RNA Precursors
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Subject Areas on Research
- 5' exon replacement and repair by spliceosome-mediated RNA trans-splicing.
- A compensatory base change in human U2 snRNA can suppress a branch site mutation.
- A mammalian activity required for the second step of pre-messenger RNA splicing.
- A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.
- A mutational analysis of the polypyrimidine tract of introns. Effects of sequence differences in pyrimidine tracts on splicing.
- A novel assay for viral microRNA function identifies a single nucleotide polymorphism that affects Drosha processing.
- A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding.
- A single nucleotide linked to a switch in metal ion reactivity preference in the HDV ribozymes.
- A stem structure in fibroblast growth factor receptor 2 transcripts mediates cell-type-specific splicing by approximating intronic control elements.
- Alternative Splicing of EZH2 pre-mRNA by SF3B3 Contributes to the Tumorigenic Potential of Renal Cancer.
- Alternative splicing: therapeutic target and tool.
- An ATP-independent U2 small nuclear ribonucleoprotein particle/precursor mRNA complex requires both splice sites and the polypyrimidine tract.
- An intronic sequence element mediates both activation and repression of rat fibroblast growth factor receptor 2 pre-mRNA splicing.
- An intronic splicing silencer causes skipping of the IIIb exon of fibroblast growth factor receptor 2 through involvement of polypyrimidine tract binding protein.
- Ankyrin regulation: an alternatively spliced segment of the regulatory domain functions as an intramolecular modulator.
- Assaying nuclear messenger RNA export in human cells.
- Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro.
- Characterization of cDNAs encoding the polypyrimidine tract-binding protein.
- Co-transcriptional splicing of pre-messenger RNAs: considerations for the mechanism of alternative splicing.
- Connections between the processing and nuclear export of mRNA: evidence for an export license?
- Coupled in vitro synthesis and splicing of RNA polymerase II transcripts.
- Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex.
- Dynamic microRNA gene transcription and processing during T cell development.
- Early history of circular RNAs, children of splicing.
- Evaluating group I intron catalytic efficiency in mammalian cells.
- Exon circularization in mammalian nuclear extracts.
- Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs.
- Fox-2 mediates epithelial cell-specific fibroblast growth factor receptor 2 exon choice.
- Functional conservation of the human homolog of the yeast pre-mRNA splicing factor Prp17p.
- Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein.
- Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing.
- Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage.
- Group I intron self-splicing with adenosine: evidence for a single nucleoside-binding site.
- Group I permuted intron-exon (PIE) sequences self-splice to produce circular exons.
- Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs.
- Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
- Identification of a DNA Damage-Induced Alternative Splicing Pathway That Regulates p53 and Cellular Senescence Markers.
- Identification of cis elements directing termination of yeast nonpolyadenylated snoRNA transcripts.
- Identification of cis-acting elements and splicing factors involved in the regulation of BIM Pre-mRNA splicing.
- Imaging alternative splicing in living cells.
- In vitro coupled transcription splicing.
- Integrative regulatory mapping indicates that the RNA-binding protein HuR couples pre-mRNA processing and mRNA stability.
- Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II.
- Making antisense of splicing.
- Phospho-carboxyl-terminal domain binding and the role of a prolyl isomerase in pre-mRNA 3'-End formation.
- Positive patches and negative noodles: linking RNA processing to transcription?
- Prespliceosome and spliceosome isolation and analysis.
- Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors.
- Proximity-dependent and proximity-independent trans-splicing in mammalian cells.
- RNA as an RNA polymerase: net elongation of an RNA primer catalyzed by the Tetrahymena ribozyme.
- RNA binding by Sxl proteins in vitro and in vivo.
- Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha.
- Recognition and cleavage of primary microRNA transcripts.
- Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing.
- Rescuing dicer defects via inhibition of an anti-dicing nuclease.
- Rev and the fate of pre-mRNA in the nucleus: implications for the regulation of RNA processing in eukaryotes.
- Sequence information for the splicing of human pre-mRNA identified by support vector machine classification.
- Sequence requirements for micro RNA processing and function in human cells.
- Structural plasticity and Mg2+ binding properties of RNase P P4 from combined analysis of NMR residual dipolar couplings and motionally decoupled spin relaxation.
- Structural requirements for pre-microRNA binding and nuclear export by Exportin 5.
- Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei.
- The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p.
- The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function.
- The conserved dinucleotide AG of the 3' splice site may be recognized twice during in vitro splicing of mammalian mRNA precursors.
- The imprinted H19 noncoding RNA is a primary microRNA precursor.
- The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.
- The poly(A) site sequence in HDV RNA alters both extent and rate of self-cleavage of the antigenomic ribozyme.
- The transcription elongation factor CA150 interacts with RNA polymerase II and the pre-mRNA splicing factor SF1.
- Trans-splicing into highly abundant albumin transcripts for production of therapeutic proteins in vivo.
- Transcription and processing of human microRNA precursors.
- Transcriptional origin of Kaposi's sarcoma-associated herpesvirus microRNAs.
- U1 snRNP-ASF/SF2 interaction and 5' splice site recognition: characterization of required elements.
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Keywords of People
- Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
- Ciofani, Maria, Associate Professor of Immunology, Immunology