Ribonucleoproteins

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  Subject Areas on Research

  • 60-kDa Ro protein autoepitopes identified using recombinant polypeptides. 
  • A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein. 
  • A human autoimmune protein associated with U1 RNA contains a region of homology that is cross-reactive with retroviral p30gag antigen. 
  • A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein. 
  • A novel isoform ratio switch of the polypyrimidine tract binding protein. 
  • A small yeast RNA blocks hepatitis C virus internal ribosome entry site (HCV IRES)-mediated translation and inhibits replication of a chimeric poliovirus under translational control of the HCV IRES element. 
  • A specific 31-nucleotide domain of U1 RNA directly interacts with the 70K small nuclear ribonucleoprotein component. 
  • A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo. 
  • Acute myeloid leukemia ontogeny is defined by distinct somatic mutations. 
  • An ATP-independent U2 small nuclear ribonucleoprotein particle/precursor mRNA complex requires both splice sites and the polypyrimidine tract. 
  • Analysis of autoantibody binding to different regions of the human La antigen expressed in recombinant fusion proteins. 
  • Anti-La antibodies. 
  • Anti-La antibody production by MRL-1pr/1pr mice. Analysis of fine specificity. 
  • Antisense-mediated affinity purification of dengue virus ribonucleoprotein complexes from infected cells. 
  • Binding sites of the 9- and 14-kilodalton heterodimeric protein subunit of the signal recognition particle (SRP) are contained exclusively in the Alu domain of SRP RNA and contain a sequence motif that is conserved in evolution. 
  • Biochemical Methods To Investigate lncRNA and the Influence of lncRNA:Protein Complexes on Chromatin. 
  • Biochemical fractionation and assembly of the membrane components that mediate nascent chain targeting and translocation. 
  • Calreticulin displays in vivo peptide-binding activity and can elicit CTL responses against bound peptides. 
  • Cellular and viral splicing factors can modify the splicing pattern of CFTR transcripts carrying splicing mutations. 
  • Chaperone-mediated cross-priming: a hitchhiker's guide to vesicle transport (review). 
  • Characterization and idiotypic analysis of an anti-RNP monoclonal antibody. 
  • Characterization of a Drosophila U snRNP RNA-binding protein. 
  • Characterization of cDNAs encoding the polypyrimidine tract-binding protein. 
  • Cloning of human and mouse brain cDNAs coding for S1, the second member of the mammalian elongation factor-1 alpha gene family: analysis of a possible evolutionary pathway. 
  • Connections between the processing and nuclear export of mRNA: evidence for an export license? 
  • Coordinated posttranscriptional mRNA population dynamics during T-cell activation. 
  • Development of autoantibodies before the clinical onset of systemic lupus erythematosus. 
  • Direct interactions between autoantigen La and human immunodeficiency virus leader RNA. 
  • Disruption of mRNA-RNP formation and sorting to dendritic synapses by antisense oligodeoxynucleotides. 
  • ELAV protein HuA (HuR) can redistribute between nucleus and cytoplasm and is upregulated during serum stimulation and T cell activation. 
  • ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system. 
  • ELAV tumor antigen, Hel-N1, increases translation of neurofilament M mRNA and induces formation of neurites in human teratocarcinoma cells. 
  • Early events in lupus humoral autoimmunity suggest initiation through molecular mimicry. 
  • Embryonic lethal abnormal visual RNA-binding proteins involved in growth, differentiation, and posttranscriptional gene expression. 
  • Epitope specificity of anti-La antibodies from patients with Sjögren's syndrome. 
  • Eukaryotic mRNPs may represent posttranscriptional operons. 
  • Expression of autoantibodies to recombinant (U1) RNP-associated 70K antigen in systemic lupus erythematosus. 
  • Gene expression analysis of messenger RNP complexes. 
  • Genome-wide regulatory analysis using en masse nuclear run-ons and ribonomic profiling with autoimmune sera. 
  • Genomic structure and amino acid sequence domains of the human La autoantigen. 
  • Hel-N1/Hel-N2 proteins are bound to poly(A)+ mRNA in granular RNP structures and are implicated in neuronal differentiation. 
  • Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. 
  • Hybridoma SLE autoantibodies: insights for the pathogenesis of autoimmune disease. 
  • Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. 
  • Identification of casein kinase II and phosphorylated proteins associated with messenger ribonucleoproteins particles from reticulocytes. 
  • Identification of cis elements directing termination of yeast nonpolyadenylated snoRNA transcripts. 
  • Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays. 
  • Immunization with the Sm nuclear antigen induces anti-Sm antibodies in normal and MRL mice. 
  • Independent expression of autoantibodies in systemic lupus erythematosus. 
  • La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate. 
  • Leucine periodicity of U2 small nuclear ribonucleoprotein particle (snRNP) A' protein is implicated in snRNP assembly via protein-protein interactions. 
  • Linked folding and anion binding of the Bacillus subtilis ribonuclease P protein. 
  • Localization of S1 and elongation factor-1 alpha mRNA in rat brain and liver by non-radioactive in situ hybridization. 
  • Lymphocytic lymphoma and systemic lupus erythematosus: their coexistence with antibody to the Sm antigen. 
  • Mammalian homologs of Drosophila ELAV localized to a neuronal subset can bind in vitro to the 3' UTR of mRNA encoding the Id transcriptional repressor. 
  • Mechanisms coordinating ELAV/Hu mRNA regulons. 
  • Messenger ribonucleoprotein complexes containing human ELAV proteins: interactions with cytoskeleton and translational apparatus. 
  • Molecular analysis of the 60-kDa human Ro ribonucleoprotein. 
  • Molecular biology of nuclear autoantigens. 
  • Molecular composition of Ro small ribonucleoprotein complexes in human cells. Intracellular localization of the 60- and 52-kD proteins. 
  • Molecular structure of the La and Ro autoantigens and their use in autoimmune diagnostics. 
  • Mouse models of Casc3 reveal developmental functions distinct from other components of the exon junction complex. 
  • Multiple single-stranded cis elements are associated with activated chromatin of the human c-myc gene in vivo. 
  • Nuclear import of hnRNP A1 is mediated by a novel cellular cofactor related to karyopherin-beta. 
  • Nuclear spreads: I. Visualization of bipartite ribosomal RNA domains. 
  • Nucleotide sequence and host La protein interactions of rabies virus leader RNA. 
  • Organizing mRNA export. 
  • Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche. 
  • Podocyte cell cycle regulation and proliferation in collapsing glomerulopathies. 
  • Polypyrimidine tract binding protein antagonizes exon definition. 
  • Proteomic analysis of mitotic RNA polymerase II reveals novel interactors and association with proteins dysfunctional in disease. 
  • Purification of messenger ribonucleoprotein particles from rabbit reticulocytes by zonal centrifugation in metrizamide. 
  • Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA. 
  • Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen. 
  • RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts. 
  • RNA binding by Sxl proteins in vitro and in vivo. 
  • RNA binding specificity of a Drosophila snRNP protein that shares sequence homology with mammalian U1-A and U2-B" proteins. 
  • RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins. 
  • Rapid inhibition of processing and assembly of small nuclear ribonucleoproteins after infection with vesicular stomatitis virus. 
  • Recognition of U1 and U2 small nuclear RNAs can be altered by a 5-amino-acid segment in the U2 small nuclear ribonucleoprotein particle (snRNP) B" protein and through interactions with U2 snRNP-A' protein. 
  • Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays. 
  • Ribonucleoprotein infrastructure regulating the flow of genetic information between the genome and the proteome. 
  • Role of chaperones in antigen processing. 
  • SR proteins escort the U4/U6.U5 tri-snRNP to the spliceosome. 
  • Sequences within a small yeast RNA required for inhibition of internal initiation of translation: interaction with La and other cellular proteins influences its inhibitory activity. 
  • Specificity of autoantibodies for recombinant 60-kd and 52-kd Ro autoantigens. 
  • Structure and morphogenesis of type-C retroviruses. 
  • Systematic analysis of posttranscriptional gene expression. 
  • Temporal correlation of antibody responses to different epitopes of the human La autoantigen. 
  • The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the nuclear RNase P. 
  • The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs. 
  • The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. 
  • The fine specificity of anti-La antibodies induced in mice by immunization with recombinant human La autoantigen. 
  • The fine specificity of monoclonal anti-DNA antibodies induced in normal mice by immunization with bacterial DNA. 
  • The genetics of autoantibody production in MRL/lpr lupus mice. 
  • The origins of defective interfering particles of the negative-strand RNA viruses. 
  • The ribonome: a dominant force in co-ordinating gene expression. 
  • The suppression of testis-brain RNA binding protein and kinesin heavy chain disrupts mRNA sorting in dendrites. 
  • U1-snRNP-A protein selects a ten nucleotide consensus sequence from a degenerate RNA pool presented in various structural contexts. 
  • Unrestraining genetic processes with a protein-DNA hinge. 
  • Viral transcription is necessary and sufficient for vesicular stomatitis virus to inhibit maturation of small nuclear ribonucleoproteins. 
  • Why is Hu where? Shuttling of early-response-gene messenger RNA subsets. 
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  Keywords of People

  • Oas, Terrence Gilbert, Professor of Biochemistry, with tenure, Duke Cancer Institute