RNA Precursors

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  Subject Areas on Research

  • 5' exon replacement and repair by spliceosome-mediated RNA trans-splicing. 
  • A compensatory base change in human U2 snRNA can suppress a branch site mutation. 
  • A single nucleotide linked to a switch in metal ion reactivity preference in the HDV ribozymes. 
  • Alternative Splicing of EZH2 pre-mRNA by SF3B3 Contributes to the Tumorigenic Potential of Renal Cancer. 
  • Ankyrin regulation: an alternatively spliced segment of the regulatory domain functions as an intramolecular modulator. 
  • Co-transcriptional splicing of pre-messenger RNAs: considerations for the mechanism of alternative splicing. 
  • Comparative analysis of LIN28-RNA binding sites identified at single nucleotide resolution. 
  • Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex. 
  • Difference between mitochondrial RNase P and nuclear RNase P. 
  • Dynamic microRNA gene transcription and processing during T cell development. 
  • Evaluating group I intron catalytic efficiency in mammalian cells. 
  • Exon circularization in mammalian nuclear extracts. 
  • Exploiting pre-rRNA processing in Diamond Blackfan anemia gene discovery and diagnosis. 
  • Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein. 
  • Generation of nuclease resistant circular RNA decoys for HIV-Tat and HIV-Rev by autocatalytic splicing. 
  • Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. 
  • Group I intron self-splicing with adenosine: evidence for a single nucleoside-binding site. 
  • Group I permuted intron-exon (PIE) sequences self-splice to produce circular exons. 
  • Identification of a DNA Damage-Induced Alternative Splicing Pathway That Regulates p53 and Cellular Senescence Markers. 
  • Identification of cis elements directing termination of yeast nonpolyadenylated snoRNA transcripts. 
  • Identification of cis-acting elements and splicing factors involved in the regulation of BIM Pre-mRNA splicing. 
  • Integrative regulatory mapping indicates that the RNA-binding protein HuR couples pre-mRNA processing and mRNA stability. 
  • Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. 
  • PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII. 
  • Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced Alternative Splicing Pathway. 
  • Phospho-carboxyl-terminal domain binding and the role of a prolyl isomerase in pre-mRNA 3'-End formation. 
  • Positive patches and negative noodles: linking RNA processing to transcription? 
  • Proximity-dependent and proximity-independent trans-splicing in mammalian cells. 
  • RNA as an RNA polymerase: net elongation of an RNA primer catalyzed by the Tetrahymena ribozyme. 
  • Regulation of Co-transcriptional Pre-mRNA Splicing by m⁶ A through the Low-Complexity Protein hnRNPG. 
  • Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing. 
  • Structural plasticity and Mg2+ binding properties of RNase P P4 from combined analysis of NMR residual dipolar couplings and motionally decoupled spin relaxation. 
  • Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei. 
  • The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p. 
  • The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the nuclear RNase P. 
  • The conserved dinucleotide AG of the 3' splice site may be recognized twice during in vitro splicing of mammalian mRNA precursors. 
  • The deafness-associated mitochondrial DNA mutation at position 7445, which affects tRNASer(UCN) precursor processing, has long-range effects on NADH dehydrogenase subunit ND6 gene expression. 
  • The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA. 
  • The poly(A) site sequence in HDV RNA alters both extent and rate of self-cleavage of the antigenomic ribozyme. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Ciofani, Maria, Associate Professor of Integrative Immunobiology, Cell Biology