Spectrophotometry

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  Subject Areas on Research

  • A CORRELATION BETWEEN THE PROTEIN STRUCTURE AND CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE. 
  • A cellular Trojan Horse for delivery of therapeutic nanoparticles into tumors. 
  • A kinetic investigation of the interaction of alpha-methylaspartic acid with aspartate aminotransferase. 
  • A kinetic investigation of the interaction of erythro-beta-hydroxyaspartic acid with aspartate aminotransferase. 
  • A new purification procedure for bovine milk xanthine oxidase: effect of proteolysis on the subunit structure. 
  • A novel eye in 'eyeless' shrimp from hydrothermal vents of the Mid-Atlantic Ridge. 
  • A series of new organic-inorganic molybdenum arsenate complexes based on [(ZnO6)(As3O3)2Mo6O18]4- and [HxAs2Mo6O26](6-x)- clusters as SBUs. 
  • A simplified method for monitoring the cytochrome aa3 redox state in bilateral cortical areas of unanesthetized cats. 
  • A structural model of the alternative oxidase of plant mitochondria. 
  • A temperature jump study of aspartate aminotransferase. a reinvestigation. 
  • Age-dependent photoionization thresholds of melanosomes and lipofuscin isolated from human retinal pigment epithelium cells. 
  • Altered ultrastructural morphology of self-aggregated low density lipoproteins: coalescence of lipid domains forming droplets and vesicles. 
  • An active site tyrosine influences the ability of the dimethyl sulfoxide reductase family of molybdopterin enzymes to reduce S-oxides. 
  • An investigation of hemopexin redox properties by spectroelectrochemistry: biological relevance for heme uptake. 
  • An iron tetrahydroporphyrin prosthetic group common to both assimilatory and dissimilatory sulfite reductases. 
  • An obligately photosynthetic bacterial anaerobe from a deep-sea hydrothermal vent. 
  • Anaerobic oxidations of myoglobin and hemoglobin by spectroelectrochemistry. 
  • Analysis of slitless holographic spectrometers implemented by spherical beam volume holograms. 
  • Assay and detection of the molybdenum cofactor. 
  • Assessment of the number of nucleotide binding sites on chloroplast coupling factor 1 by the continuous variation method. 
  • Automated analysis for free and short-chain acylcarnitine in plasma with a centrifugal analyzer. 
  • Automated dry-film method for phosphorus in serum evaluated. 
  • Binding of phosphate ligands to ribonuclease A. 
  • Biological evaluation of some norphenyl analogs of hemicholinium No. 3 and acetyl-seco-hemicholinium No. 3. 
  • Bis(2,2'-bipyridine)(5,6-epoxy-5,6-dihydro-[1,10]phenanthroline)ruthenium: synthesis and electrochemical and electrochemiluminescence characterization. 
  • Calcium influx and poststimulus current during early adaptation in Aplysia giant neurons. 
  • Carbon monoxide-cytochrome interactions in the brain of the fluorocarbon-perfused rat. 
  • Carbon monoxide-driven reduction of ferric heme and heme proteins. 
  • Cerebral cytochrome a,a3 inhibition by cyanide in bloodless rats. 
  • Characterization of a spectrophotometric assay for cAMP phosphodiesterase. 
  • Characterization of the aqueous iron(III) chelation chemistry of a potential Trojan Horse antimicrobial agent: chelate structure, stability and pH dependent speciation. 
  • Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrome P-450 reductase. 
  • Characterization of the molybdenum cofactor of sulfite oxidase, xanthine, oxidase, and nitrate reductase. Identification of a pteridine as a structural component. 
  • Chemical modification and site-directed mutagenesis of conserved HXXH and PP-loop motif arginines and histidines in the murine bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase. 
  • Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition. 
  • Chromogenic assay for phospholipase C from Bacillus cereus. 
  • Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein. 
  • Comparison of a chemical assay with a tissue-mediated bio-assay for chemical mutagens. 
  • Conformational changes in the photocycle of Anabaena sensory rhodopsin: absence of the Schiff base counterion protonation signal. 
  • Conformational changes of beta-lactoglobulin induced by anionic phospholipid. 
  • Cyclophilin active site mutants have native prolyl isomerase activity with a protein substrate. 
  • Detection, characterization, and abundance of engineered nanoparticles in complex waters by hyperspectral imagery with enhanced Darkfield microscopy. 
  • Determination of residual manganese in Mn porphyrin-based superoxide dismutase (SOD) and peroxynitrite reductase mimics. 
  • Dialyzable calcium measured in 60 micro L of routinely processed serum by continuous-flow analysis with CO2/air segmentation. 
  • Differential replication of satellite DNA in polyploid tissues of Drosophila virilis. 
  • Digital image analysis for rapid quantification of total RNA and cDNA for SMART-PCR. 
  • Direct Optical Imaging of Graphene In Vitro by Nonlinear Femtosecond Laser Spectral Reshaping 
  • Dispersion multiplexing with broadband filtering for miniature spectrometers. 
  • Distance dependence of electron transfer in rigid, cofacially compressed, pi-stacked porphyrin-bridge-quinone systems. 
  • Distribution and tissue uptake of magnesium related to its pharmacological effects. 
  • Duchenne muscular dystrophy: detection of deletion carriers by spectrophotometric densitometry. 
  • Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. VI. Newly synthesized proteins in the T4 protein-deoxyribonucleic acid complex. 
  • Effect of chemical modification of dihydrouridine in yeast transfer ribonucleic acid on amino acid acceptor activity and ribosomal binding. 
  • Effect of conformation on the binding of flavins to flavoenzymes. 
  • Effect of pH and anions on functional properties of hemoglobin from Lemur fulvus fulvus. 
  • Effect of steady illumination on the binding of carbon monoxide by carboxymethylated cytochrome c. 
  • Effect of transient hypoxia on oxygenation of the developing rat brain: relationships among haemoglobin saturation, autoregulation of blood flow and mitochondrial redox state. 
  • Effects of S-nitrosation on oxygen binding by normal and sickle cell hemoglobin. 
  • Effects of serum creatinine calibration on estimated renal function in african americans: the Jackson heart study. 
  • Electromagnetic spectroscopy of normal breast tissue specimens obtained from reduction surgeries: comparison of optical and microwave properties. 
  • Electron microscopic study of the base sequence in nucleic acids. VI. Preparation of ribonucleic acid with marked gauanosine monophosphate nucleotides. 
  • Electron paramagnetic resonance and optical evidence for interaction between siroheme and Fe4S4 prosthetic groups in complexes of Escherichia coli sulfite reductase hemoprotein with added ligands. 
  • Evaluation of BA1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy. 
  • Evidence against formation of A23187 dimers and oligomers in solution: photo-induced degradation of Ionophore A23187. 
  • Evidence for siroheme-Fe4S4 interaction in spinach ferredoxin-sulfite reductase. 
  • Fluorescence energy transfer measurements in rabbit muscle phosphofructokinase. 
  • Functional and structural properties of Murex fulvescens hemocyanin: isolation of two different subunits required for reassociation of a molluscan hemocyanin. 
  • Functional consequences of ligand-linked dissociation in hemoglobin from the sea cucumber Molpadia arenicola. 
  • Gamma-L-glutaminyl-3,4-benzoquinone. Structural studies and enzymatic synthesis. 
  • Genetic diversity of coastal bottlenose dolphins revealed by structurally and functionally diverse hemoglobins. 
  • HEPATIC ALDEHYDE OXIDASE. 3. THE SUBSTRATE-BINDING SITE. 
  • HEPATIC ALDEHYDE OXIDASE. II. DIFFERENTIAL INHIBITION OF ELECTRON TRANSFER TO VARIOUS ELECTRON ACCEPTORS. 
  • Heme-copper oxidases use tunneling pathways. 
  • Hemoglobin-based oxygen carriers (HBOCs): structural alterations that affect free radical generation. 
  • Hepatic sulfite oxidase. Congruency in mitochondria of prosthetic groups and activity. 
  • Hepatic sulfite oxidase. Identification of the molybdenum center as the site of irreversible inactivation by ferricyanide. 
  • Histologic, nuclear DNA, and human papillomavirus studies of cervical condylomas. 
  • Human erythrocyte catalase: an improved method of isolation and a reevaluation of reported properties. 
  • Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum. 
  • Identification of quinone-binding and heme-ligating residues of the smallest membrane-anchoring subunit (QPs3) of bovine heart mitochondrial succinate:ubiquinone reductase. 
  • In vitro effects of fluoride and bromide on pseudocholinesterase and acetylcholinesterase activities. 
  • Incorporation of tyrosine and glutamine residues into the soluble guanylate cyclase heme distal pocket alters NO and O2 binding. 
  • Induction of specific cytochrome P-450 isozymes by methylenedioxyphenyl compounds and antagonism by 3-methylcholanthrene. 
  • Integrated optical attenuated total reflection spectrometry of aqueous superstrates using prism-coupled polymer waveguides. 
  • Interaction of photoreactive and fluorescent nucleotides with chloroplast coupling factor 1. 
  • Interconversion of red opsin isoforms by the cyclophilin-related chaperone protein Ran-binding protein 2. 
  • Intracellular monitoring of experimental respiratory failure. Collaborative Group on Intracellular Monitoring. 
  • Intraocular pressure effects on optic nerve-head oxidative metabolism measured in vivo. 
  • Intravitreal clearance of tissue plasminogen activator in the rabbit. 
  • Investigation of quercetin binding sites on chloroplast coupling factor 1. 
  • Investigation of the influence on conformational transition of DNA induced by cationic lipid vesicles. 
  • Isolation and characterization of the major apolipoprotein from chicken high density lipoproteins. 
  • Isolation and characterization of two group-specific antigens from feline leukemia virus. 
  • Isolation from the mushroom Agaricus bisporus and chemical synthesis of gamma-L-glutaminyl-4-hydroxybenzene. 
  • Isolation of the domain containing the molybdenum, iron-sulfur I, and iron-sulfur II centers of chicken liver xanthine dehydrogenase. 
  • Kinetic and equilibrium studies of the ribonuclease-catalyzed hydrolysis of uridine 2',3'-cyclic phosphate. 
  • Kinetic mechanism of quinone oxidoreductase 2 and its inhibition by the antimalarial quinolines. 
  • Kinetic studies of tryptophan synthetase. Interaction of L-serine, indole, and tryptophan with the native enzyme. 
  • Kinetic studies of tryptophan synthetase. Interaction of substrates with the B subunit. 
  • Kinetics of iron release from ferric binding protein (FbpA): mechanistic implications in bacterial periplasm-to-cytosol Fe3+ transport. 
  • Label free colorimetric biosensing using nanoparticles. 
  • Localization of a cryptic binding site for tenascin on fibronectin. 
  • Lysolipid incorporation in dipalmitoylphosphatidylcholine bilayer membranes enhances the ion permeability and drug release rates at the membrane phase transition. 
  • MR imaging of experimental and clinical thrombi at 1.5 T. 
  • Mapping tertiary interactions in protein folding reactions: a novel mass spectrometry- and chemical synthesis-based approach. 
  • Measuring morphological features using light-scattering spectroscopy and Fourier-domain low-coherence interferometry. 
  • Mechanism of action of a respiratory inhibitor from the gill tissue of the sporulating common mushroom, Agaricus bisporus. 
  • Microassay for the photometric quantitation of cell-associated plasminogen activator using a chromogenic tripeptide substrate. 
  • Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes. 
  • Mitoxantrone and ametantrone inhibit hydroperoxide-dependent initiation and propagation reactions in fatty acid peroxidation. 
  • Mn porphyrin-based superoxide dismutase (SOD) mimic, MnIIITE-2-PyP5+, targets mouse heart mitochondria. 
  • Molecular basis of the biological function of molybdenum. Molybdenum-free sulfite oxidase from livers of tungsten-treated rats. 
  • Molecular basis of the biological function of molybdenum. Molybdenum-free xanthine oxidase from livers of tungsten-treated rats. 
  • Molybdopterin guanine dinucleotide: a modified form of molybdopterin identified in the molybdenum cofactor of dimethyl sulfoxide reductase from Rhodobacter sphaeroides forma specialis denitrificans. 
  • Molybdopterin in carbon monoxide oxidase from carboxydotrophic bacteria. 
  • NO+, NO, and NO- donation by S-nitrosothiols: implications for regulation of physiological functions by S-nitrosylation and acceleration of disulfide formation. 
  • Neutron-stimulated emission computed tomography of a multi-element phantom. 
  • Nitric oxide binding to human ferrihemoglobins cross-linked between either alpha or beta subunits. 
  • Nitric oxide interactions with cobalamins: biochemical and functional consequences. 
  • Nitrosative stress: metabolic pathway involving the flavohemoglobin. 
  • Nitrosylation of manganese(II) tetrakis(N-ethylpyridinium-2-yl)porphyrin: a simple and sensitive spectrophotometric assay for nitric oxide. 
  • Nonequivalence of the flavin adenine dinucleotide moieties of chicken liver xanthine dehydrogenase. 
  • Oligonucleotides produced by digestion of bakers' yeast serine transfer ribonucleic acid with specific nucleases. 
  • On the feasibility of comprehensive high-resolution 3D remote dosimetry. 
  • On the polyol synthesis of silver nanostructures: glycolaldehyde as a reducing agent. 
  • On the reaction of ferric heme proteins with nitrite and sulfite. 
  • Optical and electron paramagnetic resonance spectroscopic studies on purine hydroxylase II from Aspergillus nidulans. 
  • Optical imaging of epileptiform activity in human neocortex. 
  • Optimization of expression of human sulfite oxidase and its molybdenum domain. 
  • Parathyroid hormone secretion and target organ response in experimental acute pancreatitis. 
  • Partial restoration of normal functional properties in carboxypeptidase A-digested hemoglobin. 
  • Performance comparison of aperture codes for multimodal, multiplex spectroscopy. 
  • Phase-lifetime spectrophotometry of deoxycholate-purified bacteriorhodopsin reconstituted into asolectin vesicles. 
  • Phase-lifetime spectrophotometry of membranes from ion flux mutants of Halobacterium halobium. 
  • Phosphate ester hydrolysis is catalyzed by a bacterial transferrin: potential implications for in vivo iron transport mechanisms. 
  • Polynitrosylated proteins: characterization, bioactivity, and functional consequences. 
  • Polypeptides of avian RNA tumor viruses. 1. Isolation and physical and chemical analysis. 
  • Polypeptides of avian RNA tumor viruses. V. Analysis of the virus core. 
  • Pressure corrections for CoCl2 as a thermometer in an analytic ultracentrifuge. 
  • Properties of rabbit muscle phosphofructokinase modified with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole. 
  • Protein nitration is mediated by heme and free metals through Fenton-type chemistry: an alternative to the NO/O2- reaction. 
  • Protonation state of Glu142 differs in the green- and blue-absorbing variants of proteorhodopsin. 
  • Purification and characterization of a lecithin-D(-)- -hydroxybutyrate dehydrogenase complex. 
  • Purification and properties of chicken liver xanthine dehydrogenase. 
  • Purification and properties of the NAD+-dependent (type D) and O2-dependent (type O) forms of rat liver xanthine dehydrogenase. 
  • Purification and properties of xanthine dehydroganase from Micrococcus lactilyticus. 
  • Purification of serine-specific transfer ribonucleic acid from bakers' yeast. 
  • Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins. 
  • Quantification of mouse macrophage chemotaxis in vitro: role of C5 for the production of chemotactic activity. 
  • RNA extraction from human articular cartilage by chondrocyte isolation. 
  • Reactions of manganese porphyrins with peroxynitrite and carbonate radical anion. 
  • Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. 3. The Escherichia coli hemoflavoprotein: catalytic parameters and the sequence of electron flow. 
  • Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. IV. The Escherichia coli hemoflavoprotein: subunit structure and dissociation into hemoprotein and flavoprotein components. 
  • Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. V. Studies with the Escherichia coli hemoflavoprotein depleted of flavin mononucleotide: distinct roles for the flavin adenine dinucleotide and flavin mononucleotide prosthetic groups in catalysis. 
  • Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. VI. The reaction of carbon monoxide with the Escherichia coli holoenzyme, the hemoprotein, and free siroheme. 
  • Reflectance spectroscopy. 
  • Regulation of translation and transcription of messenger RNA during early embryonic development. 
  • Relaxation spectra of aspartate transcarbamylase. I. Interaction of 5-bromocytidine triphosphate with native enzyme and regulatory subunit. 
  • Relaxation spectra of aspartate transcarbamylase. Interaction of the catalytic subunit with carbamyl phosphate, succinate, and L-malate. 
  • Relaxation spectra of aspartate transcarbamylase. Interaction of the native enzyme with aspartate analogs. 
  • Relaxation spectra of aspartate transcarbamylase. Interaction of the native enzyme with carbamyl phosphate. 
  • Relaxation spectra of ribonuclease. VII. The interaction of ribonuclease with uridine 2',3'-cyclic phosphate. 
  • Resonance Raman spectroscopic characterization of the molybdopterin active site of DMSO reductase. 
  • Ring-Opening Copolymerization of Maleic Anhydride with Functional Epoxides: Poly(propylene fumarate) Analogues Capable of Post-Polymerization Modification. 
  • Robust IR-based detection of stable and fractionally populated G-C+ and A-T Hoogsteen base pairs in duplex DNA. 
  • Role of conserved tyrosine 343 in intramolecular electron transfer in human sulfite oxidase. 
  • Scanning fiber angle-resolved low coherence interferometry. 
  • Shape and size control of ZnO nanostructures via a simple solution route. 
  • Simultaneous measurement of cytochrome a3 reduction and chemoreceptor afferent activity in the carotid body. 
  • Single-target molecule detection with nonbleaching multicolor optical immunolabels. 
  • Siroheme and sirohydrochlorin. The basis for a new type of porphyrin-related prosthetic group common to both assimilatory and dissimilatory sulfite reductases. 
  • Siroheme: a new prosthetic group participating in six-electron reduction reactions catalyzed by both sulfite and nitrite reductases. 
  • Siroheme: a prosthetic group of the Neurospora crassa assimilatory nitrite reductase. 
  • Site-directed mutagenesis of recombinant sulfite oxidase: identification of cysteine 207 as a ligand of molybdenum. 
  • Slow oscillations of cytochrome oxidase redox state and blood volume in unanesthetized cat and rabbit cortex. Interhemispheric synchrony. 
  • Spectral perturbations and oligomer/monomer formation in 124-kilodalton Avena phytochrome. 
  • Spectrophotometry of b-type cytochromes in rat brain in vivo and in vitro. 
  • Spectroscopic evidence for anthracenedione antineoplastic agent self-association and complex formation with flavin nucleotides. 
  • Spectroscopic studies of the molybdenum-containing dimethyl sulfoxide reductase from Rhodobacter sphaeroides f. sp. denitrificans. 
  • Spot hemoglobin. Studies on the Root effect hemoglobin of a marine teleost. 
  • Structural and functional properties of cytochrome c oxidases isolated from sharks. 
  • Structural and metabolic relationship between the molybdenum cofactor and urothione. 
  • Structural studies of the heme domain of sulfite oxidase: CNBr fragments. 
  • Structure and phase behavior of lipid suspensions containing phospholipids with covalently attached poly(ethylene glycol). 
  • Structure of macromolecular aggregates. I. Aggregation-induced conformational changes in polypeptides. 
  • Studies of aldehyde oxidase by electron paramagnetic resonance spectroscopy. II. Kinetic studies by rapid freezing. 
  • Studies on photosystem I. I. Relationship of plastocyanin, cytochrome f and P700. 
  • Studies on photosystem I. II. Involvement of ferredoxin in cyclic electron flow. 
  • Studies on the dissociation of flavin adenine dinucleotide from metalloflavoproteins. 
  • Superoxide dismutase from Streptococcus mutans. Isolation and characterization of two forms of the enzyme. 
  • Superoxidized states of Escherichia coli sulfite reductase heme protein subunit. 
  • Synthesis and iron sequestration equilibria of novel exocyclic 3-hydroxy-2-pyridinone donor group siderophore mimics. 
  • THE ABSORPTION SPECTRA OF IRON-FLAVOPROTEINS. 
  • The case of the missing NO-hemoglobin: spectral changes suggestive of heme redox reactions reflect changes in NO-heme geometry. 
  • The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex. 
  • The effect of detergents on the reduction of tetrazolium salts. 
  • The growth mechanism of copper nanowires and their properties in flexible, transparent conducting films. 
  • The hemoglobin of the crossopterygian fish, Latimeria chalumnae (Smith). Subunit structure and oxygen equilibria. 
  • The interaction of aspartate aminotransferase with alpha-methylaspartic acid. 
  • The isolation of 20alpha- and 20beta-hydroxypregn-4-en-3-one from porcine corpora lutea. 
  • The kinetics of intramolecular distribution of 15N in uric acid following administration of 15N-glycine: preferential labeling of N-(3+9) of uric acid in primary gout and a reappraisal of the "glutamine hypothesis". 
  • The location of the carotenoid in the B800--850 light-harvesting pigment--protein complex from Rhodopseudomonas capsulata. 
  • The ortho effect makes manganese(III) meso-tetrakis(N-methylpyridinium-2-yl)porphyrin a powerful and potentially useful superoxide dismutase mimic. 
  • The oxyhemoglobin reaction of nitric oxide. 
  • The partially reduced species present in purified cytochrome oxidase from baker's yeast is cytochrome a. 
  • The pterin component of the molybdenum cofactor. Structural characterization of two fluorescent derivatives. 
  • The site of the redox-linked proton pump in eukaryotic cytochrome c oxidases. 
  • The state of reduction of molybdopterin in xanthine oxidase and sulfite oxidase. 
  • The use of diaminobenzidine for spectrophotometric and acrylamide gel detection of sulfite oxidase and its applicability to hydrogen peroxide-generating enzymes. 
  • Thermodynamic parameters of the alpha-methyl aspartate-aspartate aminotransferase interaction. 
  • Thin-layer methods for determination of oxygen binding curves of hemoglobin solutions and blood. 
  • Thrombogenesis: effect of molecular motions, surface order, and hydrophobicity of the polymer interface. 
  • Total reconstitution of copper-zinc superoxide dismutase. 
  • Tryptic cleavage of rat liver sulfite oxidase. Isolation and characterization of molybdenum and heme domains. 
  • Urinary metabolities of harmine in the rat and their inhibition of monoamine oxidase. 
  • Use of intrinsic optical signals to monitor physiological changes in brain tissue slices. 
  • Using optical spectroscopy to longitudinally monitor physiological changes within solid tumors. 
  • Vertical distribution and characterization of aerobic phototrophic bacteria at the Juan de Fuca Ridge in the Pacific Ocean. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor of Cell Biology, Cell Biology
  • Palmer, Gregory M., Associate Professor of Radiation Oncology, Radiation Oncology
  • Turner, Dennis Alan, Professor of Neurosurgery, Biomedical Engineering