Polysaccharide-Lyases

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  Subject Areas on Research

  • A biomechanical role for perlecan in the pericellular matrix of articular cartilage. 
  • Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu. 
  • Extracellular secretion of pectate lyase by the Erwinia chrysanthemi out pathway is dependent upon Sec-mediated export across the inner membrane. 
  • Heparin neutralization by recombinant platelet factor 4 and protamine. 
  • Heparin neutralization with methylene blue, hexadimethrine, or vancomycin after cardiopulmonary bypass. 
  • Heparinase I (neutralase) reversal of systemic anticoagulation. 
  • Molecular cloning of the structural gene for exopolygalacturonate lyase from Erwinia chrysanthemi EC16 and characterization of the enzyme product. 
  • Role of the Hrp pilus in type III protein secretion in Pseudomonas syringae. 
  • The Pseudomonas syringae pv. tomato HrpW protein has domains similar to harpins and pectate lyases and can elicit the plant hypersensitive response and bind to pectate. 
  • The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato. 
  • Visualization of secreted Hrp and Avr proteins along the Hrp pilus during type III secretion in Erwinia amylovora and Pseudomonas syringae.