RNA Precursors

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  Subject Areas on Research

  • 5' exon replacement and repair by spliceosome-mediated RNA trans-splicing. 
  • A compensatory base change in human U2 snRNA can suppress a branch site mutation. 
  • A mammalian activity required for the second step of pre-messenger RNA splicing. 
  • A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein. 
  • A mutational analysis of the polypyrimidine tract of introns. Effects of sequence differences in pyrimidine tracts on splicing. 
  • A novel assay for viral microRNA function identifies a single nucleotide polymorphism that affects Drosha processing. 
  • A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding. 
  • A single nucleotide linked to a switch in metal ion reactivity preference in the HDV ribozymes. 
  • A stem structure in fibroblast growth factor receptor 2 transcripts mediates cell-type-specific splicing by approximating intronic control elements. 
  • Alternative Splicing of EZH2 pre-mRNA by SF3B3 Contributes to the Tumorigenic Potential of Renal Cancer. 
  • Alternative splicing: therapeutic target and tool. 
  • An ATP-independent U2 small nuclear ribonucleoprotein particle/precursor mRNA complex requires both splice sites and the polypyrimidine tract. 
  • An intronic sequence element mediates both activation and repression of rat fibroblast growth factor receptor 2 pre-mRNA splicing. 
  • An intronic splicing silencer causes skipping of the IIIb exon of fibroblast growth factor receptor 2 through involvement of polypyrimidine tract binding protein. 
  • Ankyrin regulation: an alternatively spliced segment of the regulatory domain functions as an intramolecular modulator. 
  • Assaying nuclear messenger RNA export in human cells. 
  • Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro. 
  • Characterization of cDNAs encoding the polypyrimidine tract-binding protein. 
  • Co-transcriptional splicing of pre-messenger RNAs: considerations for the mechanism of alternative splicing. 
  • Connections between the processing and nuclear export of mRNA: evidence for an export license? 
  • Coupled in vitro synthesis and splicing of RNA polymerase II transcripts. 
  • Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex. 
  • Dynamic microRNA gene transcription and processing during T cell development. 
  • Early history of circular RNAs, children of splicing. 
  • Evaluating group I intron catalytic efficiency in mammalian cells. 
  • Exon circularization in mammalian nuclear extracts. 
  • Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. 
  • Fox-2 mediates epithelial cell-specific fibroblast growth factor receptor 2 exon choice. 
  • Functional conservation of the human homolog of the yeast pre-mRNA splicing factor Prp17p. 
  • Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein. 
  • Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing. 
  • Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. 
  • Group I intron self-splicing with adenosine: evidence for a single nucleoside-binding site. 
  • Group I permuted intron-exon (PIE) sequences self-splice to produce circular exons. 
  • Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. 
  • Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. 
  • Identification of a DNA Damage-Induced Alternative Splicing Pathway That Regulates p53 and Cellular Senescence Markers. 
  • Identification of cis elements directing termination of yeast nonpolyadenylated snoRNA transcripts. 
  • Identification of cis-acting elements and splicing factors involved in the regulation of BIM Pre-mRNA splicing. 
  • Imaging alternative splicing in living cells. 
  • In vitro coupled transcription splicing. 
  • Integrative regulatory mapping indicates that the RNA-binding protein HuR couples pre-mRNA processing and mRNA stability. 
  • Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. 
  • Making antisense of splicing. 
  • Phospho-carboxyl-terminal domain binding and the role of a prolyl isomerase in pre-mRNA 3'-End formation. 
  • Positive patches and negative noodles: linking RNA processing to transcription? 
  • Prespliceosome and spliceosome isolation and analysis. 
  • Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors. 
  • Proximity-dependent and proximity-independent trans-splicing in mammalian cells. 
  • RNA as an RNA polymerase: net elongation of an RNA primer catalyzed by the Tetrahymena ribozyme. 
  • RNA binding by Sxl proteins in vitro and in vivo. 
  • Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha. 
  • Recognition and cleavage of primary microRNA transcripts. 
  • Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing. 
  • Rescuing dicer defects via inhibition of an anti-dicing nuclease. 
  • Rev and the fate of pre-mRNA in the nucleus: implications for the regulation of RNA processing in eukaryotes. 
  • Sequence requirements for micro RNA processing and function in human cells. 
  • Structural plasticity and Mg2+ binding properties of RNase P P4 from combined analysis of NMR residual dipolar couplings and motionally decoupled spin relaxation. 
  • Structural requirements for pre-microRNA binding and nuclear export by Exportin 5. 
  • Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei. 
  • The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p. 
  • The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function. 
  • The conserved dinucleotide AG of the 3' splice site may be recognized twice during in vitro splicing of mammalian mRNA precursors. 
  • The imprinted H19 noncoding RNA is a primary microRNA precursor. 
  • The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA. 
  • The poly(A) site sequence in HDV RNA alters both extent and rate of self-cleavage of the antigenomic ribozyme. 
  • The transcription elongation factor CA150 interacts with RNA polymerase II and the pre-mRNA splicing factor SF1. 
  • Trans-splicing into highly abundant albumin transcripts for production of therapeutic proteins in vivo. 
  • Transcription and processing of human microRNA precursors. 
  • Transcriptional origin of Kaposi's sarcoma-associated herpesvirus microRNAs. 
  • U1 snRNP-ASF/SF2 interaction and 5' splice site recognition: characterization of required elements. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Ciofani, Maria, Associate Professor of Immunology, Immunology