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Subject Areas on Research
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(i, i + 4) Ion pairs stabilize helical peptides derived from smooth muscle caldesmon.
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A CORRELATION BETWEEN THE PROTEIN STRUCTURE AND CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE.
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A kinetic study of the subunit dissociation and reassembly of rabbit muscle phosphofructokinase.
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A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins.
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A stable dimer in the pH-induced equilibrium unfolding of the homo-hexameric enzyme 4-oxalocrotonate tautomerase (4-OT).
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Activation and cross-talk between Akt, NF-kappaB, and unfolded protein response signaling in 1-LN prostate cancer cells consequent to ligation of cell surface-associated GRP78.
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Activation of beef heart mitochondrial adenosine triphosphatase by 2,4-dinitrophenol.
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Alpha-crystallin, a molecular chaperone, forms a stable complex with carbonic anhydrase upon heat denaturation.
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An in vitro transcription system that recapitulates equine infectious anemia virus tat-mediated inhibition of human immunodeficiency virus type 1 Tat activity demonstrates a role for positive transcription elongation factor b and associated proteins in the mechanism of Tat activation.
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Backbone dynamics of the monomeric lambda repressor denatured state ensemble under nondenaturing conditions.
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Betadoublet: de novo design, synthesis, and characterization of a beta-sandwich protein.
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Biological and clinical aspects of hyperthermia in cancer therapy.
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Cathepsin B1. A lysosomal enzyme that degrades native collagen.
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Chaperones rescue luciferase folding by separating its domains.
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Characterization of an iron-sulfur cluster assembly protein (ISU1) from Schizosaccharomyces pombe.
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Chemical Denaturation and Protein Precipitation Approach for Discovery and Quantitation of Protein-Drug Interactions.
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Cold sensitivity of the SARS-CoV-2 spike ectodomain.
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Comparative Analysis of Mass-Spectrometry-Based Proteomic Methods for Protein Target Discovery Using a One-Pot Approach.
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Comparative analysis of two different amide-to-ester bond mutations in the beta-sheet of 4-oxalocrotonate tautomerase.
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Computational design of a Zn2+ receptor that controls bacterial gene expression.
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Contribution of a buried hydrogen bond to lambda repressor folding kinetics.
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De novo design, expression, and characterization of Felix: a four-helix bundle protein of native-like sequence.
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Deletion of cysteine 369 in lysyl hydroxylase 1 eliminates enzyme activity and causes Ehlers-Danlos syndrome type VI.
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Denatured muscle as a nerve conduit: a functional, morphologic, and electrophysiologic evaluation.
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Deoxyribonucleic acid ligase. Isolation and physical characterization of the homogeneous enzyme from Escherichia coli.
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Direct analysis of backbone-backbone hydrogen bond formation in protein folding transition states.
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Effect of conformation on the binding of flavins to flavoenzymes.
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Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor.
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Elastic response, buckling, and instability of microtubules under radial indentation.
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Engineered chimeric streptavidin tetramers as novel tools for bioseparations and drug delivery.
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Fast and faster: a designed variant of the B-domain of protein A folds in 3 microsec.
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Fast and forceful refolding of stretched alpha-helical solenoid proteins.
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Folding kinetics of a fluorescent variant of monomeric lambda repressor.
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Guanidine-induced equilibrium unfolding of a homo-hexameric enzyme 4-oxalocrotonate tautomerase (4-OT).
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H/D exchange- and mass spectrometry-based strategy for the thermodynamic analysis of protein-ligand binding.
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Heat and cold denatured states of monomeric lambda repressor are thermodynamically and conformationally equivalent.
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Identification of new mamu-DRB alleles using DGGE and direct sequencing.
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Immunological correspondence between arthropod hemocyanin subunits. II. Xiphosuran (Limulus) and spider (Eurypelma, Cupiennius) hemocyanin.
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In vitro biological activities of echinonectin.
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Influence of denaturing agents on solvent structure.
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Initiation of protein synthesis in animal mitochondria. Purification and characterization of translational initiation factor 2.
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Interaction of complex polysaccharides with the complement system: effect of calcium depletion on terminal component consumption.
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Interaction of lubricin with type II collagen surfaces: adsorption, friction, and normal forces.
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Mapping of immunogenic regions of human T cell leukemia virus type I (HTLV-I) gp46 and gp21 envelope glycoproteins with env-encoded synthetic peptides and a monoclonal antibody to gp46.
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Mechanical and chemical unfolding of a single protein: a comparison.
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Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering.
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Mechanical unfolding of an ankyrin repeat protein.
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Mechanical unfolding of ubiquitin molecules.
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Mechanism of autophosphorylation of Escherichia coli nitrogen regulator II (NRII or NtrB): trans-phosphorylation between subunits.
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Methionine oxidation of monomeric lambda repressor: the denatured state ensemble under nondenaturing conditions.
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Microglassification™: a novel technique for protein dehydration.
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Modulating protein structure with fluorous amino acids: increased stability and native-like structure conferred on a 4-helix bundle protein by hexafluoroleucine.
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Multilayer adsorption of lysozyme on a hydrophobic substrate.
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Mutagenesis studies of substrate recognition and catalysis in the sortase A transpeptidase from Staphylococcus aureus.
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N-terminal extension changes the folding mechanism of the FK506-binding protein.
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Native fluorescence and mag-indo-1-protein interaction as tools for probing unfolding and refolding sequences of the bovine serum albumin subdomain in the presence of guanidine hydrochloride.
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Pathways of folding of reduced bovine pancreatic ribonuclease.
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Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain.
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Phosphorylation of RNA polymerase II CTD fragments results in tight binding to the WW domain from the yeast prolyl isomerase Ess1.
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Point mutations alter the mechanical stability of immunoglobulin modules.
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Preorganized secondary structure as an important determinant of fast protein folding.
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Probing the folding and unfolding dynamics of secondary and tertiary structures in a three-helix bundle protein.
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Probing the oligomeric structure of an enzyme by electrospray ionization time-of-flight mass spectrometry.
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Protein imperfections: separating intrinsic from extrinsic variation of torsion angles.
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Protocol for the thermodynamic analysis of some proteins using an H/D exchange- and mass spectrometry-based technique.
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Purification and activity study of the A- and B-chains of cinnamomin, a type II ribosome-inactivating protein.
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Purification and characterization of a lecithin-D(-)- -hydroxybutyrate dehydrogenase complex.
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Quantification of mouse macrophage chemotaxis in vitro: role of C5 for the production of chemotactic activity.
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Quantifying kinetic paths of protein folding.
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Quantitative protein stability measurement in vivo.
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Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. IV. The Escherichia coli hemoflavoprotein: subunit structure and dissociation into hemoprotein and flavoprotein components.
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Relaxation spectrometry of biological systems.
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Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle.
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Resonance Raman spectroscopic characterization of the molybdopterin active site of DMSO reductase.
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Reversible binding of platelet-derived growth factor-AA, -AB, and -BB isoforms to a similar site on the "slow" and "fast" conformations of alpha 2-macroglobulin.
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Reversible unfolding of fibronectin type III and immunoglobulin domains provides the structural basis for stretch and elasticity of titin and fibronectin.
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Role of aspartic acid 38 in the cofactor specificity of Drosophila alcohol dehydrogenase.
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Role of granulocyte collagenase in collagen degradation.
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SILAC-pulse proteolysis: A mass spectrometry-based method for discovery and cross-validation in proteome-wide studies of ligand binding.
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SP75 is encoded by the DP87 gene and belongs to a family of modular Dictyostelium discoideum outer layer spore coat proteins.
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Scanning tunnelling microscopy observation of cytochrome-c denaturation induced by bromopyrogal red on highly oriented pyrolytic graphite.
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Single protein misfolding events captured by atomic force microscopy.
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Site-directed mutagenesis of glycine-14 and two "critical" cysteinyl residues in Drosophila alcohol dehydrogenase.
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Stability of oxidized Escherichia coli thioredoxin and its dependence on protonation of the aspartic acid residue in the 26 position.
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Stability studies on a lipase from Bacillus subtilis in guanidinium chloride.
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Stretching fibronectin.
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Structural adaptations that modulate monosaccharide, disaccharide, and trisaccharide specificities in periplasmic maltose-binding proteins.
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Structural analysis of semi-specific oligosaccharide recognition by a cellulose-binding protein of thermotoga maritima reveals adaptations for functional diversification of the oligopeptide periplasmic binding protein fold.
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Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase.
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Structure and stability of monomeric lambda repressor: NMR evidence for two-state folding.
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Studies of the copper and heme cofactors of pseudomonad L-tryptophan-2,3-dioxygenase by electron paramagnetic resonance spectroscopy.
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Studies on the dissociation of flavin adenine dinucleotide from metalloflavoproteins.
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Submillisecond folding of monomeric lambda repressor.
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Subunit dissociation and unfolding of rabbit muscle phosphofructokinase by guanidine by hydrochloride.
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Tetramerization and single-stranded DNA binding properties of native and mutated forms of murine mitochondrial single-stranded DNA-binding proteins.
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The Mechanism of HdeA Unfolding and Chaperone Activation.
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The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites.
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The human ACC2 CT-domain C-terminus is required for full functionality and has a novel twist.
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The human poliovirus receptor. Receptor-virus interaction and parameters of disease specificity.
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The influence of follicle-stimulating hormone on testicular phospholipids.
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The principal neutralization determinant of simian immunodeficiency virus differs from that of human immunodeficiency virus type 1.
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The relationship of Mo, molybdopterin, and the cyanolyzable sulfur in the Mo cofactor.
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Thermodynamic analysis of protein stability and ligand binding using a chemical modification- and mass spectrometry-based strategy.
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Thermodynamic analysis of subunit interactions in Escherichia coli molybdopterin synthase.
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Thermodynamic characterization of the osmolyte- and ligand-folded states of Bacillus subtilis ribonuclease P protein.
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Time-resolved, light scattering measurements of cartilage and cornea denaturation due to free electron laser radiation.
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Two proteins of the Dictyostelium spore coat bind to cellulose in vitro.
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Ubiquitin-mediated degradation a mechanism for fine-tuning TGF-beta signaling.
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Ubiquitination directly enhances activity of the deubiquitinating enzyme ataxin-3.
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Keywords of People