Sepharose

• 

  Subject Areas on Research

  • A laminin and nerve growth factor-laden three-dimensional scaffold for enhanced neurite extension. 
  • A standardized approach to PCR-based semiquantitation of multiple cytokine gene transcripts from small cell samples. 
  • ATP induces Ca(2+) signaling in human chondrons cultured in three-dimensional agarose films. 
  • Affinity chromatography of adrenergic receptors and binding proteins. 
  • Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures. 
  • Biological activity of agarose-immobilized catecholamines. 
  • Biotinylated tags for recovery and characterization of ribonucleoprotein complexes. 
  • Bone sialoprotein-collagen interaction promotes hydroxyapatite nucleation. 
  • Cellular fatty acid synthase is required for late stages of HIV-1 replication. 
  • Chemoproteomic characterization of protein kinase inhibitors using immobilized ATP. 
  • Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds. 
  • Cocultures of adult and juvenile chondrocytes compared with adult and juvenile chondral fragments: in vitro matrix production. 
  • Comparative analysis of the ATP-binding sites of Hsp90 by nucleotide affinity cleavage: a distinct nucleotide specificity of the C-terminal ATP-binding site. 
  • Discovery of novel targets of quinoline drugs in the human purine binding proteome. 
  • Dorsal root ganglia neurite extension is inhibited by mechanical and chondroitin sulfate-rich interfaces. 
  • Fingerprinting polysaccharides with single-molecule atomic force microscopy. 
  • Fluorescence photobleaching with spatial Fourier analysis: measurement of diffusion in light-scattering media. 
  • Gamma-phosphate-linked ATP-sepharose for the affinity purification of protein kinases. Rapid purification to homogeneity of skeletal muscle mitogen-activated protein kinase kinase. 
  • In situ gelling hydrogels for conformal repair of spinal cord defects, and local delivery of BDNF after spinal cord injury. 
  • Laminin oligopeptide derivatized agarose gels allow three-dimensional neurite extension in vitro. 
  • Large scale isolation of genes as DNA fragment lengths by continuous elution electrophoresis through an agarose matrix. 
  • Molecular diffusion in tissue-engineered cartilage constructs: effects of scaffold material, time, and culture conditions. 
  • Optimization and characterization of the capillary human tumor clonogenic cell assay. 
  • Pulsed-field gel electrophoresis. 
  • Purification and characterization of the human platelet alpha 2-adrenergic receptor. 
  • Purification of alpha-sarcin and an antifungal protein from Aspergillus giganteus by blue sepharose CL-6B affinity chromatography. 
  • Purification of the AMP-activated protein kinase on ATP-gamma-sepharose and analysis of its subunit structure. 
  • Site-specific analysis of protein S-acylation by resin-assisted capture. 
  • Spatial distribution and acute anti-inflammatory effects of Methylprednisolone after sustained local delivery to the contused spinal cord. 
  • Sustained in vivo gene delivery from agarose hydrogel prolongs nonviral gene expression in skin. 
  • Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel. 
  • Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose. 
  • TRPV4-mediated mechanotransduction regulates the metabolic response of chondrocytes to dynamic loading. 
  • The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold. 
  • The polarity and magnitude of ambient charge influences three-dimensional neurite extension from DRGs. 
  • The role of cell surface receptors in the activation of human B cells by phosphorothioate oligonucleotides. 
  • The third component of complement: covalent attachment of a radioactive sugar to the labile binding site of C3 via the alternative pathway. 
  • Use of Sepharose 4B for preparative scale fractionation of eukaryotic messenger RNA's.