Spectrometry, Fluorescence

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  Subject Areas on Research

  • 7-Nitrobenz-2-oxa-1,3-diazole (NBD)--phallacidin: synthesis of a fluorescent actin probe. 
  • A dual-emissive-materials design concept enables tumour hypoxia imaging. 
  • A high-performance liquid chromatographic method to measure sphingosine 1-phosphate and related compounds from sphingosine kinase assays and other biological samples. 
  • A kinetic study of the binding of an ADP fluorescent analog to mitochondrial ATPase. 
  • A low-cost high-efficiency fiber-optic coupler for recording action potentials within the myocardial wall. 
  • A multimodal micro-optrode combining field and single unit recording, multispectral detection and photolabeling capabilities. 
  • A rapid fluorescence assay for FtsZ assembly indicates cooperative assembly with a dimer nucleus. 
  • A series of new organic-inorganic molybdenum arsenate complexes based on [(ZnO6)(As3O3)2Mo6O18]4- and [HxAs2Mo6O26](6-x)- clusters as SBUs. 
  • A stable dimer in the pH-induced equilibrium unfolding of the homo-hexameric enzyme 4-oxalocrotonate tautomerase (4-OT). 
  • A study of the binding of thiamine diphosphate and thiochrome diphosphate to the pyruvate dehydrogenase multienzyme complex. 
  • A study on the diagnosis of drowning by examination of lung chlorophyll(a) of planktons with a spectrofluorophotometer. 
  • Adenosine nucleotides and the regulation of GRP94-client protein interactions. 
  • Advances in quantitative UV-visible spectroscopy for clinical and pre-clinical application in cancer. 
  • Affinity of (alpha-P-borano)-NTP analogs to rabbit muscle pyruvate kinase. 
  • Amino acid sequences of pyridoxal 5'-phosphate binding sites and fluorescence resonance energy transfer in chicken liver fatty acid synthase. 
  • Amplified on-chip fluorescence detection of DNA hybridization by surface-initiated enzymatic polymerization. 
  • An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging. 
  • Analysis of a workplace air particulate sample by synchronous luminescence and room-temperature phosphorescence. 
  • Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor. 
  • Analysis of sphingoid bases and sphingoid base 1-phosphates by high-performance liquid chromatography. 
  • Analysis of the regulation of microtubule dynamics by interaction of RGSZ1 (RGS20) with the neuronal stathmin, SCG10. 
  • Analysis of total aerobic viable counts in raw fish by high-throughput optical oxygen respirometry. 
  • Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell. 
  • Apolipoprotein C-III/sphingomyelin recombinants: formation, isolation, and characterization. 
  • Assembly and molecular activities of the MutS tetramer. 
  • Association of molybdopterin guanine dinucleotide with Escherichia coli dimethyl sulfoxide reductase: effect of tungstate and a mob mutation. 
  • Autofluorescence and diffuse reflectance properties of malignant and benign breast tissues. 
  • Autofluorescence spectroscopy of normal and malignant human breast cell lines. 
  • Binding of the beta2 adrenergic receptor to N-ethylmaleimide-sensitive factor regulates receptor recycling. 
  • Binding stoichiometry and structural mapping of the epsilon polypeptide of chloroplast coupling factor 1. 
  • Biomedical applications of high-performance liquid chromatography-mass spectrometry with continuous-flow fast atom bombardment. 
  • Calcium-sensing receptor regulates stomatal closure through hydrogen peroxide and nitric oxide in response to extracellular calcium in Arabidopsis. 
  • Calculation on fluorescence resonance energy transfer on surfaces. 
  • Calmodulin mediates Ca2+ sensitivity of sodium channels. 
  • Characterization and quantitative analysis of single-walled carbon nanotubes in the aquatic environment using near-infrared fluorescence spectroscopy. 
  • Characterization of sulfhydryl groups on chloroplast coupling factor 1 exposed by heat activation. 
  • Characterization of the molybdenum cofactor of sulfite oxidase, xanthine, oxidase, and nitrate reductase. Identification of a pteridine as a structural component. 
  • Chemical cross-linking studies of chloroplast coupling factor 1. 
  • Chemical modification and fluorescence studies of chloroplast coupling factor. 
  • Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition. 
  • Clamp subunit dissociation dictates bacteriophage T4 DNA polymerase holoenzyme disassembly. 
  • Compact point-detection fluorescence spectroscopy system for quantifying intrinsic fluorescence redox ratio in brain cancer diagnostics. 
  • Comparative studies on the secondary structure of ovalbumin messenger RNA and its complementary DNA transcript. 
  • Comparison of multiexcitation fluorescence and diffuse reflectance spectroscopy for the diagnosis of breast cancer (March 2003). 
  • Conformational changes of FtsZ reported by tryptophan mutants. 
  • Conformational changes of beta-lactoglobulin induced by anionic phospholipid. 
  • Contribution of aerobic photoheterotrophic bacteria to the carbon cycle in the ocean. 
  • Correlation between membrane potential changes and superoxide production in human granulocytes stimulated by phorbol myristate acetate. Evidence for defective activation in chronic granulomatous disease. 
  • Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. 
  • Design of a calcium-binding protein with desired structure in a cell adhesion molecule. 
  • Development and validation of a cell-based fluorescent method for measuring antibody affinity. 
  • Development of a high-performance liquid chromatography assay and revision of kinetic parameters for the Staphylococcus aureus sortase transpeptidase SrtA. 
  • Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection. 
  • Diagnosis of breast cancer using fluorescence and diffuse reflectance spectroscopy: a Monte-Carlo-model-based approach. 
  • Differential specificities and simultaneous occupancy of human MutSalpha nucleotide binding sites. 
  • Dispersion multiplexing with broadband filtering for miniature spectrometers. 
  • Dissecting the order of bacteriophage T4 DNA polymerase holoenzyme assembly. 
  • Dissociation of bacteriophage T4 DNA polymerase and its processivity clamp after completion of Okazaki fragment synthesis. 
  • Domain interactions in oxidized and reduced forms of rat liver sulfite oxidase. 
  • Donor-bridge-acceptor energetics determine the distance dependence of electron tunneling in DNA. 
  • Dual-wavelength ratiometric fluorescence measurement of the membrane dipole potential. 
  • Effect of optical clearing agents on the in vivo optical properties of squamous epithelial tissue. 
  • Effects of dibromothymoquinone on mung bean mitochondrial electron transfer and membrane fluidity. 
  • Elucidation of a [4Fe-4S] cluster degradation pathway: rapid kinetic studies of the degradation of Chromatium vinosum HiPIP. 
  • Endoscopic fluorescence detection of high-grade dysplasia in Barrett's esophagus. 
  • Energy transfer assays of rat renal cortical endosomal fusion: evidence for superfusion. 
  • Equilibrium binding of nucleotides to beef heart mitochondrial adenosine triphosphatase. 
  • Evaluation of a Fully Automated Research Prototype for the Immediate Identification of Microorganisms from Positive Blood Cultures under Clinical Conditions. 
  • Evidence of Fe3+ interaction with the plug domain of the outer membrane transferrin receptor protein of Neisseria gonorrhoeae: implications for Fe transport. 
  • Extrinsic pathway proteolytic activity. 
  • Femtosecond pulse train shaping improves two-photon excited fluorescence measurements. 
  • Fluorescence detection of cationic amyloid fibrils in human semen. 
  • Fluorescence energy transfer between ligand binding sites on aspartate transcarbamylase. 
  • Fluorescence energy transfer between the thiamine diphosphate and flavine adenine dinucleotide binding sites on the pyruvate dehydrogenase multienzyme complex. 
  • Fluorescence energy transfer measurements between ligand binding sites of the pyruvate dehydrogenase multienzyme complex. 
  • Fluorescence energy transfer measurements in rabbit muscle phosphofructokinase. 
  • Fluorescence energy transfer measurements in the pyruvate dehydrogenase multienzyme complex from Escherichia coli with chemically modified lipoic acid. 
  • Fluorescence resonance energy transfer analysis of cell surface receptor interactions and signaling using spectral variants of the green fluorescent protein. 
  • Fluorescence studies of benzo-[a]-pyrene in liposome membrane systems. 
  • Fluorescence studies of chicken liver fatty acid synthase. Segmental flexibility and distance measurements. 
  • Fluorescence studies of the pyruvate dehydrogenase multienzyme complex from Escherichia coli. 
  • Fluorescence studies on the binding of reduced nicotinamide adenine dinucleotide phosphate to human hemoglobin A and its variant hemoglobin Providence. 
  • Fluorescent probes as a measure of conformational alterations induced by nucleophilic modification and proteolysis of bovine alpha 2-macroglobulin. 
  • Fluorescent properties of pyrene bound at specific acylation sites of chicken liver fatty acid synthase. 
  • Folding kinetics of a fluorescent variant of monomeric lambda repressor. 
  • Folding mechanism of a multiple independently-folding domain protein: double B domain of protein A. 
  • Formation and repair of 1,3-bis-(2-chloroethyl)-1-nitrosourea and cisplatin induced total genomic DNA interstrand crosslinks in human glioma cells. 
  • Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. 
  • H-Ras peptide and protein substrates bind protein farnesyltransferase as an ionized thiolate. 
  • Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration. 
  • Heavy-atom effect on room temperature phosphorimetry. 
  • High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts. 
  • High-throughput screen for inhibitors of 1-deoxy-d-xylulose 5-phosphate reductoisomerase by surrogate ligand competition. 
  • Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development. 
  • Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications. 
  • Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate. 
  • Identification of fluorescent beads using a coded aperture snapshot spectral imager. 
  • Identification of molybdopterin as the organic component of the tungsten cofactor in four enzymes from hyperthermophilic Archaea. 
  • Identification of the second chromophore of Escherichia coli and yeast DNA photolyases as 5,10-methenyltetrahydrofolate. 
  • In vitro incorporation of nascent molybdenum cofactor into human sulfite oxidase. 
  • In vitro reconstitution of nitrate reductase activity of the Neurospora crassa mutant nit-1: specific incorporation of molybdopterin. 
  • Independent analysis of bait region cleavage dependent and thiolester bond cleavage dependent conformational changes by cross-linking of alpha 2-macroglobulin with cis-dichlorodiammineplatinum(II) and dithiobis(succinimidyl propionate). 
  • Indirect fluorometric detection of tryptic digests separated by capillary zone electrophoresis. 
  • Influence of lipid composition on physical properties and peg-mediated fusion of curved and uncurved model membrane vesicles: "nature's own" fusogenic lipid bilayer. 
  • Influence of the extent of hybridization on the hydrodynamic radius of DNA-functionalized gold nanoparticles. 
  • Injectable protease-operated depots of glucagon-like peptide-1 provide extended and tunable glucose control. 
  • Inside-out Ca(2+) signalling prompted by STIM1 conformational switch. 
  • Instrumentation for multi-modal spectroscopic diagnosis of epithelial dysplasia. 
  • Interaction of photoreactive and fluorescent nucleotides with chloroplast coupling factor 1. 
  • Interaction of reduced nicotinamide adenine dinucleotide with beef heart s-malate dehydrogenase. 
  • Interaction of synthetic HA2 influenza fusion peptide analog with model membranes. 
  • Interaction of the Shiga-like toxin type 1 B-subunit with its carbohydrate receptor. 
  • Interactions of nicotinamide adenine dinucleotides with varied states and forms of hemoglobin. 
  • Investigation of fiber-optic probe designs for optical spectroscopic diagnosis of epithelial pre-cancers. 
  • Investigation of noncalcium interactions of fura-2 by classical and synchronous fluorescence spectroscopy. 
  • Investigation of quercetin binding sites on chloroplast coupling factor 1. 
  • Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis. 
  • Laser-induced fluorescence spectroscopy for in vivo diagnosis of non-melanoma skin cancers. 
  • Laser-induced fluorescence spectroscopy: somewhere over the rainbow. 
  • Laser-induced fluorescence studies of polycyclic aromatic hydrocarbons (PAH) vapors at high temperatures. 
  • Lateral diffusion in planar lipid bilayers. 
  • Lateral mobility of integral proteins in red blood cell tethers. 
  • Ligand-selective interactions of ER detected in living cells by fluorescence resonance energy transfer. 
  • Light and vision in the deep-sea benthos: I. Bioluminescence at 500-1000 m depth in the Bahamian islands. 
  • Lipopolysaccharide and phorbol esters induce differentiation but have opposite effects on phosphatidylinositol turnover and Ca2+ mobilization in 70Z/3 pre-B lymphocytes. 
  • MIBG inhibits respiration: potential for radio- and hyperthermic sensitization. 
  • Mapping of a ligand-binding site for the human thromboxane A2 receptor protein. 
  • Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics 
  • Mechanisms for regulation of calmodulin kinase IIalpha by Ca(2+)/calmodulin and autophosphorylation of threonine 286. 
  • Membrane-protein structural mapping of chloroplast coupling factor in asolectin vesicles. 
  • Mitochondrial NADH as the bellwether of tissue O2 delivery. 
  • Molecular mobility on the cell surface. 
  • Monitoring intracellular proteins using fluorescence techniques: from protein synthesis and localization to activity. 
  • Monte-Carlo-based model for the extraction of intrinsic fluorescence from turbid media. 
  • Multi-excitation Raman spectroscopy technique for fluorescence rejection. 
  • Multicolor in vitro translation. 
  • Multiplexed short tandem repeat polymorphisms of the Weber 8A set of markers using tailed primers and infrared fluorescence detection. 
  • Native fluorescence and mag-indo-1-protein interaction as tools for probing unfolding and refolding sequences of the bovine serum albumin subdomain in the presence of guanidine hydrochloride. 
  • Near-infrared optical imaging of B16 melanoma cells via low-density lipoprotein-mediated uptake and delivery of high emission dipole strength tris[(porphinato)zinc(II)] fluorophores. 
  • Non-invasive monitoring of intra-tumor drug concentration and therapeutic response using optical spectroscopy. 
  • Non-invasive, simultaneous quantification of vascular oxygenation and glucose uptake in tissue. 
  • Noncovalent functionalization of carbon nanotubes by fluorescein-polyethylene glycol: supramolecular conjugates with pH-dependent absorbance and fluorescence. 
  • Ocular albumin fluorophotometric quantitation of endotoxin-induced vascular permeability. 
  • Optical imaging of living brain slices. 
  • Optimal methods for fluorescence and diffuse reflectance measurements of tissue biopsy samples. 
  • Optimal visual perception and detection of oral cavity neoplasia. 
  • Orientation of LamB signal peptides in bilayers: influence of lipid probes on peptide binding and interpretation of fluorescence quenching data. 
  • Osmolyte-induced folding of an intrinsically disordered protein: folding mechanism in the absence of ligand. 
  • Palladin is an actin cross-linking protein that uses immunoglobulin-like domains to bind filamentous actin. 
  • Pathways of folding of reduced bovine pancreatic ribonuclease. 
  • Peroxynitrite formation in nitric oxide-exposed submitochondrial particles: detection, oxidative damage and catalytic removal by Mn-porphyrins. 
  • Phenylalanine hydroxylase activity in foetal hepatic organ culture 
  • Phospholipid-dextran with a single coupling point: a useful amphiphile for functionalization of nanomaterials. 
  • Phosphorylation of RNA polymerase II CTD fragments results in tight binding to the WW domain from the yeast prolyl isomerase Ess1. 
  • Phosphorylation of an alpha-synuclein peptide fragment enhances metal binding. 
  • Probing the renin active site by collisional quenching of endogenous fluorescence. 
  • Protein-protein and protein-DNA interactions at the bacteriophage T4 DNA replication fork. Characterization of a fluorescently labeled DNA polymerase sliding clamp. 
  • Purification and activity study of the A- and B-chains of cinnamomin, a type II ribosome-inactivating protein. 
  • Quantitative analysis of twelve sulfonamides in honey after acidic hydrolysis by high-performance liquid chromatography with post-column derivatization and fluorescence detection. 
  • Quantitative and selective fluorophore labeling of phosphoserine on peptides and proteins: characterization at the attomole level by capillary electrophoresis and laser-induced fluorescence. 
  • Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo. 
  • Quantum dots-bienzyme hybrid system for the sensitive determination of glucose. 
  • Recovery of rat adrenal amine stores after insulin administration. 
  • Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. IV. The Escherichia coli hemoflavoprotein: subunit structure and dissociation into hemoprotein and flavoprotein components. 
  • Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. 
  • Role of lysophospholipid growth factors in the modulation of aqueous humor outflow facility. 
  • Rotational dynamics of chloroplast ATP synthase in phospholipid vesicles. 
  • Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces. 
  • Sensing membrane stress with near IR-emissive porphyrins. 
  • Sensitive analysis of [D-Pen2,5]enkephalin in rat serum by capillary electrophoresis and laser-induced fluorescence detection. 
  • Sensitive, label-free protein assay using 1-ethyl-3-methylimidazolium tetrafluoroborate-supported microchip electrophoresis with laser-induced fluorescence detection. 
  • Simultaneous optical recording of membrane potential and intracellular calcium from brain slices. 
  • Sliding clamp of the bacteriophage T4 polymerase has open and closed subunit interfaces in solution. 
  • Spatial relationships between specific sites on reconstituted chloroplast proton adenosinetriphosphatase and the phospholipid vesicle surface. 
  • Spectral filtering modulation method for estimation of hemoglobin concentration and oxygenation based on a single fluorescence emission spectrum in tissue phantoms. 
  • Spectroscopic diagnosis of esophageal cancer: new classification model, improved measurement system. 
  • Stimulation of the T3-T cell receptor complex induces a membrane-potential-sensitive calcium influx. 
  • Structural and mechanistic studies of the alpha-ketoglutarate dehydrogenase multienzyme complex from Escherichia coli. 
  • Structural and metabolic relationship between the molybdenum cofactor and urothione. 
  • Structural investigation of reconstituted chloroplast ATPase with fluorescence measurements. 
  • Structural map of the dicyclohexylcarbodiimide site of chloroplast coupling factor determined by resonance energy transfer. 
  • Structural mapping of aspartate transcarbamoylase by fluorescence energy-transfer measurements: determination of the distance between catalytic sites of different subunits. 
  • Structural mapping of chloroplast coupling factor. 
  • Structural mapping of rabbit muscle phosphofructokinase. Distance between the adenosine cyclic 3',5'-phosphate binding site and a reactive sulfhydryl group. 
  • Structural organization of chloroplast coupling factor. 
  • Structural organization of the human insulin receptor ectodomain. 
  • Structure-activity relationships for the reserpine-like actions of derivatives of beta-carboline in vitro. 
  • Subunit dissociation and unfolding of rabbit muscle phosphofructokinase by guanidine by hydrochloride. 
  • Synchronous luminescence: a new detection technique for multiple fluorescent probes used for DNA sequencing. 
  • Synthesis and characterization of fluoropolymeric substrata with immobilized minimal peptide sequences for cell adhesion studies. I. 
  • Temperature triggered self-assembly of polypeptides into multivalent spherical micelles. 
  • Temporal sequence and spatial distribution of early events of fertilization in single sea urchin eggs. 
  • The CD28 signaling pathway regulates glucose metabolism. 
  • The MotA transcriptional activator of bacteriophage T4 binds to its specific DNA site as a monomer. 
  • The SIT image Vidicon as a gas-phase fluorescence detector for gas chromatography. 
  • The effect of divalent cations on the conformation and function of human plasminogen. 
  • The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus. 
  • The pterin component of the molybdenum cofactor. Structural characterization of two fluorescent derivatives. 
  • The role of cavitation in acoustically activated drug delivery. 
  • Three-dimensional reconstruction in free-space whole-body fluorescence tomography of mice using optically reconstructed surface and atlas anatomy. 
  • Time-Resolved Synchronous Fluorescence for Biomedical Diagnosis. 
  • Time-resolved investigation of bright visible wavelength luminescence from sulfur-doped ZnO nanowires and micropowders. 
  • Toward the detection of cellular copper(II) by a light-activated fluorescence increase. 
  • Tracking and quantification of single-walled carbon nanotubes in fish using near infrared fluorescence. 
  • Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy. 
  • Transient opening of fibronectin type III (FNIII) domains: the interaction of the third FNIII domain of FN with anastellin. 
  • Ultrafast singlet excited-state polarization in electronically asymmetric ethyne-bridged bis[(porphinato)zinc(II)] complexes. 
  • Use of a multiseparation fiber optic probe for the optical diagnosis of breast cancer. 
  • pH dependence of adenosine 5'-triphosphate synthesis and hydrolysis catalyzed by reconstituted chloroplast coupling factor. 
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  Keywords of People

  • Badea, Cristian Tudorel, Professor in Radiology, Biomedical Engineering
  • Erickson, Harold Paul, James B. Duke Professor of Cell Biology, Biomedical Engineering
  • Ferguson, P. Lee, Associate Professor of Civil and Environmental Engineering, Civil and Environmental Engineering
  • Johnson, G. Allan, Charles E. Putman University Professor of Radiology, Physics
  • Oas, Terrence Gilbert, Professor of Biochemistry, Duke Cancer Institute
  • Palmer, Gregory M., Associate Professor of Radiation Oncology, Radiation Oncology
  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology