Protein Denaturation

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  Subject Areas on Research

  • (i, i + 4) Ion pairs stabilize helical peptides derived from smooth muscle caldesmon. 
  • A CORRELATION BETWEEN THE PROTEIN STRUCTURE AND CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE. 
  • A kinetic study of the subunit dissociation and reassembly of rabbit muscle phosphofructokinase. 
  • A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins. 
  • A stable dimer in the pH-induced equilibrium unfolding of the homo-hexameric enzyme 4-oxalocrotonate tautomerase (4-OT). 
  • Activation and cross-talk between Akt, NF-kappaB, and unfolded protein response signaling in 1-LN prostate cancer cells consequent to ligation of cell surface-associated GRP78. 
  • Activation of beef heart mitochondrial adenosine triphosphatase by 2,4-dinitrophenol. 
  • An in vitro transcription system that recapitulates equine infectious anemia virus tat-mediated inhibition of human immunodeficiency virus type 1 Tat activity demonstrates a role for positive transcription elongation factor b and associated proteins in the mechanism of Tat activation. 
  • Backbone dynamics of the monomeric lambda repressor denatured state ensemble under nondenaturing conditions. 
  • Betadoublet: de novo design, synthesis, and characterization of a beta-sandwich protein. 
  • Biological and clinical aspects of hyperthermia in cancer therapy. 
  • Cathepsin B1. A lysosomal enzyme that degrades native collagen. 
  • Chaperones rescue luciferase folding by separating its domains. 
  • Characterization of an iron-sulfur cluster assembly protein (ISU1) from Schizosaccharomyces pombe. 
  • Chemical Denaturation and Protein Precipitation Approach for Discovery and Quantitation of Protein-Drug Interactions. 
  • Comparative analysis of two different amide-to-ester bond mutations in the beta-sheet of 4-oxalocrotonate tautomerase. 
  • Computational design of a Zn2+ receptor that controls bacterial gene expression. 
  • Contribution of a buried hydrogen bond to lambda repressor folding kinetics. 
  • De novo design, expression, and characterization of Felix: a four-helix bundle protein of native-like sequence. 
  • Deletion of cysteine 369 in lysyl hydroxylase 1 eliminates enzyme activity and causes Ehlers-Danlos syndrome type VI. 
  • Denatured muscle as a nerve conduit: a functional, morphologic, and electrophysiologic evaluation. 
  • Direct analysis of backbone-backbone hydrogen bond formation in protein folding transition states. 
  • Effect of conformation on the binding of flavins to flavoenzymes. 
  • Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor. 
  • Effect of high mobility group proteins 14 and 17 on the structural and transcriptional properties of acetylated complete and H2A.H2B-deficient nucleosomal cores. 
  • Engineered chimeric streptavidin tetramers as novel tools for bioseparations and drug delivery. 
  • Fast and faster: a designed variant of the B-domain of protein A folds in 3 microsec. 
  • Fast and forceful refolding of stretched alpha-helical solenoid proteins. 
  • Folding kinetics of a fluorescent variant of monomeric lambda repressor. 
  • Guanidine-induced equilibrium unfolding of a homo-hexameric enzyme 4-oxalocrotonate tautomerase (4-OT). 
  • H/D exchange- and mass spectrometry-based strategy for the thermodynamic analysis of protein-ligand binding. 
  • Heat and cold denatured states of monomeric lambda repressor are thermodynamically and conformationally equivalent. 
  • Identification of new mamu-DRB alleles using DGGE and direct sequencing. 
  • Immunological correspondence between arthropod hemocyanin subunits. II. Xiphosuran (Limulus) and spider (Eurypelma, Cupiennius) hemocyanin. 
  • In vitro biological activities of echinonectin. 
  • Influence of denaturing agents on solvent structure. 
  • Initiation of protein synthesis in animal mitochondria. Purification and characterization of translational initiation factor 2. 
  • Interaction of complex polysaccharides with the complement system: effect of calcium depletion on terminal component consumption. 
  • Interaction of lubricin with type II collagen surfaces: adsorption, friction, and normal forces. 
  • Mapping of immunogenic regions of human T cell leukemia virus type I (HTLV-I) gp46 and gp21 envelope glycoproteins with env-encoded synthetic peptides and a monoclonal antibody to gp46. 
  • Mechanical and chemical unfolding of a single protein: a comparison. 
  • Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering. 
  • Mechanical unfolding of an ankyrin repeat protein. 
  • Mechanical unfolding of ubiquitin molecules. 
  • Mechanism of autophosphorylation of Escherichia coli nitrogen regulator II (NR(II) or NtrB): trans-Phosphorylation between subunits 
  • Methionine oxidation of monomeric lambda repressor: the denatured state ensemble under nondenaturing conditions. 
  • Microglassification™: a novel technique for protein dehydration. 
  • Modulating protein structure with fluorous amino acids: increased stability and native-like structure conferred on a 4-helix bundle protein by hexafluoroleucine. 
  • Mutagenesis studies of substrate recognition and catalysis in the sortase A transpeptidase from Staphylococcus aureus. 
  • N-terminal extension changes the folding mechanism of the FK506-binding protein. 
  • Native fluorescence and mag-indo-1-protein interaction as tools for probing unfolding and refolding sequences of the bovine serum albumin subdomain in the presence of guanidine hydrochloride. 
  • Pathways of folding of reduced bovine pancreatic ribonuclease. 
  • Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain. 
  • Phosphorylation of RNA polymerase II CTD fragments results in tight binding to the WW domain from the yeast prolyl isomerase Ess1. 
  • Point mutations alter the mechanical stability of immunoglobulin modules. 
  • Preorganized secondary structure as an important determinant of fast protein folding. 
  • Presence of glycerol masks the effects of phosphorylation on the catalytic efficiency of cytosolic phospholipase A2. 
  • Probing the folding and unfolding dynamics of secondary and tertiary structures in a three-helix bundle protein. 
  • Protein imperfections: separating intrinsic from extrinsic variation of torsion angles. 
  • Protocol for the thermodynamic analysis of some proteins using an H/D exchange- and mass spectrometry-based technique. 
  • Purification and activity study of the A- and B-chains of cinnamomin, a type II ribosome-inactivating protein. 
  • Purification and characterization of a lecithin-D(-)- -hydroxybutyrate dehydrogenase complex. 
  • Quantification of mouse macrophage chemotaxis in vitro: role of C5 for the production of chemotactic activity. 
  • Quantifying kinetic paths of protein folding. 
  • Quantitative protein stability measurement in vivo. 
  • Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. IV. The Escherichia coli hemoflavoprotein: subunit structure and dissociation into hemoprotein and flavoprotein components. 
  • Relaxation spectrometry of biological systems. 
  • Resonance Raman spectroscopic characterization of the molybdopterin active site of DMSO reductase. 
  • Reversible binding of platelet-derived growth factor-AA, -AB, and -BB isoforms to a similar site on the "slow" and "fast" conformations of alpha 2-macroglobulin. 
  • Reversible unfolding of fibronectin type III and immunoglobulin domains provides the structural basis for stretch and elasticity of titin and fibronectin. 
  • Role of granulocyte collagenase in collagen degradation. 
  • SILAC-pulse proteolysis: A mass spectrometry-based method for discovery and cross-validation in proteome-wide studies of ligand binding. 
  • SP75 is encoded by the DP87 gene and belongs to a family of modular Dictyostelium discoideum outer layer spore coat proteins. 
  • Scanning tunnelling microscopy observation of cytochrome-c denaturation induced by bromopyrogal red on highly oriented pyrolytic graphite. 
  • Single protein misfolding events captured by atomic force microscopy. 
  • Stability of oxidized Escherichia coli thioredoxin and its dependence on protonation of the aspartic acid residue in the 26 position. 
  • Stability studies on a lipase from Bacillus subtilis in guanidinium chloride. 
  • Stretching fibronectin. 
  • Structural adaptations that modulate monosaccharide, disaccharide, and trisaccharide specificities in periplasmic maltose-binding proteins. 
  • Structural analysis of semi-specific oligosaccharide recognition by a cellulose-binding protein of thermotoga maritima reveals adaptations for functional diversification of the oligopeptide periplasmic binding protein fold. 
  • Structural and transcriptional properties of different nucleosomal particles containing high mobility group proteins 14 and 17 (HMG 14/17). 
  • Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase. 
  • Structure and stability of monomeric lambda repressor: NMR evidence for two-state folding. 
  • Studies of the copper and heme cofactors of pseudomonad L-tryptophan-2,3-dioxygenase by electron paramagnetic resonance spectroscopy. 
  • Studies on the dissociation of flavin adenine dinucleotide from metalloflavoproteins. 
  • Submillisecond folding of monomeric lambda repressor. 
  • Subunit dissociation and unfolding of rabbit muscle phosphofructokinase by guanidine by hydrochloride. 
  • Tetramerization and single-stranded DNA binding properties of native and mutated forms of murine mitochondrial single-stranded DNA-binding proteins. 
  • The Mechanism of HdeA Unfolding and Chaperone Activation. 
  • The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites. 
  • The human ACC2 CT-domain C-terminus is required for full functionality and has a novel twist. 
  • The human poliovirus receptor. Receptor-virus interaction and parameters of disease specificity. 
  • The influence of follicle-stimulating hormone on testicular phospholipids. 
  • The principal neutralization determinant of simian immunodeficiency virus differs from that of human immunodeficiency virus type 1. 
  • The relationship of Mo, molybdopterin, and the cyanolyzable sulfur in the Mo cofactor. 
  • Thermodynamic analysis of protein stability and ligand binding using a chemical modification- and mass spectrometry-based strategy. 
  • Thermodynamic analysis of subunit interactions in Escherichia coli molybdopterin synthase. 
  • Thermodynamic characterization of the osmolyte- and ligand-folded states of Bacillus subtilis ribonuclease P protein. 
  • Two proteins of the Dictyostelium spore coat bind to cellulose in vitro. 
  • Ubiquitin-mediated degradation a mechanism for fine-tuning TGF-beta signaling. 
  • Ubiquitination directly enhances activity of the deubiquitinating enzyme ataxin-3. 
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  Keywords of People

  • Bennett, Vann, George B. Geller Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute
  • Erickson, Harold Paul, James B. Duke Professor of Cell Biology, Biomedical Engineering
  • Oas, Terrence Gilbert, Professor of Biochemistry, Duke Cancer Institute