DNA-Directed RNA Polymerases

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  Subject Areas on Research

  • A modified procedure for fast purification of T7 RNA polymerase. 
  • A note on RNA polymerase phylogeny. 
  • A specific internal RNA polymerase recognition site of VSV RNA is involved in the generation of DI particles. 
  • A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein. 
  • Activation of the Pseudomonas aeruginosa lasI gene by LasR and the Pseudomonas autoinducer PAI: an autoinduction regulatory hierarchy. 
  • Alpha-amanitin-resistant D. melanogaster with an altered RNA polymerase II. 
  • Antitermination by bacteriophage lambda Q protein. 
  • Appearance of a ribonucleic acid polymerase-binding protein in asporogenous mutants of Bacillus subtilis. 
  • Association of the viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids. 
  • Breaking symmetry in multimeric ATPase motors. 
  • COCO-CL: hierarchical clustering of homology relations based on evolutionary correlations. 
  • Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells. 
  • Characterization of elongating T7 and SP6 RNA polymerases and their response to a roadblock generated by a site-specific DNA binding protein. 
  • Co-evolutionary analysis of domains in interacting proteins reveals insights into domain-domain interactions mediating protein-protein interactions. 
  • Computation, prediction, and experimental tests of fitness for bacteriophage T7 mutants with permuted genomes. 
  • Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase. 
  • Cyclophilin B escorts the hepatitis C virus RNA polymerase: a viral achilles heel? 
  • Decoding the role of regulatory element polymorphisms in complex disease. 
  • Detection of DNA damage in transcriptionally active genes by RT-PCR and assessment of repair of cisplatin-induced damage in the glutathione S-transferase-pi gene in human glioblastoma cells. 
  • Direct carrier testing in 14 families with haemophilia B. 
  • Dissection of the molecular circuitry controlling virulence in Francisella tularensis. 
  • Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence. 
  • Downregulation of RNA polymerase III transcription of the hY3 gene in vitro. 
  • Effects of cyclic adenosine 3': 5'-monophosphate on phosphoprotein kinase and phosphatase fractions prepared from rat liver nuclei. 
  • Elongation by Escherichia coli RNA polymerase is blocked in vitro by a site-specific DNA binding protein. 
  • Emergent bistability by a growth-modulating positive feedback circuit. 
  • Endosymbiont gene functions impaired and rescued by polymerase infidelity at poly(A) tracts. 
  • Engagement of arginine finger to ATP triggers large conformational changes in NtrC1 AAA+ ATPase for remodeling bacterial RNA polymerase. 
  • Eukaryotic gene transcription with purified components. 
  • Female steroid hormones and target cell nuclei. 
  • Fine structure mapping and phenotypic analysis of five temperature-sensitive mutations in the second largest subunit of vaccinia virus DNA-dependent RNA polymerase. 
  • Follicle stimulating hormone regulation of protein kinase activity and protein synthesis in testis. 
  • Functional organization of polytene chromosomes. 
  • Genetic and biochemical characterization of mutants at an RNA polymerase II locus in D. melanogaster. 
  • Genomic amplification with transcript sequencing. 
  • Genotypic diversity of coagulase-negative staphylococci causing endocarditis: a global perspective. 
  • Hormonal control of oviduct growth and differentiation. 
  • Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species. 
  • Identification, molecular cloning, and mutagenesis of Saccharomyces cerevisiae RNA polymerase genes. 
  • Immunological studies of RNA polymerase II using antibodies to subunits of Drosophila and wheat germ enzyme. 
  • In vitro coupled transcription splicing. 
  • In vitro proteolysis of a large subunit of Drosophila melanogaster RNA polymerase B. 
  • In vitro selection of aptamers from RNA libraries. 
  • In vitro synthesis of messenger RNA by a defective interfering particle of vesicular stomatitis virus. 
  • Incorporation of ribonucleoside 5'-(alpha-P-borano)triphosphates into a 20-mer RNA by T7 RNA polymerase. 
  • Interaction with RNA polymerase of nucleosomal cores lacking one H2A.H2B dimer. 
  • Isolation of a new RNA polymerase-binding protein from sporulating Bacillus subtilis. 
  • Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase. 
  • Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster. 
  • Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. 
  • Mechanisms of chromium-induced suppression of RNA synthesis in cellular and cell-free systems: relationship to RNA polymerase arrest. 
  • Molecular cloning of sequences from a Drosophila RNA polymerase II locus by P element transposon tagging. 
  • Nuclear estrogen receptor in the avian liver: correlation with biologic response. 
  • Nucleotide-induced asymmetry within ATPase activator ring drives σ54-RNAP interaction and ATP hydrolysis. 
  • Outer membrane vesicle production by Escherichia coli is independent of membrane instability. 
  • Properties of mutationally altered RNA polymerases II of Drosophila. 
  • Purification of the three nuclear RNA polymerases from Neurospora crassa. 
  • Purified MotA protein binds the -30 region of a bacteriophage T4 middle-mode promoter and activates transcription in vitro. 
  • RNA polymerase B (or II) in heat induced puffs of Drosophila polytene chromosomes. 
  • RNA polymerase B from Drosophila melanogaster larvae. Purification and partial characterization. 
  • RNA polymerase from sporulating Bacillus subtilis. Purification and properties of a modified form of the enzyme containing two sporulation polypeptides. 
  • RNA polymerase-associated interactions near template promoter sequences of defective interfering particles of vesicular stomatitis virus. 
  • Rapid molecular detection of tuberculosis and rifampin resistance. 
  • Reading, writing, and modulating genetic information with boranophosphate mimics of nucleotides, DNA, and RNA. 
  • Rotavirus RNA polymerase requires the core shell protein to synthesize the double-stranded RNA genome. 
  • Sequential activation of splenic nuclear RNA polymerases by erythropoietin. 
  • Simultaneous genotyping and species identification using hybridization pattern recognition analysis of generic Mycobacterium DNA arrays. 
  • Site-specific RNA cleavage generates the 3' end of a poxvirus late mRNA. 
  • Structural and Biochemical Characterization of the Francisella tularensis Pathogenicity Regulator, Macrophage Locus Protein A (MglA). 
  • Structural studies on Neurospora RNA polymerases and associated proteins. 
  • Structure and evolution of prokaryotic and eukaryotic RNA polymerases: a model. 
  • Substitutions in bacteriophage T4 AsiA and Escherichia coli sigma(70) that suppress T4 motA activation mutations. 
  • Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells. 
  • The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase. 
  • The second-largest subunit of the poxvirus RNA polymerase is similar to the corresponding subunits of procaryotic and eucaryotic RNA polymerases. 
  • Transcription of bacteriophage fl. The major in vivo RNAs. 
  • Transcription of class III genes: formation of preinitiation complexes. 
  • Transcriptional pausing in a region important for plasmid NR1 replication control. 
  • Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome.