Specimen Handling

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  Subject Areas on Research

  • A Guide for a Cardiovascular Genomics Biorepository: the CATHGEN Experience. 
  • A North American multilaboratory study of CD4 counts using flow cytometric panLeukogating (PLG): a NIAID-DAIDS Immunology Quality Assessment Program Study. 
  • A comparison of respondent-driven and venue-based sampling of female sex workers in Liuzhou, China. 
  • A flat capillary tube system for assessment of sperm movement in cervical mucus. 
  • A model for harmonizing flow cytometry in clinical trials. 
  • A new way to obtain contrast-free bile during ERCP. 
  • A public health approach to cervical cancer screening in Africa through community-based self-administered HPV testing and mobile treatment provision. 
  • A technique for serial spermatozoa collection in mice. 
  • Accuracy and cost-effectiveness of cervical cancer screening by high-risk human papillomavirus DNA testing of self-collected vaginal samples. 
  • Adequacy of urine cytology specimens: an assessment of collection techniques. 
  • American Association for Bronchology and Interventional Pulmonology (AABIP) Statement on the Use of Bronchoscopy and Respiratory Specimen Collection in Patients With Suspected or Confirmed COVID-19 Infection. 
  • Anal-rectal cytology: a review. 
  • Apparatus for long-term ventricular access in the awake canine. 
  • Application of biomarkers in the development of drugs intended for the treatment of osteoarthritis. 
  • Aspergillus DNA contamination in blood collection tubes. 
  • Assessment of Available Online Information About Nasopharyngeal Swab Testing in Patient Instructions for Sinus and Pituitary Surgery. 
  • Assessment of an Online Tool to Simulate the Effect of Pooled Testing for SARS-CoV-2 Detection in Asymptomatic and Symptomatic Populations. 
  • Biobanking for Metabolomics and Lipidomics in Precision Medicine. 
  • Biobanking of human pancreas cancer tissue: impact of ex-vivo procurement times on RNA quality. 
  • Biomarkers and personalised medicine in rheumatoid arthritis: a proposal for interactions between academia, industry and regulatory bodies. 
  • Biospecimens and biorepositories for the community pathologist. 
  • Blood Substrate Collection and Handling Procedures under Pseudo-Field Conditions: Evaluation of Suitability for Inflammatory Biomarker Measurement. 
  • Blood culture contamination with Enterococci and skin organisms: implications for surveillance definitions of primary bloodstream infections. 
  • Cardiovascular Metabolomics. 
  • Centralized biorepositories for genetic and genomic research. 
  • Cervical cancer screening through human papillomavirus testing in community health campaigns versus health facilities in rural western Kenya. 
  • Clinical, histopathologic, and molecular markers of prognosis: toward a new disease risk stratification system for medulloblastoma. 
  • Combined iDISCO and CUBIC tissue clearing and lightsheet microscopy for in toto analysis of the adult mouse ovary†. 
  • Comment on: "Efficacies and merits of the cotton swab technique for diagnosing tinea capitis in the pediatric population". 
  • Common methods for fecal sample storage in field studies yield consistent signatures of individual identity in microbiome sequencing data. 
  • Comparative analysis of ACTH and corticosterone sampling methods in rats. 
  • Comparison of real-time PCR and microscopy for malaria parasite detection in Malawian pregnant women. 
  • Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification. 
  • Contained indirect viable-cell membrane immunofluorescence microassay for surface antigen analysis of cells infected with hazardous viruses. 
  • Controlled clinical comparison of BacT/ALERT standard aerobic and standard anaerobic blood culture bottles inoculated directly or after transport in sodium polyanethol sulfonate tubes. 
  • Cost-effectiveness of collecting routine cytologic specimens during fiberoptic bronchoscopy for endoscopically visible lung tumor. 
  • Determination of CD4 and CD8 lymphocyte subsets by a new alternative fluorescence immunoassay. 
  • Determining population and developmental pharmacokinetics of metronidazole using plasma and dried blood spot samples from premature infants. 
  • Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays. 
  • Development of a contemporary globally diverse HIV viral panel by the EQAPOL program. 
  • Development of an integrated biospecimen bank and multidisciplinary clinical database for pancreatic cancer. 
  • Diagnostic vitrectomy in the management of ocular disease. 
  • Dialyzable calcium measured in 60 micro L of routinely processed serum by continuous-flow analysis with CO2/air segmentation. 
  • Different methods of sample preparation influence sensitivity of Mycobacterium tuberculosis and Borrelia burgdorferi PCR. 
  • Effect of agitation and terminal subcultures on yield and speed of detection of the Oxoid Signal blood culture system versus the BACTEC radiometric system. 
  • Effect of chemical preservation of urine on routine urinalysis and non-culture tests for bacteriuria. 
  • Effects of Patient Characteristics on Diagnostic Performance of Self-Collected Samples for SARS-CoV-2 Testing. 
  • Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss). 
  • Enriched brucella medium for storage and transport of cultures of Campylobacter fetus subsp. jejuni. 
  • Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials. 
  • Establishment of reference intervals for osteoarthritis-related soluble biomarkers: the FNIH/OARSI OA Biomarkers Consortium. 
  • Ethical issues in gamete preservation for children undergoing treatment for cancer. 
  • Ethics of postmortem sperm retrieval: ethics of sperm retrieval after death or persistent vegetative state. 
  • Evaluating the Use of Silicone Wristbands To Measure Personal Exposure to Brominated Flame Retardants. 
  • Evaluation of a dried blood spot HIV-1 RNA program for early infant diagnosis and viral load monitoring at rural and remote healthcare facilities. 
  • Evaluation of preservative fluid for urine collected for culture. 
  • Evaluation of the Affirm Ambient Temperature Transport System for the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species from vaginal fluid specimens. 
  • Evaluation of transport media for Campylobacter jejuni in human fecal specimens. 
  • Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1. 
  • Factors affecting the risk of blood bank specimen hemolysis. 
  • Field evaluation and comparison of five methods of sampling lead dust on carpets. 
  • Fluorescent viability stains overestimate chondrocyte viability in osteoarticular allografts. 
  • HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities. 
  • High-throughput pooling and real-time PCR-based strategy for malaria detection. 
  • Histologic considerations for individualized systemic therapy approaches for the management of non-small cell lung cancer. 
  • Histopathologic examination of material from angioplasty balloon catheters used in vivo in human coronary arteries. 
  • Human biospecimen research: experimental protocol and quality control tools. 
  • Human metapneumovirus, Peru. 
  • Immunocytochemical localisation of collagens (I-V) in the human iris. 
  • Impact of Specimen Heterogeneity on Biomarkers in Repository Samples from Patients with Acute Myeloid Leukemia: A SWOG Report. 
  • Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT. 
  • Impact of different clinical variables on pregnancy outcome following embryo cryopreservation. 
  • Implementation of Good Clinical Laboratory Practice (GCLP) guidelines within the External Quality Assurance Program Oversight Laboratory (EQAPOL). 
  • In Vitro Evaluation of the Fresenius Kabi CATSmart Autotransfusion System. 
  • In vitro pilot analysis of uniformity, circularity, and concentricity of DSAEK donor endothelial grafts prepared by a microkeratome. 
  • Increased sectioning of pathologic specimens with ductal carcinoma in situ of the breast: are there clinical consequences? 
  • Key Highlights for the College of American Pathology Statement on Collection and Handling of Thoracic Small Biopsy and Cytology Specimens for Ancillary Studies. 
  • Leukopak PBMC sample processing for preparing quality control material to support proficiency testing programs. 
  • Light and vision in the deep-sea benthos: I. Bioluminescence at 500-1000 m depth in the Bahamian islands. 
  • Light and vision in the deep-sea benthos: II. Vision in deep-sea crustaceans. 
  • Long-term stability at -20 °C of Aspergillus galactomannan in serum and bronchoalveolar lavage specimens. 
  • Long-term stability of microbiome diversity and composition in fecal samples stored in eNAT medium. 
  • Macroscale spatial variation in chronic wound microbiota: a cross-sectional study. 
  • Microtome shaving radiography: demonstration of loss of mammographic microcalcifications during histologic sectioning. 
  • Mitigation of variation observed in a peripheral blood mononuclear cell (PBMC) based HIV-1 neutralization assay by donor cell pooling. 
  • Multibow: digital spectral barcodes for cell tracing. 
  • Multisite comparison of methods for the quantitation of the surface expression of CD38 on CD8(+) T lymphocytes. The ACTG Advanced Flow Cytometry Focus Group. 
  • Noninvasive measurement of mucosal immunity in a free-ranging baboon population. 
  • Optimal methods for fluorescence and diffuse reflectance measurements of tissue biopsy samples. 
  • Optimal timing for elective egg freezing. 
  • Optimized lymphocyte protein extraction performed simultaneously with DNA and RNA isolation: application to the study of factors affecting DNA, RNA, and protein recovery from lymphocytes of the oldest individuals. 
  • Pathologists' slide storage: ice age technology comes to the rescue. 
  • Patient-care practices associated with an increased prevalence of hepatitis C virus infection among chronic hemodialysis patients. 
  • Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia. 
  • Policy guidelines for the utilization of formalin-fixed, paraffin-embedded tissue sections: the UNC SPORE experience. University of North Carolina Specialized Program of Research Excellence. 
  • Postmortem delay has minimal effect on brain RNA integrity. 
  • Predictors and patterns of participant adherence to a cortisol collection protocol. 
  • Proposed high-risk screening protocol for Fabry disease in patients with renal and vascular disease. 
  • Prospective studies of neck dissection specimens support preservation of sublevel IIB for laryngeal squamous carcinoma with clinically negative neck. 
  • Protocol for the examination of specimens from patients with tumors of the brain/spinal cord. 
  • Quantitative neuromorphometry using magnetic resonance histology. 
  • Racial and Ethnic Differences in Acceptability of Urine and Cervico-Vaginal Sample Self-Collection for HPV-Based Cervical Cancer Screening. 
  • Radiation Therapy Oncology Group. Research Plan 2002-2006. Tumor Utilization Committee. 
  • RePORT International: Advancing Tuberculosis Biomarker Research Through Global Collaboration. 
  • Recommendations from the international stroke genetics consortium, part 2: biological sample collection and storage. 
  • Recovery and expression of messenger RNA from postmortem human brain tissue. 
  • Recovery of live virus after storage at ambient temperature using ViveST™. 
  • Regenerating titanium ventricular assist device surfaces after gold/palladium coating for scanning electron microscopy. 
  • Reply to "Insufficient demonstration of long-term stability of Aspergillus galactomannan". 
  • Resolving the molecular structure of microtubules under physiological conditions with scanning force microscopy. 
  • Revival of Seeliger's historical 'Special Listeria Culture Collection'. 
  • Semen analysis. 
  • Semen characteristics of Vietnam veterans. 
  • Setting objective thresholds for rare event detection in flow cytometry. 
  • Simple fixation and storage protocol for preserving the internal structure of intact human donor lenses and extracted human nuclear cataract specimens. 
  • Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay. 
  • Simultaneous determination of alfentanil and midazolam in human plasma using liquid chromatography and tandem mass spectrometry. 
  • Spotiton: New features and applications. 
  • Standardization of cytokine flow cytometry assays. 
  • Statistical methods for the assessment of EQAPOL proficiency testing: ELISpot, Luminex, and Flow Cytometry. 
  • Stereoselective analysis of bupropion and hydroxybupropion in human plasma and urine by LC/MS/MS. 
  • Strategies for genotype generation. 
  • Successful laboratory growth and analysis of CUSA-obtained medulloblastoma samples. Technical note. 
  • Survival of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus spp. for an extended period of transport. 
  • Symptomatic epidural cerebrospinal fluid collection following lumbar puncture and aneurysm clipping. 
  • Technologic advances for evaluation of cervical cytology: is newer better? 
  • Telomerase activity in ductal carcinoma in situ and invasive breast cancer. 
  • Telomere length analysis from minimally-invasively collected samples: Methods development and meta-analysis of the validity of different sampling techniques: American Journal of Human Biology. 
  • Test Accuracy of Human Papillomavirus in Urine for Detection of Cervical Intraepithelial Neoplasia. 
  • The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved human peripheral blood mononuclear cells. 
  • The External Quality Assurance Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay. 
  • The Immunology Quality Assessment Proficiency Testing Program for CD3⁺4⁺ and CD3⁺8⁺ lymphocyte subsets: a ten year review via longitudinal mixed effects modeling. 
  • The complexities of Xpert® MTB/RIF interpretation. 
  • The effect of blood-drawing techniques and equipment on the hemolysis of ED laboratory blood samples. 
  • Tissue storage and primer selection influence pyrosequencing-based inferences of diversity and community composition of endolichenic and endophytic fungi. 
  • Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays. 
  • Ultra-rapid freezing of thin biological samples. 
  • Update of the guidelines for lupus anticoagulant detection. Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the International Society on Thrombosis and Haemostasis. 
  • Urge overkill: protecting deidentified human subjects at what price? 
  • Urine processing impacts uric acid level in HIV-infected adults: implications for diagnosing tenofovir-associated proximal tubulopathy. 
  • Use of sentinel laboratories by clinicians to evaluate potential bioterrorism and emerging infections. 
  • Usefulness of collecting routine cytologic specimens during fiberoptic bronchoscopy for endoscopically visible and nonvisible lung carcinoma. 
  • Vaginal Self-Sampling for Human Papillomavirus Infection as a Primary Cervical Cancer Screening Tool in a Haitian Population. 
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  Keywords of People

  • Cohen-Wolkowiez, Michael, Professor of Pediatrics, Pediatrics, Infectious Diseases
  • Ferrari, Guido, Professor in Surgery, Molecular Genetics and Microbiology
  • Johnson, G. Allan, Charles E. Putman University Distinguished Professor of Radiology, Biomedical Engineering
  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Weinhold, Kent James, Joseph W. and Dorothy W. Beard Distinguished Professor of Experimental Surgery, in the School of Medicine, Immunology