorganic cations. 
  • Stimulation of GCMa transcriptional activity by cyclic AMP/protein kinase A signaling is attributed to CBP-mediated acetylation of GCMa. 
  • Stoichiometric preference in copper-promoted oxidative DNA damage by ochratoxin A. 
  • Structural and Functional Analyses Reveal Insights into the Molecular Properties of the Escherichia coli Z Ring Stabilizing Protein, ZapC. 
  • Structural and functional studies of cyclic GMP phosphodiesterase. 
  • Structural basis for ADP-mediated transcriptional regulation by P1 and P7 ParA. 
  • Structural biology of plasmid partition: uncovering the molecular mechanisms of DNA segregation. 
  • Structural biology of plasmid segregation proteins. 
  • Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation. 
  • Structure and filament dynamics of the pSK41 actin-like ParM protein: implications for plasmid DNA segregation. 
  • Structure of the Z Ring-associated Protein, ZapD, Bound to the C-terminal Domain of the Tubulin-like Protein, FtsZ, Suggests Mechanism of Z Ring Stabilization through FtsZ Cross-linking. 
  • Studies of the neutralizing activity and avidity of anti-human immunodeficiency virus type 1 Env antibody elicited by DNA priming and protein boosting. 
  • Studies of the regulatory mechanism of Ca2+/calmodulin-dependent protein kinase II. Mutation of threonine 286 to alanine and aspartate. 
  • Study of the kinetic and physical properties of the orotidine-5'-monophosphate decarboxylase domain from mouse UMP synthase produced in Saccharomyces cerevisiae. 
  • Superimmunity: characterization of a new gene in the immunity region of P1. 
  • Surface modified poly(β amino ester)-containing nanoparticles for plasmid DNA delivery. 
  • Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles. 
  • Surfactant protein A is defective in abrogating inflammation in asthma. 
  • Sustained in vivo gene delivery from agarose hydrogel prolongs nonviral gene expression in skin. 
  • Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel. 
  • Synergistic and tunable human gene activation by combinations of synthetic transcription factors. 
  • Synthesis of DNA coding for human proinsulin. 
  • Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells. 
  • Tandemly arranged variant 5S ribosomal RNA genes in the yeast Saccharomyces cerevisiae. 
  • Targeted DNA recombination in vivo using an adenovirus carrying the cre recombinase gene. 
  • Targeted plasmid integration into the human genome by an engineered zinc-finger recombinase. 
  • Temporal control of self-organized pattern formation without morphogen gradients in bacteria. 
  • The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae. 
  • The Caenorhabditis elegans ABL-1 tyrosine kinase is required for Shigella flexneri pathogenesis. 
  • The Haemophilus influenzae Hia autotransporter contains an unusually short trimeric translocator domain. 
  • The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins. 
  • The SV40 core sequence functions as a repressor element in yeast. 
  • The Saccharomyces cerevisiae suppressor of choline sensitivity (SCS2) gene is a multicopy Suppressor of mec1 telomeric silencing defects. 
  • The Staphylococcus aureus pSK41 plasmid-encoded ArtA protein is a master regulator of plasmid transmission genes and contains a RHH motif used in alternate DNA-binding modes. 
  • The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation. 
  • The Tn3 beta-lactamase gene acts as a hotspot for meiotic recombination in yeast. 
  • The anaphase-promoting complex/cyclosome is required for anaphase progression in multinucleated Ashbya gossypii cells. 
  • The ankyrin-B C-terminal domain determines activity of ankyrin-B/G chimeras in rescue of abnormal inositol 1,4,5-trisphosphate and ryanodine receptor distribution in ankyrin-B (-/-) neonatal cardiomyocytes. 
  • The bacteriophage T4 insertion/substitution vector system. A method for introducing site-specific mutations into the virus chromosome. 
  • The chick ovomucoid gene contains at least six intervening sequences. 
  • The genetic determinant of adhesive function in type 1 fimbriae of Escherichia coli is distinct from the gene encoding the fimbrial subunit. 
  • The homophilic adhesion molecule sidekick-1 contributes to augmented podocyte aggregation in HIV-associated nephropathy. 
  • The inhibitor-1 C terminus facilitates hormonal regulation of cellular protein phosphatase-1: functional implications for inhibitor-1 isoforms. 
  • The integrity of a cholesterol-binding pocket in Niemann-Pick C2 protein is necessary to control lysosome cholesterol levels. 
  • The kinetic mechanism of EcoRI endonuclease. 
  • The need for enhancers in gene expression first appears during mouse development with formation of the zygotic nucleus. 
  • The neuronal actin-binding proteins, neurabin I and neurabin II, recruit specific isoforms of protein phosphatase-1 catalytic subunits. 
  • The opposing transcriptional activities of the two isoforms of the human progesterone receptor are due to differential cofactor binding. 
  • The persistence potential of transferable plasmids. 
  • The retinitis pigmentosa GTPase regulator (RPGR) interacts with novel transport-like proteins in the outer segments of rod photoreceptors. 
  • The strength and periodicity of D. melanogaster circadian rhythms are differentially affected by alterations in period gene expression. 
  • The structure of the trpE, trpD and 5' trpC genes of Bacillus pumilus. 
  • The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth. 
  • The tight linkage between DNA replication and double-strand break repair in bacteriophage T4. 
  • The utrophin A 5'-untranslated region confers internal ribosome entry site-mediated translational control during regeneration of skeletal muscle fibers. 
  • Thermodynamic parameters governing interaction of EcoRI endonuclease with specific and nonspecific DNA sequences. 
  • Three amino acid substitutions in domain I of calmodulin prevent the activation of chicken smooth muscle myosin light chain kinase. 
  • Threonine biosynthetic genes are essential in Cryptococcus neoformans. 
  • Thymic function after hematopoietic stem-cell transplantation for the treatment of severe combined immunodeficiency. 
  • Timing of plasmid cytokine (IL-2/Ig) administration affects HIV-1 vaccine immunogenicity in HIV-seronegative subjects. 
  • Tn5 insertion specificity is not influenced by IS50 end sequences in target DNA. 
  • Topoisomerase I is essential in Cryptococcus neoformans: role In pathobiology and as an antifungal target. 
  • Trafficking of yellow-fluorescent-protein-tagged mu1 subunit of clathrin adaptor AP-1 complex in living cells. 
  • Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination. 
  • Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA. 
  • Transactivation of BKV and SV40 early promoters by BKV and SV40 T-antigens. 
  • Transcription inhibits the replication of autonomously replicating plasmids in human cells. 
  • Transcription of orthopoxvirus telomeres at late times during infection. 
  • Transcription-associated mutation of lasR in Pseudomonas aeruginosa. 
  • Transcriptional network of multiple capsule and melanin genes governed by the Cryptococcus neoformans cyclic AMP cascade. 
  • Transcriptional pausing in a region important for plasmid NR1 replication control. 
  • Transfection of a human cytochrome P-450 gene into the human lymphoblastoid cell line, AHH-1, and use of the recombinant cell line in gene mutation assays. 
  • Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences. 
  • Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector. 
  • Transgenic indicator mice for studying activated retinoic acid receptors during development. 
  • Transgenic manipulation of the metabolism of polyamines in poplar cells. 
  • Translation by the adenovirus tripartite leader: elements which determine independence from cap-binding protein complex. 
  • Translational inhibition of messenger RNA of the human pi class glutathione S-transferase by antisense oligodeoxyribonucleotides. 
  • Transposon Tn5 target specificity: preference for insertion at G/C pairs. 
  • Triplet repeats form secondary structures that escape DNA repair in yeast. 
  • Tumor necrosis factor-alpha is a potent endogenous mutagen that promotes cellular transformation. 
  • Two mouse early embryonic beta-globin gene sequences. Evolution of the nonadult beta-globins. 
  • Two types of recombination hotspots in bacteriophage T4: one requires DNA damage and a replication origin and the other does not. 
  • Two-dimensional gel analysis of rolling circle replication in the presence and absence of bacteriophage T4 primase. 
  • UVA generates pyrimidine dimers in DNA directly. 
  • Ultrastructural Analysis of Vesicular Transport in Electrotransfection. 
  • Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. 
  • Utilizing cell–matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels 
  • VIAF, a conserved inhibitor of apoptosis (IAP)-interacting factor that modulates caspase activation. 
  • Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein. 
  • Versatile biomanufacturing through stimulus-responsive cell-material feedback. 
  • Vertical and horizontal gene transfer tradeoffs direct plasmid fitness. 
  • Vertical transmission of biosynthetic plasmids in aphid endosymbionts (Buchnera). 
  • Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. 
  • Vitamin D receptor interaction with specific DNA requires a nuclear protein and 1,25-dihydroxyvitamin D3. 
  • WT1 and DAX-1 regulate SF-1-mediated human P450arom gene expression in gonadal cells. 
  • Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ. 
  • [Expression of recombinant human hemangiopoietin and preparation of its polyclonal antibody]. 
  • [Synthesis of human proinsulin in Escherichia coli cells]. 
  • beta-arrestin-dependent, G protein-independent ERK1/2 activation by the beta2 adrenergic receptor. 
  • c-IAP1 is cleaved by caspases to produce a proapoptotic C-terminal fragment. 
• 

  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Ferrari, Guido, Professor in Surgery, Molecular Genetics and Microbiology
  • Ferreira, Paulo Alexandre, Associate Professor in Ophthalmology, Pathology
  • Hartemink, Alexander J., Professor in the Department of Computer Science, Biology
  • Hirschey, Matthew, Associate Professor of Medicine, Cell Biology
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology
  • Kelsoe, Garnett H., James B. Duke Distinguished Professor of Immunology, Integrative Immunobiology
  • Krangel, Michael S., George Barth Geller Distinguished Professor of Immunology, Integrative Immunobiology
  • Shinohara, Mari L., Professor of Integrative Immunobiology, Cell Biology
  • Tsalik, Ephraim, Adjunct Associate Professor in the Department of Medicine, Medicine, Infectious Diseases
  • Velkey, John Matthew, Assistant Professor of the Practice of Medical Education in the Department of Cell Biology, Cell Biology