Precipitin Tests

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  Subject Areas on Research

  • A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion. 
  • A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein. 
  • A human anti-HIV autoantibody enhances EBV transformation and HIV infection. 
  • A monoclonal IgM with antibodylike specificity for phospholipids in a patient with lymphoma. 
  • A new activity of doublecortin in recognition of the phospho-FIGQY tyrosine in the cytoplasmic domain of neurofascin. 
  • A novel isoform ratio switch of the polypyrimidine tract binding protein. 
  • A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles. 
  • A role for receptor kinases in the regulation of class II G protein-coupled receptors. Phosphorylation and desensitization of the secretin receptor. 
  • APC-mediated downregulation of beta-catenin activity involves nuclear sequestration and nuclear export. 
  • ARF1 regulates Nef-induced CD4 degradation. 
  • Activation of MEF2 by muscle activity is mediated through a calcineurin-dependent pathway. 
  • Activation of the Saccharomyces cerevisiae heat shock transcription factor under glucose starvation conditions by Snf1 protein kinase. 
  • Activation of the mitogen-activated protein kinase pathway through p75NTR: a common mechanism for the neurotrophin family. 
  • Altered expression of neurofilament subunits in diisopropyl phosphorofluoridate-treated hen spinal cord and their presence in axonal aggregations. 
  • Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. 
  • An analysis of type-C retrovirus polypeptides and their associations in the virion. 
  • Anti-synthetic peptide antibody reacting at the fusion junction of deletion-mutant epidermal growth factor receptors in human glioblastoma. 
  • Antibody binding to a conformation-dependent epitope induces L-selectin association with the detergent-resistant cytoskeleton. 
  • Antihuman plasma glutathione peroxidase antibodies: immunologic investigations to determine plasma glutathione peroxidase protein and selenium content in plasma. 
  • Assembly of an A kinase-anchoring protein-beta(2)-adrenergic receptor complex facilitates receptor phosphorylation and signaling. 
  • Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p. 
  • Association of protein kinase C(lambda) with adducin in 3T3-L1 adipocytes. 
  • Autoimmune epitopes in messenger RNA. 
  • BRCA1 is required for common-fragile-site stability via its G2/M checkpoint function. 
  • Bacterial surface association of heat-labile enterotoxin through lipopolysaccharide after secretion via the general secretory pathway. 
  • Bactericidal but not nonbactericidal C5b-9 is associated with distinctive outer membrane proteins in Neisseria gonorrhoeae. 
  • Beta(2)-adrenergic and several other G protein-coupled receptors in human atrial membranes activate both G(s) and G(i). 
  • Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. 
  • Binding of factor VIII inhibitors to discrete regions of the factor VIII C2 domain disrupt phospholipid binding. 
  • Binding of the beta2 adrenergic receptor to N-ethylmaleimide-sensitive factor regulates receptor recycling. 
  • Bioassay of plasma cholecystokinin in rats: effects of food, trypsin inhibitor, and alcohol. 
  • C-terminal region of human T cell lymphotropic virus type I (HTLVI) p19 core protein is immunogenic in humans and contains an HTLVI-specific epitope. 
  • CD19 amplification of B lymphocyte Ca2+ responses: a role for Lyn sequestration in extinguishing negative regulation. 
  • CD19-dependent B lymphocyte signaling thresholds influence skin fibrosis and autoimmunity in the tight-skin mouse. 
  • Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo. 
  • Cell-dependent posttranslational processing and secretion of recombinant mouse renin-2. 
  • Cell-type-specific repression of internal ribosome entry site activity by double-stranded RNA-binding protein 76. 
  • Characterization of PDK2 activity against protein kinase B gamma. 
  • Characterization of a CD6 ligand(s) expressed on human- and murine-derived cell lines and murine lymphoid tissues. 
  • Characterization of alpha 2-adrenergic receptor subtype-specific antibodies. 
  • Characterization of an Epstein-Barr virus receptor on human epithelial cells. 
  • Characterization of an acquired inhibitor to coagulation factor V. Antibody binding to the second C-type domain of factor V inhibits the binding of factor V to phosphatidylserine and neutralizes procoagulant activity. 
  • Characterization of cDNAs encoding the polypyrimidine tract-binding protein. 
  • Characterization of precipitating antibodies to ruminant serum and milk proteins in humans with selective IgA deficiency. 
  • Characterization of senescence- and apoptosis-dependent forms of terminin as derived from a precursor found in replicating and nonreplicating cells. 
  • Characterization of two populations of statin and the relationship of their syntheses to the state of cell proliferation. 
  • Comparative analysis of Rev function in human immunodeficiency virus types 1 and 2. 
  • Comparative analysis of the HTLV-I Rex and HIV-1 Rev trans-regulatory proteins and their RNA response elements. 
  • Complement control proteins, CD46, CD55, and CD59, as common surface constituents of human and simian immunodeficiency viruses and possible targets for vaccine protection. 
  • Constitutive arrestin-mediated desensitization of a human vasopressin receptor mutant associated with nephrogenic diabetes insipidus. 
  • Deletion analysis of recombinant human factor V. Evidence for a phosphatidylserine binding site in the second C-type domain. 
  • Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2. 
  • Differences in phosphorylation of formylpeptide and C5a chemoattractant receptors correlate with differences in desensitization. 
  • Distribution and physical properties of BCA200, a Mr 200,000 glycoprotein selectively associated with human breast cancer. 
  • Dopamine D(5) receptor immunolocalization in rat and monkey brain. 
  • Down-regulation of neu/HER-2 by interferon-gamma in prostate cancer cells. 
  • ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system. 
  • Effect of IGFBP-3 on IGF- and IGF-analogue-induced insulin-like growth factor-I receptor (IGFIR) signalling. 
  • Effect of Mts1 on the structure and activity of nonmuscle myosin II. 
  • Effect of interferon-gamma and human alpha 2-macroglobulin on peritoneal macrophage morphology and Ia antigen expression. 
  • Enzymatic generation of the amino terminus of the beta-amyloid peptide. 
  • Erbin is a protein concentrated at postsynaptic membranes that interacts with PSD-95. 
  • Estrogen-induced synthesis of ovalbumin messenger RNA and its translation in a cell-free system. 
  • Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophils. 
  • Expression of early activation antigen (CD69) during human thymic development. 
  • FKBP12-rapamycin target TOR2 is a vacuolar protein with an associated phosphatidylinositol-4 kinase activity. 
  • Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. 
  • Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA. 
  • Functional association of CD7 with phosphatidylinositol 3-kinase: interaction via a YEDM motif. 
  • Functional dissection of the HIV-1 Rev trans-activator--derivation of a trans-dominant repressor of Rev function. 
  • G-protein-coupled receptor (GPCR) kinase phosphorylation and beta-arrestin recruitment regulate the constitutive signaling activity of the human cytomegalovirus US28 GPCR. 
  • GIPC interacts with the beta1-adrenergic receptor and regulates beta1-adrenergic receptor-mediated ERK activation. 
  • Gene expression analysis of messenger RNP complexes. 
  • Genetic cause of a juvenile form of Tay-Sachs disease in a Lebanese child. 
  • Genome-wide regulatory analysis using en masse nuclear run-ons and ribonomic profiling with autoimmune sera. 
  • Granulocyte-macrophage colony-stimulating factor and other cytokines regulate surface expression of the leukocyte adhesion molecule-1 on human neutrophils, monocytes, and their precursors. 
  • Growth arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase 1 and inhibitor 1. 
  • Growth hormone regulates phosphorylation and function of CCAAT/enhancer-binding protein beta by modulating Akt and glycogen synthase kinase-3. 
  • Hepatocyte nuclear factor-4alpha mediates redox sensitivity of inducible nitric-oxide synthase gene transcription. 
  • Hereditary complement (C2) deficiency with dermatomyositis. 
  • Heterogeneity in the B1 (CD20) cell surface molecule expressed by human B-lymphocytes. 
  • High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae. 
  • High-level synthesis of biologically active reovirus protein sigma 1 in a mammalian expression vector system. 
  • Human antigen-specific memory T cells express the homing receptor (LAM-1) necessary for lymphocyte recirculation. 
  • Human medullary thymocyte p80 antigen and In(Lu)-related p80 antigen reside on the same protein. 
  • Human placenta makes extracellular glutathione peroxidase and secretes it into maternal circulation. 
  • Humans have antibodies capable of recognizing oncoviral glycoproteins: demonstration that these antibodies are formed in response to cellular modification of glycoproteins rather than as consequence of exposure to virus. 
  • Hyperosmotic stress stimulates promoter activity and regulates cellular utilization of the serum- and glucocorticoid-inducible protein kinase (Sgk) by a p38 MAPK-dependent pathway. 
  • Identification of ENV determinants in V3 that influence the molecular anatomy of CCR5 utilization. 
  • Identification of a motif in the carboxyl terminus of beta -arrestin2 responsible for activation of JNK3. 
  • Identification of the endogenous smooth muscle myosin phosphatase-associated kinase. 
  • Identification of the ligand-binding subunit of the human 5-hydroxytryptamine1A receptor with N-(p-azido-m-[125I] iodophenethyl)spiperone, a high affinity radioiodinated photoaffinity probe. 
  • Immunochemical and molecular analysis of antigen binding to lipid anchored and soluble forms of an MHC independent human alpha/beta T cell receptor. 
  • Inhibition of 1,25-dihydroxyvitamin D3-dependent transcription by synthetic LXXLL peptide antagonists that target the activation domains of the vitamin D and retinoid X receptors. 
  • Inhibition of nuclear factor-kappaB-mediated adhesion molecule expression in human endothelial cells. 
  • Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2. 
  • Inositol 1,4,5-trisphosphate receptor localization and stability in neonatal cardiomyocytes requires interaction with ankyrin-B. 
  • Interaction of estrogen receptor alpha with protein kinase C alpha and c-Src in osteoblasts during differentiation. 
  • Isoform-specific thyroid hormone receptor antibodies detect multiple thyroid hormone receptors in rat and human pituitaries. 
  • Isolation and characterization of the rat chromosomal gene for a polypeptide (pS1) antigenically related to statin. 
  • Isolation of a new RNA polymerase-binding protein from sporulating Bacillus subtilis. 
  • Isolation of the JMH antigen on a novel phosphatidylinositol-linked human membrane protein. 
  • Isolation of the major viral glycoprotein and a putative precursor from cells transformed by avian sarcoma viruses. 
  • Jun dimerization protein 2 functions as a progesterone receptor N-terminal domain coactivator. 
  • Kv3.1b is a novel component of CNS nodes. 
  • Leucine periodicity of U2 small nuclear ribonucleoprotein particle (snRNP) A' protein is implicated in snRNP assembly via protein-protein interactions. 
  • Localization and interaction of NHERF isoforms in the renal proximal tubule of the mouse. 
  • Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. 
  • Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. 
  • Mapping of a ligand-binding site for the human thromboxane A2 receptor protein. 
  • Mapping the ankyrin-binding site of the human erythrocyte anion exchanger. 
  • Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome. 
  • Monocyte adhesion in patients with bone marrow fibrosis is required for the production of fibrogenic cytokines. Potential role for interleukin-1 and TGF-beta. 
  • Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. 
  • Mutational analysis of the conserved basic domain of human immunodeficiency virus tat protein. 
  • Mutational definition of the human immunodeficiency virus type 1 Rev activation domain. 
  • Mutations in the segment polarity genes wingless and porcupine impair secretion of the wingless protein. 
  • Mutations of the serine phosphorylated in the protein phosphatase-1-binding motif in the skeletal muscle glycogen-targeting subunit. 
  • NHERF associations with sodium-hydrogen exchanger isoform 3 (NHE3) and ezrin are essential for cAMP-mediated phosphorylation and inhibition of NHE3. 
  • Neurotrophin-3 mediates the autocrine survival of the catecholaminergic CAD CNS neuronal cell line. 
  • Notch 1 signaling regulates peripheral T cell activation. 
  • Nutritional and antigenic effects of two bovine milk preparations in infants. 
  • O-glycosylation as a sorting determinant for cell surface delivery in yeast. 
  • Osteopontin inhibits expression of cytochrome c oxidase in RAW 264.7 murine macrophages. 
  • Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription. 
  • P150c-abl is detected in mouse male germ cells by an in vitro kinase assay and is associated with stage-specific phosphoproteins in haploid cells. 
  • PINA is essential for growth and positively influences NIMA function in Aspergillus nidulans. 
  • Phosphatidylinositol kinase type I activity associates with various oncogene products. 
  • Phosphorylation of the rev gene product of human immunodeficiency virus type 1. 
  • Phosphorylation selectively increases triiodothyronine receptor homodimer binding to DNA. 
  • Production and characterization of antipeptide antibodies against human O6-methylguanine-DNA methyltransferase. 
  • Production of congenital malformations using tissueantisera. 8. Effectiveness of reduced, alkylated and digested anti-kidney antibodies. 
  • Progesterone receptor interacting coregulatory proteins and cross talk with cell signaling pathways. 
  • Promoter-specific trans-activation by the adenovirus E1A12S product involves separate E1A domains. 
  • Protein phosphatase 2A and its B56 regulatory subunit inhibit Wnt signaling in Xenopus. 
  • Protein tyrosine phosphatase 1B antagonizes signalling by oncoprotein tyrosine kinase p210 bcr-abl in vivo. 
  • RANK, RANKL, OPG, and M-CSF expression in stromal cells during corneal wound healing. 
  • RGS10 is a selective activator of G alpha i GTPase activity. 
  • RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation. 
  • Ras-dependent activation of fibroblast mitogen-activated protein kinase by 5-HT1A receptor via a G protein beta gamma-subunit-initiated pathway. 
  • Receptor interconversion model of hormone action. 2. Requirement of both kinase and phosphatase activities for conferring estrogen binding activity to the estrogen receptor. 
  • Recombinant adenovirus vector expressing wild-type p53 is a potent inhibitor of prostate cancer cell proliferation. 
  • Regulation of E2A activities by histone acetyltransferases in B lymphocyte development. 
  • Regulation of V2 vasopressin receptor degradation by agonist-promoted ubiquitination. 
  • Regulation of arrestin-3 phosphorylation by casein kinase II. 
  • Regulation of casein kinase I epsilon activity by Wnt signaling. 
  • Regulation of cyclin D1/Cdk4 complexes by calcium/calmodulin-dependent protein kinase I. 
  • Regulation of human interleukin-8 receptor A: identification of a phosphorylation site involved in modulating receptor functions. 
  • Regulation of leukocyte adhesion molecule-1 (TQ1, Leu-8) expression and shedding by normal and malignant cells. 
  • Regulation of leukocyte rolling and adhesion to high endothelial venules through the cytoplasmic domain of L-selectin. 
  • Regulation of the Saccharomyces cerevisiae Slt2 kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase. 
  • Regulation of the tyrosine kinase-dependent adhesion pathway in human lymphocytes through CD45. 
  • Relationship of the human erythrocyte Wrb antigen to an interaction between glycophorin A and band 3. 
  • Requirement for Abl kinases in T cell receptor signaling. 
  • Rev and the fate of pre-mRNA in the nucleus: implications for the regulation of RNA processing in eukaryotes. 
  • Reversible cross-linking combined with immunoprecipitation to study RNA-protein interactions in vivo. 
  • Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays. 
  • Role of C-terminal serines in desensitization and phosphorylation of the mouse thromboxane receptor. 
  • Role of decreased levels of lipid phosphate phosphatase-1 in accumulation of lysophosphatidic acid in ovarian cancer. 
  • Roles of 3,5,3'-triiodothyronine and deoxyribonucleic acid binding on thyroid hormone receptor complex formation. 
  • Salt-split human skin substrate for the immunofluorescent screening of serum from patients with cicatricial pemphigoid and a new method of immunoprecipitation with IgA antibodies. 
  • Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold. 
  • Screening for nitric oxide-dependent protein-protein interactions. 
  • Selective activation of mitogen-activated protein (MAP) kinase kinase 3 and p38alpha MAP kinase is essential for cyclic AMP-dependent UCP1 expression in adipocytes. 
  • Small ubiquitin-related modifier-1 modification mediates resolution of CREB-dependent responses to hypoxia. 
  • Statin, a protein specifically present in nonproliferating cells, is a phosphoprotein and forms a complex with a 45-kilodalton serine/threonine kinase. 
  • Structure-function analyses of the HTLV-I Rex and HIV-1 Rev RNA response elements: insights into the mechanism of Rex and Rev action. 
  • Synaptic vesicle membrane proteins interact to form a multimeric complex. 
  • Synthesis and secretion of von Willebrand factor and fibronectin in megakaryocytes at different phases of maturation. 
  • Targeting of cyclic AMP degradation to beta 2-adrenergic receptors by beta-arrestins. 
  • Tcf-3 expression and beta-catenin mediated transcriptional activation in aggressive fibromatosis (desmoid tumour). 
  • Terminin (Tp 63/60), a novel cell senescence-related protein, is present in the aging human hippocampus. 
  • The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153. 
  • The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs. 
  • The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. 
  • The betagamma subunit of heterotrimeric G proteins interacts with RACK1 and two other WD repeat proteins. 
  • The circadian regulatory proteins BMAL1 and cryptochromes are substrates of casein kinase Iepsilon. 
  • The fine specificity of anti-La antibodies induced in mice by immunization with recombinant human La autoantigen. 
  • The glucocorticoid hormone signal transduction pathway in mice homozygous for chromosomal deletions causing failure of cell type-specific inducible gene expression. 
  • The retinitis pigmentosa GTPase regulator (RPGR) interacts with novel transport-like proteins in the outer segments of rod photoreceptors. 
  • The role of Alzheimer's disease-related presenilin 1 in intercellular adhesion. 
  • The role of the specificity-determining loop of the integrin beta subunit I-like domain in autonomous expression, association with the alpha subunit, and ligand binding. 
  • The structural polypeptides of equine infections anemia virus. 
  • The tor pathway regulates gene expression by linking nutrient sensing to histone acetylation. 
  • The unique identity of rat hepatoma phenylalanine hydroxylase. 
  • Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination. 
  • Transgenic overexpression of the Ca2+-binding protein S100A1 in the heart leads to increased in vivo myocardial contractile performance. 
  • Truncated forms of the insulin-like growth factor II (IGF-II)/mannose 6-phosphate receptor encompassing the IGF-II binding site: characterization of a point mutation that abolishes IGF-II binding. 
  • Two forms of NF-kappa B1 (p105/p50) in murine macrophages: differential regulation by lipopolysaccharide, interleukin-2, and interferon-gamma. 
  • Ubiquitination-dependent proteolysis of O6-methylguanine-DNA methyltransferase in human and murine tumor cells following inactivation with O6-benzylguanine or 1,3-bis(2-chloroethyl)-1-nitrosourea. 
  • Vasoactive intestinal polypeptide type-1 receptor regulation. Desensitization, phosphorylation, and sequestration. 
  • Wee1-regulated apoptosis mediated by the crk adaptor protein in Xenopus egg extracts. 
  • c-Abl is activated by growth factors and Src family kinases and has a role in the cellular response to PDGF. 
  • cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor. 
  • p38 mitogen-activated protein kinase regulates growth factor-induced mitogenesis of rat pulmonary myofibroblasts. 
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  Keywords of People

  • Berchuck, Andrew, James M. Ingram Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Erickson, Harold Paul, James B. Duke Professor of Cell Biology, Biomedical Engineering
  • Krangel, Michael S., Mary Bernheim Professor of Immunology, Immunology