Spectrophotometry, Ultraviolet

• 

  Subject Areas on Research

  • 3D-printed microfluidic device for the detection of pathogenic bacteria using size-based separation in helical channel with trapezoid cross-section. 
  • A colloidal Au monolayer modulates the conformation and orientation of a protein at the electrode/solution interface. 
  • A continuously monitored spectrophotometric assay of glycosidases with nitrophenyl glycosides. 
  • A kit method for the high level synthesis of [211At]MABG. 
  • A new SOD mimic, Mn(III) ortho N-butoxyethylpyridylporphyrin, combines superb potency and lipophilicity with low toxicity. 
  • A new method for studying the interaction between chlorpromazine and phospholipid bilayer. 
  • A new purification procedure for bovine milk xanthine oxidase: effect of proteolysis on the subunit structure. 
  • A pro-chelator triggered by hydrogen peroxide inhibits iron-promoted hydroxyl radical formation. 
  • A prochelator activated by hydrogen peroxide prevents metal-induced amyloid Beta aggregation. 
  • A prochelator with a modular masking group featuring hydrogen peroxide activation with concurrent fluorescent reporting. 
  • A rapid method for the isolation and partial purification of specific eucaryotic messenger RNA's. 
  • A whole blood immunoassay using gold nanoshells. 
  • Acid-base and electrochemical properties of manganese meso(ortho- and meta-N-ethylpyridyl)porphyrins: potentiometric, spectrophotometric and spectroelectrochemical study of protolytic and redox equilibria. 
  • Advances in quantitative UV-visible spectroscopy for clinical and pre-clinical application in cancer. 
  • Allosteric actuation of inverse phase transition of a stimulus-responsive fusion polypeptide by ligand binding. 
  • An equilibrium binding study of the interaction of aspartate transcarbamylase with cytidine 5'-triphosphate and adenosine 5'-triphosphate. 
  • An iron tetrahydroporphyrin prosthetic group common to both assimilatory and dissimilatory sulfite reductases. 
  • Antigens from synthetic and natural estrogens: formation by conjugation with protein through a thiopropionic acid link. 
  • Application of analytical high-speed counter-current chromatography to the isolation of bioactive natural products. 
  • Assembly of microtubules from preformed, ring-shaped protofilaments and 6-S tubulin. 
  • Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity. 
  • Base stacking and molecular polarizability: effect of a methyl group in the 5-position of pyrimidines. 
  • Biochemical properties of oncornavirus polypeptides. 
  • Biomedical applications of high-performance liquid chromatography-mass spectrometry with continuous-flow fast atom bombardment. 
  • Boron-containing oligodeoxyribonucleotide 14mer duplexes: enzymatic synthesis and melting studies. 
  • CO binding by hemocyanins of Limulus polyphemus, Busycon carica, and Callinectes sapidus. 
  • Camptothecin analogues with enhanced antitumor activity at acidic pH. 
  • Chaperone-like activity of alpha-crystallin. The effect of NADPH on its interaction with zeta-crystallin. 
  • Characterisation of PduS, the pdu metabolosome corrin reductase, and evidence of substructural organisation within the bacterial microcompartment. 
  • Combining 'caged-dopamine' photolysis with fast-scan cyclic voltammetry to assess dopamine clearance and release autoinhibition in vitro. 
  • Comparison of multiexcitation fluorescence and diffuse reflectance spectroscopy for the diagnosis of breast cancer (March 2003). 
  • Comprehensive two-dimensional high-performance liquid chromatography for the isolation of overexpressed proteins and proteome mapping. 
  • Computational de novo design and characterization of a four-helix bundle protein that selectively binds a nonbiological cofactor. 
  • Conformational studies of the nucleic acid binding sites for Xenopus transcription factor IIIA. 
  • Conformational transition of DNA induced by cationic lipid vesicle in acidic solution: spectroscopy investigation. 
  • Control of the orientational order and nonlinear optical response of the "push-pull" chromophore RuPZn via specific incorporation into densely packed monolayer ensembles of an amphiphilic four-helix bundle peptide: characterization of the peptide-chromophore complexes. 
  • Coordination chemistry of siderophores: thermodynamics and kinetics of iron chelation and release. 
  • Copper-chelatin: isolation from various eucaryotic sources. 
  • Counterions influence reactivity of metal ions with cysteinyldopa model compounds. 
  • Crystal structure of a photoactive yellow protein from a sensor histidine kinase: conformational variability and signal transduction. 
  • De novo design of a single-chain diphenylporphyrin metalloprotein. 
  • Delivery of antisense oligonucleotides using HPMA polymer: synthesis of A thiol polymer and its conjugation to water-soluble molecules. 
  • Deoxyribonucleic acid ligase. Isolation and physical characterization of the homogeneous enzyme from Escherichia coli. 
  • Detection and characterization of a glutathione conjugate of ochratoxin A. 
  • Determination of separate inhibitor and substrate binding sites in the dehaloperoxidase-hemoglobin from Amphitrite ornata. 
  • Development and validation of a liquid chromatographic method for the determination of the related substances of ramipril in Altace capsules. 
  • Diagnosis of breast cancer using diffuse reflectance spectroscopy: Comparison of a Monte Carlo versus partial least squares analysis based feature extraction technique. 
  • Didodecyldimethylammonium bromide lipid bilayer-protected gold nanoparticles: synthesis, characterization, and self-assembly. 
  • Differential effects of nitric oxide-mediated S-nitrosylation on p50 and c-jun DNA binding. 
  • Disposition of amiodarone and its proximate metabolite, desethylamiodarone, in the dog for oral administration of single-dose and short-term drug regimens. 
  • Disruption of the toxic conformation of the expanded polyglutamine stretch leads to suppression of aggregate formation and cytotoxicity. 
  • Drug targeting using thermally responsive polymers and local hyperthermia. 
  • Effect of colloidal gold size on the conformational changes of adsorbed cytochrome c: probing by circular dichroism, UV-visible, and infrared spectroscopy. 
  • Effect of electrode surface microstructure on electron transfer induced conformation changes in cytochrome c monitored by in situ UV and CD spectroelectrochemistry. 
  • Effect of freeze-thawing on lipid bilayer-protected gold nanoparticles. 
  • Effect of pH and anions on functional properties of hemoglobin from Lemur fulvus fulvus. 
  • Effects of pharmaceutically active compounds on a mixed microbial community originating from a municipal wastewater treatment plant. 
  • Electrochemical detection of cobalt in hair following on-column derivatization with 1-(2-pyridylazo)-2-naphthol-6-sulphonic acid and reversed-phase liquid chromatography. 
  • Electrochemistry and spectroscopy study on the interaction of microperoxidase-11 with lipid membrane. 
  • Equilibrium binding of nucleotides to beef heart mitochondrial adenosine triphosphatase. 
  • Evidence against formation of A23187 dimers and oligomers in solution: photo-induced degradation of Ionophore A23187. 
  • Fe(III) coordination properties of two new saccharide-based enterobactin analogues: methyl 2,3,4-tris-O-[N-[2,3-di(hydroxy)benzoyl-glycyl]-aminopropyl]-alpha-D-glucopyranoside and methyl 2,3,4-tris-O-[N-[2,3-di-(hydroxy)-benzoyl]-aminopropyl]-alpha-D-glucopyranoside. 
  • Fe(III)-complexes of the tripodal trishydroxamate siderophore basidiochrome: potential biological implications. 
  • Flow linear dichroism and electron microscopic analysis of protein-DNA complexes of a mutant UvrB protein that binds to but cannot kink DNA. 
  • Fluorescence energy transfer measurements in rabbit muscle phosphofructokinase. 
  • Ga3+ as a mechanistic probe in Fe3+ transport: characterization of Ga3+ interaction with FbpA. 
  • Glutathione-associated cis-diamminedichloroplatinum(II) metabolism and ATP-dependent efflux from leukemia cells. Molecular characterization of glutathione-platinum complex and its biological significance. 
  • Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells. 
  • Gold-nanocrystal-enhanced bioluminescent nanocapsules 
  • Hepatic sulfite oxidase. Identification of the molybdenum center as the site of irreversible inactivation by ferricyanide. 
  • High-performance liquid chromatographic analysis for a non-chromophore-containing phosphatidyl inositol analog, 1-((1-O-octadecyl-2-O-methyl-sn-glycero)phospho)-1D-3-deoxy-myo-inositol, using indirect UV detection. 
  • Highly sensitive methods for assaying the enzymes of vitamin B6 metabolism. 
  • Human Factor XIII from plasma and platelets. Molecular weights, subunit structures, proteolytic activation, and cross-linking of fibrinogen and fibrin. 
  • Hydrolysis of N3-methyl-2'-deoxycytidine: model compound for reactivity of protonated cytosine residues in DNA. 
  • Identification and quantification of regioisomeric cholesteryl linoleate hydroperoxides in oxidized human low density lipoprotein and high density lipoprotein. 
  • Identification of impurities in erythromycin by liquid chromatography-mass spectrometric detection. 
  • Identification of molybdopterin as the organic component of the tungsten cofactor in four enzymes from hyperthermophilic Archaea. 
  • Identification of the second chromophore of Escherichia coli and yeast DNA photolyases as 5,10-methenyltetrahydrofolate. 
  • Improved synthesis of N-succinimidyl 4-[18F]fluorobenzoate and its application to the labeling of a monoclonal antibody fragment. 
  • Incorporation of designed extended chromophores into amphiphilic 4-helix bundle peptides for nonlinear optical biomolecular materials. 
  • Inhaled azithromycin therapy. 
  • Interaction of aspartate transcarbamylase with 5-bromocytidine 5'-tri-, di-, and monophosphates. 
  • Interfacial phase transition of an environmentally responsive elastin biopolymer adsorbed on functionalized gold nanoparticles studied by colloidal surface plasmon resonance. 
  • Investigation of quercetin binding sites on chloroplast coupling factor 1. 
  • Iron chelation properties of an extracellular siderophore exochelin MN. 
  • Isolation and characterization of a second molybdopterin dinucleotide: molybdopterin cytosine dinucleotide. 
  • Isolation of proteins by gel filtration in 6M guanidinium chloride: application to RNA tumor viruses. 
  • LC-UV/MS methods for the analysis of prochelator-boronyl salicylaldehyde isonicotinoyl hydrazone (BSIH) and its active chelator salicylaldehyde isonicotinoyl hydrazone (SIH). 
  • Liposome-mediated conformation transition of DNA detected by molecular probe: methyl green. 
  • Metal-induced formation of metallothionein in rat liver. 
  • Modulating unimolecular charge transfer by exciting bridge vibrations. 
  • Molecular basis of the biological function of molybdenum. Molybdenum-free sulfite oxidase from livers of tungsten-treated rats. 
  • Molecular basis of the biological function of molybdenum. Molybdenum-free xanthine oxidase from livers of tungsten-treated rats. 
  • Monte Carlo-based inverse model for calculating tissue optical properties. Part II: Application to breast cancer diagnosis. 
  • Neutral proteinase of rabbit skin: an enzyme capable of degrading skin protein and inducing an inflammatory response. 
  • New PEG-ylated Mn(III) porphyrins approaching catalytic activity of SOD enzyme. 
  • New class of potent catalysts of O2.-dismutation. Mn(III) ortho-methoxyethylpyridyl- and di-ortho-methoxyethylimidazolylporphyrins. 
  • Noninvasive monitoring of tissue hemoglobin using UV-VIS diffuse reflectance spectroscopy: a pilot study. 
  • Optical detection of polycations via polymer film-modified microtiter plates: response mechanism and bioanalytical applications. 
  • Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples. 
  • Optimization of the lariat ether carboxylic acid host structure for ferrioxamine B: demonstration of a second coordination shell chelate effect. 
  • Optimized production and analysis of the staphylococcal multidrug efflux protein QacA. 
  • Oxidation of molybdopterin in sulfite oxidase by ferricyanide. Effect on electron transfer activities. 
  • Paired-ion liquid chromatographic method for the analysis of a phenanthrenemethanol antimalarial in whole blood. 
  • Pathways of folding of reduced bovine pancreatic ribonuclease. 
  • Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937. 
  • Photoionization thresholds of melanins obtained from free electron laser-photoelectron emission microscopy, femtosecond transient absorption spectroscopy and electron paramagnetic resonance measurements of oxygen photoconsumption. 
  • Physical and chemical properties of rabbit muscle phosphofructokinase cross-linked with dimethyl suberimidate. 
  • Physical properties of human alpha 2-macroglobulin following reaction with methylamine and trypsin. 
  • Portable UV-visible spectrometer for measuring absorbance and dichroism of Langmuir monolayers at air-water interfaces. 
  • Preferential synthesis of yeast mitochondrial DNA in alpha factor-arrested cells. 
  • Preparative solvent partition chromatography of butyrylated cyclic AMP analogues. 
  • Probing near-infrared photorelaxation pathways in eumelanins and pheomelanins. 
  • Prochelator BHAPI protects cells against paraquat-induced damage by ROS-triggered iron chelation. 
  • Prochelators triggered by hydrogen peroxide provide hexadentate iron coordination to impede oxidative stress. 
  • Properties of rabbit muscle phosphofructokinase modified with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole. 
  • Pure manganese(III) 5,10,15,20-tetrakis(4-benzoic acid)porphyrin (MnTBAP) is not a superoxide dismutase mimic in aqueous systems: a case of structure-activity relationship as a watchdog mechanism in experimental therapeutics and biology. 
  • Purification and characterization of the Cu,Zn SOD from Escherichia coli. 
  • Purification and some properties of Cd-binding protein from rat liver. 
  • Quality of potent Mn porphyrin-based SOD mimics and peroxynitrite scavengers for pre-clinical mechanistic/therapeutic purposes. 
  • Rapid separation and characterization of protein and peptide mixtures using 1.5 microns diameter non-porous silica in packed capillary liquid chromatography/mass spectrometry. 
  • Reagents for astatination of biomolecules: comparison of the in vivo distribution and stability of some radioiodinated/astatinated benzamidyl and nido-carboranyl compounds. 
  • Real-time visualization of diffusion-controlled nanowire growth in solution. 
  • Relaxation spectra of aspartate transcarbamylase. Interaction of the native enzyme with an adenosine 5'-triphosphate analog. 
  • Rotational isomers of N-alkylpyridylporphyrins and their metal complexes. HPLC separation, (1)H NMR and X-ray structural characterization, electrochemistry, and catalysis of O(2)(.-) disproportionation. 
  • SOD-like activity of Mn(II) beta-octabromo-meso-tetrakis(N-methylpyridinium-3-yl)porphyrin equals that of the enzyme itself. 
  • Secondary structure of ovalbumin messenger RNA. 
  • Silver nanoparticle-alginate composite beads for point-of-use drinking water disinfection. 
  • Simple method of measurement of orotic acid and orotidine in urine. 
  • Single-handed helical wrapping of single-walled carbon nanotubes by chiral, ionic, semiconducting polymers. 
  • Siroheme and sirohydrochlorin. The basis for a new type of porphyrin-related prosthetic group common to both assimilatory and dissimilatory sulfite reductases. 
  • Siroheme: a prosthetic group of the Neurospora crassa assimilatory nitrite reductase. 
  • Soluble guanylate cyclase is activated differently by excess NO and by YC-1: resonance Raman spectroscopic evidence. 
  • Specific and non-specific measurements of tissue angiotensin II cascade members. 
  • Spectrophotometric analysis of molecular transport in gels. 
  • Stoichiometric preference in copper-promoted oxidative DNA damage by ochratoxin A. 
  • Structural characterization of a molybdopterin precursor. 
  • Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors. 
  • Structure of a G.T.A triplet in an intramolecular DNA triplex. 
  • Studies of the copper and heme cofactors of pseudomonad L-tryptophan-2,3-dioxygenase by electron paramagnetic resonance spectroscopy. 
  • Subunit composition of high molecular weight oligomer: Limulus polyphemus hemocyanin. 
  • Tetrahydrobiopterin rapidly reduces the SOD mimic Mn(III) ortho-tetrakis(N-ethylpyridinium-2-yl)porphyrin. 
  • The effect of plasmin on the subunit structure of human fibrin. 
  • The structure of a molybdopterin precursor. Characterization of a stable, oxidized derivative. 
  • Thermal stability of the prototypical Mn porphyrin-based superoxide dismutase mimic and potent oxidative-stress redox modulator Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin chloride, MnTE-2-PyP(5+). 
  • Thermodynamics, kinetics, and mechanism of the stepwise dissociation and formation of Tris(L-lysinehydroxamato)iron(III) in aqueous acid. 
  • Toward the development of prochelators as fluorescent probes of copper-mediated oxidative stress. 
  • Tryptic cleavage of rat liver sulfite oxidase. Isolation and characterization of molybdenum and heme domains. 
  • Two-photon absorption properties of proquinoidal D-A-D and A-D-A quadrupolar chromophores. 
  • Understanding the role of oxidative etching in the polyol synthesis of Pd nanoparticles with uniform shape and size. 
  • Using alpha-helical coiled-coils to design nanostructured metalloporphyrin arrays. 
  • Validation of the use of 6,6'-dithiodinicotinic acid as a chromogen in the Ellman method for cholinesterase determinations. 
  • Very high pressure gradient LC/MS/MS. 
  • Where Is the Electronic Oscillator Strength? Mapping Oscillator Strength across Molecular Absorption Spectra. 
  • [Endogenous inhibitors of the specific binding of benzodiazepines in the cerebral cortex]. 
  • cis-parthenolid-9-one from Anvillea garcinii. 
• 

  Keywords of People

  • Katz, David F., Nello L. Teer, Jr. Distinguished Professor of Biomedical Engineering, in the Edmund T. Pratt, Jr. School of Engineering, Biomedical Engineering
  • Palmer, Gregory M., Associate Professor of Radiation Oncology, Radiation Oncology